LOCUS       sequence-319131-       10185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration Vector, assembled with pHIE047 (TUPV1), pJM451 (TUPV2), 
            pPD612.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10185)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 10185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pHIE063 ZF43x3-S mKate2 EF1a EBFP2-P2A-BlastR
FEATURES             Location/Qualifiers
     source          1..10185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1031..1068
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1079..1678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1079..1098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1575..1595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1791..1810)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1856..1911
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1910..1929)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     regulatory      3129..3138
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3135..3833
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    3884..4108
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5157..6339
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5388..6330
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6287..6307
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     6341..6365
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6440..6449
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6446..6448,6449..6451,6452..7162)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(6491..6512)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             7190..7246
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7259..7657
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7708..7932
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(8791..9600)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9514..9533
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9592..9611
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(join(9759..10185,1..162))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9839..9858)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
       61 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      241 ttatcaaaaa ggatcttcac ctagatcctt tctcgagctt tcggagtgag atggagtggc
      301 cactgtggaa ctgggcctag catcgcgata agctctagag ggacagcccc cccccaaagc
      361 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
      421 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
      481 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
      541 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
      601 cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt gatatcgaat
      661 tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
      721 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
      781 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
      841 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
      901 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
      961 gcgcgcctgg tggcgaacga tcaacagacg ggagatctac tattgggaca gcagagatcc
     1021 agttggtaat ggcttgtcga cgacggcggt ctccgtcgtc aggatcatag gtgccaccat
     1081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     1141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     1201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1561 gacatccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc
     1681 ttccactgga ttgtacaatt accaactgaa ttcactcctc aggtgcaggc tgcctatcag
     1741 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     1801 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     1861 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     1921 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     1981 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     2041 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     2101 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     2161 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     2221 cttctcttat ggagatccgg gcctagcatc gcgataagct ctagagggac agcccccccc
     2281 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     2341 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     2401 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     2461 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     2521 gaatgacaga atcatagaac ggcctgcgta cggatggggc tctagctcta gagcttgata
     2581 tcgaattcct gcagccccgc tagagggaca gccccccccc aaagccccca gggatgtaat
     2641 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     2701 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     2761 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     2821 ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg
     2881 gcctgggcgc gcctggtggc gaacgatcaa cagacgggag attgccaagg tcttgacaaa
     2941 cgagtgctag tacaagatcg cgtcgacgga tcgggagatc tgaattagag tgaggacaat
     3001 cgtatacaag tagatccaga gtgaggacta cctatagaag taagagtgag gacaatcgta
     3061 tacaagtaga tccctctaga gggtatataa tgggggccac tagtctacta ccagagctca
     3121 tcgctagcgc caccatggtg agcgagctga ttaaggagaa catgcacatg aagctgtata
     3181 tggagggcac cgtgaacaac caccacttca agtgtacctc cgagggcgaa ggcaagccct
     3241 acgagggcac ccagaccatg agaatcaagg cggtcgaggg cggccctctc cccttcgcct
     3301 tcgacatcct ggctaccagc ttcatgtacg gcagcaaaac cttcatcaac cacacccagg
     3361 gcatccccga cttctttaag cagtccttcc ccgagggctt cacatgggag agagtcacca
     3421 catacgaaga tgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc
     3481 tcatctacaa cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga
     3541 agaaaacact cggctgggag gcctccaccg aaaccctgta ccccgctgac ggcggcctgg
     3601 aaggcagagc cgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttga
     3661 aaaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactatg
     3721 tggacaggag actggaaaga atcaaggagg ccgacaaaga aacctacgtc gagcagcacg
     3781 aggtggctgt ggccagatac tgcgacctcc ctagcaaact ggggcacaga tgagcggccg
     3841 ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     3901 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     3961 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     4021 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaagt caatagcagg
     4081 catgctgggg atgcggtggg ctctatggct tctgaggcag gtcgagggta catgcgcctt
     4141 actccttgtg agcgcatcgc gataagctct agagggacag ccccccccca aagcccccag
     4201 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     4261 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     4321 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     4381 cacctggggg gatacgggga aaaagcttta ggctgaaaga gagatttaga atgacagaat
     4441 catagaacgg cctgcgtacg gatggggctc tagctctaga gcttgatatc gaattcctgc
     4501 agccccgcta gagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc
     4561 ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca
     4621 tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct
     4681 ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa
     4741 aaagctttag gctgaaagag agatttagaa tgacagaatc atagaacggc ctgggcgcgc
     4801 ctggtggcga acgatcaaca gacgggagag ggcaaaaccg atgttgacgg actaatcctg
     4861 acaagatctg gggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     4921 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     4981 gatgtacggg ccagatatac gcgtgagtaa ttcatacaaa aggactcgcc cctgccttgg
     5041 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     5101 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     5161 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     5221 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     5281 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     5341 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     5401 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     5461 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     5521 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     5581 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     5641 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     5701 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     5761 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     5821 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     5881 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     5941 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     6001 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     6061 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     6121 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     6181 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     6241 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     6301 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag agctcgttta gtgaaccgtc
     6361 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagagaa cgggaccgat
     6421 ccaaattctc gaggctagcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     6481 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgagggg
     6541 cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg aagttcatct gcaccaccgg
     6601 caagctgccc gtgccctggc ccaccctcgt gaccaccctg agccacggcg tgcagtgctt
     6661 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     6721 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc acctacaaga cccgcgccga
     6781 ggtgaagttc gagggcgaca ccctagtgaa ccgcatcgag ctgaagggcg tcgacttcaa
     6841 ggaggacggc aacatcctgg ggcacaagct ggagtacaac ttcaacagcc acaacatcta
     6901 tatcatggcc gtcaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacgt
     6961 ggaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     7021 ccccgtgctg ctgcccgaca gccactacct gagcacccag tccgtgctga gcaaagaccc
     7081 caacgagaag cgcgatcaca tggtcctgct ggagttccgc accgccgccg ggatcactct
     7141 cggcatggac gagctgtaca aggaattcgg tagcaagctt ggtagtggag caactaactt
     7201 ttccctgctc aaacaagccg gggatgtaga ggagaacccc ggccccgccc cccttaagat
     7261 ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa
     7321 cagcatcccc atctctgagg actacagcgt cgccagcgca gctctctcta gcgacggccg
     7381 catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt
     7441 gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa
     7501 tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca
     7561 tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg
     7621 tgaattgctg ccctctggtt atgtgtggga gggctaagcg gccgctcgag tctagagggc
     7681 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     7741 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     7801 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     7861 tggggcagga cagcaagggg gaggattggg aagtcaatag caggcatgct ggggatgcgg
     7921 tgggctctat ggcttctgag gcaggtcgaa gaactatttc ctggctgtta cgcgagcgca
     7981 tcgcgataag ctctagaggg acagcccccc cccaaagccc ccagggatgt aattacgtcc
     8041 ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc
     8101 ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga
     8161 tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg
     8221 gggaaaaagc tttaggctga aagagagatt tagaatgaca gaatcataga acggcctgcg
     8281 tacggatggg gctctagctc tagagcttga tatcgaattc ctgcagcccc gctagaggga
     8341 cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca
     8401 gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc
     8461 gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc
     8521 tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagct ttaggctgaa
     8581 agagagattt agaatgacag aatcatagaa cggcctgggc gcgcctggtg gcgaacgatc
     8641 aacagacggg agaggactag aggatgcaca tgtgaccgag ggatggaacg acgcgtaact
     8701 cacgttaagg gattttggtc atgagcttgc gccgtcccgt caagtcagcg taatgctctg
     8761 ccagtgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa
     8821 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     8881 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     8941 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     9001 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
     9061 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     9121 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     9181 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     9241 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgtttttcc
     9301 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     9361 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     9421 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     9481 gcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     9541 atcagcatcc atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat
     9601 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
     9661 tttatcttgt gcaatgtaac atcagagatt ttgagacacg ggccatgcta gctccagcaa
     9721 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9781 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9841 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9901 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9961 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    10021 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    10081 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    10141 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actac
//
