LOCUS       sequence-319133-       17427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PP7-tagged reporter driven by the Arabidopsis GAPC2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17427)
  AUTHORS   Alamos S, Reimer A, Niyogi KK, Garcia HG
  TITLE     Quantitative imaging of RNA polymerase II activity in plants reveals
            the single-cell basis of tissue-wide transcriptional dynamics.
  JOURNAL   Nat Plants. 2021 Aug;7(8):1037-1049. doi: 
            10.1038/s41477-021-00976-0. Epub 2021 Aug 9.
  PUBMED    34373604
REFERENCE   2  (bases 1 to 17427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-00976-0"; journalName: "Nat Plants"; date: 
            "2021-08"; volume: "7"; issue: "8"; pages: "1037-1049"
COMMENT     Sequence Label: AL13Rb-GAPC2
FEATURES             Location/Qualifiers
     source          1..17427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    571..595
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     783..805
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     797..814
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     798..814
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          5052..5240
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     terminator      6669..6916
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             8268..8963
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     8669..8688
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(9568..9584)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9568..9584)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9581..9603)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9592..9608
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9616..9622,9623..9640,9641..9646))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9661..9682
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9873..10549
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10486..10506
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             10612..11409
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10669..10688)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11279..11298
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11466..11640
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11718..11742
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12167..12961
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13048..13636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13537..13556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13790..13807
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    13822..13962
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(13888..13907)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14048..14070
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      14306..14500
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14566..15639)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(16068..16697)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
       61 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
      121 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
      181 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
      241 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
      301 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
      361 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
      421 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
      481 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
      541 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
      601 tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      661 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      721 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      781 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtcga
      841 ctggctgaca cttgacgggt cagttataac ttataagtgg actcttggaa atgatcatga
      901 gttatagaaa gatcacaaac ttcaatttat gatatattgt tgacctcaga taatatttta
      961 gctatttatt ggatgtataa agatccatat gaataccaaa agaaggagtt ttataaccat
     1021 tcacctaaca aaaatgaaat ggaaaaagaa aagagagcaa ataagaggag gagaagaaga
     1081 agacttttgg caaaagccaa gagtcatacc tattagtcta ttacataagg tgtgatgttt
     1141 ttttttgctg tcacattcca tctgacctac caaagctctc aaagtggagc atctattgtg
     1201 tcttggtata gagtcacgat tagataaata tacaacacca tatgtacttg tgtcaaatta
     1261 taaatatttg aaacagtaag caaccagtaa ttcgataaag acgtctacaa gcatctacac
     1321 atctaaattg attaaaacaa aacaccttta aatatagtgt ttatgaagtc aaatcttcac
     1381 cttgattatt ctaaaacaat ggttggagta atgttgctga atgttgagtg agaattgaca
     1441 tttgagagta actttcacta taatgaactg gatgataaag ttgatccgat aataattgaa
     1501 tttaccatga atggtatcac aagatacatg tatagaagac agtggtgtta cttgttacgc
     1561 aaaattaaaa aattagcata ctattgcagt tactttggat ttattaagga aaattatggt
     1621 ttgacaacaa caataataca aaatcttatg aaaattaaat aaaaagaaaa acaaatttgg
     1681 ctattggcaa agctcattgg ctgtcaaaag gaatatatac aaaatctgct acgttgcagt
     1741 cttgcgtgca ccggcccagc ccataggatt agagtattaa acctcgaata ttccatcagc
     1801 ctgcgcgtga atccaagcgc cattagtttc cccaaatcag ttcttaatcc tacccataaa
     1861 cgatgggtaa aaatggtaaa taagaaagaa agtaaagtac aatatagtaa tattaattag
     1921 tgaatctaat ctattagcct ttttcccaag aaaaaatctc agccactcga tcatattttc
     1981 aattttcatc atcacgttct tcttctcttt taaataaccc taaatcctca ccaaacccaa
     2041 accctcactc actattttca cattctcttc tctctcgata tcatctaaat ctctctcgat
     2101 ctcaatttcg caaagggatc ctacggtact tattgccaag aaagcacgag acgatatggc
     2161 gtccgtgcct ccaggtcgaa tcttcaaacg acgagaggat atggcctccg tcgctccagt
     2221 attccagggt tcatcagatc ctacggtact tattgccaag aaagcacgag acgatatggc
     2281 gtccgtgcct ccaggtcgaa tcttcaaacg acgagaggat atggcctccg tcgctccagt
     2341 attccagggt tcatcagatc ctacggtact tattgccaag aaagcacgag acgatatggc
     2401 gtccgtgcct ccaggtcgaa tcttcaaacg acgagaggat atggcctccg tcgctccagt
     2461 attccagggt tcatcagatc ctacggtact tattgccaag aaagcacgag acgatatggc
     2521 gtccgtgcct ccaggtcgaa tcttcaaacg acgagaggat atggcctccg tcgctccagt
     2581 attccagggt tcatcagatc ctacggtact tattgccaag aaagcacgag acgatatggc
     2641 gtccgtgcct ccaggtcgaa tcttcaaacg acgagaggat atggcctccg tcgctccagt
     2701 attccagggt tcatcagatc ctacggtact tattgccaag aaagcacgag acgatatggc
     2761 gtccgtgcct ccaggtcgaa tcttcaaacg acgagaggat atggcctccg tcgctccagt
     2821 attccagggt tcatcagatc ctacggtact tattgccaag aaagcacgag acgatatggc
     2881 gtccgtgcct ccaggtcgaa tcttcaaacg acgagaggat atggcctccg tcgctccagt
     2941 attccagggt tcatcagatc ctacggtact tattgccaag aaagcacgag acgatatggc
     3001 gtccgtgcct ccaggtcgaa tcttcaaacg acgagaggat atggcctccg tcgctccagt
     3061 attccagggt tcatcagatc ctacggtact tattgccaag aaagcacgag acgatatggc
     3121 gtccgtgcct ccaggtcgaa tcttcaaacg acgagaggat atggcctccg tcgctccagt
     3181 attccagggt tcatcagatc ctacggtact tattgccaag aaagcacgag acgatatggc
     3241 gtccgtgcct ccaggtcgaa tcttcaaacg acgagaggat atggcctccg tcgctccagt
     3301 attccagggt tcatcagatc ctacggtact tattgccaag aaagcacgag acgatatggc
     3361 gtccgtgcct ccaggtcgaa tcttcaaacg acgagaggat atggcctccg tcgctccagt
     3421 attccagggt tcatcagatc ctacggtact tattgccaag aaagcacgag acgatatggc
     3481 gtccgtgcct ccaggtcgaa tcttcaaacg acgagaggat atggcctccg tcgctccagt
     3541 attccagggt tcatcagagt gccagagtcc ttcgataggg acaagacaat tgcactgatc
     3601 atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc
     3661 tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
     3721 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
     3781 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttctgagg
     3841 agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc
     3901 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt
     3961 ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt
     4021 atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
     4081 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
     4141 ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct
     4201 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
     4261 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatcgtt
     4321 gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa
     4381 tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct tcccgacgat
     4441 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
     4501 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
     4561 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
     4621 agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgtatgg tccgtcctgt
     4681 agaaacccca acccgtgaaa tcaaaaaact cgacggcctg tgggcattca gtctggatcg
     4741 cgaaaactgt ggaattggtc agcgttggtg ggaaagcgcg ttacaagaaa gccgggcaat
     4801 tgctgtgcca ggcagtttta acgatcagtt cgccgatgca gatattcgta attatgcggg
     4861 caacgtctgg tatcagcgcg aagtctttat accgaaaggt tgggcaggcc agcgtatcgt
     4921 gctgcgtttc gatgcggtca ctcattacgg caaagtgtgg gtcaataatc aggaagtgat
     4981 ggagcatcag ggcggctata cgccatttga agccgatgtc acgccgtatg ttattgccgg
     5041 gaaaagtcta cgtaagtttc tgcttctacc tttgatatat atataataat tatcattaat
     5101 tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag tatatagcaa
     5161 ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct aatatatgac
     5221 caaaatttgt tgatgtgcag gtatcaccgt ttgtgtgaac aacgaactga actggcagac
     5281 tatcccgccg ggaatggtga ttaccgacga aaacggcaag aaaaagcagt cttacttcca
     5341 tgatttcttt aactatgccg gaatccatcg cagcgtaatg ctctacacca cgccgaacac
     5401 ctgggtggac gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt
     5461 tgactggcag gtggtggcca atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca
     5521 ggtggttgca actggacaag gcactagcgg gactttgcaa gtggtgaatc cgcacctctg
     5581 gcaaccgggt gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc agacagagtg
     5641 tgatatctac ccgcttcgcg tcggcatccg gtcagtggca gtgaagggcg aacagttcct
     5701 gattaaccac aaaccgttct actttactgg ctttggtcgt catgaagatg cggacttgcg
     5761 tggcaaagga ttcgataacg tgctgatggt gcacgaccac gcattaatgg actggattgg
     5821 ggccaactcc taccgtacct cgcattaccc ttacgctgaa gagatgctcg actgggcaga
     5881 tgaacatggc atcgtggtga ttgatgaaac tgctgctgtc ggctttaacc tctctttagg
     5941 cattggtttc gaagcgggca acaagccgaa agaactgtac agcgaagagg cagtcaacgg
     6001 ggaaactcag caagcgcact tacaggcgat taaagagctg atagcgcgtg acaaaaacca
     6061 cccaagcgtg gtgatgtgga gtattgccaa cgaaccggat acccgtccgc aaggtgcacg
     6121 ggaatatttc gcgccactgg cggaagcaac gcgtaaactc gacccgacgc gtccgatcac
     6181 ctgcgtcaat gtaatgttct gcgacgctca caccgatacc atcagcgatc tctttgatgt
     6241 gctgtgcctg aaccgttatt acggatggta tgtccaaagc ggcgatttgg aaacggcaga
     6301 gaaggtactg gaaaaagaac ttctggcctg gcaggagaaa ctgcatcagc cgattatcat
     6361 caccgaatac ggcgtggata cgttagccgg gctgcactca atgtacaccg acatgtggag
     6421 tgaagagtat cagtgtgcat ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc
     6481 cgtcgtcggt gaacaggtat ggaatttcgc cgattttgcg acctcgcaag gcatattgcg
     6541 cgttggcggt aacaagaaag ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt
     6601 tctgctgcaa aaacgctgga ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa
     6661 acaatgaata tgaagatgaa gatgaatatt tggtgtgtca aataaaaagc ttgtgtgctt
     6721 aagtttgtgt ttttttcttg gcttgttgtg ttatgaattt gtggcttttt ctaatattaa
     6781 atgaatgtaa gatctcatta taatgaataa acaaatgttt ctataatcca ttgtgaatgt
     6841 tttgttggat ctcttctgca gcatataact actgtatgtg ctatggtatg gactatggaa
     6901 tatgattaaa gataagatgg gctcatagag taaaacgagg cgagggacct ataaacctca
     6961 cttcatcatg ctatttcatg atctatttta taaaataaag atgtagaaaa aagtaagcgt
     7021 aataaccgca aaacaaatga tttaaaacat ggcacataat gaggagatta agttcggttt
     7081 acgtttattt tagtactaat tgtaacgtga gactacgtat cgggaatcgc ctaccgggta
     7141 ccggctggat cttatgacga ccgttagaaa ttgtggttgt cgacgagtca gtaataaacg
     7201 gcgtcaaagt ggttgcagcc ggcacacacg agtcgtgttt atcaactcaa agcacaaata
     7261 cttttcctca acctaaaaat aaggcaatta gccaaaaaca actttgcgtg taaacaacgc
     7321 tcaatacacg tgtcatttta ttattagcta ttgcttcacc gccttagctt tctcgtgacc
     7381 tagtcgtcct cgtcttttct tcttcttctt ctataaaaca atacccaaag agctcttctt
     7441 cttcacaatt cagatttcaa tttctcaaaa tcttaaaaac tttctctcaa ttctctctac
     7501 cgtgatcaag gtaaatttct gtgttcctta ttctctcaaa atcttcgatt ttgttttcgt
     7561 tcgatcccaa tttcgtatat gttctttggt ttagattctg ttaatcttag atcgaagacg
     7621 attttctggg tttgatcgtt agatatcatc ttaattctcg attagggttt catagatatc
     7681 atccgatttg ttcaaataat ttgagttttg tctaataatt actcttcgat ttgtgatttc
     7741 tatctagatc tggtgttagt ttctagtttg tgcgatcgaa tttgtcgatt aatctgagtt
     7801 tttctgatta acagatggcg aaggcagata agaaaccagc ggagaagaaa ccggcagaga
     7861 aaactccggc agtcgaacca gcagcagcgg cagagaagaa accaaaagcc ggaaagaaac
     7921 tccccaagga accagccgtc gccggagaca agaagaagaa gagatcaaag aagaacgttg
     7981 agacatacaa gatctacatc ttcaaggtgt tgaagcaagt tcatccagac atcggaatct
     8041 caagcaaagc catgggaatc atgaacagtt tcatcaacga tatctttgag aagcttgctg
     8101 gtgagtcttc gaagcttgcg aggtacaaca agaagccgac gattacttcc agggagattc
     8161 agactgcggt tagacttgtg ttgcccggtg agttggcgaa acatgctgtt tctgaaggga
     8221 ctaaggcggt tacgaagttt acgagttctg ctggagctgg agaattcatg gtgagcaagg
     8281 gcgaggcagt gatcaaggag ttcatgcggt tcaaggtgca catggagggc tccatgaacg
     8341 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     8401 ccaagctgaa ggtgaccaag ggtggccccc tgcccttctc ctgggacatc ctgtcccctc
     8461 agttcatgta cggctccagg gccttcacca agcaccccgc cgacatcccc gactactata
     8521 agcagtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     8581 ccgtgaccgt gacccaggac acctccctgg aggacggcac cctgatctac aaggtgaagc
     8641 tccgcggcac caacttccct cctgacggcc ccgtaatgca gaagaagaca atgggctggg
     8701 aagcgtccac cgagcggttg taccccgagg acggcgtgct gaagggcgac attaagatgg
     8761 ccctgcgcct gaaggacggc ggccgctacc tggcggactt caagaccacc tacaaggcca
     8821 agaagcccgt gcagatgccc ggcgcctaca acgtcgaccg caagttggac atcacctccc
     8881 acaacgagga ctacaccgtg gtggaacagt acgaacgctc cgagggccgc cactccaccg
     8941 gcggcatgga cgagctgtac aagtaggctt tttgtgatct gatgataagt ggttggttcg
     9001 tgtctcatgc acttgggagg tgatctattt cacctggtgt agtttgtgtt tccgtcagtt
     9061 ggaaaaactt atccctatcg atttcgtttt cattttctgc ttttctttta tgtaccttcg
     9121 tttgggcttg taacgggcct ttgtatttca actctcaata ataatccaag tgcatgttaa
     9181 acaatttgtc atctgtttcg gctttgatat actactggtg aagatgggcc gtactactgc
     9241 atcacaacga aaaataataa taagatgaaa aacttgaagt ggaaaaaaaa aaaaacttga
     9301 atgttcacta ctactcattg accataatgt ttaacataca tagctcaata gtatttttgt
     9361 gaatatggca acacaaacag tccaaaacaa ttgtctctta ctataccaaa ccaagggcgc
     9421 cgcttgtttg ccactctttg tgtgcaatag tgtgattacc acatctccac attcaatata
     9481 ttccctgaat tatctgacga ttttgatggc tcactgtttt cccaagtctg gtaccccacc
     9541 tacatggcag ctcgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     9601 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9661 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9721 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9781 tggctagagc agcttgccaa catggtggag cacgacactc tcgtctactc caagaatatc
     9841 aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg
     9901 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa
     9961 aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
    10021 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
    10081 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgaacatgg tggagcacga
    10141 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
    10201 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
    10261 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
    10321 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc
    10381 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
    10441 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
    10501 cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa atcaccagtc
    10561 tctctctaca aatctatctc tctcgagctt tcgcagatct gtcgatcgac catggggatt
    10621 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
    10681 gactgggcac aacagacaat cggctgctct gatgccgccg tgtttcggct gtcagcgcag
    10741 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actccaggac
    10801 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
    10861 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
    10921 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
    10981 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
    11041 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
    11101 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
    11161 gatctcgtcg tgacacatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
    11221 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
    11281 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
    11341 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
    11401 ttcttctgag cgggactctg gggttcggat cgatcctcta gctagagtcg atcgacaagc
    11461 tcgagtttct ccataataat gtgtgagtag ttcccagata agggaattag ggttcctata
    11521 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
    11581 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc
    11641 ccccgaatta attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt
    11701 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
    11761 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
    11821 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
    11881 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
    11941 gttgattgta acgatgacag agcgttgctg cctgtgatca ccgcggtttc aaaatcggct
    12001 ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt
    12061 atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt
    12121 cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag
    12181 aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg
    12241 aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat
    12301 gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct
    12361 atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg
    12421 gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga
    12481 acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat
    12541 cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta
    12601 cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt
    12661 taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt
    12721 cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg
    12781 ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt
    12841 ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact
    12901 ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta
    12961 gtacctagaa tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    13021 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    13081 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    13141 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
    13201 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    13261 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    13321 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    13381 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    13441 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    13501 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    13561 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    13621 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    13681 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    13741 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    13801 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
    13861 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
    13921 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
    13981 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
    14041 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
    14101 cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc
    14161 tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg
    14221 acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc
    14281 ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat
    14341 aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta
    14401 catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc
    14461 aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc
    14521 tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga
    14581 tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc
    14641 ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa
    14701 acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca
    14761 tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc
    14821 gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt
    14881 gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc
    14941 gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg
    15001 gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca
    15061 ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac
    15121 gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg
    15181 gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact
    15241 ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat
    15301 cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct
    15361 gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc
    15421 gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt
    15481 gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg
    15541 ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc
    15601 gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg
    15661 gttccagtgc cattgcaggg ccggcaggca acccagccgc ttacgcctgg ccaaccgccc
    15721 gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg
    15781 ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg
    15841 tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta
    15901 catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg
    15961 cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc
    16021 acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt
    16081 gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat
    16141 tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg
    16201 gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc
    16261 gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca
    16321 atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc
    16381 tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc
    16441 tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca
    16501 atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa
    16561 tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc
    16621 agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt
    16681 acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat
    16741 acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc
    16801 ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca
    16861 aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt
    16921 acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc
    16981 cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc
    17041 gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt
    17101 cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc
    17161 agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg
    17221 tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg
    17281 cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac
    17341 ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg
    17401 caggcccgcg ccggcctcct ggatgcg
//
