LOCUS       sequence-319135-       17367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PP7-tagged reporter driven by the Arabidopsis HSP70 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17367)
  AUTHORS   Alamos S, Reimer A, Niyogi KK, Garcia HG
  TITLE     Quantitative imaging of RNA polymerase II activity in plants reveals
            the single-cell basis of tissue-wide transcriptional dynamics.
  JOURNAL   Nat Plants. 2021 Aug;7(8):1037-1049. doi: 
            10.1038/s41477-021-00976-0. Epub 2021 Aug 9.
  PUBMED    34373604
REFERENCE   2  (bases 1 to 17367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-00976-0"; journalName: "Nat Plants"; date: 
            "2021-08"; volume: "7"; issue: "8"; pages: "1037-1049"
COMMENT     Sequence Label: AL12R-HSP70
FEATURES             Location/Qualifiers
     source          1..17367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    571..595
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(1190..1885)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(1465..1484)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     intron          7397..7585
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     terminator      9014..9261
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(9508..9524)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9508..9524)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9521..9543)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9532..9548
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9556..9562,9563..9580,9581..9586))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9601..9622
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9813..10489
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10426..10446
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             10552..11349
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10609..10628)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11219..11238
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11406..11580
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11658..11682
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12107..12901
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12988..13576
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13477..13496
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13730..13747
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    13762..13902
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(13828..13847)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13988..14010
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      14246..14440
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14506..15579)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(16008..16637)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
       61 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
      121 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
      181 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
      241 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
      301 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
      361 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
      421 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
      481 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
      541 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
      601 tgataccatg taggtggggt accagacttg ggaaaacagt gagccatcaa aatcgtcaga
      661 taattcaggg aatatattga atgtggagat gtggtaatca cactattgca cacaaagagt
      721 ggcaaacaag cggcgccctt ggtttggtat agtaagagac aattgttttg gactgtttgt
      781 gttgccatat tcacaaaaat actattgagc tatgtatgtt aaacattatg gtcaatgagt
      841 agtagtgaac attcaagttt tttttttttt ccacttcaag tttttcatct tattattatt
      901 tttcgttgtg atgcagtagt acggcccatc ttcaccagta gtatatcaaa gccgaaacag
      961 atgacaaatt gtttaacatg cacttggatt attattgaga gttgaaatac aaaggcccgt
     1021 tacaagccca aacgaaggta cataaaagaa aagcagaaaa tgaaaacgaa atcgataggg
     1081 ataagttttt ccaactgacg gaaacacaaa ctacaccagg tgaaatagat cacctcccaa
     1141 gtgcatgaga cacgaaccaa ccacttatca tcagatcaca aaaagcctac ttgtacagct
     1201 cgtccatgcc gccggtggag tggcggccct cggagcgttc gtactgttcc accacggtgt
     1261 agtcctcgtt gtgggaggtg atgtccaact tgcggtcgac gttgtaggcg ccgggcatct
     1321 gcacgggctt cttggccttg taggtggtct tgaagtccgc caggtagcgg ccgccgtcct
     1381 tcaggcgcag ggccatctta atgtcgccct tcagcacgcc gtcctcgggg tacaaccgct
     1441 cggtggacgc ttcccagccc attgtcttct tctgcattac ggggccgtca ggagggaagt
     1501 tggtgccgcg gagcttcacc ttgtagatca gggtgccgtc ctccagggag gtgtcctggg
     1561 tcacggtcac ggcgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
     1621 ggaaggactg cttatagtag tcggggatgt cggcggggtg cttggtgaag gccctggagc
     1681 cgtacatgaa ctgaggggac aggatgtccc aggagaaggg cagggggcca cccttggtca
     1741 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
     1801 cgaactcgtg gccgttcatg gagccctcca tgtgcacctt gaaccgcatg aactccttga
     1861 tcactgcctc gcccttgctc accatgaatt ctccagctcc agcagaactc gtaaacttcg
     1921 taaccgcctt agtcccttca gaaacagcat gtttcgccaa ctcaccgggc aacacaagtc
     1981 taaccgcagt ctgaatctcc ctggaagtaa tcgtcggctt cttgttgtac ctcgcaagct
     2041 tcgaagactc accagcaagc ttctcaaaga tatcgttgat gaaactgttc atgattccca
     2101 tggctttgct tgagattccg atgtctggat gaacttgctt caacaccttg aagatgtaga
     2161 tcttgtatgt ctcaacgttc ttctttgatc tcttcttctt cttgtctccg gcgacggctg
     2221 gttccttggg gagtttcttt ccggcttttg gtttcttctc tgccgctgct gctggttcga
     2281 ctgccggagt tttctctgcc ggtttcttct ccgctggttt cttatctgcc ttcgccatct
     2341 gttaatcaga aaaactcaga ttaatcgaca aattcgatcg cacaaactag aaactaacac
     2401 cagatctaga tagaaatcac aaatcgaaga gtaattattc gacaaaactc aaattatttg
     2461 aacaaatcgg atgatatcta tgaaacccta atcgagaatt aagatgatat ctaacgatca
     2521 aacccagaaa atcgtcttcg atctaagatt aacagaatct aaaccaaaga acatatacga
     2581 aattgggatc gaacgaaaac aaaatcgaag attttgagag aataaggaac acagaaattt
     2641 accttgatca cggtagagag aattgagaga aagtttttaa gattttgaga aattgaaatc
     2701 tgaattgtga agaagaagag ctctttgggt attgttttat agaagaagaa gaagaaaaga
     2761 cgaggacgac taggtcacga gaaagctaag gcggtgaagc aatagctaat aataaaatga
     2821 cacgtgtatt gagcgttgtt tacacgcaaa gttgtttttg gctaattgcc ttatttttag
     2881 gttgaggaaa agtatttgtg ctttgagttg ataaacacga ctcgtgtgtg ccggctgcaa
     2941 ccactttgac gccgtttatt actgactcgt cgacaaccac aatttctaac ggtcgtcata
     3001 agatccagcc ggtttaatct aaaggtcgtg ggttcgattc ccactgggat catcccattt
     3061 ttttggtctt tttttttgtt ggttattaat tattactatc aatccgttct tttagcagtg
     3121 atcaagcgaa tttaggtctg gctctgcctc tggctctagt tgagtttagg gagctgctcc
     3181 ttgttgtttg ccattatggt ttctctactt tggagtcaac tttgcaatgt tacccttgta
     3241 gtctcttctg tggactttgt atttggtgaa attgaaaagt ttagtagaaa tctggctgcg
     3301 ccaaagatga tagagactct aatgggatcc ttattcgaac attttgctga actgattaga
     3361 tacaataagg caacactgat tccattactg ttagtctgtt actacaaaaa gaattgttgc
     3421 tcaagtattg tttgtgttgg ttggtgcaga tgatcgatga tcaggacttg gggtttattg
     3481 ccaactttct tggcatcttc atcttcgcat tggtaattgc ttatcactac gtaactgctg
     3541 atcccaaata cgaagccact tgagtgatga tattttagaa tgatgtaagg ctttttagtt
     3601 tatactagta ttatctgtgt ttcaaactta gaagagataa taacagtctt tgttgagatg
     3661 ataatgtttt caagatgttc ctaatccatt tcacatcttc tcaattttat atgcatgtgc
     3721 atatatatgt tccctccaat tatgttgttc gaatgtttga tgaaactttg aatttttttc
     3781 tttaagcaaa aaaaaatctc aaacaccaaa gcgaggagtc attctagttc agttttgagt
     3841 cattctagtt atttttacaa agttttgagt caaattgggt aaattttttg gttattttgg
     3901 tcataaaaat aactagatta tctcttatat cttatgagtt aatttggtaa ataaaccatt
     3961 tatttgggtc aaactatttt tttccccata tatatatcca atcaataata aattcataat
     4021 atatttcatt aacgcgattg aaatactagt aattaattga ggactaaaga aaaagtaatt
     4081 tcctttttat ctttaaaatg tgcaaaaaaa acaaaaatgt taattgggtg atgaaataac
     4141 ttgttttcaa aacgggagtt actatttgac aatttaaaaa agacccatct cgaaggagct
     4201 agaagcgata acaaaataaa aaggaaacaa tagtaattag atggcgcaaa aataagatcc
     4261 aacggctgag atctttactc gtgaacgttc tcgaaagctc tttgccgacc cactcttcat
     4321 tcatatataa acaaacacct ctctgccttc tcttcctcac acaatcataa acacaacaac
     4381 actcacaaat tctcttaaag ctcacagacg aattctttct atttttaatc tttccggcga
     4441 acaattctga tctctaatag ggatcctacg gtacttattg ccaagaaagc acgagacgat
     4501 atggcgtccg tgcctccagg tcgaatcttc aaacgacgag aggatatggc ctccgtcgct
     4561 ccagtattcc agggttcatc agatcctacg gtacttattg ccaagaaagc acgagacgat
     4621 atggcgtccg tgcctccagg tcgaatcttc aaacgacgag aggatatggc ctccgtcgct
     4681 ccagtattcc agggttcatc agatcctacg gtacttattg ccaagaaagc acgagacgat
     4741 atggcgtccg tgcctccagg tcgaatcttc aaacgacgag aggatatggc ctccgtcgct
     4801 ccagtattcc agggttcatc agatcctacg gtacttattg ccaagaaagc acgagacgat
     4861 atggcgtccg tgcctccagg tcgaatcttc aaacgacgag aggatatggc ctccgtcgct
     4921 ccagtattcc agggttcatc agatcctacg gtacttattg ccaagaaagc acgagacgat
     4981 atggcgtccg tgcctccagg tcgaatcttc aaacgacgag aggatatggc ctccgtcgct
     5041 ccagtattcc agggttcatc agatcctacg gtacttattg ccaagaaagc acgagacgat
     5101 atggcgtccg tgcctccagg tcgaatcttc aaacgacgag aggatatggc ctccgtcgct
     5161 ccagtattcc agggttcatc agatcctacg gtacttattg ccaagaaagc acgagacgat
     5221 atggcgtccg tgcctccagg tcgaatcttc aaacgacgag aggatatggc ctccgtcgct
     5281 ccagtattcc agggttcatc agatcctacg gtacttattg ccaagaaagc acgagacgat
     5341 atggcgtccg tgcctccagg tcgaatcttc aaacgacgag aggatatggc ctccgtcgct
     5401 ccagtattcc agggttcatc agatcctacg gtacttattg ccaagaaagc acgagacgat
     5461 atggcgtccg tgcctccagg tcgaatcttc aaacgacgag aggatatggc ctccgtcgct
     5521 ccagtattcc agggttcatc agatcctacg gtacttattg ccaagaaagc acgagacgat
     5581 atggcgtccg tgcctccagg tcgaatcttc aaacgacgag aggatatggc ctccgtcgct
     5641 ccagtattcc agggttcatc agatcctacg gtacttattg ccaagaaagc acgagacgat
     5701 atggcgtccg tgcctccagg tcgaatcttc aaacgacgag aggatatggc ctccgtcgct
     5761 ccagtattcc agggttcatc agatcctacg gtacttattg ccaagaaagc acgagacgat
     5821 atggcgtccg tgcctccagg tcgaatcttc aaacgacgag aggatatggc ctccgtcgct
     5881 ccagtattcc agggttcatc agagtgccag agtccttcga tagggacaag acaattgcac
     5941 tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg cctcatagaa
     6001 ctgcctgcgt gagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg
     6061 atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg
     6121 gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttc
     6181 tgaggagcct tcaggattac aagattcaaa gtgcgctgct ggtgccaacc ctattctcct
     6241 tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt
     6301 ctggtggcgc tcccctctct aaggaagtcg gggaagcggt tgccaagagg ttccatctgc
     6361 caggtatcag gcaaggatat gggctcactg agactacatc agctattctg attacacccg
     6421 agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg
     6481 tggatctgga taccgggaaa acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag
     6541 gtcctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca
     6601 aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca
     6661 tcgttgaccg cctgaagtct ctgattaagt acaaaggcta tcaggtggct cccgctgaat
     6721 tggaatccat cttgctccaa caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg
     6781 acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga
     6841 cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg
     6901 gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa
     6961 aaatcagaga gatcctcata aaggccaaga agggcggaaa gatcgccgtg tatggtccgt
     7021 cctgtagaaa ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg
     7081 gatcgcgaaa actgtggaat tggtcagcgt tggtgggaaa gcgcgttaca agaaagccgg
     7141 gcaattgctg tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat
     7201 gcgggcaacg tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt
     7261 atcgtgctgc gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa
     7321 gtgatggagc atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt
     7381 gccgggaaaa gtctacgtaa gtttctgctt ctacctttga tatatatata ataattatca
     7441 ttaattagta gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat
     7501 agcaattgct tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat
     7561 atgaccaaaa tttgttgatg tgcaggtatc accgtttgtg tgaacaacga actgaactgg
     7621 cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa gcagtcttac
     7681 ttccatgatt tctttaacta tgccggaatc catcgcagcg taatgctcta caccacgccg
     7741 aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg taaccacgcg
     7801 tctgttgact ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat
     7861 caacaggtgg ttgcaactgg acaaggcact agcgggactt tgcaagtggt gaatccgcac
     7921 ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa aagccagaca
     7981 gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa gggcgaacag
     8041 ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga agatgcggac
     8101 ttgcgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt aatggactgg
     8161 attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat gctcgactgg
     8221 gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt taacctctct
     8281 ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga agaggcagtc
     8341 aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc gcgtgacaaa
     8401 aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg tccgcaaggt
     8461 gcacgggaat atttcgcgcc actggcggaa gcaacgcgta aactcgaccc gacgcgtccg
     8521 atcacctgcg tcaatgtaat gttctgcgac gctcacaccg ataccatcag cgatctcttt
     8581 gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc aaagcggcga tttggaaacg
     8641 gcagagaagg tactggaaaa agaacttctg gcctggcagg agaaactgca tcagccgatt
     8701 atcatcaccg aatacggcgt ggatacgtta gccgggctgc actcaatgta caccgacatg
     8761 tggagtgaag agtatcagtg tgcatggctg gatatgtatc accgcgtctt tgatcgcgtc
     8821 agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc gcaaggcata
     8881 ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc gaagtcggcg
     8941 gcttttctgc tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc gcagcaggga
     9001 ggcaaacaat gaatatgaag atgaagatga atatttggtg tgtcaaataa aaagcttgtg
     9061 tgcttaagtt tgtgtttttt tcttggcttg ttgtgttatg aatttgtggc tttttctaat
     9121 attaaatgaa tgtaagatct cattataatg aataaacaaa tgtttctata atccattgtg
     9181 aatgttttgt tggatctctt ctgcagcata taactactgt atgtgctatg gtatggacta
     9241 tggaatatga ttaaagataa gatgggctca tagagtaaaa cgaggcgagg gacctataaa
     9301 cctcacttca tcatgctatt tcatgatcta ttttataaaa taaagatgta gaaaaaagta
     9361 agcgtaataa ccgcaaaaca aatgatttaa aacatggcac ataatgagga gattaagttc
     9421 ggtttacgtt tattttagta ctaattgtaa cgtgagacta cgtatcggga atcgcctacc
     9481 gggtaccgag ctcgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     9541 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9601 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9661 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9721 tggctagagc agcttgccaa catggtggag cacgacactc tcgtctactc caagaatatc
     9781 aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg
     9841 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa
     9901 aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
     9961 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
    10021 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgaacatgg tggagcacga
    10081 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
    10141 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
    10201 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
    10261 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc
    10321 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
    10381 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
    10441 cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa atcaccagtc
    10501 tctctctaca aatctatctc tctcgagctt tcgcagatct gtcgatcgac catggggatt
    10561 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
    10621 gactgggcac aacagacaat cggctgctct gatgccgccg tgtttcggct gtcagcgcag
    10681 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actccaggac
    10741 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
    10801 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
    10861 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
    10921 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
    10981 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
    11041 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
    11101 gatctcgtcg tgacacatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
    11161 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
    11221 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
    11281 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
    11341 ttcttctgag cgggactctg gggttcggat cgatcctcta gctagagtcg atcgacaagc
    11401 tcgagtttct ccataataat gtgtgagtag ttcccagata agggaattag ggttcctata
    11461 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
    11521 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc
    11581 ccccgaatta attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt
    11641 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
    11701 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
    11761 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
    11821 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
    11881 gttgattgta acgatgacag agcgttgctg cctgtgatca ccgcggtttc aaaatcggct
    11941 ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt
    12001 atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt
    12061 cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag
    12121 aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg
    12181 aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat
    12241 gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct
    12301 atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg
    12361 gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga
    12421 acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat
    12481 cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta
    12541 cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt
    12601 taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt
    12661 cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg
    12721 ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt
    12781 ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact
    12841 ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta
    12901 gtacctagaa tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    12961 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    13021 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    13081 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
    13141 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    13201 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    13261 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    13321 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    13381 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    13441 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    13501 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    13561 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    13621 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    13681 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    13741 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
    13801 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
    13861 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
    13921 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
    13981 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
    14041 cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc
    14101 tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg
    14161 acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc
    14221 ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat
    14281 aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta
    14341 catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc
    14401 aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc
    14461 tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga
    14521 tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc
    14581 ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa
    14641 acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca
    14701 tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc
    14761 gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt
    14821 gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc
    14881 gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg
    14941 gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca
    15001 ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac
    15061 gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg
    15121 gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact
    15181 ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat
    15241 cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct
    15301 gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc
    15361 gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt
    15421 gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg
    15481 ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc
    15541 gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg
    15601 gttccagtgc cattgcaggg ccggcaggca acccagccgc ttacgcctgg ccaaccgccc
    15661 gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg
    15721 ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg
    15781 tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta
    15841 catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg
    15901 cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc
    15961 acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt
    16021 gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat
    16081 tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg
    16141 gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc
    16201 gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca
    16261 atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc
    16321 tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc
    16381 tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca
    16441 atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa
    16501 tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc
    16561 agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt
    16621 acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat
    16681 acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc
    16741 ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca
    16801 aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt
    16861 acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc
    16921 cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc
    16981 gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt
    17041 cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc
    17101 agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg
    17161 tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg
    17221 cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac
    17281 ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg
    17341 caggcccgcg ccggcctcct ggatgcg
//
