LOCUS       sequence-319137-       12226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ubiquitously weakly expressed 60-meric GFP nanocage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Alamos S, Reimer A, Niyogi KK, Garcia HG
  TITLE     Quantitative imaging of RNA polymerase II activity in plants reveals
            the single-cell basis of tissue-wide transcriptional dynamics.
  JOURNAL   Nat Plants. 2021 Aug;7(8):1037-1049. doi: 
            10.1038/s41477-021-00976-0. Epub 2021 Aug 9.
  PUBMED    34373604
REFERENCE   2  (bases 1 to 12226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-00976-0"; journalName: "Nat Plants"; date: 
            "2021-08"; volume: "7"; issue: "8"; pages: "1037-1049"
COMMENT     Sequence Label: UBC1cer60G
FEATURES             Location/Qualifiers
     source          1..12226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    384..408
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     596..618
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     610..627
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     611..627
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2606..3316
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(2633..2661)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3256..3276
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(3964..3980)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3964..3980)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3977..3999)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3988..4004
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4012..4018,4019..4036,4037..4042))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4057..4078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4269..4945
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     4882..4902
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             5012..6037
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    6078..6252
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6330..6354
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6779..7573
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7660..8248
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8149..8168
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8402..8419
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8434..8574
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8500..8519)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8660..8682
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8918..9112
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9178..10251)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10680..11309)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc
       61 ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa
      121 cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac
      181 ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta
      241 tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat
      301 gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc
      361 taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt
      421 ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca
      481 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
      541 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
      601 tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt cgactctaga
      661 ggatccgttt tgctgatagg tttcgtcagg attgatatat gttcgtaggt agcagaagag
      721 actaaaagta gtttattttt tcgtcctttt gaccaaatgt tcaaaagcca catatcattt
      781 cgtggtaagc ggttgtatct caaaacatca gtctgatctt agttgttttt gatttaagag
      841 caaatctcaa tttgaagtct gagtacgagc tagtatgatc caaatgccta ggctttgaga
      901 cgaggcttga agatgaattg gtttgtctag agagattgga gaaacaagac ataccacaaa
      961 aacatttctc tctagtaaca aaaacatcac tctactctac tcttgtggtt tgggtctgga
     1021 ttttagtccg gttagtggta caatggttta tattggttta ttagaaatat atagtttacg
     1081 ctacattaag aaaagtactc taatttgtca tctgaagatt tacataaaaa agattacatg
     1141 tcatcgttgt atgttaacta acactaatct cccggttaaa gtgtagattg ccctaaacgg
     1201 tttaattccg gttaggaaca aaccaaccac tcgctcggtc gtccaaaggg caaagcgaat
     1261 ccaactttac agatatgtca gagaggagcc aagcttctag aagatcattc attcatcact
     1321 tatacgatct cttcccaaat tattgatttt ttttacccat ttctctcatc gcacggtgat
     1381 attgagaatc gccgacctga atcgatcgga aaactttctc tgattaccgg cggtcaacac
     1441 cgctgaacac atatgtttgt ttgacgacct cttctctccg cgatctttac ctcaacaacg
     1501 agatctgttt ccacggtacc attttcatcc gatctctgtt cttctatgtt tgtttactgg
     1561 atccgttgat tttactttca tcttatcatt tgtgtgtggc tattttggtg aattgatgcg
     1621 atcttaagct gtttctaggt tcaagcctta tatgtttctt ctaagctaat cataggtcgt
     1681 agacttgtta ggaagattcg aatctagtac aaagttagca actttagcat acgtaaatcg
     1741 ttagtagtaa tttctagatt gatagaggtt cgagacctta aaaggtgatg aatatttggt
     1801 gggttttgtt tattagaaag aaaggaggat gatggatccg gtagtagtgc tgggtttgtg
     1861 tttgagctgt ctcctgttgc tgagtctctg gaagcaatcc tacggcggtg gcaaactggg
     1921 cggaggtggc ggtggagcta gcatgaaaat ggaggaactc tttaaaaagc acaagatcgt
     1981 ggcagtactc cgggccaata gcgtagagga ggctaagaaa aaagctctcg ctgtgtttct
     2041 gggcggcgtg cacttgatcg agataacttt tacggttcct gacgccgata cggtcatcaa
     2101 agaactgagt tttttgaaag aaatgggagc aataatcgga gccggcacag ttacgtccgt
     2161 ggaacagtgt cggaaagcag ttgagtcggg cgcggagttt atcgttagtc cgcatctcga
     2221 cgaggagatc agccaattct gcaaagagaa aggagtattt tacatgcccg gcgtgatgac
     2281 accgactgag ctggtcaaag ctatgaagct gggccacact atactcgccc tgtttccagg
     2341 agaagttgtt ggccctcaat tcgtaaaagc gatgaagggt cccttcccca acgtaaaatt
     2401 cgttccaacg ggcggagtca acctggataa cgtatgcgag tggtttaaag ccggcgtttt
     2461 ggctgttggt gtaggaagtg ctctcgttaa aggtacgcct gtcgaagtgg ctgagaaagc
     2521 taaagccttt gttgaaaaga tacgtggctg taccgaagga ggcagcggcg gatcaggagg
     2581 gtcaggtgga tcagtagatc tgactagtaa aggagaagaa cttttcactg gagttgtccc
     2641 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
     2701 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
     2761 acctgttccg tggccaacac ttgtcactac tttctcttat ggtgttcaat gcttttcaag
     2821 atacccagat catatgaagc ggcacgactt cttcaagagc gccatgcctg agggatacgt
     2881 gcaggagagg accatcttct tcaaggacga cgggaactac aagacacgtg ctgaagtcaa
     2941 gtttgaggga gacaccctcg tcaacaggat cgagcttaag ggaatcgatt tcaaggagga
     3001 cggaaacatc ctcggccaca agttggaata caactacaac tcccacaacg tatacatcat
     3061 ggccgacaag caaaagaacg gcatcaaagc caacttcaag acccgccaca acatcgaaga
     3121 cggcggcgtg caactcgctg atcattatca acaaaatact ccaattggcg atggccctgt
     3181 ccttttacca gacaaccatt acctgtccac acaatctaag ctttcgaaag atcccaacga
     3241 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
     3301 ggatgaacta tacaaagcta gcccatggta agctttttgt gatctgatga taagtggttg
     3361 gttcgtgtct catgcacttg ggaggtgatc tatttcacct ggtgtagttt gtgtttccgt
     3421 cagttggaaa aacttatccc tatcgatttc gttttcattt tctgcttttc ttttatgtac
     3481 cttcgtttgg gcttgtaacg ggcctttgta tttcaactct caataataat ccaagtgcat
     3541 gttaaacaat ttgtcatctg tttcggcttt gatatactac tggtgaagat gggccgtact
     3601 actgcatcac aacgaaaaat aataataaga tgaaaaactt gaagtggaaa aaaaaaaaac
     3661 ttgaatgttc actactactc attgaccata atgtttaaca tacatagctc aatagtattt
     3721 ttgtgaatat ggcaacacaa acagtccaaa acaattgtct cttactatac caaaccaagg
     3781 gcgccgcttg tttgccactc tttgtgtgca atagtgtgat taccacatct ccacattcaa
     3841 tatattccct gaattatctg acgattttga tggctcactg ttttcccaag tctggtaccc
     3901 cacctacatg gggatcctaa ctcgagctgc aggcttcggt accgagctcg aattcgtaat
     3961 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     4021 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     4081 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     4141 aatcggccaa cgcgcgggga gaggcggttt gcgtattggc tagagcagct tgccaacatg
     4201 gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa
     4261 agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc
     4321 ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc
     4381 catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctgccgacag tggtcccaaa
     4441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     4501 aagcaagtgg attgatgtga acatggtgga gcacgacact ctcgtctact ccaagaatat
     4561 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc
     4621 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga
     4681 aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga
     4741 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
     4801 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt
     4861 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
     4921 atttcatttg gagaggacac gctgaaatca ccagtctctc tctacaaatc tatctctctc
     4981 gagctttcgc agatccgggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc
     5041 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     5101 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     5161 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     5221 cgcgctcccg attccggaag tgcttgacat tggggagttt agcgagagcc tgacctattg
     5281 catctcccgc cgttcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     5341 tgttctacaa ccggtcgcgg aggctatgga tgcgatcgct gcggccgatc ttagccagac
     5401 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     5461 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     5521 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     5581 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     5641 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     5701 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     5761 gcggaggcat ccggagcttg caggatcgcc acgactccgg gcgtatatgc tccgcattgg
     5821 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     5881 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     5941 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     6001 ccgacgcccc agcactcgtc cgagggcaaa gaaatagagt agatgccgac cgggatctgt
     6061 cgatcgacaa gctcgagttt ctccataata atgtgtgagt agttcccaga taagggaatt
     6121 agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt
     6181 gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtact
     6241 aaaatccaga tcccccgaat taattcggcg ttaattcagt acattaaaaa cgtccgcaat
     6301 gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc
     6361 agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca
     6421 tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta
     6481 ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc
     6541 ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat caccgcggtt
     6601 tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaacaac tttgaaaaag
     6661 ctgttttctg gtatttaagg ttttagaatg caaggaacag tgaattggag ttcgtcttgt
     6721 tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga aataataaat
     6781 ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa
     6841 agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa atgaaaacct
     6901 atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg aacgggaaaa
     6961 ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc actttgaacg
     7021 gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt gctcggaaga
     7081 gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt gcatcaggct
     7141 ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca gccgcttagc
     7201 cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa actgggaaga
     7261 agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga
     7321 agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg tgaaagatgg
     7381 caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt ggtatgacat
     7441 tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg tcgagctatt
     7501 ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata ttttactgga
     7561 tgaattgttt tagtacctag aatgcatgac caaaatccct taacgtgagt tttcgttcca
     7621 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7681 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7741 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7801 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7861 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7921 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7981 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8041 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8101 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     8161 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8221 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8281 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     8341 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     8401 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     8461 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     8521 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     8581 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     8641 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     8701 aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc
     8761 ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc
     8821 cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc
     8881 tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt
     8941 aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat
     9001 atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg
     9061 gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag
     9121 accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta
     9181 ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc
     9241 agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt
     9301 gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt
     9361 cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa
     9421 acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt
     9481 cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg
     9541 ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac
     9601 cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt
     9661 taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca
     9721 gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc
     9781 ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta
     9841 atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag
     9901 ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac
     9961 gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc
    10021 tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg
    10081 ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc
    10141 tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc
    10201 acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc
    10261 tcgggcttgg gggttccagt gccattgcag ggccggcagg caacccagcc gcttacgcct
    10321 ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct
    10381 tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct
    10441 catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg
    10501 gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg
    10561 cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct
    10621 cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact
    10681 caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc
    10741 tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc
    10801 tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca
    10861 ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca
    10921 tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg
    10981 taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc
    11041 aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc
    11101 acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc
    11161 acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc
    11221 ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct
    11281 ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc
    11341 atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc
    11401 tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct
    11461 tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg
    11521 gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg
    11581 gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc
    11641 ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc
    11701 ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac
    11761 gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc
    11821 gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt
    11881 ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat
    11941 gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc
    12001 tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc
    12061 gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag
    12121 gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga
    12181 aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttg
//
