LOCUS       sequence-319141-       16190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PP7-tagged reporter without a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16190)
  AUTHORS   Alamos S, Reimer A, Niyogi KK, Garcia HG
  TITLE     Quantitative imaging of RNA polymerase II activity in plants reveals
            the single-cell basis of tissue-wide transcriptional dynamics.
  JOURNAL   Nat Plants. 2021 Aug;7(8):1037-1049. doi: 
            10.1038/s41477-021-00976-0. Epub 2021 Aug 9.
  PUBMED    34373604
REFERENCE   2  (bases 1 to 16190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-00976-0"; journalName: "Nat Plants"; date: 
            "2021-08"; volume: "7"; issue: "8"; pages: "1037-1049"
COMMENT     Sequence Label: AL12R
FEATURES             Location/Qualifiers
     source          1..16190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    571..595
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(1190..1885)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(1465..1484)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3189..3211
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3203..3220
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3204..3220
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          6220..6408
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     terminator      7837..8084
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(8331..8347)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8331..8347)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8344..8366)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8355..8371
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8379..8385,8386..8403,8404..8409))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8424..8445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8636..9312
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     9249..9269
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             9375..10172
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9432..9451)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10042..10061
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10229..10403
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    10481..10505
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             10930..11724
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11811..12399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12300..12319
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12553..12570
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12585..12725
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12651..12670)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12811..12833
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      13069..13263
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(13329..14402)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(14831..15460)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
       61 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
      121 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
      181 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
      241 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
      301 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
      361 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
      421 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
      481 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
      541 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
      601 tgataccatg taggtggggt accagacttg ggaaaacagt gagccatcaa aatcgtcaga
      661 taattcaggg aatatattga atgtggagat gtggtaatca cactattgca cacaaagagt
      721 ggcaaacaag cggcgccctt ggtttggtat agtaagagac aattgttttg gactgtttgt
      781 gttgccatat tcacaaaaat actattgagc tatgtatgtt aaacattatg gtcaatgagt
      841 agtagtgaac attcaagttt tttttttttt ccacttcaag tttttcatct tattattatt
      901 tttcgttgtg atgcagtagt acggcccatc ttcaccagta gtatatcaaa gccgaaacag
      961 atgacaaatt gtttaacatg cacttggatt attattgaga gttgaaatac aaaggcccgt
     1021 tacaagccca aacgaaggta cataaaagaa aagcagaaaa tgaaaacgaa atcgataggg
     1081 ataagttttt ccaactgacg gaaacacaaa ctacaccagg tgaaatagat cacctcccaa
     1141 gtgcatgaga cacgaaccaa ccacttatca tcagatcaca aaaagcctac ttgtacagct
     1201 cgtccatgcc gccggtggag tggcggccct cggagcgttc gtactgttcc accacggtgt
     1261 agtcctcgtt gtgggaggtg atgtccaact tgcggtcgac gttgtaggcg ccgggcatct
     1321 gcacgggctt cttggccttg taggtggtct tgaagtccgc caggtagcgg ccgccgtcct
     1381 tcaggcgcag ggccatctta atgtcgccct tcagcacgcc gtcctcgggg tacaaccgct
     1441 cggtggacgc ttcccagccc attgtcttct tctgcattac ggggccgtca ggagggaagt
     1501 tggtgccgcg gagcttcacc ttgtagatca gggtgccgtc ctccagggag gtgtcctggg
     1561 tcacggtcac ggcgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
     1621 ggaaggactg cttatagtag tcggggatgt cggcggggtg cttggtgaag gccctggagc
     1681 cgtacatgaa ctgaggggac aggatgtccc aggagaaggg cagggggcca cccttggtca
     1741 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
     1801 cgaactcgtg gccgttcatg gagccctcca tgtgcacctt gaaccgcatg aactccttga
     1861 tcactgcctc gcccttgctc accatgaatt ctccagctcc agcagaactc gtaaacttcg
     1921 taaccgcctt agtcccttca gaaacagcat gtttcgccaa ctcaccgggc aacacaagtc
     1981 taaccgcagt ctgaatctcc ctggaagtaa tcgtcggctt cttgttgtac ctcgcaagct
     2041 tcgaagactc accagcaagc ttctcaaaga tatcgttgat gaaactgttc atgattccca
     2101 tggctttgct tgagattccg atgtctggat gaacttgctt caacaccttg aagatgtaga
     2161 tcttgtatgt ctcaacgttc ttctttgatc tcttcttctt cttgtctccg gcgacggctg
     2221 gttccttggg gagtttcttt ccggcttttg gtttcttctc tgccgctgct gctggttcga
     2281 ctgccggagt tttctctgcc ggtttcttct ccgctggttt cttatctgcc ttcgccatct
     2341 gttaatcaga aaaactcaga ttaatcgaca aattcgatcg cacaaactag aaactaacac
     2401 cagatctaga tagaaatcac aaatcgaaga gtaattattc gacaaaactc aaattatttg
     2461 aacaaatcgg atgatatcta tgaaacccta atcgagaatt aagatgatat ctaacgatca
     2521 aacccagaaa atcgtcttcg atctaagatt aacagaatct aaaccaaaga acatatacga
     2581 aattgggatc gaacgaaaac aaaatcgaag attttgagag aataaggaac acagaaattt
     2641 accttgatca cggtagagag aattgagaga aagtttttaa gattttgaga aattgaaatc
     2701 tgaattgtga agaagaagag ctctttgggt attgttttat agaagaagaa gaagaaaaga
     2761 cgaggacgac taggtcacga gaaagctaag gcggtgaagc aatagctaat aataaaatga
     2821 cacgtgtatt gagcgttgtt tacacgcaaa gttgtttttg gctaattgcc ttatttttag
     2881 gttgaggaaa agtatttgtg ctttgagttg ataaacacga ctcgtgtgtg ccggctgcaa
     2941 ccactttgac gccgtttatt actgactcgt cgacaaccac aatttctaac ggtcgtcata
     3001 agatccagcc ggtttaaact gaaggcggga aacgacaatc tgatccaagc tcaagctgct
     3061 ctagcattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     3121 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     3181 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagcttg catgcctgca
     3241 ggtcgactct agaggatccg atatcgaatt cctgcagccc gggggatcct acggtactta
     3301 ttgccaagaa agcacgagac gatatggcgt ccgtgcctcc aggtcgaatc ttcaaacgac
     3361 gagaggatat ggcctccgtc gctccagtat tccagggttc atcagatcct acggtactta
     3421 ttgccaagaa agcacgagac gatatggcgt ccgtgcctcc aggtcgaatc ttcaaacgac
     3481 gagaggatat ggcctccgtc gctccagtat tccagggttc atcagatcct acggtactta
     3541 ttgccaagaa agcacgagac gatatggcgt ccgtgcctcc aggtcgaatc ttcaaacgac
     3601 gagaggatat ggcctccgtc gctccagtat tccagggttc atcagatcct acggtactta
     3661 ttgccaagaa agcacgagac gatatggcgt ccgtgcctcc aggtcgaatc ttcaaacgac
     3721 gagaggatat ggcctccgtc gctccagtat tccagggttc atcagatcct acggtactta
     3781 ttgccaagaa agcacgagac gatatggcgt ccgtgcctcc aggtcgaatc ttcaaacgac
     3841 gagaggatat ggcctccgtc gctccagtat tccagggttc atcagatcct acggtactta
     3901 ttgccaagaa agcacgagac gatatggcgt ccgtgcctcc aggtcgaatc ttcaaacgac
     3961 gagaggatat ggcctccgtc gctccagtat tccagggttc atcagatcct acggtactta
     4021 ttgccaagaa agcacgagac gatatggcgt ccgtgcctcc aggtcgaatc ttcaaacgac
     4081 gagaggatat ggcctccgtc gctccagtat tccagggttc atcagatcct acggtactta
     4141 ttgccaagaa agcacgagac gatatggcgt ccgtgcctcc aggtcgaatc ttcaaacgac
     4201 gagaggatat ggcctccgtc gctccagtat tccagggttc atcagatcct acggtactta
     4261 ttgccaagaa agcacgagac gatatggcgt ccgtgcctcc aggtcgaatc ttcaaacgac
     4321 gagaggatat ggcctccgtc gctccagtat tccagggttc atcagatcct acggtactta
     4381 ttgccaagaa agcacgagac gatatggcgt ccgtgcctcc aggtcgaatc ttcaaacgac
     4441 gagaggatat ggcctccgtc gctccagtat tccagggttc atcagatcct acggtactta
     4501 ttgccaagaa agcacgagac gatatggcgt ccgtgcctcc aggtcgaatc ttcaaacgac
     4561 gagaggatat ggcctccgtc gctccagtat tccagggttc atcagatcct acggtactta
     4621 ttgccaagaa agcacgagac gatatggcgt ccgtgcctcc aggtcgaatc ttcaaacgac
     4681 gagaggatat ggcctccgtc gctccagtat tccagggttc atcagagtgc cagagtcctt
     4741 cgatagggac aagacaattg cactgatcat gaactcctct ggatctactg gtctgcctaa
     4801 aggtgtcgct ctgcctcata gaactgcctg cgtgagattc tcgcatgcca gagatcctat
     4861 ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg
     4921 ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta
     4981 tagatttgaa gaagagctgt ttctgaggag ccttcaggat tacaagattc aaagtgcgct
     5041 gctggtgcca accctattct ccttcttcgc caaaagcact ctgattgaca aatacgattt
     5101 atctaattta cacgaaattg cttctggtgg cgctcccctc tctaaggaag tcggggaagc
     5161 ggttgccaag aggttccatc tgccaggtat caggcaagga tatgggctca ctgagactac
     5221 atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt
     5281 tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg gcgttaatca
     5341 aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa acaatccgga
     5401 agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca tagcttactg
     5461 ggacgaagac gaacacttct tcatcgttga ccgcctgaag tctctgatta agtacaaagg
     5521 ctatcaggtg gctcccgctg aattggaatc catcttgctc caacacccca acatcttcga
     5581 cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt
     5641 tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt
     5701 aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct
     5761 taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg
     5821 aaagatcgcc gtgtatggtc cgtcctgtag aaaccccaac ccgtgaaatc aaaaaactcg
     5881 acggcctgtg ggcattcagt ctggatcgcg aaaactgtgg aattggtcag cgttggtggg
     5941 aaagcgcgtt acaagaaagc cgggcaattg ctgtgccagg cagttttaac gatcagttcg
     6001 ccgatgcaga tattcgtaat tatgcgggca acgtctggta tcagcgcgaa gtctttatac
     6061 cgaaaggttg ggcaggccag cgtatcgtgc tgcgtttcga tgcggtcact cattacggca
     6121 aagtgtgggt caataatcag gaagtgatgg agcatcaggg cggctatacg ccatttgaag
     6181 ccgatgtcac gccgtatgtt attgccggga aaagtctacg taagtttctg cttctacctt
     6241 tgatatatat ataataatta tcattaatta gtagtaatat aatatttcaa atattttttt
     6301 caaaataaaa gaatgtagta tatagcaatt gcttttctgt agtttataag tgtgtatatt
     6361 ttaatttata acttttctaa tatatgacca aaatttgttg atgtgcaggt atcaccgttt
     6421 gtgtgaacaa cgaactgaac tggcagacta tcccgccggg aatggtgatt accgacgaaa
     6481 acggcaagaa aaagcagtct tacttccatg atttctttaa ctatgccgga atccatcgca
     6541 gcgtaatgct ctacaccacg ccgaacacct gggtggacga tatcaccgtg gtgacgcatg
     6601 tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt ggtggccaat ggtgatgtca
     6661 gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc actagcggga
     6721 ctttgcaagt ggtgaatccg cacctctggc aaccgggtga aggttatctc tatgaactgt
     6781 gcgtcacagc caaaagccag acagagtgtg atatctaccc gcttcgcgtc ggcatccggt
     6841 cagtggcagt gaagggcgaa cagttcctga ttaaccacaa accgttctac tttactggct
     6901 ttggtcgtca tgaagatgcg gacttgcgtg gcaaaggatt cgataacgtg ctgatggtgc
     6961 acgaccacgc attaatggac tggattgggg ccaactccta ccgtacctcg cattaccctt
     7021 acgctgaaga gatgctcgac tgggcagatg aacatggcat cgtggtgatt gatgaaactg
     7081 ctgctgtcgg ctttaacctc tctttaggca ttggtttcga agcgggcaac aagccgaaag
     7141 aactgtacag cgaagaggca gtcaacgggg aaactcagca agcgcactta caggcgatta
     7201 aagagctgat agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt attgccaacg
     7261 aaccggatac ccgtccgcaa ggtgcacggg aatatttcgc gccactggcg gaagcaacgc
     7321 gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt aatgttctgc gacgctcaca
     7381 ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac ggatggtatg
     7441 tccaaagcgg cgatttggaa acggcagaga aggtactgga aaaagaactt ctggcctggc
     7501 aggagaaact gcatcagccg attatcatca ccgaatacgg cgtggatacg ttagccgggc
     7561 tgcactcaat gtacaccgac atgtggagtg aagagtatca gtgtgcatgg ctggatatgt
     7621 atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg
     7681 attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa caagaaaggg atcttcactc
     7741 gcgaccgcaa accgaagtcg gcggcttttc tgctgcaaaa acgctggact ggcatgaact
     7801 tcggtgaaaa accgcagcag ggaggcaaac aatgaatatg aagatgaaga tgaatatttg
     7861 gtgtgtcaaa taaaaagctt gtgtgcttaa gtttgtgttt ttttcttggc ttgttgtgtt
     7921 atgaatttgt ggctttttct aatattaaat gaatgtaaga tctcattata atgaataaac
     7981 aaatgtttct ataatccatt gtgaatgttt tgttggatct cttctgcagc atataactac
     8041 tgtatgtgct atggtatgga ctatggaata tgattaaaga taagatgggc tcatagagta
     8101 aaacgaggcg agggacctat aaacctcact tcatcatgct atttcatgat ctattttata
     8161 aaataaagat gtagaaaaaa gtaagcgtaa taaccgcaaa acaaatgatt taaaacatgg
     8221 cacataatga ggagattaag ttcggtttac gtttatttta gtactaattg taacgtgaga
     8281 ctacgtatcg ggaatcgcct accgggtacc gagctcgaat tcgtaatcat gtcatagctg
     8341 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8401 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8461 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     8521 gcggggagag gcggtttgcg tattggctag agcagcttgc caacatggtg gagcacgaca
     8581 ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga
     8641 cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc
     8701 acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata
     8761 aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     8821 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     8881 gatgtgaaca tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc
     8941 tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc
     9001 ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc
     9061 acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac
     9121 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
     9181 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
     9241 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
     9301 aggacacgct gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga
     9361 tctgtcgatc gaccatgggg attgaacaag atggattgca cgcaggttct ccggccgctt
     9421 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     9481 ccgtgtttcg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     9541 gtgccctgaa tgaactccag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     9601 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     9661 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     9721 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     9781 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     9841 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     9901 aggcgcgcat gcccgacggc gaggatctcg tcgtgacaca tggcgatgcc tgcttgccga
     9961 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
    10021 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
    10081 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
    10141 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg gatcgatcct
    10201 ctagctagag tcgatcgaca agctcgagtt tctccataat aatgtgtgag tagttcccag
    10261 ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt
    10321 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
    10381 aatccagtac taaaatccag atcccccgaa ttaattcggc gttaattcag tacattaaaa
    10441 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
    10501 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
    10561 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
    10621 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
    10681 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
    10741 tcaccgcggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa
    10801 ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga
    10861 gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg
    10921 aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac
    10981 cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa
    11041 aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg
    11101 gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg
    11161 cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt
    11221 tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag
    11281 tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac
    11341 agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa
    11401 aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg
    11461 gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt
    11521 gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag
    11581 tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat
    11641 gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat
    11701 attttactgg atgaattgtt ttagtaccta gaatgcatga ccaaaatccc ttaacgtgag
    11761 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    11821 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    11881 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    11941 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
    12001 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    12061 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    12121 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    12181 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    12241 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    12301 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    12361 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    12421 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    12481 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    12541 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
    12601 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
    12661 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
    12721 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
    12781 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
    12841 cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc
    12901 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
    12961 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
    13021 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
    13081 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
    13141 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
    13201 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
    13261 acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
    13321 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
    13381 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
    13441 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
    13501 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
    13561 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
    13621 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
    13681 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
    13741 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
    13801 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
    13861 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
    13921 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
    13981 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
    14041 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
    14101 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
    14161 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct
    14221 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
    14281 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
    14341 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
    14401 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag gcaacccagc
    14461 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
    14521 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
    14581 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
    14641 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
    14701 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
    14761 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
    14821 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
    14881 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
    14941 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
    15001 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
    15061 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
    15121 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
    15181 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
    15241 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
    15301 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
    15361 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
    15421 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
    15481 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
    15541 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
    15601 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac
    15661 gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt
    15721 aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt
    15781 tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg
    15841 cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc
    15901 gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc
    15961 agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg
    16021 cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg
    16081 aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg
    16141 gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
//
