LOCUS       sequence-319143-       17029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PP7-tagged reporter driven by the 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17029)
  AUTHORS   Alamos S, Reimer A, Niyogi KK, Garcia HG
  TITLE     Quantitative imaging of RNA polymerase II activity in plants reveals
            the single-cell basis of tissue-wide transcriptional dynamics.
  JOURNAL   Nat Plants. 2021 Aug;7(8):1037-1049. doi: 
            10.1038/s41477-021-00976-0. Epub 2021 Aug 9.
  PUBMED    34373604
REFERENCE   2  (bases 1 to 17029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-00976-0"; journalName: "Nat Plants"; date: 
            "2021-08"; volume: "7"; issue: "8"; pages: "1037-1049"
COMMENT     Sequence Label: AL13Rb-35S
FEATURES             Location/Qualifiers
     source          1..17029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    571..595
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     783..805
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     797..814
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     798..814
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1351..1696
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     intron          4654..4842
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     terminator      6271..6518
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             7870..8565
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     8271..8290
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(9170..9186)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9170..9186)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9183..9205)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9194..9210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9218..9224,9225..9242,9243..9248))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9263..9284
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9475..10151
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             10214..11011
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10271..10290)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10881..10900
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11068..11242
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11320..11344
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11769..12563
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12650..13238
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13139..13158
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13392..13409
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    13424..13564
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(13490..13509)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13650..13672
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      13908..14102
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14168..15241)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(15670..16299)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
       61 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
      121 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
      181 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
      241 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
      301 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
      361 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
      421 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
      481 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
      541 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
      601 tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      661 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      721 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      781 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtcga
      841 ctcttgcatg cctgcaggtc cccagattag ccttttcaat ttcagaaaga atgctaaccc
      901 acagatggtt agagaggctt acgcagcagg tctcatcaag acgatctacc cgagcaataa
      961 tctccaggaa atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac
     1021 taactgcatc aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt
     1081 atggacgatt caaggcttgc ttcacaaacc aaggcaagta atagagattg gagtctctaa
     1141 aaaggtagtt cccactgaat caaaggccat ggagtcaaag attcaaatag aggacctaac
     1201 agaactcgcc gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa
     1261 gaagaaaatc ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa
     1321 agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg
     1381 aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa
     1441 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc
     1501 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     1561 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
     1621 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     1681 tcatttggag agaacacggg ggactctaga ggatccggga tcctacggta cttattgcca
     1741 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     1801 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     1861 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     1921 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     1981 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     2041 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     2101 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     2161 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     2221 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     2281 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     2341 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     2401 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     2461 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     2521 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     2581 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     2641 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     2701 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     2761 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     2821 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     2881 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     2941 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     3001 atatggcctc cgtcgctcca gtattccagg gttcatcaga tcctacggta cttattgcca
     3061 agaaagcacg agacgatatg gcgtccgtgc ctccaggtcg aatcttcaaa cgacgagagg
     3121 atatggcctc cgtcgctcca gtattccagg gttcatcaga gtgccagagt ccttcgatag
     3181 ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt
     3241 cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg
     3301 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg
     3361 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt
     3421 tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt
     3481 gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa
     3541 tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc
     3601 caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc
     3661 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt
     3721 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg
     3781 cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac
     3841 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga
     3901 agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca
     3961 ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg
     4021 tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga
     4081 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac
     4141 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg
     4201 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat
     4261 cgccgtgtat ggtccgtcct gtagaaaccc caacccgtga aatcaaaaaa ctcgacggcc
     4321 tgtgggcatt cagtctggat cgcgaaaact gtggaattgg tcagcgttgg tgggaaagcg
     4381 cgttacaaga aagccgggca attgctgtgc caggcagttt taacgatcag ttcgccgatg
     4441 cagatattcg taattatgcg ggcaacgtct ggtatcagcg cgaagtcttt ataccgaaag
     4501 gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt cactcattac ggcaaagtgt
     4561 gggtcaataa tcaggaagtg atggagcatc agggcggcta tacgccattt gaagccgatg
     4621 tcacgccgta tgttattgcc gggaaaagtc tacgtaagtt tctgcttcta cctttgatat
     4681 atatataata attatcatta attagtagta atataatatt tcaaatattt ttttcaaaat
     4741 aaaagaatgt agtatatagc aattgctttt ctgtagttta taagtgtgta tattttaatt
     4801 tataactttt ctaatatatg accaaaattt gttgatgtgc aggtatcacc gtttgtgtga
     4861 acaacgaact gaactggcag actatcccgc cgggaatggt gattaccgac gaaaacggca
     4921 agaaaaagca gtcttacttc catgatttct ttaactatgc cggaatccat cgcagcgtaa
     4981 tgctctacac cacgccgaac acctgggtgg acgatatcac cgtggtgacg catgtcgcgc
     5041 aagactgtaa ccacgcgtct gttgactggc aggtggtggc caatggtgat gtcagcgttg
     5101 aactgcgtga tgcggatcaa caggtggttg caactggaca aggcactagc gggactttgc
     5161 aagtggtgaa tccgcacctc tggcaaccgg gtgaaggtta tctctatgaa ctgtgcgtca
     5221 cagccaaaag ccagacagag tgtgatatct acccgcttcg cgtcggcatc cggtcagtgg
     5281 cagtgaaggg cgaacagttc ctgattaacc acaaaccgtt ctactttact ggctttggtc
     5341 gtcatgaaga tgcggacttg cgtggcaaag gattcgataa cgtgctgatg gtgcacgacc
     5401 acgcattaat ggactggatt ggggccaact cctaccgtac ctcgcattac ccttacgctg
     5461 aagagatgct cgactgggca gatgaacatg gcatcgtggt gattgatgaa actgctgctg
     5521 tcggctttaa cctctcttta ggcattggtt tcgaagcggg caacaagccg aaagaactgt
     5581 acagcgaaga ggcagtcaac ggggaaactc agcaagcgca cttacaggcg attaaagagc
     5641 tgatagcgcg tgacaaaaac cacccaagcg tggtgatgtg gagtattgcc aacgaaccgg
     5701 atacccgtcc gcaaggtgca cgggaatatt tcgcgccact ggcggaagca acgcgtaaac
     5761 tcgacccgac gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct cacaccgata
     5821 ccatcagcga tctctttgat gtgctgtgcc tgaaccgtta ttacggatgg tatgtccaaa
     5881 gcggcgattt ggaaacggca gagaaggtac tggaaaaaga acttctggcc tggcaggaga
     5941 aactgcatca gccgattatc atcaccgaat acggcgtgga tacgttagcc gggctgcact
     6001 caatgtacac cgacatgtgg agtgaagagt atcagtgtgc atggctggat atgtatcacc
     6061 gcgtctttga tcgcgtcagc gccgtcgtcg gtgaacaggt atggaatttc gccgattttg
     6121 cgacctcgca aggcatattg cgcgttggcg gtaacaagaa agggatcttc actcgcgacc
     6181 gcaaaccgaa gtcggcggct tttctgctgc aaaaacgctg gactggcatg aacttcggtg
     6241 aaaaaccgca gcagggaggc aaacaatgaa tatgaagatg aagatgaata tttggtgtgt
     6301 caaataaaaa gcttgtgtgc ttaagtttgt gtttttttct tggcttgttg tgttatgaat
     6361 ttgtggcttt ttctaatatt aaatgaatgt aagatctcat tataatgaat aaacaaatgt
     6421 ttctataatc cattgtgaat gttttgttgg atctcttctg cagcatataa ctactgtatg
     6481 tgctatggta tggactatgg aatatgatta aagataagat gggctcatag agtaaaacga
     6541 ggcgagggac ctataaacct cacttcatca tgctatttca tgatctattt tataaaataa
     6601 agatgtagaa aaaagtaagc gtaataaccg caaaacaaat gatttaaaac atggcacata
     6661 atgaggagat taagttcggt ttacgtttat tttagtacta attgtaacgt gagactacgt
     6721 atcgggaatc gcctaccggg taccggctgg atcttatgac gaccgttaga aattgtggtt
     6781 gtcgacgagt cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca cgagtcgtgt
     6841 ttatcaactc aaagcacaaa tacttttcct caacctaaaa ataaggcaat tagccaaaaa
     6901 caactttgcg tgtaaacaac gctcaataca cgtgtcattt tattattagc tattgcttca
     6961 ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc ttctataaaa
     7021 caatacccaa agagctcttc ttcttcacaa ttcagatttc aatttctcaa aatcttaaaa
     7081 actttctctc aattctctct accgtgatca aggtaaattt ctgtgttcct tattctctca
     7141 aaatcttcga ttttgttttc gttcgatccc aatttcgtat atgttctttg gtttagattc
     7201 tgttaatctt agatcgaaga cgattttctg ggtttgatcg ttagatatca tcttaattct
     7261 cgattagggt ttcatagata tcatccgatt tgttcaaata atttgagttt tgtctaataa
     7321 ttactcttcg atttgtgatt tctatctaga tctggtgtta gtttctagtt tgtgcgatcg
     7381 aatttgtcga ttaatctgag tttttctgat taacagatgg cgaaggcaga taagaaacca
     7441 gcggagaaga aaccggcaga gaaaactccg gcagtcgaac cagcagcagc ggcagagaag
     7501 aaaccaaaag ccggaaagaa actccccaag gaaccagccg tcgccggaga caagaagaag
     7561 aagagatcaa agaagaacgt tgagacatac aagatctaca tcttcaaggt gttgaagcaa
     7621 gttcatccag acatcggaat ctcaagcaaa gccatgggaa tcatgaacag tttcatcaac
     7681 gatatctttg agaagcttgc tggtgagtct tcgaagcttg cgaggtacaa caagaagccg
     7741 acgattactt ccagggagat tcagactgcg gttagacttg tgttgcccgg tgagttggcg
     7801 aaacatgctg tttctgaagg gactaaggcg gttacgaagt ttacgagttc tgctggagct
     7861 ggagaattca tggtgagcaa gggcgaggca gtgatcaagg agttcatgcg gttcaaggtg
     7921 cacatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     7981 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     8041 tcctgggaca tcctgtcccc tcagttcatg tacggctcca gggccttcac caagcacccc
     8101 gccgacatcc ccgactacta taagcagtcc ttccccgagg gcttcaagtg ggagcgcgtg
     8161 atgaacttcg aggacggcgg cgccgtgacc gtgacccagg acacctccct ggaggacggc
     8221 accctgatct acaaggtgaa gctccgcggc accaacttcc ctcctgacgg ccccgtaatg
     8281 cagaagaaga caatgggctg ggaagcgtcc accgagcggt tgtaccccga ggacggcgtg
     8341 ctgaagggcg acattaagat ggccctgcgc ctgaaggacg gcggccgcta cctggcggac
     8401 ttcaagacca cctacaaggc caagaagccc gtgcagatgc ccggcgccta caacgtcgac
     8461 cgcaagttgg acatcacctc ccacaacgag gactacaccg tggtggaaca gtacgaacgc
     8521 tccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaggc tttttgtgat
     8581 ctgatgataa gtggttggtt cgtgtctcat gcacttggga ggtgatctat ttcacctggt
     8641 gtagtttgtg tttccgtcag ttggaaaaac ttatccctat cgatttcgtt ttcattttct
     8701 gcttttcttt tatgtacctt cgtttgggct tgtaacgggc ctttgtattt caactctcaa
     8761 taataatcca agtgcatgtt aaacaatttg tcatctgttt cggctttgat atactactgg
     8821 tgaagatggg ccgtactact gcatcacaac gaaaaataat aataagatga aaaacttgaa
     8881 gtggaaaaaa aaaaaaactt gaatgttcac tactactcat tgaccataat gtttaacata
     8941 catagctcaa tagtattttt gtgaatatgg caacacaaac agtccaaaac aattgtctct
     9001 tactatacca aaccaagggc gccgcttgtt tgccactctt tgtgtgcaat agtgtgatta
     9061 ccacatctcc acattcaata tattccctga attatctgac gattttgatg gctcactgtt
     9121 ttcccaagtc tggtacccca cctacatggc agctcgaatt cgtaatcatg tcatagctgt
     9181 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     9241 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     9301 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     9361 cggggagagg cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac
     9421 tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac
     9481 ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca
     9541 cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa
     9601 aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
     9661 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
     9721 atgtgaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
     9781 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
     9841 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
     9901 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca
     9961 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
    10021 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
    10081 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
    10141 ggacacgctg aaatcaccag tctctctcta caaatctatc tctctcgagc tttcgcagat
    10201 ctgtcgatcg accatgggga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
    10261 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
    10321 cgtgtttcgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
    10381 tgccctgaat gaactccagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
    10441 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
    10501 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
    10561 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
    10621 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
    10681 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
    10741 ggcgcgcatg cccgacggcg aggatctcgt cgtgacacat ggcgatgcct gcttgccgaa
    10801 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
    10861 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
    10921 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
    10981 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgg atcgatcctc
    11041 tagctagagt cgatcgacaa gctcgagttt ctccataata atgtgtgagt agttcccaga
    11101 taagggaatt agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
    11161 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
    11221 atccagtact aaaatccaga tcccccgaat taattcggcg ttaattcagt acattaaaaa
    11281 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
    11341 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
    11401 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
    11461 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
    11521 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
    11581 caccgcggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaacaac
    11641 tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag tgaattggag
    11701 ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga
    11761 aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc
    11821 gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa
    11881 atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg
    11941 aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc
    12001 actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt
    12061 gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt
    12121 gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca
    12181 gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa
    12241 actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg
    12301 aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg
    12361 tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt
    12421 ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg
    12481 tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata
    12541 ttttactgga tgaattgttt tagtacctag aatgcatgac caaaatccct taacgtgagt
    12601 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    12661 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    12721 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    12781 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    12841 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    12901 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    12961 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    13021 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    13081 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    13141 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    13201 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    13261 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    13321 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    13381 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
    13441 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
    13501 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
    13561 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
    13621 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
    13681 gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg
    13741 acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc
    13801 ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa
    13861 gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca
    13921 ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt
    13981 tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc
    14041 caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca
    14101 caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct
    14161 ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta
    14221 ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc
    14281 cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt
    14341 cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc
    14401 ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca
    14461 cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga
    14521 tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac
    14581 cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt
    14641 gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg
    14701 ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt
    14761 ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta
    14821 ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc
    14881 cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga
    14941 aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa
    15001 cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg
    15061 ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg
    15121 ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca
    15181 ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca
    15241 cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcagg caacccagcc
    15301 gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc
    15361 tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta
    15421 ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg
    15481 tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa
    15541 agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc
    15601 tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac
    15661 ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc
    15721 agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta
    15781 gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc
    15841 ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg
    15901 tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc
    15961 ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc
    16021 ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc
    16081 gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg
    16141 atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag
    16201 aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc
    16261 tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt
    16321 ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat
    16381 acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag
    16441 gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg
    16501 tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta
    16561 atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt
    16621 cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc
    16681 gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg
    16741 agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca
    16801 gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc
    16861 ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga
    16921 actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg
    16981 cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcg
//
