LOCUS       sequence-319147-       17819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PP7-tagged reporter driven by the Arabidopsis EF-Tu promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17819)
  AUTHORS   Alamos S, Reimer A, Niyogi KK, Garcia HG
  TITLE     Quantitative imaging of RNA polymerase II activity in plants reveals
            the single-cell basis of tissue-wide transcriptional dynamics.
  JOURNAL   Nat Plants. 2021 Aug;7(8):1037-1049. doi: 
            10.1038/s41477-021-00976-0. Epub 2021 Aug 9.
  PUBMED    34373604
REFERENCE   2  (bases 1 to 17819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-00976-0"; journalName: "Nat Plants"; date: 
            "2021-08"; volume: "7"; issue: "8"; pages: "1037-1049"
COMMENT     Sequence Label: AL12R-EF-Tu
FEATURES             Location/Qualifiers
     source          1..17819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    571..595
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(1190..1885)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(1465..1484)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     intron          7849..8037
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     terminator      9466..9713
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(9960..9976)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9960..9976)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9973..9995)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9984..10000
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10008..10014,10015..10032,10033..10038))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10053..10074
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10265..10941
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10878..10898
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11004..11801
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11061..11080)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11671..11690
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11858..12032
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12110..12134
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12559..13353
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13440..14028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13929..13948
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14182..14199
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    14214..14354
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(14280..14299)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14440..14462
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      14698..14892
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14958..16031)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(16460..17089)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
       61 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
      121 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
      181 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
      241 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
      301 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
      361 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
      421 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
      481 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
      541 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
      601 tgataccatg taggtggggt accagacttg ggaaaacagt gagccatcaa aatcgtcaga
      661 taattcaggg aatatattga atgtggagat gtggtaatca cactattgca cacaaagagt
      721 ggcaaacaag cggcgccctt ggtttggtat agtaagagac aattgttttg gactgtttgt
      781 gttgccatat tcacaaaaat actattgagc tatgtatgtt aaacattatg gtcaatgagt
      841 agtagtgaac attcaagttt tttttttttt ccacttcaag tttttcatct tattattatt
      901 tttcgttgtg atgcagtagt acggcccatc ttcaccagta gtatatcaaa gccgaaacag
      961 atgacaaatt gtttaacatg cacttggatt attattgaga gttgaaatac aaaggcccgt
     1021 tacaagccca aacgaaggta cataaaagaa aagcagaaaa tgaaaacgaa atcgataggg
     1081 ataagttttt ccaactgacg gaaacacaaa ctacaccagg tgaaatagat cacctcccaa
     1141 gtgcatgaga cacgaaccaa ccacttatca tcagatcaca aaaagcctac ttgtacagct
     1201 cgtccatgcc gccggtggag tggcggccct cggagcgttc gtactgttcc accacggtgt
     1261 agtcctcgtt gtgggaggtg atgtccaact tgcggtcgac gttgtaggcg ccgggcatct
     1321 gcacgggctt cttggccttg taggtggtct tgaagtccgc caggtagcgg ccgccgtcct
     1381 tcaggcgcag ggccatctta atgtcgccct tcagcacgcc gtcctcgggg tacaaccgct
     1441 cggtggacgc ttcccagccc attgtcttct tctgcattac ggggccgtca ggagggaagt
     1501 tggtgccgcg gagcttcacc ttgtagatca gggtgccgtc ctccagggag gtgtcctggg
     1561 tcacggtcac ggcgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
     1621 ggaaggactg cttatagtag tcggggatgt cggcggggtg cttggtgaag gccctggagc
     1681 cgtacatgaa ctgaggggac aggatgtccc aggagaaggg cagggggcca cccttggtca
     1741 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
     1801 cgaactcgtg gccgttcatg gagccctcca tgtgcacctt gaaccgcatg aactccttga
     1861 tcactgcctc gcccttgctc accatgaatt ctccagctcc agcagaactc gtaaacttcg
     1921 taaccgcctt agtcccttca gaaacagcat gtttcgccaa ctcaccgggc aacacaagtc
     1981 taaccgcagt ctgaatctcc ctggaagtaa tcgtcggctt cttgttgtac ctcgcaagct
     2041 tcgaagactc accagcaagc ttctcaaaga tatcgttgat gaaactgttc atgattccca
     2101 tggctttgct tgagattccg atgtctggat gaacttgctt caacaccttg aagatgtaga
     2161 tcttgtatgt ctcaacgttc ttctttgatc tcttcttctt cttgtctccg gcgacggctg
     2221 gttccttggg gagtttcttt ccggcttttg gtttcttctc tgccgctgct gctggttcga
     2281 ctgccggagt tttctctgcc ggtttcttct ccgctggttt cttatctgcc ttcgccatct
     2341 gttaatcaga aaaactcaga ttaatcgaca aattcgatcg cacaaactag aaactaacac
     2401 cagatctaga tagaaatcac aaatcgaaga gtaattattc gacaaaactc aaattatttg
     2461 aacaaatcgg atgatatcta tgaaacccta atcgagaatt aagatgatat ctaacgatca
     2521 aacccagaaa atcgtcttcg atctaagatt aacagaatct aaaccaaaga acatatacga
     2581 aattgggatc gaacgaaaac aaaatcgaag attttgagag aataaggaac acagaaattt
     2641 accttgatca cggtagagag aattgagaga aagtttttaa gattttgaga aattgaaatc
     2701 tgaattgtga agaagaagag ctctttgggt attgttttat agaagaagaa gaagaaaaga
     2761 cgaggacgac taggtcacga gaaagctaag gcggtgaagc aatagctaat aataaaatga
     2821 cacgtgtatt gagcgttgtt tacacgcaaa gttgtttttg gctaattgcc ttatttttag
     2881 gttgaggaaa agtatttgtg ctttgagttg ataaacacga ctcgtgtgtg ccggctgcaa
     2941 ccactttgac gccgtttatt actgactcgt cgacaaccac aatttctaac ggtcgtcata
     3001 agatccagcc ggtttctggt ttgcaacttt caggttccat ttgagtctat ggttcatcaa
     3061 gtgcgagaag agctaaaaac aatagcgaag ggtgattaca agccaccaag ttcggagaaa
     3121 agaaaacacg ggtctattgt tttcgctgcc atcaacttgc ctgctactca agttcacagt
     3181 cttcttgaaa aggtaaccaa ccaatttctt atactatcat ataaaaaaac aaaaggaata
     3241 ttgagacaag aactcttcaa ctgccgaaaa ctaaaggtta agtatgggct ttgttattaa
     3301 ttaatagatg ttattcttat cagttggctg cagcaaaccc aacaatgaga tcttttctag
     3361 agggaaagaa aaagagcata caggaaaaac ttgaacggtc tcacgtgacg ctcgcccaca
     3421 agagaagcca tggcgtagca actgtagcca gctatagtca gcacttgaac agagaggtac
     3481 ccgtagagct caccgagctc atctacaacg acaagatggc tgctctaaca gcccatgttg
     3541 gatctgtgga cggagagacc gtagtctcca agaacgaatg gccacatgtt acattgtgga
     3601 cagcggaagg cgttactgcg aaagaggcca acacgttacc tcagctttac ttagaaggaa
     3661 aggcgagccg cttggtgata gatcctccgg tgtcaatctc aggtcctctg gagtttttct
     3721 gaatacttga ttaaacatgg aagtttctct cttgagggag gttgctcgtg gaatgggaca
     3781 catatggttg ttataataaa ccatttccat tgtcatgaga ttttgaggtt aatatatact
     3841 ttacttgttc attattttat ttggtgtttg aataaatgat ataaatggct cttgataatc
     3901 tgcattcatt gagatatcaa atatttactc tagagaagag tgtcatatag attgatggtc
     3961 cacaatcaat gaaatttttg ggagacgaac atgtataacc atttgcttga ataaccttaa
     4021 ttaaaaggtg tgattaaatg atgtttgtaa catgtagtac taaacattca taaaacacaa
     4081 ccaacccaag aggtattgag tattcacggc taaacagggg cataatggta atttaaagaa
     4141 tgatattatt ttatgttaaa ccctaacatt ggtttcggat tcaacgctat aaataaaacc
     4201 actctcgttg ctgattccat ttatcgttct tattgaccct agccgctaca cacttttctg
     4261 cgatatctct gaggtaagcg ttaacgtacc cttagatcgt tctttttctt tttcgtctgc
     4321 tgatcgttgc tcatattatt tcgatgattg ttggattcga tgctctttgt tgattgatcg
     4381 ttctgaaaat tctgatctgt tgtttagatt ttatcgattg ttaatatcaa cgtttcactg
     4441 cttctaaacg ataatttatt catgaaacta ttttcccatt ctgatcgatc ttgttttgag
     4501 attttaattt gttcgattga ttgttggttg gtggatctat atacgagtga acttgttgat
     4561 ttgcgtattt aagatgtatg tcgatttgaa ttgtgattgg gtaattctgg agtagcataa
     4621 caaatccagt gttccctttt tctaagggta attctcggat tgtttgcttt atatctcttg
     4681 aaattgccga tttgattgaa tttagctcgc ttagctcaga tgatagagca ccacaatttt
     4741 tgtggtagaa atcggtttga ctccgatagc ggctttttac tatgattgtt ttgtgttaaa
     4801 gatgattttc ataatggtta tatatgtcta ctgtttttat tgattcaata tttgattgtt
     4861 cttttttttg cagatttgtt gacagtctct aaccatgggt aaagagaagt agggatccta
     4921 cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca ggtcgaatct
     4981 tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca tcagatccta
     5041 cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca ggtcgaatct
     5101 tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca tcagatccta
     5161 cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca ggtcgaatct
     5221 tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca tcagatccta
     5281 cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca ggtcgaatct
     5341 tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca tcagatccta
     5401 cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca ggtcgaatct
     5461 tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca tcagatccta
     5521 cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca ggtcgaatct
     5581 tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca tcagatccta
     5641 cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca ggtcgaatct
     5701 tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca tcagatccta
     5761 cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca ggtcgaatct
     5821 tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca tcagatccta
     5881 cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca ggtcgaatct
     5941 tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca tcagatccta
     6001 cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca ggtcgaatct
     6061 tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca tcagatccta
     6121 cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca ggtcgaatct
     6181 tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca tcagatccta
     6241 cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca ggtcgaatct
     6301 tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca tcagagtgcc
     6361 agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg
     6421 tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag
     6481 agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt
     6541 ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt
     6601 cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca
     6661 aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa
     6721 atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt
     6781 cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac
     6841 tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg
     6901 taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg
     6961 cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa
     7021 caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat
     7081 agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa
     7141 gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa
     7201 catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc
     7261 cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc
     7321 cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc
     7381 gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa
     7441 gaagggcgga aagatcgccg tgtatggtcc gtcctgtaga aaccccaacc cgtgaaatca
     7501 aaaaactcga cggcctgtgg gcattcagtc tggatcgcga aaactgtgga attggtcagc
     7561 gttggtggga aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc agttttaacg
     7621 atcagttcgc cgatgcagat attcgtaatt atgcgggcaa cgtctggtat cagcgcgaag
     7681 tctttatacc gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat gcggtcactc
     7741 attacggcaa agtgtgggtc aataatcagg aagtgatgga gcatcagggc ggctatacgc
     7801 catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa aagtctacgt aagtttctgc
     7861 ttctaccttt gatatatata taataattat cattaattag tagtaatata atatttcaaa
     7921 tatttttttc aaaataaaag aatgtagtat atagcaattg cttttctgta gtttataagt
     7981 gtgtatattt taatttataa cttttctaat atatgaccaa aatttgttga tgtgcaggta
     8041 tcaccgtttg tgtgaacaac gaactgaact ggcagactat cccgccggga atggtgatta
     8101 ccgacgaaaa cggcaagaaa aagcagtctt acttccatga tttctttaac tatgccggaa
     8161 tccatcgcag cgtaatgctc tacaccacgc cgaacacctg ggtggacgat atcaccgtgg
     8221 tgacgcatgt cgcgcaagac tgtaaccacg cgtctgttga ctggcaggtg gtggccaatg
     8281 gtgatgtcag cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact ggacaaggca
     8341 ctagcgggac tttgcaagtg gtgaatccgc acctctggca accgggtgaa ggttatctct
     8401 atgaactgtg cgtcacagcc aaaagccaga cagagtgtga tatctacccg cttcgcgtcg
     8461 gcatccggtc agtggcagtg aagggcgaac agttcctgat taaccacaaa ccgttctact
     8521 ttactggctt tggtcgtcat gaagatgcgg acttgcgtgg caaaggattc gataacgtgc
     8581 tgatggtgca cgaccacgca ttaatggact ggattggggc caactcctac cgtacctcgc
     8641 attaccctta cgctgaagag atgctcgact gggcagatga acatggcatc gtggtgattg
     8701 atgaaactgc tgctgtcggc tttaacctct ctttaggcat tggtttcgaa gcgggcaaca
     8761 agccgaaaga actgtacagc gaagaggcag tcaacgggga aactcagcaa gcgcacttac
     8821 aggcgattaa agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg atgtggagta
     8881 ttgccaacga accggatacc cgtccgcaag gtgcacggga atatttcgcg ccactggcgg
     8941 aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta atgttctgcg
     9001 acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac cgttattacg
     9061 gatggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa aaagaacttc
     9121 tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc gtggatacgt
     9181 tagccgggct gcactcaatg tacaccgaca tgtggagtga agagtatcag tgtgcatggc
     9241 tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa caggtatgga
     9301 atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac aagaaaggga
     9361 tcttcactcg cgaccgcaaa ccgaagtcgg cggcttttct gctgcaaaaa cgctggactg
     9421 gcatgaactt cggtgaaaaa ccgcagcagg gaggcaaaca atgaatatga agatgaagat
     9481 gaatatttgg tgtgtcaaat aaaaagcttg tgtgcttaag tttgtgtttt tttcttggct
     9541 tgttgtgtta tgaatttgtg gctttttcta atattaaatg aatgtaagat ctcattataa
     9601 tgaataaaca aatgtttcta taatccattg tgaatgtttt gttggatctc ttctgcagca
     9661 tataactact gtatgtgcta tggtatggac tatggaatat gattaaagat aagatgggct
     9721 catagagtaa aacgaggcga gggacctata aacctcactt catcatgcta tttcatgatc
     9781 tattttataa aataaagatg tagaaaaaag taagcgtaat aaccgcaaaa caaatgattt
     9841 aaaacatggc acataatgag gagattaagt tcggtttacg tttattttag tactaattgt
     9901 aacgtgagac tacgtatcgg gaatcgccta ccgggtaccg agctcgaatt cgtaatcatg
     9961 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
    10021 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
    10081 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
    10141 ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc aacatggtgg
    10201 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg
    10261 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag
    10321 ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc
    10381 attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg
    10441 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
    10501 aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa
    10561 gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga
    10621 aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag
    10681 gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc
    10741 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
    10801 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
    10861 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
    10921 catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc tctctcgagc
    10981 tttcgcagat ctgtcgatcg accatgggga ttgaacaaga tggattgcac gcaggttctc
    11041 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
    11101 ctgatgccgc cgtgtttcgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
    11161 acctgtccgg tgccctgaat gaactccagg acgaggcagc gcggctatcg tggctggcca
    11221 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
    11281 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
    11341 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
    11401 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
    11461 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
    11521 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacacat ggcgatgcct
    11581 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
    11641 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
    11701 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
    11761 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgg
    11821 atcgatcctc tagctagagt cgatcgacaa gctcgagttt ctccataata atgtgtgagt
    11881 agttcccaga taagggaatt agggttccta tagggtttcg ctcatgtgtt gagcatataa
    11941 gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc
    12001 taaaaccaaa atccagtact aaaatccaga tcccccgaat taattcggcg ttaattcagt
    12061 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac
    12121 aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac
    12181 cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc
    12241 ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt
    12301 tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc
    12361 tgcctgtgat caccgcggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt
    12421 tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag
    12481 tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa
    12541 agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc
    12601 gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg
    12661 gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc
    12721 tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca
    12781 aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat
    12841 ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg
    12901 tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat
    12961 agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg
    13021 gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt
    13081 ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc
    13141 aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg
    13201 gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa
    13261 gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata
    13321 aaatattata ttttactgga tgaattgttt tagtacctag aatgcatgac caaaatccct
    13381 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    13441 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    13501 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    13561 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
    13621 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    13681 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    13741 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    13801 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    13861 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    13921 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    13981 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    14041 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    14101 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    14161 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
    14221 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
    14281 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
    14341 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
    14401 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
    14461 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg
    14521 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc
    14581 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc
    14641 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat
    14701 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa
    14761 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc
    14821 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac
    14881 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct
    14941 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag
    15001 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg
    15061 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg
    15121 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg
    15181 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga
    15241 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc
    15301 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc
    15361 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc
    15421 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt
    15481 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg
    15541 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc
    15601 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc
    15661 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc
    15721 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag
    15781 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc
    15841 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac
    15901 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc
    15961 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg
    16021 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcagg
    16081 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg
    16141 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc
    16201 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc
    16261 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc
    16321 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca
    16381 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt
    16441 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga
    16501 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag
    16561 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg
    16621 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa
    16681 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt
    16741 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg
    16801 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt
    16861 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg
    16921 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat
    16981 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg
    17041 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt
    17101 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt
    17161 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct
    17221 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac
    17281 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat
    17341 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc
    17401 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg
    17461 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg
    17521 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc
    17581 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca
    17641 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg
    17701 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg
    17761 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcg
//
