LOCUS       sequence-319149-       11372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ubiquitous expression of PCP-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11372)
  AUTHORS   Alamos S, Reimer A, Niyogi KK, Garcia HG
  TITLE     Quantitative imaging of RNA polymerase II activity in plants reveals
            the single-cell basis of tissue-wide transcriptional dynamics.
  JOURNAL   Nat Plants. 2021 Aug;7(8):1037-1049. doi: 
            10.1038/s41477-021-00976-0. Epub 2021 Aug 9.
  PUBMED    34373604
REFERENCE   2  (bases 1 to 11372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-00976-0"; journalName: "Nat Plants"; date: 
            "2021-08"; volume: "7"; issue: "8"; pages: "1037-1049"
COMMENT     Sequence Label: UPmCh
FEATURES             Location/Qualifiers
     source          1..11372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    384..408
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     596..618
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     610..627
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     611..627
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1745..2449
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     CDS             2456..2473
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3110..3126)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3110..3126)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3123..3145)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3134..3150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3158..3164,3165..3182,3183..3188))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3203..3224
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3415..4091
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     4028..4048
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             4158..5183
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5224..5398
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5476..5500
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5925..6719
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6806..7394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7295..7314
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7548..7565
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7580..7720
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7646..7665)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7806..7828
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8064..8258
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8324..9397)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9826..10455)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc
       61 ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa
      121 cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac
      181 ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta
      241 tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat
      301 gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc
      361 taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt
      421 ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca
      481 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
      541 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
      601 tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt cgactctaga
      661 ggatccagga tgatttctgg tttaaacaag cttcggctgg atcttatgac gaccgttaga
      721 aattgtggtt gtcgacgagt cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca
      781 cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct caacctaaaa ataaggcaat
      841 tagccaaaaa caactttgcg tgtaaacaac gctcaataca cgtgtcattt tattattagc
      901 tattgcttca ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc
      961 ttctataaaa caatacccaa agagctcttc ttcttcacaa ttcagatttc aatttctcaa
     1021 aatcttaaaa actttctctc aattctctct accgtgatca aggtaaattt ctgtgttcct
     1081 tattctctca aaatcttcga ttttgttttc gttcgatccc aatttcgtat atgttctttg
     1141 gtttagattc tgttaatctt agatcgaaga cgattttctg ggtttgatcg ttagatatca
     1201 tcttaattct cgattagggt ttcatagata tcatccgatt tgttcaaata atttgagttt
     1261 tgtcgaataa ttactcttcg atttgtgatt tctatctaga tctggtgtta gtttctagtt
     1321 tgtgcgatcg aatttgtcga ttaatctgag tttttctgat taacagatgc tagcctccaa
     1381 aaccatcgtt ctttcggtcg gcgaggctac tcgcactctg actgagatcc agtccaccgc
     1441 agaccgtcag atcttcgaag agaaggtcgg gcctctggtg ggtcggctgc gcctcacggc
     1501 ttcgctccgt caaaacggag ccaagaccgc gtatcgcgtc aacctaaaac tggatcaggc
     1561 ggacgtcgtt gattccggac ttccgaaagt gcgctacact caggtatggt cgcacgacgt
     1621 gacaatcgtt gcgaatagca ccgaggcctc gcgcaaatcg ttgtacgatt tgaccaagtc
     1681 cctcgtcgcg acctcgcagg tcgaagatct tgtcgtcaac cttgtgccgc tgggccgtgg
     1741 atccgtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     1801 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     1861 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     1921 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     1981 ccccgccgac atccccgact acctcaagct gtccttcccc gagggcttca agtgggagcg
     2041 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     2101 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     2161 catgcagaag aagaccatgg gctgggaggc ctcctccgag cgcatgtacc ccgaggacgg
     2221 cgccctgaag ggcgagatca agcagcgcct gaagctgaag gacggcggcc actacgacgc
     2281 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     2341 caacatcaag ctcgacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     2401 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg gcagccacca
     2461 ccaccaccac cactaggctt tttgtgatct gatgataagt ggttggttcg tgtctcatgc
     2521 acttgggagg tgatctattt cacctggtgt agtttgtgtt tccgtcagtt ggaaaaactt
     2581 atccctatcg atttcgtttt cattttctgc ttttctttta tgtaccttcg tttgggcttg
     2641 taacgggcct ttgtatttca actctcaata ataatccaag tgcatgttaa acaatttgtc
     2701 atctgtttcg gctttgatat actactggtg aagatgggcc gtactactgc atcacaacga
     2761 aaaataataa taagatgaaa aacttgaagt ggaaaaaaaa aaaaacttga atgttcacta
     2821 ctactcattg accataatgt ttaacataca tagctcaata gtatttttgt gaatatggca
     2881 acacaaacag tccaaaacaa ttgtctctta ctataccaaa ccaagggcgc cgcttgtttg
     2941 ccactctttg tgtgcaatag tgtgattacc acatctccac attcaatata ttccctgaat
     3001 tatctgacga ttttgatggc tcactgtttt cccaagtctg gtaccccacc tacatgggga
     3061 tcctaactcg agctgcaggc ttcggtaccg agctcgaatt cgtaatcatg tcatagctgt
     3121 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     3181 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     3241 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     3301 cggggagagg cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac
     3361 tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac
     3421 ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca
     3481 cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa
     3541 aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
     3601 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
     3661 atgtgaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
     3721 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
     3781 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
     3841 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca
     3901 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
     3961 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
     4021 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
     4081 ggacacgctg aaatcaccag tctctctcta caaatctatc tctctcgagc tttcgcagat
     4141 ccggggggca atgagatatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     4201 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     4261 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     4321 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     4381 cggaagtgct tgacattggg gagtttagcg agagcctgac ctattgcatc tcccgccgtt
     4441 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctacaaccgg
     4501 tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     4561 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     4621 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     4681 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     4741 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     4801 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     4861 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     4921 agcttgcagg atcgccacga ctccgggcgt atatgctccg cattggtctt gaccaactct
     4981 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     5041 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     5101 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     5161 ctcgtccgag ggcaaagaaa tagagtagat gccgaccggg atctgtcgat cgacaagctc
     5221 gagtttctcc ataataatgt gtgagtagtt cccagataag ggaattaggg ttcctatagg
     5281 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
     5341 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatccc
     5401 ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg
     5461 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
     5521 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
     5581 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
     5641 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
     5701 tgattgtaac gatgacagag cgttgctgcc tgtgatcacc gcggtttcaa aatcggctcc
     5761 gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat
     5821 ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct
     5881 tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa
     5941 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
     6001 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
     6061 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
     6121 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
     6181 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
     6241 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
     6301 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
     6361 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta
     6421 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
     6481 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
     6541 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
     6601 ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
     6661 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt
     6721 acctagaatg catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6781 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6841 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6901 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     6961 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7021 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7081 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7141 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7201 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7261 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7321 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7381 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7441 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7501 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7561 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     7621 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     7681 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     7741 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     7801 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     7861 gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg
     7921 gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac
     7981 gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt
     8041 cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa
     8101 gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca
     8161 tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa
     8221 tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt
     8281 tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg
     8341 cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct
     8401 cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac
     8461 agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc
     8521 tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg
     8581 gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga
     8641 tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc
     8701 tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc
     8761 cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg
     8821 tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt
     8881 ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt
     8941 tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg
     9001 ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca
     9061 cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc
     9121 gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc
     9181 ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc
     9241 ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt
     9301 gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc
     9361 cgatttgtac cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt
     9421 tccagtgcca ttgcagggcc ggcaggcaac ccagccgctt acgcctggcc aaccgcccgt
     9481 tcctccacac atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc
     9541 gcctccttta gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta
     9601 gctgcgcgat gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca
     9661 tcttcagctt ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg
     9721 tgtctgccag gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac
     9781 ttagcgtgtt tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga
     9841 tttaatttca gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc
     9901 aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga
     9961 ctcaagaatg ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt
    10021 gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat
    10081 gcgctgctta accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg
    10141 caccggaatc agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg
    10201 gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat
    10261 cgtcgggcgg tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc
    10321 gcgggcactg ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag
    10381 ggcgcgggct agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac
    10441 agcgataacc ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac
    10501 gcagcgaccg catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg
    10561 cgctcggttt cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa
    10621 ccggccagga tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac
    10681 ccggccgcga tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg
    10741 tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt
    10801 cggcctcggt caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca
    10861 cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag
    10921 ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg
    10981 ccggggtgag ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc
    11041 cgctccgggt gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg
    11101 tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca
    11161 ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg
    11221 ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc
    11281 tggccagctc cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc
    11341 agccggccgc gtgcagttcg gcccgttggt tg
//
