LOCUS       sequence-319160-       18097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal mCherry fusion to Arabidopsis HSP70 with intronic PP7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18097)
  AUTHORS   Alamos S, Reimer A, Niyogi KK, Garcia HG
  TITLE     Quantitative imaging of RNA polymerase II activity in plants reveals
            the single-cell basis of tissue-wide transcriptional dynamics.
  JOURNAL   Nat Plants. 2021 Aug;7(8):1037-1049. doi: 
            10.1038/s41477-021-00976-0. Epub 2021 Aug 9.
  PUBMED    34373604
REFERENCE   2  (bases 1 to 18097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-00976-0"; journalName: "Nat Plants"; date: 
            "2021-08"; volume: "7"; issue: "8"; pages: "1037-1049"
COMMENT     Sequence Label: HSP70-pp7i-mCh-UPG
FEATURES             Location/Qualifiers
     source          1..18097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    571..595
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     783..805
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     797..814
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     798..814
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6366..7070
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     CDS             7077..7094
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      7099..7347
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             8664..9374
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(8691..8719)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     9314..9334
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(10022..10038)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10022..10038)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10035..10057)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10046..10062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10070..10076,10077..10094,10095..10100))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10115..10136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10327..11003
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10940..10960
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11070..12095
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    12136..12310
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12388..12412
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12837..13631
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13718..14306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14207..14226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14460..14477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    14492..14632
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(14558..14577)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14718..14740
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      14976..15170
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(15236..16309)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(16738..17367)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
       61 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
      121 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
      181 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
      241 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
      301 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
      361 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
      421 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
      481 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
      541 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
      601 tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      661 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      721 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      781 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtcga
      841 ctctctgttc tgcaaatcgc ttctggtccc tggagtctgg agatctcatc ctttcccacc
      901 gattccgtcc cgataaaacg gtaaatctcg ctcaatttta aaactatgta tatatatgca
      961 tttcttcttg agtgatcttc tgattcgacg acaagtgtgt ttgatttatg atctggttag
     1021 tcttagactc ttagctgttc ttctcgcgat agagattgtc tctgtggcgg ccatctgtgt
     1081 gtgatggtcc tttgaatccg tctctgtcga cgagaactgc caaattctct tgtcggattg
     1141 tgtcacgttt taggttttta cataaattct caacttgttc tccaatgtga tcccatagcg
     1201 gagtggatat cgcgttagac tccgaatcta aaggtcgtgg gttcgattcc cactgggatc
     1261 atcccatttt tttggtcttt ttttttgttg gttattaatt attactatca atccgttctt
     1321 ttagcagtga tcaagcgaat ttaggtctgg ctctgcctct ggctctagtt gagtttaggg
     1381 agctgctcct tgttgtttgc cattatggtt tctctacttt ggagtcaact ttgcaatgtt
     1441 acccttgtag tctcttctgt ggactttgta tttggtgaaa ttgaaaagtt tagtagaaat
     1501 ctggctgcgc caaagatgat agagactcta atgggatcct tattcgaaca ttttgctgaa
     1561 ctgattagat acaataaggc aacactgatt ccattactgt tagtctgtta ctacaaaaag
     1621 aattgttgct caagtattgt ttgtgttggt tggtgcagat gatcgatgat caggacttgg
     1681 ggtttattgc caactttctt ggcatcttca tcttcgcatt ggtaattgct tatcactacg
     1741 taactgctga tcccaaatac gaagccactt gagtgatgat attttagaat gatgtaaggc
     1801 tttttagttt atactagtat tatctgtgtt tcaaactgag aagagataat aacagtcttt
     1861 gttgagatga taatgttttc aagatgttcc taatccattt cacatcttct caattttata
     1921 tgcatgtgca tatatatgtt ccctccaatt atgttgttcg aatgtttgat gaaactttga
     1981 atttttttct ttaagcaaaa aaaaatctca aacaccaaag cgaggagtca ttctagttca
     2041 gttttgagtc attctagtta tttttacaaa gttttgagtc aaattgggta aattttttgg
     2101 ttattttggt cataaaaata actagattat ctcttatatc ttatgagtta atttggtaaa
     2161 taaaccattt atttgggtca aactattttt ttccccatat atatatccaa tcaataataa
     2221 attcataata tatttcatta acgcgattga aatactagta attaattgag gactaaagaa
     2281 aaagtaattt cctttttatc tttaaaatgt gcaaaaaaaa caaaaatgtt aattgggtga
     2341 tgaaataact tgttttcaaa acgggagtta ctatttgaca atttaaaaaa gacccatctc
     2401 gaaggagcta gaagcgataa caaaataaaa aggaaacaat agtaattaga tggcgcaaaa
     2461 ataagatcca acggctgaga tctttactcg tgaacgttct cgaaagctct ttgccgaccc
     2521 actcttcatt catatataaa caaacacctc tctgccttct cttcctcaca caatcataaa
     2581 cacaacaaca ctcacaaatt ctcttaaagc tcacagacga attctttcta tttttaatct
     2641 ttccggcgaa caattctgat ctctaataat ggcgggtaaa ggtgaaggtc cagctatcgg
     2701 tatcgatctc ggtacaacct actcttgcgt cggtgtttgg caacatgacc gcgtcgaaat
     2761 catcgccaac gatcaaggca accgcaccac tccttcctac gttgctttca ctgacagcga
     2821 gcgtctcatc ggggatgctg ccaagaatca agtcgccatg aaccctacca acaccgtctt
     2881 cggtaaacat ctcacttcct tctcatctta cttactatat ctgctttgct tcctgcatgt
     2941 gattaagttt gtgtaactca ttgctcgaac ctacaaaccg gctgaggtta tatggaaccc
     3001 agccccacac ttcacaatca aaggggtaca atacacaagt atagtgtcta cacggagacc
     3061 tatactccag acacatttac acagaaatcg atacaaacat aattaatgga tatggccata
     3121 attatcctac gaaatttatc tccaaaaaac tacaaacagg aagacgtata cacggatact
     3181 cttccgtctg caatatactt tcaaacgacg cccacacctt gtgcagtata cagggatacg
     3241 cacacccata tcggcatttc gtcgcattcg aattggacat aattatcgta tatggacgaa
     3301 attagaccga ttcagagtcg aaatagaact cgactttcgg aagcgcttga tacagcaagg
     3361 cttcgcccat tccagaaacc aaccatagtc tccccttgta ttcgagtaaa tatggttacc
     3421 gaatagccca gtacccaaca ctagaaaatt ttaactcccg gagcagacga tatggcgtcg
     3481 ctccggaccc aaagaataca tccccttcaa aatcaccttc gacgagagga tatggcctcc
     3541 gtcgatgcga tgttcctgtg gatagagctt caaactgtac gcacgagacg atatggcgtc
     3601 cgtgcgcctg tcaccaacaa agacaaaaac tactcttctc ggctgaggtt atatggaacc
     3661 cagccgaaca ctgctgcata caattgtgcc tatttcttgt atagtgtcta cacggagacc
     3721 tatacatcgc ctagccttaa ttcaaaacac ggacaagcct aattaatgga tatggccata
     3781 attatcgttc catggccaaa tacgattact tagttatcag aagacgtata cacggatact
     3841 cttccgcacc ttcatctgtt cccactcagc acattcatct gtgcagtata cagggatacg
     3901 cacagtccac aagtctcaaa ccacagaaac tagagtttct aattatcgta tatggacgaa
     3961 attacagtcg aaagtcacac ctctttccca caaaactgcg aagcgcttga tacagcaagg
     4021 cttcgccatt cgaactaagg caaatcaaaa acaattagta ttcgagtaaa tatggttacc
     4081 gaataccacg cattcagtga ctactatcaa ataccaaacc cagcagagca tatgggctcg
     4141 ctgggtacac acggaaaaat tcatctggta tacaaacatc gacgagagga tatggcctcc
     4201 gtcgaactat tacgagttgg tttctgaaaa catccgagcg cacgagacga tatggcgtcc
     4261 gtgcctattc ttaaaaatct tacaaaaata cttacaattc gagcagacga tatggcgtcg
     4321 ctcgcattga taacttataa acttacaaat gacttaaatg gctgaggtta tatggaaccc
     4381 agccgtacat caaacattta tcttctaatt gcgcgctata gttatcagtt ctttatgtct
     4441 gtacgtacta gcgatcggtc catagtgatt ttggtttatg aatcggttat tcaattttgg
     4501 ttatatcgaa acagagccgg tttagtgtgt caagcagata gaaatctgtt agagtttatt
     4561 agtgttctct atgttgttgt taattaggag gaagtaagta ttaaacttgt tgttgtgttt
     4621 tttttgcaga tgctaagcgt ctaatcggaa gaagatacag tgatccctct gttcaagcgg
     4681 ataagagtca ctggcctttt aaggttgttt ccggtccagg tgagaagcct atgattgtgg
     4741 ttaaccacaa gggagaggag aaacagttct ctgctgagga aatctcgtcg atggttctta
     4801 ttaagatgcg ggagattgca gaagctttcc ttggttctcc tgttaagaac gctgtcgtta
     4861 cagttcctgc ttatttcaac gactctcagc gtcaagcgac taaggacgct ggagttatct
     4921 ctggtctcaa cgtgatgcgt atcatcaatg agccaactgc tgctgctatt gcttacggtc
     4981 ttgacaagaa ggcgtcgagt gttggcgaga agaatgtttt gatctttgat ttgggaggtg
     5041 gtacttttga tgtgtctttg cttacgattg aggaaggtat ctttgaagtc aaggcaactg
     5101 ctggtgacac gcatcttggt ggtgaggact tcgacaacag gatggttaat cattttgttc
     5161 aggagtttaa gaggaagaac aagaaggata ttactgggaa cccgagagct ttgaggaggc
     5221 ttaggacagc ttgtgagcgg gcgaagagaa ctctttcttc gactgctcag acgactatag
     5281 agattgactc tctttttgag ggtattgatt tctacactac catcactcgt gctaggttcg
     5341 aggagctcaa catggatttg tttaggaagt gtatggagcc agtggagaag tgtttgaggg
     5401 atgctaagat ggacaagagc agtgttcatg atgttgttct tgttggtggc tctacaagga
     5461 ttcccaaagt gcagcagctt ttgcaagact tcttcaatgg gaaagagctc tgtaaaagca
     5521 ttaacccgga cgaggctgtt gcttacggag cagctgtgca agctgcaatc ttgagcggtg
     5581 aagggaatga gaaggtccag gacttgttac ttcttgatgt cactcctctg tccttgggtt
     5641 tggaaactgc cggtggtgtt atgactgttt tgattccgag gaacaccaca attccgacca
     5701 agaaagagca gatattctct acctattcag acaaccagcc cggtgtactg atccaggtct
     5761 acgaaggaga gagggcacga acaaaggaca acaacctttt gggaaagttc gagctcagtg
     5821 gtataccacc tgctccacga ggtgtaccgc agattactgt ctgtttcgac atcgacgcca
     5881 atggtatcct gaatgtgtcg gctgaggaca agacgactgg tcagaagaac aagatcacaa
     5941 tcacaaacga caagggaagg ttatcaaagg aagagatcga gaagatggta caagaggcag
     6001 agaagtacaa ggctgaggat gaagaacaca agaagaaggt ggatgcaaag aacgctctcg
     6061 agaactatgc atacaacatg aggaacacga tcaaggacga gaagatcgca tctaagcttg
     6121 acgcagctga caagaagaag attgaggatg caatcgacca agctattgaa tggttagatg
     6181 ggaatcaact ggctgaggca gatgagttcg aggataagat gaaggagctc gagtctcttt
     6241 gcaaccctat tattgcaaga atgtaccaag gagctgggcc tgatatgggt ggtgcaggag
     6301 gaatggatga cgacacacct gctggtggta gcggcggtgc tggcccaaag attgaagaag
     6361 ttgatgtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     6421 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     6481 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     6541 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     6601 accccgccga catccccgac tacctcaagc tgtccttccc cgagggcttc aagtgggagc
     6661 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     6721 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     6781 tcatgcagaa gaagaccatg ggctgggagg cctcctccga gcgcatgtac cccgaggacg
     6841 gcgccctgaa gggcgagatc aagcagcgcc tgaagctgaa ggacggcggc cactacgacg
     6901 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     6961 tcaacatcaa gctcgacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     7021 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag ggcagccacc
     7081 accaccacca ccactgaata tgaagatgaa gatgaaatat ttggtgtgtc aaataaaaag
     7141 cttgtgtgct taagtttgtg tttttttctt ggcttgttgt gttatgaatt tgtggctttt
     7201 tctaatatta aatgaatgta agatctcatt ataatgaata aacaaatgtt tctataatcc
     7261 attgtgaatg ttttgttgga tctcttctgc agcatataac tactgtatgt gctatggtat
     7321 ggactatgga atatgattaa agataagatg ggctcataga gtaaaacgag gcgagggacc
     7381 tataaacctc ccttcatcat gctatttcat gatctatttt ataaaataaa gatgtagaaa
     7441 aaagtaagcg taataaccgc aaaacaaatg atttaaaaca tggcacataa tgaggagatt
     7501 aagttcggtt tacgtttatt ttagtactaa ttgtaacgtg agactacgta tcgggaatcg
     7561 cctaggatcc aggatgattt ctggtttaaa caagcttcgg ctggatctta tgacgaccgt
     7621 tagaaattgt ggttgtcgac gagtcagtaa taaacggcgt caaagtggtt gcagccggca
     7681 cacacgagtc gtgtttatca actcaaagca caaatacttt tcctcaacct aaaaataagg
     7741 caattagcca aaaacaactt tgcgtgtaaa caacgctcaa tacacgtgtc attttattat
     7801 tagctattgc ttcaccgcct tagctttctc gtgacctagt cgtcctcgtc ttttcttctt
     7861 cttcttctat aaaacaatac ccaaagagct cttcttcttc acaattcaga tttcaatttc
     7921 tcaaaatctt aaaaactttc tctcaattct ctctaccgtg atcaaggtaa atttctgtgt
     7981 tccttattct ctcaaaatct tcgattttgt tttcgttcga tcccaatttc gtatatgttc
     8041 tttggtttag attctgttaa tcttagatcg aagacgattt tctgggtttg atcgttagat
     8101 atcatcttaa ttctcgatta gggtttcata gatatcatcc gatttgttca aataatttga
     8161 gttttgtcga ataattactc ttcgatttgt gatttctatc tagatctggt gttagtttct
     8221 agtttgtgcg atcgaatttg tcgattaatc tgagtttttc tgattaacag atgctagcct
     8281 ccaaaaccat cgttctttcg gtcggcgagg ctactcgcac tctgactgag atccagtcca
     8341 ccgcagaccg tcagatcttc gaagagaagg tcgggcctct ggtgggtcgg ctgcgcctca
     8401 cggcttcgct ccgtcaaaac ggagccaaga ccgcgtatcg cgtcaaccta aaactggatc
     8461 aggcggacgt cgttgattcc ggacttccga aagtgcgcta cactcaggta tggtcgcacg
     8521 acgtgacaat cgttgcgaat agcaccgagg cctcgcgcaa atcgttgtac gatttgacca
     8581 agtccctcgt cgcgacctcg caggtcgaag atcttgtcgt caaccttgtg ccgctgggcc
     8641 gtggatccat ggtagatctg actagtaaag gagaagaact tttcactgga gttgtcccaa
     8701 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
     8761 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
     8821 ctgttccgtg gccaacactt gtcactactt tctcttatgg tgttcaatgc ttttcaagat
     8881 acccagatca tatgaagcgg cacgacttct tcaagagcgc catgcctgag ggatacgtgc
     8941 aggagaggac catcttcttc aaggacgacg ggaactacaa gacacgtgct gaagtcaagt
     9001 ttgagggaga caccctcgtc aacaggatcg agcttaaggg aatcgatttc aaggaggacg
     9061 gaaacatcct cggccacaag ttggaataca actacaactc ccacaacgta tacatcatgg
     9121 ccgacaagca aaagaacggc atcaaagcca acttcaagac ccgccacaac atcgaagacg
     9181 gcggcgtgca actcgctgat cattatcaac aaaatactcc aattggcgat ggccctgtcc
     9241 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
     9301 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
     9361 atgaactata caaagctagc ccatggtaag ctttttgtga tctgatgata agtggttggt
     9421 tcgtgtctca tgcacttggg aggtgatcta tttcacctgg tgtagtttgt gtttccgtca
     9481 gttggaaaaa cttatcccta tcgatttcgt tttcattttc tgcttttctt ttatgtacct
     9541 tcgtttgggc ttgtaacggg cctttgtatt tcaactctca ataataatcc aagtgcatgt
     9601 taaacaattt gtcatctgtt tcggctttga tatactactg gtgaagatgg gccgtactac
     9661 tgcatcacaa cgaaaaataa taataagatg aaaaacttga agtggaaaaa aaaaaaactt
     9721 gaatgttcac tactactcat tgaccataat gtttaacata catagctcaa tagtattttt
     9781 gtgaatatgg caacacaaac agtccaaaac aattgtctct tactatacca aaccaagggc
     9841 gccgcttgtt tgccactctt tgtgtgcaat agtgtgatta ccacatctcc acattcaata
     9901 tattccctga attatctgac gattttgatg gctcactgtt ttcccaagtc tggtacccca
     9961 cctacatggg gatcctaact cgagctgcag gcttcggtac cgagctcgaa ttcgtaatca
    10021 tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    10081 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    10141 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    10201 tcggccaacg cgcggggaga ggcggtttgc gtattggcta gagcagcttg ccaacatggt
    10261 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag
    10321 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
    10381 agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca
    10441 tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga
    10501 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
    10561 gcaagtggat tgatgtgaac atggtggagc acgacactct cgtctactcc aagaatatca
    10621 aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg
    10681 gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa
    10741 aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
    10801 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
    10861 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
    10921 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
    10981 ttcatttgga gaggacacgc tgaaatcacc agtctctctc tacaaatcta tctctctcga
    11041 gctttcgcag atccgggggg caatgagata tgaaaaagcc tgaactcacc gcgacgtctg
    11101 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
    11161 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
    11221 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
    11281 cgctcccgat tccggaagtg cttgacattg gggagtttag cgagagcctg acctattgca
    11341 tctcccgccg ttcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
    11401 ttctacaacc ggtcgcggag gctatggatg cgatcgctgc ggccgatctt agccagacga
    11461 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
    11521 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
    11581 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
    11641 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
    11701 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
    11761 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
    11821 ggaggcatcc ggagcttgca ggatcgccac gactccgggc gtatatgctc cgcattggtc
    11881 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
    11941 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
    12001 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
    12061 gacgccccag cactcgtccg agggcaaaga aatagagtag atgccgaccg ggatctgtcg
    12121 atcgacaagc tcgagtttct ccataataat gtgtgagtag ttcccagata agggaattag
    12181 ggttcctata gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt
    12241 atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa
    12301 aatccagatc ccccgaatta attcggcgtt aattcagtac attaaaaacg tccgcaatgt
    12361 gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag
    12421 ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc
    12481 agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc
    12541 gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg
    12601 agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca ccgcggtttc
    12661 aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct
    12721 gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta
    12781 taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg
    12841 ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag
    12901 atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat
    12961 atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg
    13021 acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc
    13081 atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt
    13141 atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct
    13201 ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg
    13261 aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag
    13321 acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag
    13381 aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca
    13441 aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg
    13501 ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt
    13561 ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg
    13621 aattgtttta gtacctagaa tgcatgacca aaatccctta acgtgagttt tcgttccact
    13681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    13741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    13801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    13861 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    13921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    13981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    14041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    14101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    14161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    14221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    14281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    14341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    14401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    14461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
    14521 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
    14581 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
    14641 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
    14701 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
    14761 acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt
    14821 gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg
    14881 cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt
    14941 tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa
    15001 gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat
    15061 cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt
    15121 tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac
    15181 cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg
    15241 aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag
    15301 cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc
    15361 gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct
    15421 tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac
    15481 ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct
    15541 tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc
    15601 gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg
    15661 tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta
    15721 accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga
    15781 acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt
    15841 cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat
    15901 cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct
    15961 cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt
    16021 ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg
    16081 gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc
    16141 gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg
    16201 cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac
    16261 ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc
    16321 gggcttgggg gttccagtgc cattgcaggg ccggcaggca acccagccgc ttacgcctgg
    16381 ccaaccgccc gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg
    16441 attttccatg ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca
    16501 tttactctgg tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc
    16561 gtaccgcgta catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct
    16621 tcatggctgg cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg
    16681 gacggccggc acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca
    16741 aatgagtttt gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc
    16801 gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg
    16861 cgtgatacgg gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc
    16921 gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc
    16981 cgtgacctca atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta
    17041 agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa
    17101 gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac
    17161 gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac
    17221 ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc
    17281 ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt
    17341 ctggttaagt acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat
    17401 cgcatcatat acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt
    17461 tttagacggc ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg
    17521 gcatcagaca aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc
    17581 tcgaacacgt acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt
    17641 tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg
    17701 ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct
    17761 cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc
    17821 caagcagtgc agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt
    17881 gcgcgatctg tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg
    17941 cggcctcgcg cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc
    18001 cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg
    18061 ccaggctacg caggcccgcg ccggcctcct ggatgcg
//
