LOCUS       sequence-319171-        8388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 Spike Protein for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman 
            J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter 
            G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt 
            AG, Iafrate AJ, Balazs AB
  TITLE     COVID-19-neutralizing antibodies predict disease severity and 
            survival.
  JOURNAL   Cell. 2021 Jan 21;184(2):476-488.e11. doi: 
            10.1016/j.cell.2020.12.015. Epub 2020 Dec 15.
  PUBMED    33412089
REFERENCE   2  (bases 1 to 8388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.015"; journalName: "Cell"; date: 
            "2021-01-21- 21"; volume: "184"; issue: "2"; pages: "476-488.e11"
COMMENT     Sequence Label: pHDM-SARS-CoV-2
FEATURES             Location/Qualifiers
     source          1..8388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        82..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     662..686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          779..1254
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     complement(5160..5176)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5161..5177)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    5270..5664
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(5998..6016)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6084..6188
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6189..6257,6258..7049)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6407..6426)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7220..7808
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7709..7728
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcttggccc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat
       61 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
      121 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgggaccga tccagcctcc cctcgaagct gatcctgaga acttcagggt
      781 gagtctatgg gacccttgat gttttctttc cccttctttt ctatggttaa gttcatgtca
      841 taggaagggg agaagtaaca gggtacacat attgaccaaa tcagggtaat tttgcatttg
      901 taattttaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
      961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
     1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
     1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
     1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
     1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
     1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattccg cgggcggccg
     1321 ccatgttcgt gttcctggtg ctgctgcctc tggtgtccag ccagtgtgtg aacctgacca
     1381 ccagaacaca gctgcctcca gcctacacca acagctttac cagaggcgtg tactaccccg
     1441 acaaggtgtt cagatccagc gtgctgcact ctacccagga cctgttcctg cctttcttca
     1501 gcaacgtgac ctggttccac gccatccacg tgtccggcac caatggcacc aagagattcg
     1561 acaaccccgt gctgcccttc aacgacgggg tgtactttgc cagcaccgag aagtccaaca
     1621 tcatcagagg ctggatcttc ggcaccacac tggacagcaa gacccagagc ctgctgatcg
     1681 tgaacaacgc caccaacgtg gtcatcaaag tgtgcgagtt ccagttctgc aacgacccct
     1741 tcctgggcgt ctactaccac aagaacaaca agagctggat ggaaagcgag ttccgggtgt
     1801 acagcagcgc caacaactgc accttcgagt acgtgtccca gcctttcctg atggacctgg
     1861 aaggcaagca gggcaacttc aagaacctgc gcgagttcgt gtttaagaac atcgacggct
     1921 acttcaaaat ctacagcaag cacaccccta tcaacctcgt gcgggatctg cctcagggct
     1981 tctctgctct ggaacccctg gtggatctgc ccatcggcat caacatcacc cggtttcaga
     2041 cactgctggc cctgcacaga agctacctga cacctggcga tagcagcagc ggatggacag
     2101 ctggtgccgc cgcttactat gtgggctacc tgcagcctag aaccttcctg ctgaagtaca
     2161 acgagaacgg caccatcacc gacgccgtgg attgtgctct ggaccctctg agcgagacaa
     2221 agtgcaccct gaagtccttc accgtggaaa agggcatcta ccagaccagc aacttccggg
     2281 tgcagcccac cgaatccatc gtgcggttcc ccaatatcac caatctgtgc cccttcggcg
     2341 aggtgttcaa tgccaccaga ttcgcctctg tgtacgcctg gaaccggaag cggatcagca
     2401 attgcgtggc cgactactcc gtgctgtaca actccgccag cttcagcacc ttcaagtgct
     2461 acggcgtgtc ccctaccaag ctgaacgacc tgtgcttcac aaacgtgtac gccgacagct
     2521 tcgtgatccg gggagatgaa gtgcggcaga ttgcccctgg acagacaggc aagatcgccg
     2581 actacaacta caagctgccc gacgacttca ccggctgtgt gattgcctgg aacagcaaca
     2641 acctggactc caaagtcggc ggcaactaca attacctgta ccggctgttc cggaagtcca
     2701 atctgaagcc cttcgagcgg gacatcagca ccgaaatcta tcaggccggc agcacccctt
     2761 gcaacggcgt ggaaggcttc aactgctact tcccactgca gtcctacggc tttcagccca
     2821 caaatggcgt gggctatcag ccctacagag tggtggtgct gagcttcgaa ctgctgcatg
     2881 cccctgccac agtgtgcggc cctaagaaaa gcaccaatct cgtgaagaac aaatgcgtga
     2941 acttcaactt caacggcctg accggcaccg gcgtgctgac agagagcaac aagaagttcc
     3001 tgccattcca gcagtttggc cgggatatcg ccgataccac agacgccgtt agagatcccc
     3061 agacactgga aatcctggac atcaccccat gcagcttcgg cggagtgtct gtgatcaccc
     3121 ctggcaccaa caccagcaat caggtggcag tgctgtacca ggacgtgaac tgtaccgaag
     3181 tgcccgtggc cattcacgcc gatcagctga cacctacatg gcgggtgtac tccaccggca
     3241 gcaatgtgtt tcagaccaga gccggctgtc tgatcggagc cgagcacgtg aacaatagct
     3301 acgagtgcga catccccatc ggcgctggca tctgtgccag ctaccagaca cagacaaaca
     3361 gccccagacg ggctagaagc gtggccagcc agagcatcat tgcctacaca atgtctctgg
     3421 gcgccgagaa cagcgtggcc tactccaaca actctatcgc tatccccacc aacttcacca
     3481 tcagcgtgac caccgagatc ctgcctgtgt ccatgaccaa gaccagcgtg gactgcacca
     3541 tgtacatctg cggcgattcc accgagtgct ccaacctgct gctgcagtac ggcagcttct
     3601 gcacccagct gaatagagcc ctgacaggga tcgccgtgga acaggacaag aacacccaag
     3661 aggtgttcgc ccaagtgaag caaatctaca agacccctcc tatcaaggac ttcggcggct
     3721 tcaatttcag ccagattctg cccgatccta gcaagcccag caagcggagc ttcatcgagg
     3781 acctgctgtt caacaaagtg acactggccg acgccggctt catcaagcag tatggcgatt
     3841 gtctgggcga cattgccgcc agggatctga tttgcgccca gaagtttaac ggactgacag
     3901 tgctgcctcc tctgctgacc gatgagatga tcgcccagta cacatctgcc ctgctggccg
     3961 gcacaatcac aagcggctgg acatttggag ctggcgccgc tctgcagatc ccctttgcta
     4021 tgcagatggc ctaccggttc aacggcatcg gagtgaccca gaatgtgctg tacgagaacc
     4081 agaagctgat cgccaaccag ttcaacagcg ccatcggcaa gatccaggac agcctgagca
     4141 gcacagcaag cgccctggga aagctgcagg acgtggtcaa ccagaatgcc caggcactga
     4201 acaccctggt caagcagctg tcctccaact tcggcgccat cagctctgtg ctgaacgata
     4261 tcctgagcag actggacaag gtggaagccg aggtgcagat cgacagactg atcaccggaa
     4321 ggctgcagtc cctgcagacc tacgttaccc agcagctgat cagagccgcc gagattagag
     4381 cctctgccaa tctggccgcc accaagatgt ctgagtgtgt gctgggccag agcaagagag
     4441 tggacttttg cggcaagggc taccacctga tgagcttccc tcagtctgcc cctcacggcg
     4501 tggtgtttct gcacgtgaca tacgtgcccg ctcaagagaa gaatttcacc accgctccag
     4561 ccatctgcca cgacggcaaa gcccactttc ctagagaagg cgtgttcgtg tccaacggca
     4621 cccattggtt cgtgacccag cggaacttct acgagcccca gatcatcacc accgacaaca
     4681 ccttcgtgtc tggcaactgc gacgtcgtga tcggcattgt gaacaatacc gtgtacgacc
     4741 ctctgcagcc cgagctggac agcttcaaag aggaactgga taagtacttt aagaaccaca
     4801 caagccccga cgtggacctg ggcgatatca gcggaatcaa tgccagcgtc gtgaacatcc
     4861 agaaagagat cgaccggctg aacgaggtgg ccaagaatct gaacgagagc ctgatcgacc
     4921 tgcaagaact ggggaagtac gagcagtaca tcaagtggcc ctggtacatc tggctgggct
     4981 ttatcgccgg actgattgcc atcgtgatgg tcacaatcat gctgtgttgc atgaccagct
     5041 gctgtagctg cctgaagggc tgttgctctt gcggctcttg ctgcaaattc gatgaggacg
     5101 attccgaacc tgtcttgaag ggtgtcaagc tccattacac ataaaggatc caagcttatc
     5161 gataccgtcg acctcgaggg cccagatcta attcacccca ccagtgcagg ctgcctatca
     5221 gaaagtggtg gctggtgtgg ctaatgccct ggcccacaag tatcactaag ctcgctttct
     5281 tgctgtccaa tttctattaa aggttccttt gttccctaag tccaactact aaactggggg
     5341 atattatgaa gggccttgag catctggatt ctgcctaata aaaaacattt attttcattg
     5401 caatgatgta tttaaattat ttctgaatat tttactaaaa agggaatgtg ggaggtcagt
     5461 gcatttaaaa cataaagaaa tgaagagcta gttcaaacct tgggaaaata cactatatct
     5521 taaactccat gaaagaaggt gaggctgcaa acagctaatg cacattggca acagcccctg
     5581 atgcctatgc cttattcatc cctcagaaaa ggattcaagt agaggcttga tttggaggtt
     5641 aaagttttgc tatgctgtat tttacattac ttattgtttt agctgtcctc atgaatgtct
     5701 tttcactacc catttgctta tcctgcatct ctcagccttg actccactca gttctcttgc
     5761 ttagagatac cacctttccc ctgaagtgtt ccttccatgt tttacggcga gatggtttct
     5821 cctcgcctgg ccactcagcc ttagttgtct ctgttgtctt atagaggtct acttgaagaa
     5881 ggaaaaacag ggggcatggt ttgactgtcc tgtgagccct tcttccctgc ctcccccact
     5941 cacagtgacc cggaatccct cgacatggca gtctagatca ttcttgaaga cgaaagggcc
     6001 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     6061 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6121 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6181 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6241 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6301 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6361 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6421 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6481 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6541 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6601 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6661 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6721 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6781 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6841 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6901 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6961 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7021 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7081 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7141 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7201 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7261 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7321 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7381 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7441 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7501 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7561 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7621 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7681 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7741 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7801 tatggaaaaa cgccagcaac ggatgcgccg cgtgcggctg ctggagatgg cggacgcgat
     7861 ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc
     7921 tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag
     7981 gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg
     8041 cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat ccccgtgacg
     8101 atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt
     8161 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg
     8221 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cggggagctt
     8281 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     8341 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatg
//
