LOCUS       sequence-319176-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codon-optimized bacterial expression plasmid. Expresses 
            GB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Elias RD, Ramaraju B, Deshmukh L
  TITLE     Mechanistic Roles of Tyrosine Phosphorylation in Reversible 
            Amyloids, Autoinhibition, and Endosomal Membrane Association of 
            ALIX.
  JOURNAL   J Biol Chem. 2021 Oct 21:101328. doi: 10.1016/j.jbc.2021.101328.
  PUBMED    34688656
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2021 Oct 21:101328. doi: 10.1016/j.jbc.2021.101328."
COMMENT     Sequence Label: GB1-PRD(703-868, A756C, P801G, C813S)-Strep
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             166..183
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             184..195
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             235..252
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             253..273
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             772..795
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 caatacaaac tggcgctgaa cggcaaaacg ctgaagggtg aaacgacgac cgaggccgtt
       61 gatgcggcca cggccgagaa agtgttcaag cagtacgcca acgacaacgg tgttgatggc
      121 gaatggacct acgacgatgc gaccaaaacc ttcaccgtga ccgaactggt tccgcgcggc
      181 agtatcgaag gtcgcatccg cgagaatctg aaagatagcg gtctgtttct ggagcatcac
      241 caccatcacc atgaaaatct gtacttccaa ggcgacgaac tgctgaaaga tctgcagcag
      301 agcattgccc gcgaaccaag cgccccaagc atcccgacgc cagcctatca gagtagtccg
      361 gcgggtggtc atgcgccgac cccaccaacg ccggccccac gtacgatgcc gccaaccaaa
      421 ccgcaaccac catgtcgtcc gccaccacca gttctcccag ccaatcgcgc cccaagtgcg
      481 accgcgccaa gcccagttgg tgcgggtacc gccgcgccag cgccaagtca aacgccgggt
      541 agtgccccac caccacaagc gcaaggcggc ccatatccga cgtacccggg ctatccgggc
      601 tacagtcaga tgccaatgcc gatgggttac aacccatacg cgtacggcca gtataacatg
      661 ccgtacccac cggtgtacca ccagagtccg ggccaagccc catacccggg tccacagcag
      721 ccaagctacc cgttcccgca gccaccacag caaagttact acccgcagca atggagccat
      781 ccgcagttcg aaaagtaa
//
