LOCUS       sequence-319219-       10954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAVS1 targeting donor plasmid with mCherry expression cassette and 
            puromycin selection gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10954)
  AUTHORS   Xiong M, Tao Y, Gao Q, Feng B, Yan W, Zhou Y, Kotsonis TA, Yuan T, 
            You Z, Wu Z, Xi J, Haberman A, Graham J, Block J, Zhou W, Chen Y, 
            Zhang SC
  TITLE     Human Stem Cell-Derived Neurons Repair Circuits and Restore Neural 
            Function.
  JOURNAL   Cell Stem Cell. 2020 Sep 16. pii: S1934-5909(20)30410-0. doi: 
            10.1016/j.stem.2020.08.014.
  PUBMED    32966778
REFERENCE   2  (bases 1 to 10954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2020 Sep 16. pii: S1934-5909(20)30410-0. doi: 
            10.1016/j.stem.2020.08.014."
COMMENT     Sequence Label: AAVS1-pur-CAG-mCherry
FEATURES             Location/Qualifiers
     source          1..10954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1740..1749
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1746..2456
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1891..1909)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2159..2178
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2490..3078
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2543..2563)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2961..2972)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3110..3586
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3309..3328)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3696..3715)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3761..3816
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3815..3834)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4177..4193)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4201..4217
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4225..4231,4232..4249,4250..4255))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4270..4291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    4365..5201
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(5235..5254)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5236..5254)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5261..5278)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5261..5277)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5270..5292)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             5415..5717
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5596..5615
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(6120..6139)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6734..6753
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(7114..7905,7906..7974))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7737..7756
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8098..8686
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8587..8606
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8840..8857
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8974..8995
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9010..9015,9016..9033,9034..9040)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9048..9064
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9072..9088
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9109..9127
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    9166..9969
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    9976..10001
                     /label=SA
                     /note="splice acceptor site"
     CDS             10025..10078
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             10088..10687
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10088..10107)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10584..10604
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(10719..10736)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10725..10949
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc crggsggggs ggggsggggs grggggsggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggrg ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc gtcgacgccg
     1741 ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
     1801 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     1861 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     1921 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     1981 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     2041 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     2101 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     2161 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     2221 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     2281 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     2341 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     2401 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaataga
     2461 cgcgtgaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     2521 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     2581 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     2641 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     2701 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     2761 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     2821 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     2881 ggaaatcatc gtcctttcct tggctgctcg cctatgttgc cacctggatt ctgcgcggga
     2941 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     3001 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     3061 tttgggccgc ctccccgcat cgataccgag cgctgctcga gagatctacg ggtggcatcc
     3121 ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag
     3181 ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat
     3241 tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc
     3301 ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat
     3361 ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca
     3421 ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat
     3481 attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa
     3541 attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttgtaggt
     3601 aaccaggcgc gtgaattcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     3661 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
     3721 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     3781 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     3841 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     3901 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     3961 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     4021 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     4081 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     4141 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     4201 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     4261 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     4321 gtcgggaaac ctgtcgtgcc agcggatcga cagtactaag ctttactagg gacaggattg
     4381 gtgacagaaa agccccatcc ttaggcctcc tccttcctag tctcctgata ttgggtctaa
     4441 cccccacctc ctgttaggca gattccttat ctggtgacac acccccattt cctggagcca
     4501 tctctctcct tgccagaacc tctaaggttt gcttacgatg gagccagaga ggatcctggg
     4561 agggagagct tggcaggggg tgggagggaa gggggggatg cgtgacctgc ccggttctca
     4621 gtggccaccc tgcgctaccc tctcccagaa cctgagctgc tctgacgcgg ctgtctggtg
     4681 cgtttcactg atcctggtgc tgcagcttcc ttacacttcc caagaggaga agcagtttgg
     4741 aaaaacaaaa tcagaataag ttggtcctga gttctaactt tggctcttca cctttctagt
     4801 ccccaattta tattgttcct ccgtgcgtca gttttacctg tgagataagg ccagtagcca
     4861 gccccgtcct ggcagggctg tggtgaggag gggggtgtcc gtgtggaaaa ctccctttgt
     4921 gagaatggtg cgtcctaggt gttcaccagg tcgtggccgc ctctactccc tttctctttc
     4981 tccatccttc tttccttaaa gagtccccag tgctatctgg gacatattcc tccgcccaga
     5041 gcagggtccc gcttccctaa ggccctgctc tgggcttctg ggtttgagtc cttggcaagc
     5101 ccaggagagg cgctcaggct tccctgtccc ccttcctcgt ccaccatctc atgcccctgg
     5161 ctctcctgcc ccttccctac aggggttcct ggctctgctc taagggcgaa ttcgcggccg
     5221 ctaaattcaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
     5281 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     5341 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     5401 gcctatacgt acggcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt
     5461 ttgtggatgt acagagtgat attattgaca cgccggggcg acggatggtg atccccctgg
     5521 ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg
     5581 gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg
     5641 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga
     5701 tgttctgggg aatataaatg tcaggcatga gattatcaaa aaggatcttc acctagatcc
     5761 ttttcacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact
     5821 gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc
     5881 ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag
     5941 ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc
     6001 cgccaaggat ctgatggcgc aggggatcaa gctctgatca agagacagga tgaggatcgt
     6061 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     6121 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     6181 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     6241 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     6301 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     6361 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     6421 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     6481 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     6541 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     6601 ccgacggcga ggatctcgtc gtgacccatg catggcgatg cctgcttgcc gaatatcatg
     6661 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     6721 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     6781 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     6841 cgccttcttg acgagttctt ctgaattatt aacgcttaca atttcctgat gcggtatttt
     6901 ctccttacgc atctgtgcgg tatttcacac cgcatcaggt ggcacttttc ggggaaatgt
     6961 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7021 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7081 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7141 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7201 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7261 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7321 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7381 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7441 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7501 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7561 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7621 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgaatact caaccaagtc
     7681 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7741 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7801 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7861 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7921 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7981 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgacca aaatccctta
     8041 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8101 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8161 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8221 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8281 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8341 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8401 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8461 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8521 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8581 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8641 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8701 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8761 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8821 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8881 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8941 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     9001 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     9061 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctcagaa ttaaccctca
     9121 ctaaagggac tagctagtcc tgcaggttta aacgaattcg ccctttgctt tctctgacca
     9181 gcattctctc ccctgggcct gtgccgcttt ctgtctgcag cttgtggcct gggtcacctc
     9241 tacggctggc ccagatcctt ccctgccgcc tccttcaggt tccgtcttcc tccactccct
     9301 cttccccttg ctctctgctg tgttgctgcc caaggatgct ctttccggag cacttccttc
     9361 tcggcgctgc accacgtgat gtcctctgag cggatcctcc ccgtgtctgg gtcctctccg
     9421 ggcatctctc ctccctcacc caaccccatg ccgtcttcac tcgctgggtt cccttttcct
     9481 tctccttctg gggcctgtgc catctctcgt ttcttaggat ggccttctcc gacggatgtc
     9541 tcccttgcgt cccgcctccc cttcttgtag gcctgcatca tcaccgtttt tctggacaac
     9601 cccaaagtac cccgtctccc tggctttagc cacctctcca tcctcttgct ttctttgcct
     9661 ggacaccccg ttctcctgtg gattcgggtc acctctcact cctttcattt gggcagctcc
     9721 cctacccccc ttacctctct agtctgtgct agctcttcca gccccctgtc atggcatctt
     9781 ccaggggtcc gagagctcag ctagtcttct tcctccaacc cgggccccta tgtccacttc
     9841 aggacagcat gtttgctgcc tccagggatc ctgtgtcccc gagctgggac caccttatat
     9901 tcccagggcc ggttaatgtg gctctggttc tgggtacttt tatctgtccc ctccacccca
     9961 cagtggggca agcttctgac ctcttctctt cctcccacag ggcctcgaga gatctggcag
    10021 cggagagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctag
    10081 gctcgagatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
    10141 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
    10201 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
    10261 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
    10321 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
    10381 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
    10441 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
    10501 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
    10561 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
    10621 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
    10681 tgcctgatct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
    10741 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
    10801 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
    10861 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
    10921 gcatgctggg gatgcggtgg gctctatggg tctc
//
