LOCUS       sequence-319232-          20 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sgRNA targeting human GJA1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 20)
  AUTHORS   Cheng L, Cring MR, Wadkins DA, Kuehn MH
  TITLE     Absence of Connexin 43 results in smaller retinas and arrested, 
            depolarized retinal progenitor cells in human retinal organoids.
  JOURNAL   Stem Cells. 2022 Mar 9. pii: 6545973. doi: 10.1093/stmcls/sxac017.
  PUBMED    35263762
REFERENCE   2  (bases 1 to 20)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2022 Mar 9. pii: 6545973. doi: 10.1093/stmcls/sxac017."
COMMENT     Sequence Label: pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA
FEATURES             Location/Qualifiers
     source          1..20
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aagcctactc aactgctgga
//
