LOCUS       sequence-319261-        9387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express ABE7.10-P2A-GFP by hPGK promoter in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Shin HR, See JE, Kweon J, Kim HS, Sung GJ, Park S, Jang AH, Jang G, 
            Choi KC, Kim I, Kim JS, Kim Y
  TITLE     Small-molecule inhibitors of histone deacetylase improve 
            CRISPR-based adenine base editing.
  JOURNAL   Nucleic Acids Res. 2021 Feb 26;49(4):2390-2399. doi: 
            10.1093/nar/gkab052.
  PUBMED    33544854
REFERENCE   2  (bases 1 to 9387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2021-02-26- 26"; volume: "49"; issue: 
            "4"; pages: "2390-2399"
COMMENT     Sequence Label: hPGK-ABE7.10-P2A-GFP
FEATURES             Location/Qualifiers
     source          1..9387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1118..1137)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1119..1137)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1144..1654)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1207..1225)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     1820..1839
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1918..2022
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2023..2091,2092..2883)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2241..2260)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3054..3642
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3543..3562
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3796..3813
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3930..3951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3966..3971,3972..3989,3990..3996)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4004..4020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4009..4031
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4028..4044
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4028..4044
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    4115..4339
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4328..4345
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(4368..4385)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(4394..4414)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(4427..5137,5138..5140,5141..5143))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4427..5137,5138..5140,5141..5143))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4469..4490)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4818..4837
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5077..5098
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(5144..5200)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(join(5201..5293,5294..5296,5297..5301,5302,
                     5303..5899,5900..5902,5903..9301))
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(join(5201..9274,9275..9277,9278..9301))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(join(9302..9387,1..10,11..508,509..604,
                     605..1105))
                     /codon_start=1
                     /product="adenine base editor comprising wild-type E. coli 
                     TadA fused to the evolved TadA*(7.10) mutant (Gaudelli et 
                     al., 2017)"
                     /label=ABE(7.10)
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGW
                     NRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRVVF
                     GARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKKAQ
                     SSTDSGGSSGGSSGSETPGTSESATPESSGGSSGGSSEVEFSHEYWMRHALTLAKRARD
                     EREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVT
                     FEPCVMCAGAMIHSRIGRVVFGVRNAKTGAAGSLMDVLHYPGMNHRVEITEGILADECA
                     ALLCYFFRMPRQVFNAQKKAQSSTDSGGSSGGSSGSETPGTSESATPESSGGSSGGS"
ORIGIN
        1 aaccaccaga gtcagtagag gattgtgctt ttttctgggc gttaaagacc tgcctgggca
       61 tgcgaaaaaa gtaacacaac agcgccgcac attcgtccgc caatatgcct tctgtgattt
      121 ctacccggtg gttcatgcct gggtaatgca gcacgtccat cagtgaacct gcggcacccg
      181 tcttggcgtt gcgaacaccg aatacaactc gtccaatgcg ggagtgaatc atagctcccg
      241 cgcacattac gcaaggttca aacgtgacgt acagcgtcgc atcgataagt cgataattct
      301 gcatcacaag ccctccctgt cgaagggcca tgatttccgc atgtgcagtg gggtcgtgga
      361 gtccgattgc cctattccaa ccttcgccga ttacgcgatt gttgagcacg agtactgccc
      421 ccacgggcac ctcgcgttca tctcgagccc tctttgcgag agtcaatgcg tgtctcatcc
      481 agtactcatg ggaaaactcg acttcggaag aaccaccaga agaaccacca gaactttcgg
      541 gtgtggcgga ctctgaggtc ccgggagtct cgctgccgct agaaccacca gaagaaccac
      601 cagagtcagt agaggattgt gcttttttct gggccttgat ctcctgcctc cgcatgcgaa
      661 aaaagtcgga caacagcgcc gcacattcgt ccgccaatat gccttctgtg atttctaccc
      721 ggtggttcat gcctgggtga tgcagcacgt ccatcagtga acctgcggca cccgtcttgg
      781 cgtcgcgggc accgaataca actcgtccaa tgcgggagtg aatcatagct cccgcgcaca
      841 ttacgcaagg ttcaagcgtg acgtacagcg tcgcatcgat aagtcgataa ttctgcatca
      901 caagccctcc ctgtcgaagg gccatgattt ccgcatgtgc agtggggtcg tggcgtccga
      961 tcggcctatt ccaaccttcg ccgattacgc gattgttatg cacgagtact gcccccacgg
     1021 gcacctcgcg ttcatcccaa gccctctttg cgagagtcaa tgcgtgtctc atccagtact
     1081 catgggaaaa ctcgacttcg gacatggtgg cggctctccc tatagtgagt cgtattagcg
     1141 gccctgggga gagaggtcgg tgattcggtc aacgagggag ccgactgccg acgtgcgctc
     1201 cggaggcttg cagaatgcgg aacaccgcgc gggcaggaac agggcccaca ctaccgcccc
     1261 acaccccgcc tcccgcaccg ccccttcccg gccgctgctc tcggcgcgcc ctgctgagca
     1321 gccgctattg gccacagccc atcgcggtcg gcgcgctgcc attgctccct ggcgctgtcc
     1381 gtctgcgagg gtactagtga gacgtgcggc ttccgtttgt cacgtccggc acgccgcgaa
     1441 ccgcaaggaa ccttcccgac ttaggggcgg agcaggaagc gtcgccgggg ggcccacaag
     1501 ggtagcggcg aagatccggg tgacgctgcg aacggacgtg aagaatgtgc gagacccagg
     1561 gtcggcgccg ctgcgtttcc cggaaccacg cccagagcag ccgcgtccct gcgcaaaccc
     1621 agggctgcct tggaaaaggc gcaaccccaa cccctatatc tggcccgtac atcgcgaagc
     1681 agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt
     1741 gttgtagctt aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc
     1801 agatactggc ttaactatgc ggcatcagag cagattgtac tgagagtcga ccctagggga
     1861 tcgggagatc gatctcccga tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc
     1921 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1981 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2041 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2101 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     2161 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     2221 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     2281 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     2341 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     2401 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     2461 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2521 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2581 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2641 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2701 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2761 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2821 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2881 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2941 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3001 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3061 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3121 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3181 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3241 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3301 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3361 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3421 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3481 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3541 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3601 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3661 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3721 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3781 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3841 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3901 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3961 taggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     4021 tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgacggt
     4081 atcgagcccc agctggttct ttccgcctca gaagccatag agcccaccgc atccccagca
     4141 tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga
     4201 atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca
     4261 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
     4321 tggcaactag aaggcacagt cgaggctgat cagcgggttt aaactcaatg gtgatggtga
     4381 tgatgaccgg ttagactttc ctcttcttct tgggagaacc accagacttg tacagctcgt
     4441 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
     4501 tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca
     4561 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc
     4621 cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg
     4681 ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc
     4741 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga
     4801 acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct
     4861 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
     4921 agcggctgaa gcactgcact ccataggtca gggtggtcac gagggtgggc cagggcacgg
     4981 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
     5041 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
     5101 tgggcaccac cccggtgaac agctcctcgc ccttgctcac cataggtcca gggttctcct
     5161 ccacgtctcc agcctgcttc agcaggctga agttagtagc gtcaccccca agctgtgaca
     5221 aatctatccg agtttcatat aatcccgtga tggattggtg aatcagtgtc gcgtctagca
     5281 cctccttggt agaagtgtat cgtttgcgat ctatcgttgt gtcaaaatac ttgaatgcgg
     5341 ctggagcgcc gaggttggta agagtaaaca aatggataat attttccgcc tgctcacgta
     5401 tgggtttatc cctgtgcttg ttgtatgcgc ttaatacttt gtccagattg gcatcagcta
     5461 ggatgactct cttactgaat tccgaaattt gctctatgat ttcgtcgaga taatgtttgt
     5521 gctgctcaac aaaaagttgc ttctgttcgt tatcttcagg tgaacctttc aacttctcgt
     5581 aatgggacgc taaatacagg aaattcacgt atttagacgg tagtgcgagt tcgttcccct
     5641 tttgaagctc tccggcgcta gccaacatcc gttttcggcc attttctaac tcaaacagac
     5701 tatactttgg tagtttaatt atgagatcct tttttacttc cttgtaacct ttcgcctcaa
     5761 ggaagtcgat ggggttcttt tcaaaagacg agcgctccat aatcgttatc cccaataatt
     5821 ctttgactga cttcagtttc ttggattttc ccttctcaac ttttgccact actaggacag
     5881 aataggcaac tgtagggcta tcgaagccac cgtacttttt cgggtcccag tcctttttac
     5941 gagcgatgag cttatcacta ttcctttttg gaagaatcga ttcctttgaa aaccctccgg
     6001 tctgcacctc agttttcttt actatgttga cttggggcat ggacaaaact tttctcaccg
     6061 tcgcgaagtc ccggccctta tcccatacga tttcacctgt ctccccattg gtttcaatta
     6121 aaggtcgttt gcgtatctct ccgtttgcca gagtgatttc cgtcttaaag aaattcataa
     6181 tgttagaata aaagaagtat ttggctgtag ccttgcctat ctcctgttcg cttttcgcga
     6241 tcatcttacg gacgtcataa actttgtaat caccatacac aaactcactt tctagcttcg
     6301 ggtatttctt aatgagtgcg gtccctacga cggcattaag ataagcgtcg tgcgcatggt
     6361 ggtagttatt tatctcccta actttataga attgaaaatc ctttctgaag tccgacacca
     6421 attttgactt taaagtgatt actttgactt cccgaatcag cttatcgttc tcgtcgtatt
     6481 tcgtattcat tcgggaatct agtatctgtg caacatgctt tgtgatttgg cgggtttcca
     6541 cgagctgacg tttaataaat ccggccttgt caagttcaga caagccaccc ctctcagctt
     6601 tagttaagtt atcgaacttt ctttgcgtta tcagtttcgc atttaggagc tgccgccaat
     6661 agttcttcat tttctttacg acttcctcgc ttggaacatt gtcacttttc cctcggttct
     6721 tatccgagcg tgtaagcact ttattgtcga ttgaatcgtc cttcaaaaag gattggggta
     6781 caatgtgatc gacgtcgtaa tcagataaac ggtttatgtc cagttcctga tcaacataca
     6841 tgtcccttcc attttgtagg taatagaggt aaagtttctc gttctgcaat tgggtatttt
     6901 ccacaggatg ctcctttaag atctggctgc ccagttcttt aataccctct tctattctct
     6961 tcatccgctc tcgactgttt ttttgcccct tctgagtcgt ttgattttcg cgtgccatct
     7021 cgattacaat gttttccggt ttgtgacgtc ccatgacctt aactagctca tccactactt
     7081 tgactgtctg gagtatgccc tttttgatgg ctggcgaacc agcaagattc gcaatatgtt
     7141 cgtgcaatga gtccccttgt ccggaaacct gtgccttttg tatatcctct ttgaaggtta
     7201 aagagtcatc atggatcagc tgcataaagt tcctattggc gaagccgtcg ctctttagaa
     7261 aatcgagaat agttttacca ctttgcttgt ctcttatccc gttgataagt ttccgcgaca
     7321 atcgtcccca gcccgtatag cgacgcctct ttaactgttt cataacctta tcgtcgaaca
     7381 ggtgagcgta tgtttttagt ctttcctcaa tcatttcccg atcttcaaag agggtaagag
     7441 tcaacactat atcttctaag atatcttcat tctcttcgtt atccaggaag tccttatctt
     7501 taattatctt taggaggtca tgatacgtac caagtgacgc attaaatcga tcttctaccc
     7561 cggagatctc gacagaatcg aagcattcaa ttttcttaaa gtagtcctct ttcaattgct
     7621 taactgtcac tttgcggttg gtcttgaata acagatctac tattgctttc ttctgttctc
     7681 cgcttagaaa ggcgggttta cgcatgccct cagtgacata cttaactttc gtgagttcat
     7741 tgtacactgt gaaatactcg taaagtaaac tgtgcttagg caatactttt tcgttcggta
     7801 aattcttgtc aaagttggtc atcctctcga tgaacgattg agctgacgca cctttatcga
     7861 caacttcctc aaaattccat ggagtaatcg tttcttcgga ctttcttgtc atccatgcga
     7921 accgagagtt ccctcgggcc aggggtccca catagtaagg tatgcgaaag gttaggattt
     7981 tctcaatctt ttcacgattg tctttgagga acggataaaa atcctcctgc cttctaagta
     8041 tagcatgcaa ttcgcctaag tggatttgat gtggaatgct accgttgtcg aaagtccgct
     8101 gctttcgcag tagatcttcg cgattgagtt ttacaagcaa ctcttccgtc ccatccatct
     8161 tctctaatat gggtttgata aacttgtaga attcctcttg actcgctccg ccgtcaatat
     8221 aacctgcgta cccgtttttc gactgatcaa agaatatttc cttatatttc tcaggcagtt
     8281 gctgacggac tagggccttg agaagtgtca agtcttggtg atgttcatcg taccttttga
     8341 tcattgaagc ggataacggc gccttggtaa tctcagtatt aactctcagt atgtcagata
     8401 ggaggattgc atcgctaagg tttttggcag ccaaaaataa gtccgcatac tgatctccaa
     8461 tttgtgccag tagattgtcg agatcgtcat cgtacgtgtc cttactaagc tgcaatttgg
     8521 catcttcagc taagtcgaag ttcgacttaa aatttggtgt caggcctagt gagagcgcta
     8581 taaggttacc gaacaaccca tttttcttct ctccgggtaa ttgtgcgatc aggttttcta
     8641 gccgtcggga tttagagagg cgggcgctaa gaatagcctt cgcatccacg ccacttgcat
     8701 ttatagggtt ctcttcaaac aactgattat aggtttgtac taactggatg aacagtttgt
     8761 cgacatccga gttgtccgga tttagatcac cctcaatgag aaagtgccca cggaacttta
     8821 tcatatgggc aagagccaag tagattaacc tcaggtccgc tttatcagtt gagtcaacta
     8881 gcttttttct gaggtgataa atcgttgggt acttttcatg atatgccacc tcatctacta
     8941 tgtttccaaa gatggggtgc cgttcatgtt tcttgtcctc ttcgacaagg aaggactctt
     9001 ccaaacggtg aaagaaagaa tcgtcaactt tggccatctc attgctaaaa atttcttgta
     9061 agtaacatat tcggttcttg cgacgtgtat accttctccg agcggttcgt ttcaggcgag
     9121 tcgcctctgc cgtttcgcca ctatcgaata ggagggcacc gataagattc tttttaatcg
     9181 aatgacggtc tgtgttcccc aacaccttaa atttctttga aggtactttg tattcatcgg
     9241 ttatgacagc ccatccaacg gaattagtgc cgatggctaa accaatagaa tactttttat
     9301 cagaaccacc agaagaacca ccagaacttt cgggtgtggc ggactctgag gtcccgggag
     9361 tctcgctgcc gctagaacca ccagaag
//
