LOCUS       sequence-319264-        9496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral overexpression of MYB in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Weng H, Huang H, Wu H, Qin X, Zhao BS, Dong L, Shi H, Skibbe J, Shen
            C, Hu C, Sheng Y, Wang Y, Wunderlich M, Zhang B, Dore LC, Su R, Deng
            X, Ferchen K, Li C, Sun M, Lu Z, Jiang X, Marcucci G, Mulloy JC, 
            Yang J, Qian Z, Wei M, He C, Chen J
  TITLE     METTL14 Inhibits Hematopoietic Stem/Progenitor Differentiation and 
            Promotes Leukemogenesis via mRNA m(6)A Modification.
  JOURNAL   Cell Stem Cell. 2018 Feb 1;22(2):191-205.e9. doi: 
            10.1016/j.stem.2017.11.016. Epub 2017 Dec 28.
  PUBMED    29290617
REFERENCE   2  (bases 1 to 9496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2017.11.016"; journalName: "Cell Stem Cell"; date: 
            "2018-02-1- 1"; volume: "22"; issue: "2"; pages: "191-205.e9"
COMMENT     Sequence Label: pmiRA1-MYB
FEATURES             Location/Qualifiers
     source          1..9496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    20..253
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             438..482
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    749..865
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        955..1158
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1109..1129
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1155..1179
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1237..1263
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        3225..3436
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3449..3717
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      3746..3755
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3776..4438
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     misc_feature    4517..5105
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4570..4590)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4988..4999)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5179..5412
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5484..5605
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5521..5540)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5575..5594
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5645..5780
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5813..5829)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5813..5829)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5826..5848)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5837..5853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5861..5867,5868..5885,5886..5891))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5906..5927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6044..6061)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6215..6803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(6974..7765,7766..7834))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7597..7616
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7835..7939)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8007..8025
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8063..8085)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8185..8204
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8398..8420
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8412..8429
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8413..8429
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8444..8670
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8671..8851
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8898..9023
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
       61 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
      121 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
      181 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
      241 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
      301 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
      361 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
      421 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
      481 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
      541 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
      601 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
      661 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
      721 tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca
      781 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
      841 aacaaattac aaaattcaaa attttatcga tactagtatt atgcccagta catgacctta
      901 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      961 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     1021 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     1081 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     1141 gtttatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     1201 tgttttgacc tccatagaag attctagacc accatgtacc catacgatgt tccagattac
     1261 gctgcccgaa gaccccggca cagcatatat agcagtgacg aggatgatga ggactttgag
     1321 atgtgtgacc atgactatga tgggctgctt cccaagtctg gaaagcgtca cttggggaaa
     1381 acaaggtgga cccgggaaga ggatgaaaaa ctgaagaagc tggtggaaca gaatggaaca
     1441 gatgactgga aagttattgc caattatctc ccgaatcgaa cagatgtgca gtgccagcac
     1501 cgatggcaga aagtactaaa ccctgagctc atcaagggtc cttggaccaa agaagaagat
     1561 cagagagtga tagagcttgt acagaaatac ggtccgaaac gttggtctgt tattgccaag
     1621 cacttaaagg ggagaattgg aaaacaatgt agggagaggt ggcataacca cttgaatcca
     1681 gaagttaaga aaacctcctg gacagaagag gaagacagaa ttatttacca ggcacacaag
     1741 agactgggga acagatgggc agaaatcgca aagctactgc ctggacgaac tgataatgct
     1801 atcaagaacc actggaattc tacaatgcgt cggaaggtcg aacaggaagg ttatctgcag
     1861 gagtcttcaa aagccagcca gccagcagtg gccacaagct tccagaagaa cagtcatttg
     1921 atgggttttg ctcaggctcc gcctacagct caactccctg ccactggcca gcccactgtt
     1981 aacaacgact attcctatta ccacatttct gaagcacaaa atgtctccag tcatgttcca
     2041 taccctgtag cgttacatgt aaatatagtc aatgtccctc agccagctgc cgcagccatt
     2101 cagagacact ataatgatga agaccctgag aaggaaaagc gaataaagga attagaattg
     2161 ctcctaatgt caaccgagaa tgagctaaaa ggacagcagg tgctaccaac acagaaccac
     2221 acatgcagct accccgggtg gcacagcacc accattgccg accacaccag acctcatgga
     2281 gacagtgcac ctgtttcctg tttgggagaa caccactcca ctccatctct gccagcggat
     2341 cctggctccc tacctgaaga aagcgcctcg ccagcaaggt gcatgatcgt ccaccagggc
     2401 accattctgg ataatgttaa gaacctctta gaatttgcag aaacactcca atttatagat
     2461 tctttcttaa acacttccag taaccatgaa aactcagact tggaaatgcc ttctttaact
     2521 tccacccccc tcattggtca caaattgact gttacaacac catttcatag agaccagact
     2581 gtgaaaactc aaaaggaaaa tactgttttt agaaccccag ctatcaaaag gtcaatctta
     2641 gaaagctctc caagaactcc tacaccattc aaacatgcac ttgcagctca agaaattaaa
     2701 tacggtcccc tgaagatgct acctcagaca ccctctcatc tagtagaaga tctgcaggat
     2761 gtgatcaaac aggaatctga tgaatctgga attgttgctg agtttcaaga aaatggacca
     2821 cccttactga agaaaatcaa acaagaggtg gaatctccaa ctgataaatc aggaaacttc
     2881 ttctgctcac accactggga aggggacagt ctgaataccc aactgttcac gcagacctcg
     2941 cctgtggcag atgcaccgaa tattcttaca agctccgttt taatggcacc agcatcagaa
     3001 gatgaagaca atgttctcaa agcatttaca gtacctaaaa acaggtccct ggcgagcccc
     3061 ttgcagcctt gtagcagtac ctgggaacct gcatcctgtg gaaagatgga ggagcagatg
     3121 acatcttcca gtcaagctcg taaatacgtg aatgcattct cagcccggac gctggtcatg
     3181 tgagcggccg cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc
     3241 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag
     3301 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     3361 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     3421 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg
     3481 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg
     3541 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct
     3601 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac
     3661 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa
     3721 gctgtgaccg gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc
     3781 catggagatc gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg
     3841 cggcggagag ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg
     3901 cgccctgacc ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt
     3961 cggcacctac cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta
     4021 caccaacacc cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta
     4081 ccgctacgag gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga
     4141 ggacagcgtg atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca
     4201 ccccatgggc gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg
     4261 cggctactac agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag
     4321 catcctgcag aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa
     4381 caccgagctg ggcatcgtgg agtaccagca cgccttcaag acccccattg ccttcgccag
     4441 atcccgcgct cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg
     4501 atctcgctaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     4561 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     4621 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     4681 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     4741 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     4801 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4861 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     4921 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4981 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     5041 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     5101 cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     5161 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagataa gatctgcttt
     5221 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     5281 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     5341 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     5401 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     5461 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     5521 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5581 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     5641 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     5701 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     5761 aggaggcttt tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc
     5821 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     5881 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     5941 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6001 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6061 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6121 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6181 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6241 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6301 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6361 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6421 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6481 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6541 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6601 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6661 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6721 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6781 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6841 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6901 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     6961 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7021 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7081 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7141 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7201 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7261 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7321 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7381 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7441 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7501 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7561 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7621 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7681 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7741 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7801 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7861 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     7921 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     7981 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     8041 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     8101 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     8161 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     8221 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     8281 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     8341 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     8401 agtcacgacg ttgtaaaacg acggccagtg ccaagctgac gcgtgtagtc ttatgcaata
     8461 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     8521 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     8581 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     8641 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
     8701 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8761 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8821 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     8881 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     8941 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     9001 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     9061 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     9121 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     9181 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     9241 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     9301 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     9361 cggccactga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     9421 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     9481 agagtggtgc agagag
//
