LOCUS       sequence-319265-        3120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses opto-RhoA (RhoA-GGGSx2-BcLOV4-GGGSx2-mCherry) in a 
            pcDNA3.1 backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Berlew EE, Kuznetsov IA, Yamada K, Bugaj LJ, Boerckel JD, Chow BY
  TITLE     Single-Component Optogenetic Tools for Inducible RhoA GTPase 
            Signaling.
  JOURNAL   Adv Biol. 2021 Sep;5(9):e2100810. doi: 10.1002/adbi.202100810.
  PUBMED    34288599
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Adv Biol.";
            date: "2021-09"; volume: "5(9):e2100810. doi"; pages: " 
            10.1002/adbi.202100810"
COMMENT     Sequence Label: opto-RhoA-mCherry_pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..3120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2407..3117
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2552..2570)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2820..2839
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgtcagctg ccatccggaa gaaactggtg attgttggtg atggagcctg tggaaagaca
       61 tgcttgctca tagtcttcag caaggaccag ttcccagagg tgtatgtgcc cacagtgttt
      121 gagaactatg tggcagatat cgaggtggat ggaaagcagg tagagttggc tttgtgggac
      181 acagctgggc aggaagatta tgaccgcctg aggcccctct cctacccaga taccgatgtt
      241 atactgatgt gtttttccat cgacagccct gatagtttag aaaacatccc agaaaagtgg
      301 accccagaag tcaagcattt ctgtcccaac gtgcccatca tcctggttgg gaataagaag
      361 gatcttcgga atgatgagca cacaaggcgg gagctagcca agatgaagca ggagccggtg
      421 aaacctgaag aaggcagaga tatggcaaac aggattggcg cttttgggta catggagtgt
      481 tcagcaaaga ccaaagatgg agtgagagag gtttttgaaa tggctacgag agctgctctg
      541 caagctagac gtgggaagaa aaaatctggg ggtggcggat caggaggagg gtcaatggcc
      601 acagacgcaa tcgaccatta ctaccagaac agagacggcc ccccttccag gagctttagc
      661 gacgaatacg actcatacaa taagacagcc cctctgccac ctctgagcgc ctgcccagag
      721 cgcgcccaga cccctaacag caagagaatc cccatcacac ccaattccgc caagagcacc
      781 tccgagtctc ctcggagaat cacattctct cgcgacgcag gcagctccac caagggcagc
      841 acccagacaa gcccatccaa ccagggctcc gtgtctagcc ggaatggctc taacggaaat
      901 ggcctgggcc tgtcctctgc cggcaacttc gccgatttct ttagctccga ggtgttttat
      961 atcgtgctgc acaatcccac cacagcccac agattcctga agttttgcca gagcagggcc
     1021 tgttccgaga acctggagtt tctgcagaag atcgacgagt acaataggct gatcgatgag
     1081 gccacacgcc tgctgtctac catctacagc acatacgtga gcagcgaggc ccctaagcag
     1141 atcaacatct ctaatagcca cgccaggcgc ctgagccacg acatcaaggg agcaacccac
     1201 aacgtgctgc ccggcctgga ggatatcttc aatggctccc aggagcagat cgagaagctg
     1261 ctggcctctg acctgtatcc tagatttgtg aagcaccagg tgaccgcatc cgccacaatg
     1321 gccctggcaa acgacaagga gaggtaccca ggcctgggcg attgcttctg tctgacagac
     1381 ccacggagag ccgataatcc catcgtgttc gcaagcgacg gattcgtgag cgtgaccggc
     1441 tattcccgct ctgatatcat cccaaggaac tgccgcttcc tgcagggcag ctttaccgac
     1501 cggcaggcca caaagaggct gcgcacctcc atcgagaatc gcgaggagac agtggagctg
     1561 ctgctgaact atcggaagaa tggcgatccc ttttggaacc tgctgtacgt gtcccctctg
     1621 ctggacggca atggcgatgt gagattcttt ctgggcggcc agatcaactg ttctaccaca
     1681 atccacagcc ggaccgacgt gctgagaatc ctgagcctga acgacgagga tacaggctcc
     1741 ttcatcgagg gctctaataa gacccccagc gtgcggtcca aggagtctaa caatgcctcc
     1801 aactatggca agacatcctt ctttaagtct tttaagaagt acaaggccaa taccgtggag
     1861 atcagagacg aggccggcat ggagggcgag ctgatcgata ggatcggcaa gctgcagttc
     1921 aagacacagg tggaggagtt ttacaccgcc tattctaagt acctggtgct gagctatcag
     1981 ccacagtccc agaacctgat cgtgaagtac ttcagccccg gcatcgtgga tatgctgaac
     2041 ctgaatatgc ctaatggcct ggtggcccca atcgtggaca aggatatctt caaggtgctg
     2101 acagagcact ccccttcctc tgtgccacgg acctttaaga acgtggtgag ggaaggcatc
     2161 aaagcaggca gggccgtgtc tgtggagaca ggcctgctga caggcatcga ggagatcaag
     2221 aagtctcaga acatcttcag cggcaccaca gccaatcggg agagaggctg gaagaaggcc
     2281 gaggagaagt acgtgtgcca ctttaccccc tgtaaggatg agatgggcag ggtcggctgg
     2341 gtggtgctga ctgtcgctcc taaactggaa agatttgaat tcggtggtgg atcaggtgga
     2401 ggatctatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     2461 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     2521 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     2581 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     2641 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     2701 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     2761 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     2821 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     2881 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     2941 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     3001 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     3061 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaatga
//
