LOCUS       sequence-319267-        8680 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector to TH gene locus for induction of P2A-fused 
            tdTomato, with floxed PGK-puroR drug resistant cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Xiong M, Tao Y, Gao Q, Feng B, Yan W, Zhou Y, Kotsonis TA, Yuan T, 
            You Z, Wu Z, Xi J, Haberman A, Graham J, Block J, Zhou W, Chen Y, 
            Zhang SC
  TITLE     Human Stem Cell-Derived Neurons Repair Circuits and Restore Neural 
            Function.
  JOURNAL   Cell Stem Cell. 2020 Sep 16. pii: S1934-5909(20)30410-0. doi: 
            10.1016/j.stem.2020.08.014.
  PUBMED    32966778
REFERENCE   2  (bases 1 to 8680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2020 Sep 16. pii: S1934-5909(20)30410-0. doi: 
            10.1016/j.stem.2020.08.014."
COMMENT     Sequence Label: Th-tdTomato
FEATURES             Location/Qualifiers
     source          1..8680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             996..1052
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1053..2483
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    2517..2550
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2572..3048
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2771..2790)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     promoter        3109..3608
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3144..3167)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3541..3560
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3648..4247
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3648..3667)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     protein_bind    4804..4837
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5869..5888)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5870..5888)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5895..5912)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5895..5911)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5904..5926)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(6052..6507)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6189..6210)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6401..6420
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6534..6638
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6639..6707,6708..7499)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6857..6876)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7670..8258
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8159..8178
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8412..8429
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8546..8567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8582..8587,8588..8605,8606..8612)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8620..8636
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8625..8647
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8644..8660
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8644..8660
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattaaccct cactaaaggg aacaaaagct ggtaccggcc agccaggcct tccttctggc
       61 cttgagcagg gctggacctg tgagcccagg tcacagatga gaaaaccgac ccctggctgc
      121 agcagccccc acacagcagg gacaccatcc gtgagaagga ccccagcgtc tggggagggg
      181 cagacctaca ggactggggg ctgctgggtg gccgggtcaa ggccagtctt ggaggtgctg
      241 acagagcctg agctttgtga ggacgtcctg tggaacctgt cccggccccc tgccctggga
      301 tggggagaag tcagggggat agacagagtc aaggtggggg acagggcggg agtggggtcc
      361 ccagggctgg gggcctttgg tgcagtgacc agagtgtcag gagaggggag caaagccctc
      421 tagcctcatc ctcataaaag gtctcatcat tttccctcca gcctcttatg cactggggaa
      481 actgaggcca ggggctatgt gtccagcgga caggggtgct gaattccacc cacaggctta
      541 gggatatggt caaggaaagc ttcctggagg aggcccagtg gaggttcagg gagggatggg
      601 gtgcccggca gtctctagtg gaaaaggcgc ctagcctatc tcccccatga accccctcac
      661 ccagccctgg aagaggcctc agtgtcccgc ctgtgaccag ttggctcaga aaagccctgg
      721 gagctctgag ccactgtgaa ggtggaaacg cggcccctgg cctcccctct cctggaggct
      781 gcagactctg cccgccagtt gacgagggct ctgccgctct cctccccagg agctatgcct
      841 cacgcatcca gcgccccttc tccgtgaagt tcgacccgta cacgctggcc atcgacgtgc
      901 tggacagccc ccaggccgtg cggcgctccc tggagggtgt ccaggatgag ctggacaccc
      961 ttgcccatgc gctgagtgcc attggcggat ccggcgcaac aaacttctct ctgctgaaac
     1021 aagccggaga tgtcgaagag aatcctggac cgatggtgag caagggcgag gaggtcatca
     1081 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
     1141 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     1201 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
     1261 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
     1321 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
     1381 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
     1441 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     1501 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
     1561 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
     1621 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
     1681 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg gggcatggca
     1741 ccggcagcac cggcagcggc agctccggca ccgcctcctc cgaggacaac aacatggccg
     1801 tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt
     1861 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     1921 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt
     1981 acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct
     2041 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg
     2101 tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca
     2161 ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca
     2221 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc
     2281 tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg
     2341 tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg
     2401 actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctgtacg
     2461 gcatggacga gctgtacaag taatgagaat tccgatcata ttcaataacc cttaatataa
     2521 cttcgtataa tgtatgctat acgaagttat agcgctgctc gagagatcta cgggtggcat
     2581 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
     2641 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     2701 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     2761 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     2821 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc
     2881 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     2941 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     3001 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttta ggtctgaaga
     3061 ggagtttacg tccagccacg cgtaggatct cgacctcgaa attctaccgg gtaggggagg
     3121 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     3181 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccgactc
     3241 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     3301 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     3361 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     3421 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     3481 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     3541 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     3601 gcctttcgac ctgcatccat ctagatctcg agcagctgaa gcttaccatg accgagtaca
     3661 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     3721 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     3781 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     3841 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     3901 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     3961 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     4021 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     4081 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     4141 cccacaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     4201 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
     4261 acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcgatgata tcagatcccc
     4321 gggatgcaga aattgatgat ctattaaaca ataaagatgt ccactaaaat ggaagttttt
     4381 cctgtcatac tttgttaaga agggtgagaa cagagtacct acattttgaa tggaaggatt
     4441 ggagctacgg gggtgggggt ggggtgggat tagataaatg cctgctcttt actgaaggct
     4501 ctttactatt gctttatgat aatgtttcat agttggatat cataatttaa acaagcaaaa
     4561 ccaaattaag ggccagctca ttcctcccac tcatgatcta tagatctata gatctctcgt
     4621 gggatcattg tttttctctt gattcccact ttgtggttct aagtactgtg gtttccaaat
     4681 gtgtcagttt catagcctga agaacgagat cagcagcctc tgttccacat acacttcatt
     4741 ctcagtattg ttttgccaag ttctaattcc atcagaagct ggtcgagatc cggaaccctt
     4801 aatataactt cgtataatgt atgctatacg aagttattag gtccctcgaa gaggttcact
     4861 aggatcttag gtgcacggcg tccctgaact cccttcccaa cctcgtttgg tcctgcactg
     4921 tcccggaatc caggccctgg tgaggggctg ggtcccgggt gccccccatg ccctccctgc
     4981 tgccaggctc ccactgcccc tgcacctgct tctcagcgca acagctgtgt gtgcccgtgg
     5041 tgaggttgtg ctgcctgtgg tgaggtcctg tcctggctcc cagggtcctg ggggctgctg
     5101 cactgccctc cgcccttccc tgacactgtc tgctgcccca atcaccgtca caataaaaga
     5161 aactgtggtc tctacacctg cctggcccca catctgtgcc acagagacag accctgggat
     5221 cctcagactc ccacaccccc accccagcct cactcagagg tttcgccctg gcctccttcc
     5281 tcctctggga gatggctggc cgccctggcc aggcagctgg cccctccggg cctggtttcc
     5341 ccgctcaccc tgaggccccg cccagctctg agccccaagc agctccagag gctcgggcac
     5401 cctggccgag ctgccccatc tccgtggggt gccctcccaa ggtggggagc cacgtgacag
     5461 tgggagggcc tctctcaggc ctggaaggga gcaggggtca caaactgtgc tggctggggg
     5521 tggtctcaga ggtgggcctg caggcctaac cctccctgct gacagggctc ccagcccttg
     5581 agagaaacag ggatggagga acagctgccc tgatgccctc acccacccgg agcaggccct
     5641 gcgaaccaag gggaacctca gtgtggcccc cagcatgtgt gctgatgggg agggtctggc
     5701 tgagctggtg cccaggcaga tggtctgggc ctgtctcccc agcgaggcag gatgggggct
     5761 ggatttcaga ctctgtaaga tgcccctggc ttactcgagg ggcctggaca ttgccctcca
     5821 gagagagcac ccaacacgcg gccgccaccg cggtggagct ccaattcgcc ctatagtgag
     5881 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     5941 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     6001 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gaaattgtaa
     6061 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     6121 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     6181 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     6241 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     6301 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     6361 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     6421 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     6481 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     6541 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6601 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6661 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6721 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6781 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6841 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6901 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6961 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7021 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7081 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7141 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7201 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7261 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7321 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7381 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7441 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7501 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7561 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7621 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7681 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7741 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7801 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7861 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7921 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7981 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8041 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8101 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8161 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8221 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8281 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8341 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8401 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     8461 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     8521 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     8581 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     8641 acacaggaaa cagctatgac catgattacg ccaagctcga
//
