LOCUS       sequence-319277-        4384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mobius Assembly cloning and expression vector for plants with 
            high-copy number origin of replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: pMAP L2-Delta
FEATURES             Location/Qualifiers
     source          1..4384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(66..857)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1105..1693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1594..1613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3215..3239
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    3266..3290
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        3342..3376
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             3385..3396
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      4127..4198
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4214..4241
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 aaaaaacacc cgttagggtg tttttagtta gtgtggcgcg ccgacttgcg acatgcggtc
       61 ctttgttatt tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc
      121 aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct
      181 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca
      241 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact
      301 acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt
      361 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct
      421 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc
      481 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt
      541 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct
      601 acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc
      661 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca
      721 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc
      781 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg
      841 atcaccgctt ccctcatgat gtttaacttt gttttagggc gactgccctg ctgcgtaaca
      901 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat
      961 gcccgaggca tagactgtac cccaaaaaaa cagtcataac aagccatgaa aaccgccact
     1021 gcgccgttac caccgctgcg ttcggtcaag gttcgatcgg attggcggtt atgcggttcc
     1081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1741 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1801 ctttgagtga gctgccgatc cgccgccagt tcaggccgtc cggccccgga ttctgaccat
     1861 aatttcatcg aaaaaagggg cgcagccctt cttgttctaa tagttctata agttcaggcg
     1921 aaaatcgtgc agcaattaca aaaggttgcg cgtctataag tggggaatcc agccgcaaaa
     1981 gtggggaatc cagccgcaaa agtggggaat ccagccgaaa tcgcggattg acgagtggga
     2041 tttcccgccc aataaatcca ccatgggaaa gacactcgac gttgcccgcg accgggcctt
     2101 tgaccagacc gcgaccgtgc tgcccgccga aatggcgcgg ggggtctata tgcgcaacgc
     2161 ccccagcctc gcggccctga agctgatgca tctgatgatc gccacggcgg gcgggcgcat
     2221 ggccgatgac gtgcgccacg aaatgcggct ggccgacatc cgcaagatcg acggtatgga
     2281 taaccacacc cgggccagcc tgaccccgct ctttgcggaa ctggcggcgg cggtgctgac
     2341 ccacgacgac ccggaaaagc gggtcgtgac catcggtggc ctgctggacg aagcccggat
     2401 cgattaccgg cacgaggtca gcggcgatct tctggtgtcg tggaccttcc gcagcatgtt
     2461 ccgccgcatg gcggcggaat cgaaccattg ggcgattctc gaccggcaga ccgtgttcca
     2521 cctcggcagc aagtattccg tgctgttgtt ccagcacatc gccagcttca aggaatacga
     2581 ccacattacc ggcaagacct ttaccgtgcc ggagttgcgg gctgtgtttg gtatccccga
     2641 gggcaaaatc aagcgtttcg cagacctcaa cagagacgtg ctgacgcccg ccattgccga
     2701 aatcaaccag ctttcccgcc tgactctgac cgccacgccg aacaagatcg ggcgcaccgt
     2761 ggccagcgtg acgattgctt gggaagaaaa gcccctcgaa ggcaagcgct cgaccaaggc
     2821 cgaactggac cgcccgaagg tgggccggaa ggcccggcgc gacggcaccg ccgagacggt
     2881 ggcacgggcc ttcccggcat cgggcgggat cgagttcgac cagcattggc gcgacctcaa
     2941 gcgggcggcg ggctgcaaca tggacaacac catgatcgcc gacaaattcc gggcatggtg
     3001 cgccgggaag ggcctcgctc tcgatgcccg gaacatcgaa caggcgttca gcagcttctg
     3061 caccaaggtg ggccgggtct gaccatcatc agttcggtgg tcttccgacg aacaataagg
     3121 ccgcaaatcg cggccttttt tattgataac aaaaccggct cagttctgcg tagaaaccaa
     3181 catgcaagct ccaccgggtg caaagcggca gcggcggcag gatatattca attgtaaatg
     3241 gcttcatgtc cgggaaatct actggtggca ggatatattg tggtgtaaac aatggagaaa
     3301 aagattctgg aattcggtct cacttgggag atatgcaggt gtttacggct agctcagtcc
     3361 taggtatagt gctagctact agagaaagag gagaaatact aaatgcgtaa aggcgaagaa
     3421 ctgttcacgg gcgtagttcc gattctggtc gagctggacg gcgatgtgaa cggtcataag
     3481 tttagcgttc gcggtgaagg tgagggcgac gcgaccaacg gcaaactgac cctgaagttc
     3541 atctgcacca ccggtaaact gccggtgcct tggccgacct tggtgacgac gttgacgtat
     3601 ggcgtgcagt gttttgcgcg ttatccggac cacatgaaac aacacgattt cttcaaatct
     3661 gcgatgccgg agggttacgt ccaggagcgt accatttcct tcaaggatga tggctactac
     3721 aaaactcgcg cagaggttaa gtttgaaggt gacacgctgg tcaatcgtat cgaattgaag
     3781 ggtatcgact ttaaagagga tggtaacatt ctgggccata aactggagta taacttcaac
     3841 agccataatg tttacattac ggcagacaag caaaagaacg gcatcaaggc caatttcaag
     3901 attcgccaca atgttgagga cggtagcgtc caactggccg accattacca gcagaacacc
     3961 ccaattggtg acggtccggt tttgctgccg gataatcact atctgagcac ccaaagcgtg
     4021 ctgagcaaag atccgaacga aaaacgtgat cacatggtcc tgctggaatt tgtgaccgct
     4081 gcgggcatca cccacggtat ggacgagctg tataagtaat gagcttcaaa taaaacgaaa
     4141 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     4201 ctagagtcac actggctcac cttcgggtgg gcctttctgc gcacctgcat atacccctgc
     4261 aggaattgtt gattttgtga tgactgatgg caggatatat gcggttgtaa ttcattttta
     4321 ttgtctaaat ttctgtattt gtttgtttgt tcggttgtaa atttttttgg aagaacaaga
     4381 aaag
//
