LOCUS       sequence-319310-        8759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains integrin alpha v residues 1–960 with the M400C mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Wang J, Dong X, Zhao B, Li J, Lu C, Springer TA
  TITLE     Atypical interactions of integrin alphaVbeta8 with pro-TGF-beta1.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 May 23;114(21):E4168-E4174. doi: 
            10.1073/pnas.1705129114. Epub 2017 May 8.
  PUBMED    28484027
REFERENCE   2  (bases 1 to 8759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1705129114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-05-23- 23"; volume: "114"; issue: "21"; pages: 
            "E4168-E4174"
COMMENT     Sequence Label: Integrin-alpha-v Ectodomain M400C
FEATURES             Location/Qualifiers
     source          1..8759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        133..512
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        513..716
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     667..687
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     761..780
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        761..779
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3801..3824
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3888..3899
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4032..4049
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4079..4096)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4085..4309
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4355..4783
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4442..4461)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4652..4673
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4792..4812)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4797..5126
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4977..5112
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5039..5058
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5175..6200
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5175..6200
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6330..6451
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6367..6386)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6421..6440
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6500..6516)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6500..6516)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6513..6535)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6524..6540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6548..6554,6555..6572,6573..6578))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6593..6614
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6731..6748)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6902..7490)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6982..7001)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7661..8452,8453..8521))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8284..8303
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8522..8626)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8701..8720)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
       61 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
      121 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      181 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      241 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      301 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      361 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      421 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      481 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      541 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      601 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      661 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct
      721 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
      781 agacccaagc tggctagcgt ttaaacgggc cctctagaac catggctttt ccgccgcggc
      841 gacggctgcg cctcggtccc cgcggcctcc cgcttcttct ctcgggactc ctgctacctc
      901 tgtgccgcgc cttcaaccta gacgtggaca gtcctgccga gtactctggc cccgagggaa
      961 gttacttcgg cttcgccgtg gatttcttcg tgcccagcgc gtcttcccgg atgtttcttc
     1021 tcgtgggagc tcccaaagca aacaccaccc agcctgggat tgtggaagga gggcaggtcc
     1081 tcaaatgtga ctggtcttct acccgccggt gccagccaat tgaatttgat gcaacaggca
     1141 atagagatta tgccaaggat gatccattgg aatttaagtc ccatcagtgg tttggagcat
     1201 ctgtgaggtc gaaacaggat aaaattttgg cctgtgcccc attgtaccat tggagaactg
     1261 agatgaaaca ggagcgagag cctgttggaa catgctttct tcaagatgga acaaagactg
     1321 ttgagtatgc tccatgtaga tcacaagata ttgatgctga tggacaggga ttttgtcaag
     1381 gaggattcag cattgatttt actaaagctg acagagtact tcttggtggt cctggtagct
     1441 tttattggca aggtcagctt atttcggatc aagtggcaga aatcgtatct aaatacgacc
     1501 ccaatgttta cagcatcaag tataataacc aattagcaac tcggactgca caagctattt
     1561 ttgatgacag ctatttgggt tattctgtgg ctgtcggaga tttcaatggt gatggcatag
     1621 atgactttgt ttcaggagtt ccaagagcag caaggacttt gggaatggtt tatatttatg
     1681 atgggaagaa catgtcctcc ttatacaatt ttactggcga gcagatggct gcatatttcg
     1741 gattttctgt agctgccact gacattaatg gagatgatta tgcagatgtg tttattggag
     1801 cacctctctt catggatcgt ggctctgatg gcaaactcca agaggtgggg caggtctcag
     1861 tgtctctaca gagagcttca ggagacttcc agacgacaaa gctgaatgga tttgaggtct
     1921 ttgcacggtt tggcagtgcc atagctcctt tgggagatct ggaccaggat ggtttcaatg
     1981 atattgcaat tgctgctcca tatgggggtg aagataaaaa aggaattgtt tatatcttca
     2041 atggaagatc aacaggcttg aacgcagtcc catctcaaat ccttgaaggg cagtgggctg
     2101 ctcgaagctg cccaccaagc tttggctatt caatgaaagg agccacagat atagacaaaa
     2161 atggatatcc agacttaatt gtaggagctt ttggtgtaga tcgagctatc ttatacaggg
     2221 ccagaccagt tatcactgta aatgctggtc ttgaagtgta ccctagcatt ttaaatcaag
     2281 acaataaaac ctgctcactg cctggaacag ctctcaaagt ttcctgtttt aatgttaggt
     2341 tctgcttaaa ggcagatggc aaaggagtac ttcccaggaa acttaatttc caggtggaac
     2401 ttcttttgga taaactcaag caaaagggag caattcgacg agcactgttt ctctacagca
     2461 ggtccccaag tcactccaag aacatgacta tttcaagggg gggactgatg cagtgtgagg
     2521 aattgatagc gtatctgcgg gatgaatctg aatttagaga caaactcact ccaattacta
     2581 tttttatgga atatcggttg gattatagaa cagctgctga tacaacaggc ttgcaaccca
     2641 ttcttaacca gttcacgcct gctaacatta gtcgacaggc tcacattcta cttgactgtg
     2701 gtgaagacaa tgtctgtaaa cccaagctgg aagtttctgt agatagtgat caaaagaaga
     2761 tctatattgg ggatgacaac cctctgacat tgattgttaa ggctcagaat caaggagaag
     2821 gtgcctacga agctgagctc atcgtttcca ttccactgca ggctgatttc atcggggttg
     2881 tccgaaacaa tgaagcctta gcaagacttt cctgtgcatt taagacagaa aaccaaactc
     2941 gccaggtggt atgtgacctt ggaaacccaa tgaaggctgg aactcaactc ttagctggtc
     3001 ttcgtttcag tgtgcaccag cagtcagaga tggatacttc tgtgaaattt gacttacaaa
     3061 tccaaagctc aaatctattt gacaaagtaa gcccagttgt atctcacaaa gttgatcttg
     3121 ctgttttagc tgcagttgag ataagaggag tctcgagtcc tgatcatatc tttcttccga
     3181 ttccaaactg ggagcacaag gagaaccctg agactgaaga agatgttggg ccagttgttc
     3241 agcacatcta tgagctgaga aacaatggtc caagttcatt cagcaaggca atgctccatc
     3301 ttcagtggcc ttacaaatat aataataaca ctctgttgta tatccttcat tatgatattg
     3361 atggaccaat gaactgcact tcagatatgg agatcaaccc tttgagaatt aagatctcat
     3421 ctttgcaaac aactgaaaag aatgacacgg ttgccgggca aggtgagcgg gaccatctca
     3481 tcactaagcg ggatcttgcc ctcagtgaag gagatattca cactttgggt tgtggagttg
     3541 ctcagtgctt gaagattgtc tgccaagttg ggagattaga cagaggaaag agtgcaatct
     3601 tgtacgtaaa gtcattactg tggactgaga cttttatgaa taaagaaaat cagaatcatt
     3661 cctattctct gaagtcgtct gcttcattta atgtcataga gtttccttat aagaatcttc
     3721 caattgagga tatcaccaac tccacattgg ttaccactaa tgtcacctgg ggcattcagc
     3781 cagcgcccat gaccggtgga ctggaggtgc tcttccaagg ccccggagaa aatgctcagc
     3841 tggaaaaaga gctccaggca cttgagaagg aaaatgcaca gctggaatgg gaattacaag
     3901 cactagagaa ggagctagct caaaccactg gttggagagg tggccatgtt gtggaaggct
     3961 tggctggtga attggaacaa ttgagagccc gtctggagca tcacccacaa ggccaaagag
     4021 aaccagcggg ccatcaccat caccatcact aaaagcttaa gtttaaaccg ctgatcagcc
     4081 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     4141 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     4201 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     4261 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     4321 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     4381 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4441 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4501 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4561 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4621 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4681 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4741 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     4801 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4861 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     4921 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     4981 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5041 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     5101 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     5161 ctgatcagca cgtgatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
     5221 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
     5281 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
     5341 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     5401 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     5461 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     5521 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     5581 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
     5641 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
     5701 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
     5761 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
     5821 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
     5881 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
     5941 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
     6001 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
     6061 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
     6121 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
     6181 gtccgagggc aaaggaatag cacgtgctac gagatttcga ttccaccgcc gccttctatg
     6241 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     6301 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     6361 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6421 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     6481 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6541 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6601 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6661 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6721 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6781 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6841 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6901 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6961 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7021 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7081 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7141 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7201 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7261 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7321 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7381 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7441 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7501 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7561 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7621 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7681 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7741 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7801 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7861 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7921 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7981 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8041 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8101 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8161 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8221 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8281 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8341 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8401 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8461 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8521 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8581 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8641 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     8701 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgg
//
