LOCUS       sequence-319311-        3636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Three-fragment Gateway compatible promoter with PATCs for germline 
            expression from arrays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Aljohani MD, El Mouridi S, Priyadarshini M, Vargas-Velazquez AM, 
            Frokjaer-Jensen C
  TITLE     Engineering rules that minimize germline silencing of transgenes in 
            simple extrachromosomal arrays in C. elegans.
  JOURNAL   Nat Commun. 2020 Dec 9;11(1):6300. doi: 10.1038/s41467-020-19898-0.
  PUBMED    33298957
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-12-9"; volume: "11(1):6300. doi"; pages: " 
            10.1038/s41467-020-19898-0"
COMMENT     Sequence Label: pCFJ1117 - [4-1] - Promoter - Psmu-1(no_atg)
FEATURES             Location/Qualifiers
     source          1..3636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1681..1805
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1877..1896)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1878..1896)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1901..1917)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1901..1917)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2019..2038)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2030..2839
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2097..2116)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2986..3574
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3475..3494
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgaa atttttcagg cgggaagtgg cttcggtgcg
      721 ttcaacaaac gcgcttttga ctcgctagcc ggatccggct tcagcggttt tgacaaaaga
      781 gctttcgatt cactggctgg acaaggtttt actggatttg agaaacgcgc ctttgataca
      841 gtggtacggt tttcggtgga aattccagtt tttcgaattt tcaataattt tcagagcacg
      901 tcaggctttg acgatttcaa attgtgaaga gctcactgat atttaaaatt tggttcaatt
      961 caagcacata tttttgaggt aagcaactga ttttaatgtt gttaatcaaa tgaaaagtga
     1021 aatttttgga ataatttttt tttgttttca ggtagctcca acgaacgaca cacacaagcc
     1081 cagtccccca gtattcctga cgacagcccc tgctcacaca ctttttatta taattccttt
     1141 tttttcaaac ttttttttca aattttatac tttaatctca aaaaacctat tcatacgaac
     1201 acaagtaccg gtgtgcgtgt gtgagaataa gaatttattt taaaaaaata aagaatgtcg
     1261 tcctgttttt tcatatttat tgaagaaaat ggagctggaa aaaaatcaat tgtaaaaaac
     1321 agaaaaacgt gctgataaaa tgcattgtac gcaaactgtg gattttgaga actaacgtag
     1381 tcaagcaaaa atctctggaa ttttatcgat ttttaacgga taaactgttg ttttttgtaa
     1441 ctgtgcatga aaaaaaatct caggcaaaaa tattttctga aaaaaaaaga gtttaaacac
     1501 aaatgcaagt ccacaaaaaa ctccaatttg aaattcgctc caaaaacgca aaacacgcca
     1561 agcgctcgtg ctggcgcatt tgccgatcgc ttccaaaaag gctggcgcac ttctcgtgtc
     1621 cgaggatatt cgaattttac acgtcatttt cagccgtttt tcaccgaatt ttcgctgaaa
     1681 acaagtttgt acaaaaaagt tgaacgagaa acgtaaaatg atataaatat caatatatta
     1741 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatgcagtca
     1801 ctatgaacca actacttaga tggtattagt gacctgtaga attcgagctc tagagctgca
     1861 gggcggccgc gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     1921 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     1981 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     2041 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     2101 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     2161 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     2221 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     2281 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     2341 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     2401 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     2461 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     2521 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     2581 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     2641 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     2701 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     2761 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     2821 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
     2881 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc
     2941 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3001 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3061 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3121 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3181 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3241 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3301 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3361 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3421 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3481 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3541 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3601 gttcctggcc ttttgctggc cttttgctca catgtt
//
