LOCUS       sequence-319313-        4707 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Three-fragment Gateway compatible promoter with PATCs for germline 
            expression from arrays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Aljohani MD, El Mouridi S, Priyadarshini M, Vargas-Velazquez AM, 
            Frokjaer-Jensen C
  TITLE     Engineering rules that minimize germline silencing of transgenes in 
            simple extrachromosomal arrays in C. elegans.
  JOURNAL   Nat Commun. 2020 Dec 9;11(1):6300. doi: 10.1038/s41467-020-19898-0.
  PUBMED    33298957
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-12-9"; volume: "11(1):6300. doi"; pages: " 
            10.1038/s41467-020-19898-0"
COMMENT     Sequence Label: pMDJ80 - pENTR[4-1] - Promoter - Psmu-2 (no_st...
FEATURES             Location/Qualifiers
     source          1..4707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..18
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2..18
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    58..153
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    2218..2341
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2413..2432)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2414..2432)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2437..2453)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2437..2453)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2555..2574)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2566..3375
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2633..2652)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3522..4110
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4011..4030
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4264..4281
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4440..4467)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4559..4645)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(4694..4707,1..9)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgtaaaacga cggccagtct taagctcggg cccgagttaa cgctaccatg gagctccaaa
       61 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
      121 tttttataat gccaactttg tatagaaaag ttgtgcacca atttgagttt ccatggggaa
      181 tcaagcggat accgattgag ttgaagaacc ctaaataaaa ttttatatat tttaccttcc
      241 aacttcgaaa aaatcactca ccattttagc atgtttccct caaaaaccgt ggcaaattgc
      301 tcttggaaaa taatagacac atcagttggt cgagcatttt ttggaggaat tttaaaactg
      361 tgatgatggt tgaaggtgac tatattgtct gcgagttgat actctggaat attgattttt
      421 ttgaagattt catagtcttc ttggatttca aaaattgaaa tatttcagct gtattaaaag
      481 agaataaata aaatgatatt gcctaggggt gagcggcaaa cgattttttc cggcaaatcg
      541 gcaaatcgac aaattgccgg aattggaaat ttccagcaaa tcggcatatc ggcaatttgc
      601 cggaatttaa aatttccggc aaaccggcaa accggcaaat tgccgatttg ccgaacttcg
      661 gtaaatttgc aaatcggtaa tttgccgaaa attttcggca aattgtggtt ttgcacttat
      721 tttttggaat ttcaatttca aatggcaaaa ttaaacgcat catatgaatt tttctatatc
      781 tattttgaga agttctatga aaatatgtag aaaaaacggg aaaaattcaa taatagacag
      841 ttttaagtgc ttccgtctta taaaaaatcc ctctaaacat ttccggcaaa tctggtattc
      901 ggcaaatcgg caagtcggca atttgccgta aattaaaatt tccggcaaaa cggcaaaccg
      961 gcaaattgcc ggaattggaa atttccggca aatcggcaaa ttgcttgccc attttttgat
     1021 tcttagtcgg cgttttacac aaaagataaa ttccaataaa agacagaact ttgctttttg
     1081 ccagaaatta aaaaattggg gcaaaccaat ccaaatatga agtgtaaact tgggtttgca
     1141 ccagggctgt gcggaaaccg gcaattgccg aaattgccga accctaaaaa ctttggcaac
     1201 cggcaacttt tgctggctgg aacttcggca acttttcggc aaacggcaac ttggttgccg
     1261 gttgcgcaaa gctctggttt gcgtttagta gcaaggcttc ctgaaagaat tccggaggaa
     1321 aactccccct aactcacctt ttactctatg catctgctta attttgtgca attgtagtgc
     1381 aaatagtagg aaatgaattt tttttccgcc attttttcct tttcctattt ctgcttttca
     1441 atttccacgt ggagctcttt taaaatgaaa atttaaactc tagatatttc acgttttaaa
     1501 aaaaaaaaat tttaattttc tatcggtttt acttttgacg aaaagggttc acagacattg
     1561 gaaataatca tcatttacta tatataaagc gcttattctg tgtgtccata gtttgtagtc
     1621 tctgtagtct ttgtagtctg tgaagttttg gcttctggag ggatagtgag ttggggtcag
     1681 tgtagggata tagtcggggt actgtagtgg tacaatagtg gtacggtagg aatactgtag
     1741 ggttacggta gtttcataaa aaattaattt tcagccccag aagtcggggg ccgcgccgaa
     1801 ggtgcctaga cctaagccta ggcctcagcc taagcctaat tacatcaaaa aagctatgac
     1861 ttttggatca ttttgacttc ttttctagaa cgcataatga aataattctt aatttcatcg
     1921 gctttttctc atcgttgctt agaagtattg tcttgaaaat tgatattttt agaaagtaca
     1981 catatttctc aatcctttta tttaaatttc tgaaagaaac tttgccacaa cggtgagaaa
     2041 agggtgcccg aaaaatgtgc atttaaatgt gaaaaatatc cgtggccgcg gcctaagaac
     2101 ccccgtcggc catgtggtgc aagtgcgctc tgttgcggat ttttatattt aattgcattt
     2161 tcgcatattc gaacgatttt cctgattttc cagccgaaat tgtccattaa aaagtggcaa
     2221 gtttgtacaa aaaagttgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
     2281 agattttgca taaaaaacag actacataat actgtaaaac acaacatatg cagtcactat
     2341 gaaccaacta cttagatggt attagtgacc tgtagaattc gagctctaga gctgcagggc
     2401 ggccgcgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     2461 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     2521 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     2581 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     2641 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     2701 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     2761 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     2821 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     2881 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     2941 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     3001 cgcctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     3061 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     3121 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     3181 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     3241 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     3301 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     3361 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     3421 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     3481 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3541 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3601 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3661 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3721 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3781 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3841 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3901 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3961 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     4021 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4081 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4141 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4201 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4261 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     4321 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     4381 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     4441 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     4501 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     4561 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     4621 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     4681 gcatggatgt tttcccagtc acgacgt
//
