LOCUS       sequence-319314-        3959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Three-fragment Gateway compatible promoter for expression in all 
            muscles of C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   El Mouridi S, Peng Y, Frokjaer-Jensen C
  TITLE     Characterizing a strong pan-muscular promoter (Pmlc-1) as a 
            fluorescent co-injection marker to select for single-copy 
            insertions.
  JOURNAL   MicroPubl Biol. 2020 Sep 3;2020. doi: 
            10.17912/micropub.biology.000302.
  PUBMED    32908967
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2020-09-3"; volume: "2020. doi"; pages: " 
            10.17912/micropub.biology.000302"
COMMENT     Sequence Label: pCFJ2113 - [4-1] - Promoter - Pmlc-1 (w ATG)
FEATURES             Location/Qualifiers
     source          1..3959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..18
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2..18
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    58..153
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1469..1593
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1665..1684)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1666..1684)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1689..1705)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1689..1705)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1807..1826)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1818..2627
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1885..1904)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2774..3362
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3263..3282
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3516..3533
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3692..3719)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3811..3897)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(3946..3959,1..9)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgtaaaacga cggccagtct taagctcggg cccgagttaa cgctaccatg gagctccaaa
       61 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
      121 tttttataat gccaactttg tatagaaaag ttgagcgttt ctgccagctg acgcattatc
      181 ttaaaagctg ttttcagata agagcactgg tagacagaac actcaataga aaatcagatg
      241 aaaaagtttg aaagttgagg aatcaaaaaa gttatccttg ttaatttagg atatgagtaa
      301 gaacctcttg tcacactaat tctgatggaa ggtttttttt tctgaatcgt ttctgaattg
      361 tctccaatga gagtatagtt cgaaacacaa tttttgtctg ttattcaatg acatcaaaat
      421 tattgatttc aaaaatagct gcaaatccta aaatcaaaaa tcactgcgta tgtcaaaact
      481 ttgaagattg tgtgaaaatc aatatttgct ctgtttcatc ctatcttaaa atacgcccga
      541 ttaattattt cattttaaca aaacttgaaa aaaaactata caaatagatt tcacaagtag
      601 ttgaaacaat tagtaggaaa attataattt ataccagaaa aatatatgtc aaaaaaaatt
      661 atcgttagtg aaatattata aacagatatt actggaacca tctgataaat tcagtatgtc
      721 ccatttagtc atgttatgaa aattaaaatt tataatcaag ttccaaaaca aaagagtttc
      781 agattttcaa gttttcaatt atcctgtttg ttggaaatgt tgaatttaaa ccttcctttc
      841 caactcaaaa caaattctga ttatgttgtg ccctgctcag atttaaagct cggatgatct
      901 attccctttc ccttattgca tcccctcatt tatccgatcg agcaactttt tcgatggcgg
      961 gtaagaggca tcgcgacgca agcaaggcga gagttttgcc catcttcttg gaaacctccg
     1021 acgggtgcct gatgttagca ccacgggggc ggtgcgcctc ttgtgtaaag ttgttgagat
     1081 ttgctcaaac agccctcccc tcgacgcggg gcccaacgag tgtaaaaggc agacaaaagc
     1141 gacggataat cttcagttag tctcagttca ctactcagta cagtccacaa aatggtgagt
     1201 tggatttttt aataagttat ttgaaaagca tgagtttctg tataggaata ggagtgcgtt
     1261 catatatatt ttgatttaac ctgtctatat tgtatttaaa aaattattta ttttgaatca
     1321 gaggttctat aatcagtttc atctttaaaa aatttctggt aaattaagaa cgtaacattt
     1381 agatttaggc tttgtttaaa aatcaagcaa ttaaatcgac tataaaattg tcaatatgtt
     1441 ctcaattttc cagtccaagg ccgccaagac aagtttgtac aaaaaagttg aacgagaaac
     1501 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
     1561 atactgtaaa acacaacata tgcagtcact atgaaccaac tacttagatg gtattagtga
     1621 cctgtagaat tcgagctcta gagctgcagg gcggccgcga tatcccctat agtgagtcgt
     1681 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     1741 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
     1801 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
     1861 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     1921 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     1981 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     2041 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     2101 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag
     2161 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     2221 gtaattgtcc ttttaacagc gatcgcgtat ttcgcctcgc tcaggcgcaa tcacgaatga
     2281 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     2341 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     2401 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     2461 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     2521 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     2581 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     2641 attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac
     2701 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     2761 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2821 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2881 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2941 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3001 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3061 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3121 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3181 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3241 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3301 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3361 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3421 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3481 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3541 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     3601 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     3661 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     3721 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     3781 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     3841 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     3901 cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgt
//
