LOCUS       sequence-319315-        3873 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Three-fragment Gateway compatible promoter for expression in all 
            muscles of C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   El Mouridi S, Peng Y, Frokjaer-Jensen C
  TITLE     Characterizing a strong pan-muscular promoter (Pmlc-1) as a 
            fluorescent co-injection marker to select for single-copy 
            insertions.
  JOURNAL   MicroPubl Biol. 2020 Sep 3;2020. doi: 
            10.17912/micropub.biology.000302.
  PUBMED    32908967
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2020-09-3"; volume: "2020. doi"; pages: " 
            10.17912/micropub.biology.000302"
COMMENT     Sequence Label: pSEM223 - [4-1] - Promoter - Pmlc-2(no_start)
FEATURES             Location/Qualifiers
     source          1..3873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1919..2042
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2114..2133)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2115..2133)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2138..2154)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2138..2154)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2256..2275)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2267..3076
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2334..2353)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3223..3811
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3712..3731
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgtc acaagttcag attagctgtc gcctctaaaa
      721 atcaattgtt gaacaatgtc ttcttcaatt ttttgataat cagcaagcac aactactaga
      781 aacaaaatat acaattttaa agttggcaaa gaaacattgg atacaatttt ccagtcgctt
      841 ggcatttttg ttgaacataa tttcaatttt tcgaaaaatt tatgttttgt aatgttttat
      901 tttgatttcc atacttatga agtagtaaag atttctactg ggctcatgag atacaaaaca
      961 atctgaacat tcaatttttt atcttcggaa aagttcttgc tagaaaaaca ataaaaacca
     1021 cttataaaca ttcctttcaa ttgttctctt tttacgaact tgttaatttg tcttcagcca
     1081 ttagaaacaa tattcgatgt tacatgtttt cattcaacat tcaaagttat attctttcta
     1141 attcctattt tgtgttaatg ggccaccctc agtgcaactc tgatcattca tctcccaaaa
     1201 atccctagat tccctgctct ccaccctcgc ttttctgcgc gcagcaaaaa gcctaccacc
     1261 ttaactttta cctgcttttg agagagatgt acttgagaaa atattcgaag ccaaacagtt
     1321 ggcttttcca aaaattctct cccccatgcg ttgccgcaac tctgtagata tttgcgtttg
     1381 tcgtttcttc acaaacagat attgcgtata tcttcccggc cgtgtgaggg tgatcagaaa
     1441 gtctgagatc cccaaagaga gcacagacac ggcgcacgga tcacgggcac tgctctttct
     1501 agcgctcttt gtgtcgagct tctgcttggg acacattatc tctgctggca aaactgatcg
     1561 ctgcccgctg tgttacactc tatctcaaac ggcatcccgg gaatctgcca ccctccacca
     1621 attgcctact aactttgccc gccgtggaga gagcacctcc tctcggtctc ttttcgtatc
     1681 acattcctcc catccagcta ctcctccttc ccatacgcct accaaccaac cattattatc
     1741 tcaatgctct cgacaagatc gggacttgga aaaggcttct tcttcatact tttcatgggt
     1801 gcatttttaa ttttaatttg ttcgtttccc aagcaattta cttctatcat tgtttcgaca
     1861 attttgtgtg ctacctgtaa acaaataact gagtttcagg tagagaacct tttccaacca
     1921 agtttgtaca aaaaagttga acgagaaacg taaaatgata taaatatcaa tatattaaat
     1981 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat gcagtcacta
     2041 tgaaccaact acttagatgg tattagtgac ctgtagaatt cgagctctag agctgcaggg
     2101 cggccgcgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     2161 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     2221 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     2281 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     2341 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc
     2401 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     2461 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     2521 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt
     2581 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     2641 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     2701 tcgcctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     2761 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc
     2821 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     2881 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     2941 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     3001 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     3061 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct
     3121 gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     3181 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3241 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3301 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3361 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3421 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3481 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3541 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3601 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3661 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3721 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3781 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3841 cctggccttt tgctggcctt ttgctcacat gtt
//
