LOCUS       sequence-319328-       10436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration Vector, assembled with pHIE049 (TUPV1), pJM451 (TUPV2), 
            pPD612.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10436)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 10436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pHIE065 ZF43x12-S mKate2 EF1a EBFP2-P2A-BlastR
FEATURES             Location/Qualifiers
     source          1..10436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..600
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1..20)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     497..517
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(713..732)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    778..833
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(832..851)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     regulatory      2302..2311
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2308..3006
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    3057..3281
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        4330..5512
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4561..5503
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5460..5480
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     5514..5538
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5613..5622
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5619..5621,5622..5624,5625..6335)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(5664..5685)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             6363..6419
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6432..6830
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6881..7105
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(7964..8773)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8687..8706
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8765..8784
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(8932..9520)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9012..9031)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    10389..10426
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
ORIGIN
        1 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta
       61 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac
      121 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
      181 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
      241 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
      301 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
      361 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
      421 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
      481 gagacatccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
      541 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
      601 gcttccactg gattgtacaa ttaccaactg aattcactcc tcaggtgcag gctgcctatc
      661 agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc
      721 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
      781 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
      841 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
      901 caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt
      961 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
     1021 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
     1081 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
     1141 ctcttctctt atggagatcc gggcctagca tcgcgataag ctctagaggg acagcccccc
     1201 cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc
     1261 ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga
     1321 cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc
     1381 tttgagcctg cagacacctg gggggatacg gggaaaaagc tttaggctga aagagagatt
     1441 tagaatgaca gaatcataga acggcctgcg tacggatggg gctctagctc tagagcttga
     1501 tatcgaattc ctgcagcccc gctagaggga cagccccccc ccaaagcccc cagggatgta
     1561 attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg ctccggtccg
     1621 gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt
     1681 ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg
     1741 ggggatacgg ggaaaaagct ttaggctgaa agagagattt agaatgacag aatcatagaa
     1801 cggcctgggc gcgcctggtg gcgaacgatc aacagacggg agattgccaa ggtcttgaca
     1861 aacgagtgct agtacaagat ctgaattcgg atccagagtg aggactacct atagaagtaa
     1921 gagtgaggac aatcgtatac aagtagatcc agagtgagga ctacctatag aagtaagagt
     1981 gaggacaatc gtatacaagt agatccagag tgaggactac ctatagaagt aagagtgagg
     2041 acaatcgtat acaagtagat ccctctagag ggagatccag agtgaggact acctatagaa
     2101 gtaagagtga ggacaatcgt atacaagtag atccagagtg aggactacct atagaagtaa
     2161 gagtgaggac aatcgtatac aagtagatcc agagtgagga ctacctatag aagtaagagt
     2221 gaggacaatc gtatacaagt agatccctct agagggtata taatgggggc cactagtcta
     2281 ctaccagagc tcatcgctag cgccaccatg gtgagcgagc tgattaagga gaacatgcac
     2341 atgaagctgt atatggaggg caccgtgaac aaccaccact tcaagtgtac ctccgagggc
     2401 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggcggtcga gggcggccct
     2461 ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcaa aaccttcatc
     2521 aaccacaccc agggcatccc cgacttcttt aagcagtcct tccccgaggg cttcacatgg
     2581 gagagagtca ccacatacga agatgggggc gtgctgaccg ctacccagga caccagcctc
     2641 caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttccc atccaacggc
     2701 cctgtgatgc agaagaaaac actcggctgg gaggcctcca ccgaaaccct gtaccccgct
     2761 gacggcggcc tggaaggcag agccgacatg gccctgaagc tcgtgggcgg gggccacctg
     2821 atctgcaact tgaaaaccac atacagatcc aagaaacccg ctaagaacct caagatgccc
     2881 ggcgtctact atgtggacag gagactggaa agaatcaagg aggccgacaa agaaacctac
     2941 gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac
     3001 agatgagcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
     3061 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     3121 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     3181 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     3241 agtcaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg caggtcgagg
     3301 gtacatgcgc cttactcctt gtgagcgcat cgcgataagc tctagaggga cagccccccc
     3361 ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc
     3421 cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac
     3481 agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct
     3541 ttgagcctgc agacacctgg ggggatacgg ggaaaaagct ttaggctgaa agagagattt
     3601 agaatgacag aatcatagaa cggcctgcgt acggatgggg ctctagctct agagcttgat
     3661 atcgaattcc tgcagccccg ctagagggac agcccccccc caaagccccc agggatgtaa
     3721 ttacgtccct cccccgctag ggggcagcag cgagccgccc ggggctccgc tccggtccgg
     3781 cgctcccccc gcatccccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg
     3841 gcacgggatc gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg
     3901 gggatacggg gaaaaagctt taggctgaaa gagagattta gaatgacaga atcatagaac
     3961 ggcctgggcg cgcctggtgg cgaacgatca acagacggga gagggcaaaa ccgatgttga
     4021 cggactaatc ctgacaagat ctggggaggt cgctgagtag tgcgcgagca aaatttaagc
     4081 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     4141 tgcgctgctt cgcgatgtac gggccagata tacgcgtgag taattcatac aaaaggactc
     4201 gcccctgcct tggggaatcc cagggaccgt cgttaaactc ccactaacgt agaacccaga
     4261 gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc atcactgagg tggagaagag
     4321 catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     4381 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     4441 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     4501 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     4561 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     4621 ccttgaatta cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     4681 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     4741 agttgaggcc tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     4801 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     4861 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     4921 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     4981 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     5041 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     5101 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     5161 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     5221 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     5281 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     5341 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     5401 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     5461 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gagagctcgt
     5521 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     5581 gaacgggacc gatccaaatt ctcgaggcta gcgccaccat ggtgagcaag ggcgaggagc
     5641 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     5701 tcagcgtgag gggcgagggc gagggcgatg ccaccaacgg caagctgacc ctgaagttca
     5761 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgagccacg
     5821 gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     5881 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcacctaca
     5941 agacccgcgc cgaggtgaag ttcgagggcg acaccctagt gaaccgcatc gagctgaagg
     6001 gcgtcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aacttcaaca
     6061 gccacaacat ctatatcatg gccgtcaagc agaagaacgg catcaaggtg aacttcaaga
     6121 tccgccacaa cgtggaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     6181 ccatcggcga cggccccgtg ctgctgcccg acagccacta cctgagcacc cagtccgtgc
     6241 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc cgcaccgccg
     6301 ccgggatcac tctcggcatg gacgagctgt acaaggaatt cggtagcaag cttggtagtg
     6361 gagcaactaa cttttccctg ctcaaacaag ccggggatgt agaggagaac cccggccccg
     6421 ccccccttaa gatggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa
     6481 cggctacaat caacagcatc cccatctctg aggactacag cgtcgccagc gcagctctct
     6541 ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg
     6601 cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg
     6661 tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc
     6721 ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg
     6781 cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcggccgctc
     6841 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     6901 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     6961 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     7021 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagtcaa tagcaggcat
     7081 gctggggatg cggtgggctc tatggcttct gaggcaggtc gaagaactat ttcctggctg
     7141 ttacgcgagc gcatcgcgat aagctctaga gggacagccc ccccccaaag cccccaggga
     7201 tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg
     7261 tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga
     7321 aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac
     7381 ctggggggat acggggaaaa agctttaggc tgaaagagag atttagaatg acagaatcat
     7441 agaacggcct gcgtacggat ggggctctag ctctagagct tgatatcgaa ttcctgcagc
     7501 cccgctagag ggacagcccc cccccaaagc ccccagggat gtaattacgt ccctcccccg
     7561 ctagggggca gcagcgagcc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc
     7621 ccgagccggc agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg gatcgctttc
     7681 ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc tggggggata cggggaaaaa
     7741 gctttaggct gaaagagaga tttagaatga cagaatcata gaacggcctg ggcgcgcctg
     7801 gtggcgaacg atcaacagac gggagaggac tagaggatgc acatgtgacc gagggatgga
     7861 acgacgcgta actcacgtta agggattttg gtcatgagct tgcgccgtcc cgtcaagtca
     7921 gcgtaatgct ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga
     7981 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
     8041 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
     8101 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
     8161 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
     8221 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
     8281 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa
     8341 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
     8401 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
     8461 atgctgtttt tccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
     8521 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
     8581 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
     8641 gcttcccata caagcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
     8701 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctcgac gtttcccgtt
     8761 gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc
     8821 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acgggccatg
     8881 ctagctccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8941 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9001 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     9061 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     9121 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9181 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9241 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9301 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9361 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9421 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9481 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9541 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9601 atcaaaaagg atcttcacct agatcctttc tcgagctttc ggagtgagat ggagtggcca
     9661 ctgtggaact gggcctagca tcgcgataag ctctagaggg acagcccccc cccaaagccc
     9721 ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc
     9781 gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc
     9841 acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg
     9901 cagacacctg gggggatacg gggaaaaagc tttaggctga aagagagatt tagaatgaca
     9961 gaatcataga acggcctgcg tacggatggg gctctagctc tagagcttga tatcgaattc
    10021 ctgcagcccc gctagaggga cagccccccc ccaaagcccc cagggatgta attacgtccc
    10081 tcccccgcta gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc
    10141 cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat
    10201 cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg
    10261 ggaaaaagct ttaggctgaa agagagattt agaatgacag aatcatagaa cggcctgggc
    10321 gcgcctggtg gcgaacgatc aacagacggg agatctacta ttgggacagc agagatccag
    10381 ttggtaatgg cttgtcgacg acggcggtct ccgtcgtcag gatcataggt gccacc
//
