LOCUS       sequence-319331-        9665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses IntCCCre in mammalian cells in split intein-mediated 
            split-Cre recombinase applications.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Khoo ATT, Kim PJ, Kim HM, Je HS
  TITLE     Neural circuit analysis using a novel intersectional split 
            intein-mediated split-Cre recombinase system.
  JOURNAL   Mol Brain. 2020 Jul 2;13(1):101. doi: 10.1186/s13041-020-00640-2.
  PUBMED    32616061
REFERENCE   2  (bases 1 to 9665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2020-07-2"; volume: "13(1):101. doi"; pages: " 
            10.1186/s13041-020-00640-2"
COMMENT     Sequence Label: pCDH-CAG-IntCCCre-T2A-CopGFP
FEATURES             Location/Qualifiers
     source          1..9665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     537..556
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(775..879)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     947..965
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1003..1025)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1125..1144
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1338..1360
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1352..1369
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1353..1369
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1384..1610
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1611..1791
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1838..1963
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2456..2689
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2874..2918
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3185..3301
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     3357..3379
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3395..3411
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        3441..3820
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3822..4097
                     /label=chicken beta-actin promoter
     intron          4098..5114
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5038..5060
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5122..5141
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      5177..5186
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5186..5209
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6236..6259
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6269..6322
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6353..7015
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     misc_feature    7122..7710
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7175..7195)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7593..7604)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7784..8017
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8089..8210
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8126..8145)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8180..8199
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8250..8385
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8418..8434)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8418..8434)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8431..8453)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8442..8458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8466..8472,8473..8490,8491..8496))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8511..8532
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8649..8666)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8820..9408)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9579..9665,1..705,706..774))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
       61 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
      121 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
      181 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
      241 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
      301 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
      361 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
      421 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
      481 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
      541 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
      601 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
      661 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      721 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
      781 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
      841 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
      901 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
      961 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     1021 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     1081 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     1141 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     1201 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     1261 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     1321 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctgac
     1381 gcgtgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     1441 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     1501 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     1561 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     1621 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     1681 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     1741 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     1801 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     1861 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     1921 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     1981 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     2041 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     2101 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     2161 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     2221 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     2281 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
     2341 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     2401 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     2461 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     2521 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     2581 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     2641 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     2701 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     2761 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2821 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2881 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2941 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     3001 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     3061 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     3121 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt
     3181 taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     3241 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt
     3301 tatcgatgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     3361 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     3421 aggcgccgga attcgatgtc gacattgatt attgactagt tattaatagt aatcaattac
     3481 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     3541 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     3601 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     3661 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     3721 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     3781 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     3841 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     3901 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgccr ggsggggggg
     3961 ggsggggsgr ggggsggggs ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
     4021 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
     4081 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg
     4141 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
     4201 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
     4261 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
     4321 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc
     4381 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg
     4441 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag
     4501 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc
     4561 tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg
     4621 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg
     4681 gggtgccggg cggggcgggg ccgcctcggg ccggggrggg ctcgggggrg gggcgcggcg
     4741 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     4801 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     4861 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     4921 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     4981 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     5041 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
     5101 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     5161 aagaattctt aattaagcca ccatggatta taaagacgat gacgataaaa tggttaaagt
     5221 tatcggtcgt cgttccctcg gagtgcaaag aatatttgat attggtcttc gccaagacca
     5281 taattttctg ctagccaatg gggcgatcgc ggccaattgc ttcaataagg aggacggatc
     5341 caaccggaaa tggtttcccg cagaacctga agatgttcgc gattatcttc tatatcttca
     5401 ggcgcgcggt ctggcagtaa aaactatcca gcaacatttg ggccagctaa acatgcttca
     5461 tcgtcggtcc gggctgccac gaccaagtga cagcaatgct gtttcactgg ttatgcggcg
     5521 gatacgaaaa gaaaacgttg atgccggtga acgtgcaaaa caggctctag cgttcgaacg
     5581 cactgatttc gaccaggttc gttcactcat ggaaaatagc gatcgctgcc aggatatacg
     5641 taatctggca tttctgggga ttgcttataa caccctgtta cgtatagccg aaattgccag
     5701 gatcagggtt aaagatatct cacgtactga cggtgggaga atgttaatcc atattggcag
     5761 aacgaaaacg ctggttagca ccgcaggtgt agagaaggca cttagcctgg gggtaactaa
     5821 actggtcgag cgatggattt ccgtctctgg tgtagctgat gatccgaata actacctgtt
     5881 ttgccgggtc agaaaaaatg gtgttgccgc gccatctgcc accagccagc tatcaactcg
     5941 cgccctggaa gggatttttg aagcaactca tcgattgatt tacggcgcta aggatgactc
     6001 tggtcagaga tacctggcct ggtctggaca cagtgcccgt gtcggagccg cgcgagatat
     6061 ggcccgcgct ggagtttcaa taccggagat catgcaagct ggtggctgga ccaatgtaaa
     6121 tattgtcatg aactatatcc gtaacctgga tagtgaaaca ggggcaatgg tgcgcctgct
     6181 ggaagatggc gatgaattct gcagatatcc atcacactgg cggccgctcg agatggacta
     6241 caaggacgac gatgataagg gcaccggtga gggcagagga agtcttctaa catgcggtga
     6301 cgtggaggag aatcccggcc ctcccgggat ggagagcgac gagagcggcc tgcccgccat
     6361 ggagatcgag tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg
     6421 cggagagggc acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc
     6481 cctgaccttc agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg
     6541 cacctacccc agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac
     6601 caacacccgc atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg
     6661 ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga
     6721 cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc
     6781 catgggcgat aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg
     6841 ctactacagc ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat
     6901 cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac
     6961 cgagctgggc atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc
     7021 ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccctgta
     7081 caagtaacac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct ctggattaca
     7141 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     7201 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     7261 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7321 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7381 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7441 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7501 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     7561 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7621 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga
     7681 gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat gacttacaag
     7741 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7801 tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7861 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7921 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7981 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     8041 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     8101 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8161 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     8221 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     8281 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     8341 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag
     8401 accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     8461 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     8521 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     8581 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     8641 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8701 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8761 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8821 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8881 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8941 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9001 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9061 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9121 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9181 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9241 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9301 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9361 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9421 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9481 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     9541 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     9601 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9661 tagat
//
