LOCUS       sequence-319336-        9601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NCreIntN in mammalian cells in split intein-mediated 
            split-Cre recombinase applications.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   Khoo ATT, Kim PJ, Kim HM, Je HS
  TITLE     Neural circuit analysis using a novel intersectional split 
            intein-mediated split-Cre recombinase system.
  JOURNAL   Mol Brain. 2020 Jul 2;13(1):101. doi: 10.1186/s13041-020-00640-2.
  PUBMED    32616061
REFERENCE   2  (bases 1 to 9601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2020-07-2"; volume: "13(1):101. doi"; pages: " 
            10.1186/s13041-020-00640-2"
COMMENT     Sequence Label: pCDH-CAG-NCreIntN-EF1a-mTagBFP2
FEATURES             Location/Qualifiers
     source          1..9601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             34..214
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    261..386
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    879..1112
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1297..1341
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1608..1724
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1780..1802
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1818..1834
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1864..2243
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2245..2520
                     /label=chicken beta-actin promoter
     intron          2521..3537
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3461..3483
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3545..3564
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3607..3627
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3628..3654
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4203..4414
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4427..4695
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      4730..4739
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4736..5446
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    5481..6069
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5534..5554)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5952..5963)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6143..6376
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6448..6569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6485..6504)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6539..6558
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6609..6744
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6777..6793)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6777..6793)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6790..6812)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6801..6817
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6825..6831,6832..6849,6850..6855))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6870..6891
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7008..7025)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7179..7767)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7938..8729,8730..8798))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8561..8580
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8799..8903)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8971..8989
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9027..9049)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9149..9168
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9362..9384
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9376..9393
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9377..9393
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(9408..9601,1..33)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
       61 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      121 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      181 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      241 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      301 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      361 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      421 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      481 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      541 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
      601 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
      661 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
      721 aagcggccac tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
      781 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
      841 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
      901 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
      961 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1021 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1081 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1141 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1201 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1261 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1321 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1381 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1441 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1501 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1561 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
     1621 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     1681 caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgat gtacacccta
     1741 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga
     1801 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattcgat
     1861 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     1921 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     1981 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2041 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2101 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2161 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2221 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
     2281 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
     2341 cgatgggggc gggggggggg ggggsgcgcg ccaggcgggg cggggcgggg cgaggggcgg
     2401 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
     2461 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
     2521 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
     2581 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
     2641 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
     2701 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
     2761 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
     2821 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     2881 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     2941 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     3001 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     3061 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     3121 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     3181 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     3241 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     3301 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     3361 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     3421 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     3481 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     3541 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt cgctagcgcc
     3601 accatgccca agaagaagcg gaaggtgtac ccatacgatg ttccagatta cgcaggttct
     3661 ggttccaatt tactgaccgt acaccaaaat ttgcctgcat taccggtcga tgcaacgagt
     3721 gatgaggttc gcaagaacct gatggacatg ttcagggatc gccaggcgtt ttctgagcat
     3781 acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg catggtgcaa gttgaatgaa
     3841 ttccttaagg ggtgtttaag ctatgaaacg gaaatattga cagtagaata tggatcatta
     3901 ccgattggta aaattgtaga aaagcgcatc gaatgtactg tttatagtgt tgataataat
     3961 ggaaatattt atacacaacc tgtagcacaa tggcacgatc gcggagaaca agaggtgttt
     4021 gagtattgtt tggaagatgg ttcattgatt cgggcaacaa aagaccataa gtttatgact
     4081 gttgatggtc aaatgttgcc aattgatgaa atatttgaac gtgaattgga tttgatgcgg
     4141 gttgataatt tgccgaatta ggcggccgcg aaggatctgc gatcgctccg gtgcccgtca
     4201 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     4261 aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     4321 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     4381 tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc
     4441 tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct
     4501 gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct
     4561 caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct
     4621 ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct
     4681 gcgccgttac agatccaagc tgtgaccggc gcctacgcta gacgctagcg ccaccatggt
     4741 gtctaagggc gaagagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
     4801 cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
     4861 ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
     4921 ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga
     4981 cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga
     5041 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
     5101 cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact
     5161 cggctgggag gccttcaccg agacgctgta ccccgctgac ggcggcctgg aaggcagaaa
     5221 cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacgcca agaccacata
     5281 tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag
     5341 actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt
     5401 ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaatcacc tggtgcatga
     5461 cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa agattgactg
     5521 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5581 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5641 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5701 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5761 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5821 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5881 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5941 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6001 tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     6061 cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     6121 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag ataagatctg
     6181 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6241 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6301 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6361 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     6421 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     6481 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6541 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     6601 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6661 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     6721 agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt aatcatgtca
     6781 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6841 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6901 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6961 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7021 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7081 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7141 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7201 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7261 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7321 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7381 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7441 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7501 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7561 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7621 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7681 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7741 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7801 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7861 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7921 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7981 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8041 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8101 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8161 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8221 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8281 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8341 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8401 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8461 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8521 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8581 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8641 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8701 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8761 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8821 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8881 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     8941 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     9001 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     9061 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     9121 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     9181 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
     9241 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     9301 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     9361 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tgacgcgtgt agtcttatgc
     9421 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     9481 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     9541 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     9601 t
//
