LOCUS       sequence-319337-        9109 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NCreIntN in mammalian cells in split intein-mediated 
            split-Cre recombinase applications.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9109)
  AUTHORS   Khoo ATT, Kim PJ, Kim HM, Je HS
  TITLE     Neural circuit analysis using a novel intersectional split 
            intein-mediated split-Cre recombinase system.
  JOURNAL   Mol Brain. 2020 Jul 2;13(1):101. doi: 10.1186/s13041-020-00640-2.
  PUBMED    32616061
REFERENCE   2  (bases 1 to 9109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2020-07-2"; volume: "13(1):101. doi"; pages: " 
            10.1186/s13041-020-00640-2"
COMMENT     Sequence Label: pCDH-CAG-NCreIntN-T2A-mTagBFP2
FEATURES             Location/Qualifiers
     source          1..9109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     537..556
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(775..879)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     947..965
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1003..1025)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1125..1144
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1338..1360
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1352..1369
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1353..1369
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1384..1610
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1611..1791
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1838..1963
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2456..2689
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2874..2918
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3185..3301
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     3357..3379
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3395..3411
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        3441..3820
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3822..4097
                     /label=chicken beta-actin promoter
     intron          4098..5114
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5038..5060
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5122..5141
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             5186..5206
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5207..5233
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5747..5800
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5807..6517
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             5825..6523
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="ELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVE
                     GGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQ
                     DTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLV
                     GGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDL
                     PSKLGHKLNH"
     misc_feature    6566..7154
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6619..6639)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7037..7048)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7228..7461
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7533..7654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7570..7589)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7624..7643
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7694..7829
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7862..7878)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7862..7878)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7875..7897)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7886..7902
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7910..7916,7917..7934,7935..7940))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7955..7976
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8093..8110)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8264..8852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9023..9109,1..705,706..774))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
       61 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
      121 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
      181 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
      241 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
      301 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
      361 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
      421 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
      481 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
      541 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
      601 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
      661 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      721 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
      781 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
      841 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
      901 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
      961 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     1021 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     1081 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     1141 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     1201 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     1261 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     1321 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctgac
     1381 gcgtgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     1441 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     1501 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     1561 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     1621 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     1681 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     1741 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     1801 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     1861 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     1921 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     1981 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     2041 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     2101 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     2161 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     2221 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     2281 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
     2341 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     2401 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     2461 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     2521 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     2581 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     2641 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     2701 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     2761 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2821 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2881 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2941 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     3001 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     3061 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     3121 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt
     3181 taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     3241 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt
     3301 tatcgatgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     3361 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     3421 aggcgccgga attcgatgtc gacattgatt attgactagt tattaatagt aatcaattac
     3481 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     3541 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     3601 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     3661 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     3721 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     3781 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     3841 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     3901 ttaattattt tgtgcagcga tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg
     3961 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
     4021 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
     4081 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg
     4141 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
     4201 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
     4261 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
     4321 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc
     4381 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg
     4441 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag
     4501 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc
     4561 tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg
     4621 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg
     4681 gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg
     4741 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     4801 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     4861 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     4921 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     4981 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     5041 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
     5101 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     5161 aagaattcgc ggccgcgcca ccatgcccaa gaagaagcgg aaggtgtacc catacgatgt
     5221 tccagattac gcaggttctg gttccaattt actgaccgta caccaaaatt tgcctgcatt
     5281 accggtcgat gcaacgagtg atgaggttcg caagaacctg atggacatgt tcagggatcg
     5341 ccaggcgttt tctgagcata cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc
     5401 atggtgcaag ttgaatgaat tccttaaggg gtgtttaagc tatgaaacgg aaatattgac
     5461 agtagaatat ggatcattac cgattggtaa aattgtagaa aagcgcatcg aatgtactgt
     5521 ttatagtgtt gataataatg gaaatattta tacacaacct gtagcacaat ggcacgatcg
     5581 cggagaacaa gaggtgtttg agtattgttt ggaagatggt tcattgattc gggcaacaaa
     5641 agaccataag tttatgactg ttgatggtca aatgttgcca attgatgaaa tatttgaacg
     5701 tgaattggat ttgatgcggg ttgataattt gccgaattat gctagcgagg gcagaggaag
     5761 tcttctaaca tgcggtgacg tggaggagaa tcccggccct ggatccatgg tgtctaaggg
     5821 cgaagagctg attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa
     5881 ccatcacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat
     5941 gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag
     6001 cttcctctac ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa
     6061 gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt
     6121 gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat
     6181 cagaggggtg aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga
     6241 ggccttcacc gagacgctgt accccgctga cggcggcctg gaaggcagaa acgacatggc
     6301 cctgaagctc gtgggcggga gccatctgat cgcaaacgcc aagaccacat atagatccaa
     6361 gaaacccgct aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag
     6421 aatcaaggag gccaacaacg agacctacgt cgagcagcac gaggtggcag tggccagata
     6481 ctgcgacctc cctagcaaac tggggcacaa gcttaatcac tagggcgcgc ccacctggtg
     6541 catgacccgc aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt
     6601 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6661 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6721 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6781 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6841 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6901 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6961 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7021 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7081 ggctctgcgg cctcttccgc gtctccgcct tcgccctcag acgagtcgga tctccctttg
     7141 ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta
     7201 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagataag
     7261 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7321 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7381 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7441 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     7501 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     7561 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7621 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     7681 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7741 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7801 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca
     7861 tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7921 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7981 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8041 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8101 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8161 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8221 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8281 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8341 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8401 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8461 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8521 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8581 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8641 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8701 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8761 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8821 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8881 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8941 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9001 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9061 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagat
//
