LOCUS       sequence-319340-        9291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses PKD2L2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9291)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_PKD2L2_OE_1
FEATURES             Location/Qualifiers
     source          1..9291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        76..379
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        380..583
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     534..554
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    606..630
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2200..2224)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2226..2267
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2309..2897
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2362..2382)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2780..2791)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2969..3202
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3280..3401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3317..3336)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3371..3390
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3441..3576
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3596..3615)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3597..3615)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3625..3642)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3625..3641)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3634..3656)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3783..4238
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3870..3889)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4080..4101
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4264..4368
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4369..4437,4438..5229)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4587..4606)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5400..5988
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5889..5908
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6142..6159
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6276..6297
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6312..6317,6318..6335,6336..6342)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6350..6366
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6355..6377
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6374..6390
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6374..6390
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6409..6429
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6411..6429
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6457..6683
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6684..6864
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6911..7036
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7529..7762
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7947..7991
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8166..8666
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8585..8603
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8678..9073
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9133..9250
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gctcgagggg gcccggggtt attaatagta atcaattacg gggtcattag ttcatagccc
       61 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      121 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      181 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      241 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      301 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      361 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      421 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      481 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      541 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactgtcgg
      601 gatcaacaag tttgtacaaa aaagttggca tggctgaggc gtcacggtgg caccgaggcg
      661 gggcttcgaa acataagttg cattacagaa aggaagtaga aattacaacc acacttcagg
      721 aattgttact ctactttatt tttttaataa acctatgtat attgactttt gggatggtaa
      781 acccacatat gtattactta aacaaggtta tgtcatctct atttttggac acttctgtgc
      841 ctggtgaaga aagaaccaac tttaagtcca ttcgcagcat aactgatttt tggaagttta
      901 tggaaggacc ccttttggaa ggtctgtact gggattcatg gtacaataac cagcagctgt
      961 ataatttaaa gaacagcagt cgcatctact atgaaaatat acttctagga gttcccagag
     1021 ttcgtcaact aaaagtccgc aacaacacat gcaaagtcta ttcatctttt cagtctttga
     1081 tgagtgaatg ttatggcaaa tatacttctg caaatgaaga cctctctaat tttggccttc
     1141 aaattaatac tgaatggaga tattctactt ctaataccaa ctccccttgg cactggggat
     1201 ttcttggtgt ttaccgaaat gggggataca ttttcacttt atcaaaatcg aaatctgaaa
     1261 ccaaaaacaa gttcattgac cttcgactga acagctggat cacaagaggg actagagtta
     1321 tttttattga tttttcctta tataatgcta atgtaaatct attttgtatt atcagattgg
     1381 tggcagaatt ccctgcaact ggaggaatac ttacttcatg gcagttttac tctgtgaagc
     1441 tcctcagata tgttagctac tatgactatt ttattgcttc ctgtgaaatc acattctgta
     1501 tttttctttt tgtcttcaca acacaagaag tcaaaaaaat aaaagaattt aagtctgcct
     1561 atttcaaaag tatttggaac tggctagaat tgctactttt gctgttgtgt tttgtggctg
     1621 tttccttcaa cacatactat aatgtacaaa tttttctctt acttggacag ctgttgaaaa
     1681 gtactgaaaa atattcagat ttctattttc ttgcatgctg gcacatttat tacaataata
     1741 taattgctat taccatcttt tttgcatgga taaagaatat gttcttggca attattaatg
     1801 atacctattc tgaagtgaaa gctgactatt caataggcag aaggccagat tttgaacttg
     1861 gcaaaatgat taaacagagt tacaaaaatg ttctcgagaa attcagactg aagaaagctc
     1921 aaaaagatga agacaagaaa accaaaggca gcggagattt ggctgaacaa gccagaagag
     1981 aaggctttga cgaaaatgag attcaaaacg cagagcagat gaaaaaatgg aaagagaggc
     2041 ttgagaaaaa gtattattct atggaaattc aagatgacta ccagcctgtc actcaagaag
     2101 aatttcgaga acttttttta tatgctgtgg agctggagaa ggaattacac tacatcaatt
     2161 tgaagctaaa tcaagtggtg agaaaggttt cagctctata cccaactttc ttgtacaaag
     2221 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
     2281 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     2341 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     2401 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     2461 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     2521 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     2581 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     2641 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     2701 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     2761 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     2821 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     2881 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     2941 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     3001 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     3061 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     3121 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     3181 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
     3241 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     3301 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3361 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     3421 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3481 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     3541 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     3601 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     3661 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     3721 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     3781 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3841 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3901 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3961 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     4021 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4081 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     4141 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4201 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     4261 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4321 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4381 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4441 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4501 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4561 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4621 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4681 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4741 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4801 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4861 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4921 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4981 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5041 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5101 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5161 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5221 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5281 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5341 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5401 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5461 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5521 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5581 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5641 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5701 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5761 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5821 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5881 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5941 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6001 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6061 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     6121 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     6181 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     6241 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     6301 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     6361 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     6421 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     6481 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     6541 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     6601 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     6661 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     6721 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6781 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6841 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     6901 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     6961 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     7021 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     7081 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     7141 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     7201 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     7261 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     7321 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     7381 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     7441 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     7501 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     7561 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     7621 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     7681 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     7741 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     7801 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     7861 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     7921 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     7981 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     8041 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     8101 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     8161 gacccttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
     8221 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
     8281 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
     8341 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
     8401 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
     8461 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
     8521 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg
     8581 cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg
     8641 accgaatcac cgacctctct ccccaggggg taccaccatg gccaagcctt tgtctcaaga
     8701 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga
     8761 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt
     8821 atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc
     8881 ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag
     8941 cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt
     9001 gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta
     9061 tgtgtgggag ggcctgcagc tgcagtagta agaattctag atcttgagac aaatggcagt
     9121 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     9181 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     9241 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc g
//
