LOCUS       sequence-319342-        2366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term 8xHis uncleavable Spec resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 27 pCOLI_S_C_HIS
FEATURES             Location/Qualifiers
     source          1..2366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     696..715
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        696..714
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             752..774
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             834..857
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(951..969)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      965..1012
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1183..1974)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1975..2066)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(2114..2366,1..486))
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 gctctcctgt tccgaccctg ccgtttaccg gatacctgtt ccgcctttct cccttacggg
       61 aagtgtggcg ctttctcata gctcacacac tggtatctcg gctcggtgta ggtcgttcgc
      121 tccaagctgg gctgtaagca agaactcccc gttcagcccg actgctgcgc cttatccggt
      181 aactgttcac ttgagtccaa cccggaaaag cacggtaaaa cgccactggc agcagccatt
      241 ggtaactggg agttcgcaga ggatttgttt agctaaacac gcggttgctc ttgaagtgtg
      301 cgccaaagtc cggctacact ggaaggacag atttggttgc tgtgctctgc gaaagccagt
      361 taccacggtt aagcagttss ssaactgnct taaccttcga tcaaaccacc tccccaggtg
      421 gttttttcgw ttacagggca aaagattacg cgcagaaaaa aaggatctca agaagatcct
      481 ttgatctttt ctactgaacc gctctagatt tcagtgcaat ttatctcttc aaatgtagca
      541 cctgaagtca gccccatacg atataagttg taattctcat gttagtcatg ccccgcgccc
      601 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
      661 aatgagtgag ctaacttaca ttaattgcgt tgcgctaata cgactcacta tagggagacc
      721 acaacggttt ccctccggct ccggaaataa ttttgtttaa ctttaagaag gagactggat
      781 ccgaatctag agcctgcagt ctcgacaagc ttggaagcgg atctggatct ggacatcatc
      841 atcatcatca ccatcactaa taagtcgaga agtactagag gatgtacaac gcgtgcgcgc
      901 tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      961 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaacc
     1021 tcaggcattt gagaagcaca cggtcacact gcttccggta gtcaataaac cggtaaacca
     1081 gcaatagaca taagcggcta tttaacgacc ctgccctgaa ccgacgaccg ggtcatcgtg
     1141 gccggatctt gcggcccctc ggcttgaacg aattgttaga cattatttgc cgactacctt
     1201 ggtgatctcg cctttcacgt agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa
     1261 gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg
     1321 ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt
     1381 tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca
     1441 gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc
     1501 aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct
     1561 tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc
     1621 aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca
     1681 cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc
     1741 tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc
     1801 aagccttacg gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc
     1861 cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac
     1921 gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatactctt
     1981 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     2041 tgaatgtatt tagaaaaata aacaaatagc tagctcactc ggtcgctacg ctccgggcgt
     2101 gagactgcgg cgggcgctgc ggacacatac aaagttaccc acagattccg tggataagca
     2161 ggggactaac atgtgaggca aaacagcagg gccgcgccgg tggcgttttt ccataggctc
     2221 cgccctcctg ccagagttca cataaacaga cgcttttccg gtgcatctgt gggagccgtg
     2281 aggctcaacc atgaatctga cagtacgggc gaaacccgac aggacttaaa gatccccacc
     2341 gtttccggcg ggtcgctccc tcttgc
//
