LOCUS       sequence-319344-        9027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses CACNG8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9027)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_CACNG8_OE_1
FEATURES             Location/Qualifiers
     source          1..9027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        34..337
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        338..541
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     492..512
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    564..588
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1894..1918)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1920..1961
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2003..2591
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2056..2076)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2474..2485)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2663..2896
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2974..3095
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3011..3030)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3065..3084
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3135..3270
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3290..3309)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3291..3309)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3319..3336)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3319..3335)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3328..3350)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3477..3932
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3564..3583)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3774..3795
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3958..4062
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4063..4131,4132..4923)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4281..4300)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5094..5682
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5583..5602
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5836..5853
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5970..5991
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6006..6011,6012..6029,6030..6036)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6044..6060
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6049..6071
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6068..6084
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6068..6084
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6103..6123
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6105..6123
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6151..6377
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6378..6558
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6605..6730
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7223..7456
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7641..7685
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7860..8360
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8279..8297
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8372..8767
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8827..8944
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
       61 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      121 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      181 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      241 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      301 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
      361 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      421 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
      481 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      541 tctctggcta actgtcggga tcaacaagtt tgtacaaaaa agcagaaggg ccgtcaaggc
      601 ccaccctgga gtcgctgaag cgctggaacg aagagcgggg cctctggtgc gagaaggggg
      661 tgcaggtgct gctgacgacg gtgggcgcct tcgccgcctt cggcctcatg accatcgcca
      721 tcagcactga ctactggctc tacacgcgcg ccctcatctg caacaccacc aacctcacgg
      781 ccggcggcga cgacgggacc ccccaccgcg ggggcggcgg cgcctcggag aagaaggacc
      841 ccggcggcct cacgcactcg ggcctctgga ggatctgctg cctggaaggg ttgaaaagag
      901 gcgtctgcgt gaagatcaat catttcccgg aggacacgga ctacgaccac gacagcgcgg
      961 agtatctact ccgagttgtc cgggcctcca gcatcttccc catccttagc gccatcctgc
     1021 tgctgctcgg gggtgtgtgc gtggcggcct cccgcgtcta caagtccaag aggaacatca
     1081 ttctgggcgc agggatcctg ttcgtggcag caggcctgag caacatcatc ggcgtgatcg
     1141 tgtacatctc cgccaacgcg ggcgagccgg gcccgaagcg ggacgaggag aagaaaaacc
     1201 actactcgta cggctggtcc ttctacttcg gcgggctgtc gttcatcctg gccgaggtga
     1261 taggcgtgct ggccgtcaac atctacatcg agcgcagccg cgaggcgcac tgccagtctc
     1321 gctcggacct gctcaaggcc ggcgggggcg cgggcggcag tggcgggagc ggcccctcgg
     1381 ccatcctccg tctgcccagt taccgcttcc gctaccgccg ccgctcccgc tctagctccc
     1441 gctccagcga gccgtcgccg tcgcgggacg cgtctcccgg cggccccggg ggcccgggct
     1501 ttgcctccac ggacatctcc atgtacacgc tcagccgcga cccctccaag ggcagcgtgg
     1561 ccgcggggct ggcgggggcc ggcggcggcg gcggcggcgc cgtgggggcg ttcggcggcg
     1621 cggccggggg cgccgggggc ggcggcggag gcggcggcgg ggcgggtgcc gagcgggacc
     1681 gcgggggggc gtccggcttc ctcacgctgc acaacgcctt ccccaaggag gcgggcggcg
     1741 gcgtcacggt cacggtcacc gggccgcccg ccccgcccgc gcccgcgcca cccgcgccct
     1801 ctgcgcccgc ccccgggacc ctggccaagg aggccgccgc ctccaacacc aacacgctca
     1861 acaggaaaac cacgcctgtg tcaggcctca tgggcccagc tttcttgtac aaagtggttg
     1921 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
     1981 accggtggcg cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     2041 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     2101 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     2161 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     2221 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     2281 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     2341 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     2401 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     2461 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     2521 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     2581 cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     2641 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     2701 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     2761 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     2821 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     2881 tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa
     2941 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     3001 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3061 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     3121 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     3181 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     3241 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     3301 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     3361 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     3421 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     3481 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     3541 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     3601 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     3661 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     3721 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     3781 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     3841 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     3901 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     3961 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4021 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4081 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4141 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4201 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4261 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     4321 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     4381 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4441 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4501 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4561 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4621 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4681 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4741 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     4801 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4861 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4921 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     4981 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5041 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5101 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5161 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5221 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     5281 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5341 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5401 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5461 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5521 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5581 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5641 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5701 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     5761 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     5821 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     5881 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     5941 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     6001 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     6061 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     6121 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     6181 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     6241 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     6301 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     6361 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6421 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6481 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6541 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     6601 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     6661 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     6721 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     6781 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     6841 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     6901 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     6961 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     7021 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     7081 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     7141 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     7201 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     7261 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     7321 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     7381 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     7441 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     7501 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     7561 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     7621 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     7681 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     7741 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     7801 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccct
     7861 tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt
     7921 ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg
     7981 tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc
     8041 taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac
     8101 gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc
     8161 gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag
     8221 gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt
     8281 gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa
     8341 tcaccgacct ctctccccag ggggtaccac catggccaag cctttgtctc aagaagaatc
     8401 caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag
     8461 cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca
     8521 ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc
     8581 tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg
     8641 cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga
     8701 cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg
     8761 ggagggcctg cagctgcagt agtaagaatt ctagatcttg agacaaatgg cagtattcat
     8821 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     8881 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     8941 ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg agggggcccg
     9001 gggttattaa tagtaatcaa ttacggg
//
