LOCUS       sequence-319350-        2792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and mEGFP-linker sequence sequence for N-terminus 
            tagging of human POLR2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2792)
  TITLE     Allen Institute for Cell Sciences Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-117:POLR2A-mEGFP
FEATURES             Location/Qualifiers
     source          1..2792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(998..1000,1001..1003,1004..1714)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1043..1064)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1304..1323)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1651..1672
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aagtctcttc gagaggctca ggccatggat ctaggatcat ggctcggtac agcgcggcat
       61 tacaggccag gagatgccca cccgtctccg ggggccgttt gacaaacatc ggctacttcc
      121 ggacaagcca ctggtcctgc catcttggtt tgttcatgca tattcagcaa gagaactgca
      181 tattcatgag caaccgccct tccccgggag gacccgcccc gccccgccgc cattaccgat
      241 tcgcagagca gaccattttc cagctggggg gggctacgaa tgagaggctt ttgctgaaga
      301 tgaaaccgtt gtccaaacta gaatgaaagc ggcccaattt taccctcgag tcttcaaacc
      361 ttaagcctca gtgcaaaagg gagcagcgag gagaggcggt gggattaaag aaaaaaattc
      421 ttctctccct gtcacttcaa aaggtcgccc aaccagcgag cggggggttt acccacgact
      481 ctggctcgca ccccttgcgg gcctgcgcag gcggccccgt agcgcaaggg agggcgggaa
      541 aggaaggggc ggggacgcga gggcgaatct ataagaagcg tcgttcagcg agttcgctgc
      601 tcagaagcgc cgagagcgcg gccgggacgg ttggagaaga aggcggctcc cggaaggggg
      661 agagacaaac tgccgtaacc tctgccgttc aggaacccgg ttacttattt attcgttacc
      721 ctttttcttc ttcctccccc aaaaaccttt tccttttccc ttcttttttt ttcctttttg
      781 ggagctgaaa aatttccggt aagggaaaga agggctcctt tcgctcctta tttccccgcc
      841 tctttccctc ccccaccttc ccctcctccg gctttttcct cccaactcgg ggaggtcctt
      901 cccggtggcc gccctgacga ggtctgagca cctaggcgga ggcggcgcag gctttttgta
      961 gtgaggtttg cgcctgcgca gcgcgcctgc ctccgccatg gtgagcaagg gcgaggagct
     1021 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1081 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1141 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1201 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1261 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1321 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1381 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1441 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1501 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1561 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     1621 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1681 cgggatcact ctcggcatgg acgagctgta caagagcggc ctgagaagca gagcccaggc
     1741 ctctaactcc gccgtcgacg gcaccgctgg acccggaagc acaggcagct cccacggggg
     1801 tggacccccc tcgggggaca gcgcatgccc gctgcgcacc atcaagagag tccagttcgg
     1861 agtcctgagt ccggatgaac tggtaagcgg ctctgtcctc cccttccccc ctcctcccct
     1921 ggcgggcggg gccggacggg ggctgcggaa acttggcgct ttctcgctgc ttatgggtga
     1981 cgggccagga gcatggctca gcagcgccaa ggccgtgtag gcgccagtct cgggcctccc
     2041 agagttatat tttgcaaaga gttgagcagg cgtagcgctt tgtccgagat ggggggctgg
     2101 ctggagggtg agaaggaggg agagaaggat gactgatttc acactccaga gttaccgacg
     2161 ttaaaggcga tccgacgaat cctagggagt ctttacaagt ttggttaaga agccaaaccc
     2221 gggtagcttc ctcctcttct gtaatacaaa tgttaactga gcacctgctc tgttgaagac
     2281 acaggagact cgggttacag aggtgaacac gacagaccag gaccctacct tcaagagcac
     2341 tgtctgtggg aaagggcaag cagtaaacat gaatacaatg aatgaatgag ataattcagt
     2401 taatggcatt tgtctcgaat agagtgaaaa ggggtaatcc catggagagt cactgagctg
     2461 gggctacttt atttttttat ttttatttta ttttttgaga cggagtcttg ctctgtcgcc
     2521 caggctggag tgcaatggca cgatctcggc tcactgcaat ctccgcctcc cgggttcaag
     2581 cgattctcct gccttagcct cctgagtagc tgggattaca ggcatgcgcc accatgcccc
     2641 gctaattttt gtatttttag tagaggaggg gttttgccat gttggccagg ctggtctcga
     2701 acgcctgccc tgaggtgatt ctcccccgtc tttttttttt tttttttttt tttgagacgg
     2761 agtttcgctc ttattcccca ggccagagtg ca
//
