LOCUS       sequence-319354-        2561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term HA 3C cleavable Spec resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 32 pCOLI_S_HA
FEATURES             Location/Qualifiers
     source          1..2561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(128..219)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(267..1006)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     primer_bind     1216..1235
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1216..1234
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1272..1294
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1314..1331
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1344..1370
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1377..1403
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1410..1436
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1449..1472
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(1665..1683)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1679..1726
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(join(1897..2561,1..127))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat
       61 ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt
      121 ccctcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
      181 gcggatacat atttgaatgt atttagaaaa ataaacaaat agctagctca ctcggtcgct
      241 acgctccggg cgtgagactg cggcgggcgc tgcggacaca tacaaagtta cccacagatt
      301 ccgtggataa gcaggggact aacatgtgag gcaaaacagc agggccgcgc cggtggcgtt
      361 tttccatagg ctccgccctc ctgccagagt tcacataaac agacgctttt ccggtgcatc
      421 tgtgggagcc gtgaggctca accatgaatc tgacagtacg ggcgaaaccc gacaggactt
      481 aaagatcccc accgtttccg gcgggtcgct ccctcttgcg ctctcctgtt ccgaccctgc
      541 cgtttaccgg atacctgttc cgcctttctc ccttacggga agtgtggcgc tttctcatag
      601 ctcacacact ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa
      661 gaactccccg ttcagcccga ctgctgcgcc ttatccggta actgttcact tgagtccaac
      721 ccggaaaagc acggtaaaac gccactggca gcagccattg gtaactggga gttcgcagag
      781 gatttgttta gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc ggctacactg
      841 gaaggacaga tttggttgct gtgctctgcg aaagccagtt accacggtta agncagttss
      901 ssaactgnct taaccttcga tcaaaccacc tccccaggtg gttttttcgw ttacagggca
      961 aaagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctactgaacc
     1021 gctctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg
     1081 atataagttg taattctcat gttagtcatg ccccgcgccc accggaagga gctgactggg
     1141 ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca
     1201 ttaattgcgt tgcgctaata cgactcacta tagggagacc acaacggttt ccctccggct
     1261 ccggaaataa ttttgtttaa ctttaagaag gagatataca tatggggagt tcgcaccatc
     1321 atcaccacca tagctctggc tcctatccgt atgacgtgcc agactatgct ggtagctatc
     1381 cttacgatgt ccccgattat gcgggatctt atccatacga tgtgccagac tatgcaagtt
     1441 caggttccct ggaagttctg ttccaggggc ccggatcccg gtccgacata tgagcgcgcg
     1501 gaattcaaag gcctacgtcg acgagctcac tagtcgcggc cgctttcgaa tctagagcct
     1561 gcagtctcga caagcttgtc gagaagtact agaggatgta caacgcgtgc gcgctgatcc
     1621 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     1681 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aacctcaggc
     1741 atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata
     1801 gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcat cgtggccgga
     1861 tcttgcggcc cctcggcttg aacgaattgt tagacattat ttgccgacta ccttggtgat
     1921 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1981 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     2041 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     2101 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     2161 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     2221 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     2281 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     2341 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     2401 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     2461 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     2521 tacggtcacc gtaaccagca aatcaatatc actgtgtggc t
//
