LOCUS       sequence-319355-        2684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term Myc 3C cleavable Spec resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 33 pCOLI_S_Myc
FEATURES             Location/Qualifiers
     source          1..2684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(130..221)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(269..1008)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     primer_bind     1218..1237
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1218..1236
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1274..1296
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1316..1333
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1346..1375
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1382..1411
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1418..1447
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1454..1483
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1490..1519
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1526..1555
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1574..1597
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(1790..1808)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1804..1851
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(join(2022..2684,1..129))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag
       61 atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc
      121 ttccctcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      181 gagcggatac atatttgaat gtatttagaa aaataaacaa atagctagct cactcggtcg
      241 ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca catacaaagt tacccacaga
      301 ttccgtggat aagcagggga ctaacatgtg aggcaaaaca gcagggccgc gccggtggcg
      361 tttttccata ggctccgccc tcctgccaga gttcacataa acagacgctt ttccggtgca
      421 tctgtgggag ccgtgaggct caaccatgaa tctgacagta cgggcgaaac ccgacaggac
      481 ttaaagatcc ccaccgtttc cggcgggtcg ctccctcttg cgctctcctg ttccgaccct
      541 gccgtttacc ggatacctgt tccgcctttc tcccttacgg gaagtgtggc gctttctcat
      601 agctcacaca ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg ggctgtaagc
      661 aagaactccc cgttcagccc gactgctgcg ccttatccgg taactgttca cttgagtcca
      721 acccggaaaa gcacggtaaa acgccactgg cagcagccat tggtaactgg gagttcgcag
      781 aggatttgtt tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt ccggctacac
      841 tggaaggaca gatttggttg ctgtgctctg cgaaagccag ttaccacggt taagncagtt
      901 ssssaactgn cttaaccttc gatcaaacca cctccccagg tggttttttc gtttacaggg
      961 caaaagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctactgaa
     1021 ccgctctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata
     1081 cgatataagt tgtaattctc atgttagtca tgccccgcgc ccaccggaag gagctgactg
     1141 ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta
     1201 cattaattgc gttgcgctaa tacgactcac tatagggaga ccacaacggt ttccctccgg
     1261 ctccggaaat aattttgttt aactttaaga aggagatata catatgggaa gttctcatca
     1321 ccaccatcat catagttcgg gctccgagca gaaacttatc tctgaggaag acttaaatgg
     1381 ggagcagaag ttgatctcgg aggaagatct gaatggtgag caaaagttaa tctccgaaga
     1441 agacttgaat ggcgaacaaa aactgatctc agaggaagat ttgaacggcg aacagaagtt
     1501 aatctctgag gaggatctga acggagaaca gaaacttatt tcggaagaag atttgaacgg
     1561 gtcgagtggt agtctggaag ttctgttcca ggggcccgga tcccggtccg acatatgagc
     1621 gcgcggaatt caaaggccta cgtcgacgag ctcactagtc gcggccgctt tcgaatctag
     1681 agcctgcagt ctcgacaagc ttgtcgagaa gtactagagg atgtacaacg cgtgcgcgct
     1741 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     1801 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaacct
     1861 caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag
     1921 caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcatcgtgg
     1981 ccggatcttg cggcccctcg gcttgaacga attgttagac attatttgcc gactaccttg
     2041 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
     2101 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     2161 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     2221 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     2281 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     2341 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     2401 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     2461 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     2521 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
     2581 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
     2641 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtgg
//
