LOCUS       sequence-319358-        2316 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Untagged Spec resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 25 pCOLI_S_NoTag
FEATURES             Location/Qualifiers
     source          1..2316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     708..727
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        708..726
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             764..786
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(912..930)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      926..973
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1144..1935)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1936..2027)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(2075..2316,1..498))
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 ctccctcttg cgctctcctg ttccgaccct gccgtttacc ggatacctgt tccgcctttc
       61 tcccttacgg gaagtgtggc gctttctcat agctcacaca ctggtatctc ggctcggtgt
      121 aggtcgttcg ctccaagctg ggctgtaagc aagaactccc cgttcagccc gactgctgcg
      181 ccttatccgg taactgttca cttgagtcca acccggaaaa gcacggtaaa acgccactgg
      241 cagcagccat tggtaactgg gagttcgcag aggatttgtt tagctaaaca cgcggttgct
      301 cttgaagtgt gcgccaaagt ccggctacac tggaaggaca gatttggttg ctgtgctctg
      361 cgaaagccag ttaccacggt taagncagtt ssssaactgn cttaaccttc gatcaaacca
      421 cctccccagg tggttttttc gwwtacaggg caaaagatta cgcgcagaaa aaaaggatct
      481 caagaagatc ctttgatctt ttctactgaa ccgctctaga tttcagtgca atttatctct
      541 tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc atgttagtca
      601 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag
      661 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctaa tacgactcac
      721 tatagggaga ccacaacggt ttccctccgg ctccggaaat aattttgttt aactttaaga
      781 aggagactgg atccgaatct agagcctgca gtctcgacaa gcttgtcgag aagtactaga
      841 ggatgtacaa cgcgtgcgcg ctgatccggc tgctaacaaa gcccgaaagg aagctgagtt
      901 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
      961 gaggggtttt ttgctgaaac ctcaggcatt tgagaagcac acggtcacac tgcttccggt
     1021 agtcaataaa ccggtaaacc agcaatagac ataagcggct atttaacgac cctgccctga
     1081 accgacgacc gggtcatcgt ggccggatct tgcggcccct cggcttgaac gaattgttag
     1141 acattatttg ccgactacct tggtgatctc gcctttcacg tagtggacaa attcttccaa
     1201 ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc
     1261 aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc
     1321 cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac
     1381 atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag
     1441 cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac
     1501 caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt
     1561 ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc
     1621 gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac
     1681 agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa
     1741 agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact
     1801 gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg
     1861 ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat
     1921 caccgcttcc ctcatactct tcctttttca atattattga agcatttatc agggttattg
     1981 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag ctagctcact
     2041 cggtcgctac gctccgggcg tgagactgcg gcgggcgctg cggacacata caaagttacc
     2101 cacagattcc gtggataagc aggggactaa catgtgaggc aaaacagcag ggccgcgccg
     2161 gtggcgtttt tccataggct ccgccctcct gccagagttc acataaacag acgcttttcc
     2221 ggtgcatctg tgggagccgt gaggctcaac catgaatctg acagtacggg cgaaacccga
     2281 caggacttaa agatccccac cgtttccggc gggtcg
//
