LOCUS       sequence-319361-       11022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses TMC3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11022)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 11022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_TMC3_OE_1
FEATURES             Location/Qualifiers
     source          1..11022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        150..453
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        454..657
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     608..628
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    680..704
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4005..4029)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4031..4072
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4114..4702
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4167..4187)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4585..4596)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4774..5007
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5085..5206
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5122..5141)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5176..5195
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5246..5381
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5401..5420)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5402..5420)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5430..5447)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5430..5446)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5439..5461)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5588..6043
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5675..5694)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5885..5906
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6069..6173
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6174..6242,6243..7034)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6392..6411)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7205..7793
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7694..7713
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7947..7964
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8081..8102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8117..8122,8123..8140,8141..8147)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8155..8171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8160..8182
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8179..8195
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8179..8195
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8214..8234
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8216..8234
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8262..8488
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8489..8669
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8716..8841
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9334..9567
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9752..9796
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9971..10471
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     10390..10408
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             10483..10878
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    join(10938..11022,1..33)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
       61 ccactttggc gccggctcga gggggcccgg ggttattaat agtaatcaat tacggggtca
      121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      241 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      421 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      481 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      541 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      601 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
      661 tggctaactg tcgggatcaa caagtttgta caaaaaagca ggcaccatga aaacctcgaa
      721 ggcatcccag cgctatagag gcatccggag aaatgccagc cagtgctacc tctaccagga
      781 atctctgctg ctcagcaact tggatgacag ctttagtgct gatgaaacag gggacagcaa
      841 tgatccggaa caaatcttcc agaatataca gttccagaaa gatctcatgg caaacattcg
      901 ctgcagaccc tggactatgg gacagaagct gagggcattg agacaagcga agaacattgt
      961 gctgaagttt gaagggaggc tgaccaggac ccgaggctac caagcagcag gtgcagagct
     1021 ctggcggaaa tttgctcgtc tcgcctgtaa ctttgtggtc atcttcattc cctgggaaat
     1081 gaggataaag aaaatcgaga gtcattttgg atctggcgtt gcctcctatt tcatattctt
     1141 gagatggtta tttggaatta atattgtgct caccattatg acaggtgctt ttattgtcat
     1201 tccagagctg attgcaggcc agccctttgg aagcacagcc aggaagacca tccccaagga
     1261 gcaggtttcg tctgcccaag acctggacac cgtctggtct ctggggggct acctccagta
     1321 ctctgtcctc ttctacggat attacggcag ggagaggaag atcgggagag ctggctaccg
     1381 gctgcccttg gcgtatttcc tagtggggat ggcagtgttt gcttacagct tcatcattct
     1441 tttaaaaaag atggctaaga actcccgcac gagtcttgcc agtgcttcca atgaaaacta
     1501 taccttctgc tggcgggtgt tctgtgcctg ggattacctc attggaaacc cagaggctgc
     1561 agagagcaaa acagctgcca tagtgaacag catcagggaa gctatattgg aagaacagga
     1621 gaaaaagaaa agcaaaaacc tggcagtgac catctgcctg aggattattg ccaacatcct
     1681 ggtgcttctc tcactggctg ggagcattta tctcatctac tttgtggtgg accggtccca
     1741 gaagctggag cagtcgaaga aggagctgac actttgggaa aagaatgagg tcagtgtggt
     1801 ggtctccctc gtcaccatga tagcaccatc agcctttgac ctcattgctg ccttagagat
     1861 gtaccacccc aggaccacgc tgcgcttcca gcttgcaagg gtccttgtgc tgtatttggg
     1921 aaatctctac agcctgatca ttgctctcct ggacaaagtt aacagcatga gcattgagga
     1981 aatggctacc aaaaataaca caagtcattg gatagattcc accaccttct ttgcaaccag
     2041 gacagctcct gaagaagaga aatggtccac atctcggcct ggaatggggc tcaggagaaa
     2101 caacacatgg gccttggaag agaccagcat ttcagcttat accatgcctc ttataaaggc
     2161 caacaagact agcctccaca ctcagagtcc acaagaccag tgctgggaga catacgttgg
     2221 tcaggagatg ctgaagctct ccatcattga catgctcttc accgtggcga gcattctgct
     2281 catagacttc ttccgaggac ttttcgtgcg gtacttaagt gactactggt gttgggatct
     2341 ggaaagcaag tttcctgaat atggagaatt caaaatagct gagaatgtgc tacatttagt
     2401 ctacaaccaa ggaatgattt ggatgggggc cttcttctcc ccatgtctcc cagcgttcaa
     2461 cgttctcaaa ctcattgggc tcatgtacct gagaagctgg gctgtgctga cctgcaatgt
     2521 gccccaccag caagtatttc gagcctcaag atccaacaac ttctacttgg caatgctcct
     2581 gtttatgctg tttctgtgca tgctgccaac catttttgct attgtccgat acaagccatc
     2641 tctaaactgt gggccattca gtggacaaga gaaaatttat gacattgtgt cagaaacgat
     2701 tgagaaagac tttcctgtgt ggtttggctc cgtggttgga cacatcagta gccccgtggt
     2761 catcctgccc gcagtactcc tgcttttcat gctcatctat tatctccaga gcatcgcacg
     2821 gtctctaaag ctcagcaacc accagctcaa aatgcagatc caaaacgcaa gatcagagga
     2881 taagaaaaag gttgcccaga tggtagaagc ccgaatccag acccaggaag aaagcaccaa
     2941 gaagcttcca aacgacagtg atcttaccag ccagctgtcc tcagcgcact cgggcacacc
     3001 ccaaaacaac ggcaacgtgg cccattttga ctcagggagc agcaagagtg gcaggataga
     3061 gacagtcgca cagtccatgc cccagagccc gaggccaggg gacagggctc ctagctcacc
     3121 tctccctggg gtccccaaat ccaggctaga gcatgaaact aacaggtatc tgcacggtct
     3181 gtgtgcaagc accagtgacc ttcacaggaa cagatcgcgc acacctatga catttacaac
     3241 gcacatcgaa gatgtacact cagaacctct tttccgaaaa gactttcagc aaatcaaccc
     3301 tcctcaccgt ggaccacagg cctcgacttt gctggcacag ggtcccaggc cccacgcccc
     3361 cagatactat gtcattaatg aatatgactc ttacaagaaa aaacatctaa atgtctggcc
     3421 agaaagacat ttcaagatag atgcttcagg tgacattgtg gagctgtacc ccaggaacgt
     3481 gcgacaatat gcatcccggg tcccccgcca gcctccctcc ccacagctga gtgaagaaga
     3541 ggaggagacg ccaagcagag attggatcaa acggtctctt cccccacgct ccctgataga
     3601 cctccgtcgg gctcctcatt tttatattgg ggagaggtcc gaaagtcaga cccgggatcc
     3661 cgagcaccag gggagggtgc actacaagtc gtggaatgaa gattttgagg gtcaccttga
     3721 gaggccagcc tatgtgccca gaaagccacg atcccggaat ttccaatacc cacagccccc
     3781 tctgaagccc agagggaagc ccaggttcga gccatccctc acggaatctg actccgtgtc
     3841 ggcagcatcc agcagtgacc agcagaacag cagcgctgac cagtacctgc aggtcaccca
     3901 cagccagggc aggttcccga ggtccgtggg ccagcccagc aggaggaagg ccaagtcggg
     3961 gcaggagctg accgtggatc tggatgactt gatttgttca aatgtcacag ctttcttgta
     4021 caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg
     4081 agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     4141 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     4201 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     4261 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     4321 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     4381 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     4441 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4501 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     4561 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4621 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4681 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     4741 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     4801 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     4861 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     4921 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     4981 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     5041 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     5101 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5161 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     5221 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5281 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     5341 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     5401 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     5461 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     5521 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     5581 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5641 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5701 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5761 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5821 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5881 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     5941 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6001 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     6061 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6121 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6181 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6241 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6301 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6361 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     6421 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6481 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6541 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6601 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6661 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     6721 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6781 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6841 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6901 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6961 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7021 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7081 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7141 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7201 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7261 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7321 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7381 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7441 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7501 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7561 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7621 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7681 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7741 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7801 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7861 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7921 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     7981 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     8041 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     8101 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     8161 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     8221 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     8281 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     8341 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     8401 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     8461 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     8521 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8581 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8641 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     8701 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     8761 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     8821 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     8881 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     8941 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     9001 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     9061 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     9121 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     9181 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     9241 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     9301 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     9361 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     9421 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     9481 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     9541 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     9601 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     9661 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     9721 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     9781 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     9841 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     9901 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     9961 gaggggaccc ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
    10021 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
    10081 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
    10141 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
    10201 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
    10261 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
    10321 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
    10381 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
    10441 cgttgaccga atcaccgacc tctctcccca gggggtacca ccatggccaa gcctttgtct
    10501 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct
    10561 gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc
    10621 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct
    10681 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc
    10741 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc
    10801 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct
    10861 ggttatgtgt gggagggcct gcagctgcag tagtaagaat tctagatctt gagacaaatg
    10921 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
    10981 agaatagtag acataatagc aacagacata caaactaaag aa
//
