LOCUS       sequence-319364-        9309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses KCNA6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9309)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_KCNA6_OE_1
FEATURES             Location/Qualifiers
     source          1..9309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        33..336
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        337..540
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     491..511
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    563..587
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2175..2199)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2201..2242
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2284..2872
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2337..2357)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2755..2766)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2944..3177
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3255..3376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3292..3311)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3346..3365
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3416..3551
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3571..3590)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3572..3590)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3600..3617)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3600..3616)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3609..3631)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3758..4213
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3845..3864)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4055..4076
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4239..4343
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4344..4412,4413..5204)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4562..4581)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5375..5963
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5864..5883
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6117..6134
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6251..6272
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6287..6292,6293..6310,6311..6317)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6325..6341
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6330..6352
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6349..6365
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6349..6365
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6384..6404
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6386..6404
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6432..6658
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6659..6839
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6886..7011
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7504..7737
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7922..7966
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8141..8641
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8560..8578
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8653..9048
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9108..9225
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
       61 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      121 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      181 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      241 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      301 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      361 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      421 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      481 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      541 ctctggctaa ctgtcgggat caacaagttt gtacaaaaaa gttggcatga gatcggagaa
      601 atcccttacg ctggcggcgc cgggggaggt ccgtgggccg gagggagagc aacaggatgc
      661 gggagacttc ccggaggccg gcgggggcgg gggctgctgt agtagcgagc ggctggtgat
      721 caatatctcc gggctgcgct ttgagacaca attgcgcacc ctgtcgctgt ttccggacac
      781 gctgctcgga gaccctggcc ggcgagtccg cttcttcgac cccctgagga acgagtactt
      841 cttcgaccgc aaccggccca gcttcgacgc catcctctac tactaccagt ctgggggccg
      901 cctgcggagg ccggtcaacg tgcccctgga cattttcctg gaggagatcc gcttctacca
      961 gctgggggac gaggccctgg cggccttccg ggaggacgag ggctgcctgc ccgaaggtgg
     1021 cgaggacgag aagccgctgc cctcccagcc cttccagcgc caggtgtggc tgctctttga
     1081 gtacccagag agctctgggc cggccagggg catcgccatc gtctccgtgt tggtcattct
     1141 catctccata gtcatctttt gcctggagac cttaccccag ttccgtgtag atggtcgagg
     1201 tggaaacaat ggtggtgtga gtcgagtctc cccagtttcc agggggagtc aggaggaaga
     1261 ggaggatgaa gacgattcct acacatttca tcatggcatc acccctgggg aaatggggac
     1321 cgggggctcc tcctcactca gtactcttgg gggctccttc tttacagacc ccttctttct
     1381 ggtggagacg ctgtgcattg tctggttcac ttttgagctc ctggtgcgct tctccgcctg
     1441 ccctagcaag ccggccttct tccggaacat catgaacatc attgacttgg tggctatctt
     1501 cccctacttc atcaccctgg gcactgagct ggtgcagcag caggagcagc aaccagccag
     1561 tggaggaggc ggccagaatg ggcagcaggc catgtccctg gccatcctcc gagtcatccg
     1621 cctggtccgg gtgttccgca tcttcaagct ctcccgccac tccaaggggc tgcagatcct
     1681 gggcaagacc ttgcaggcct ccatgaggga gctggggctg ctcatcttct tcctcttcat
     1741 cggggtcatc ctcttctcca gtgccgtcta cttcgcagag gctgacgatg acgattcgct
     1801 ttttcccagc atcccggatg ccttctggtg ggcagtggtt acaatgacca cggtaggtta
     1861 cggggacatg taccccatga ctgtgggggg aaagatcgtg ggctcgctgt gtgccatcgc
     1921 tggggtcctc accattgccc tgcctgtgcc cgtcatcgtc tccaacttca actacttcta
     1981 ccaccgggag acggagcagg aggagcaagg ccagtatacc cacgtcactt gtgggcagcc
     2041 tgcgccggac ctgagggcaa ctgacaacgg acttggcaag cctgacttcc ccgaggctaa
     2101 ccgggaacgg agacccagct accttcctac accacatcgg gcctatgcag agaaaagaat
     2161 gctcacggag gtctgcccaa ctttcttgta caaagtggtt ggtaagccta tccctaaccc
     2221 tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc gcgttaagtc
     2281 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     2341 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     2401 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     2461 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     2521 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     2581 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     2641 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     2701 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     2761 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     2821 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     2881 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     2941 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     3001 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     3061 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     3121 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     3181 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     3241 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     3301 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3361 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     3421 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     3481 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     3541 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     3601 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     3661 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     3721 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     3781 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3841 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3901 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3961 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4021 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4081 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4141 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4201 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4261 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4321 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4381 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4441 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4501 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4561 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4621 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4681 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4741 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4801 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4861 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4921 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     4981 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5041 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5101 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5161 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5221 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5281 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5341 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5401 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5461 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5521 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5581 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5641 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5701 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5761 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5821 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5881 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5941 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6001 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6061 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     6121 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     6181 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     6241 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     6301 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     6361 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     6421 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     6481 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     6541 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     6601 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     6661 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6721 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6781 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6841 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     6901 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     6961 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     7021 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     7081 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     7141 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     7201 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     7261 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     7321 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     7381 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     7441 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     7501 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     7561 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     7621 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     7681 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     7741 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     7801 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     7861 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     7921 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     7981 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     8041 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     8101 attatcgttt cagacccacc tcccaacccc gaggggaccc ttgcgccttt tccaaggcag
     8161 ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc
     8221 cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc
     8281 tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt
     8341 gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc
     8401 agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag
     8461 cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg
     8521 gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc
     8581 tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca
     8641 gggggtacca ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca
     8701 acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc
     8761 tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt
     8821 gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc
     8881 gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg
     8941 cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg
     9001 gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcct gcagctgcag
     9061 tagtaagaat tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa
     9121 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     9181 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     9241 gacagcagag atccactttg gcgccggctc gagggggccc ggggttatta atagtaatca
     9301 attacgggg
//
