LOCUS       sequence-319365-        9141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses BEST4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9141)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_BEST4_OE_1
FEATURES             Location/Qualifiers
     source          1..9141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        58..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        362..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     516..536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    588..612
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2032..2056)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2058..2099
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2141..2729
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2194..2214)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2612..2623)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2801..3034
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3112..3233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3149..3168)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3203..3222
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3273..3408
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3428..3447)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3429..3447)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3457..3474)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3457..3473)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3466..3488)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3615..4070
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3702..3721)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3912..3933
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4096..4200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4201..4269,4270..5061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4419..4438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5232..5820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5721..5740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5974..5991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6108..6129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6144..6149,6150..6167,6168..6174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6182..6198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6187..6209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6206..6222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6206..6222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6241..6261
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6243..6261
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6289..6515
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6516..6696
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6743..6868
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7361..7594
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7779..7823
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7998..8498
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8417..8435
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8510..8905
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8965..9082
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctctctg gctaactgtc gggatcaaca agtttgtaca
      601 aaaaagcagg caccatgacg gtttcataca ctctcaaagt ggcggaggcc cgcttcggag
      661 gtttctctgg cctgcttctc cgctggaggg gaagcatcta caagctcctc tacaaggaat
      721 tcctcctctt tggggccttg tacgctgtgc ttagcatcac ctaccggctg ctgctgaccc
      781 aggagcagag gtacgtgtat gctcaggtgg cccggtactg caaccgctca gcagacctca
      841 ttcccttgtc ctttgtattg ggtttctatg tgactctcgt ggtgaaccgc tggtggtccc
      901 agtacacaag catcccgctg ccagaccagc tgatgtgcgt catctcggct agcgtgcacg
      961 gcgtggacca gcggggccgc ctgctgcgcc gcaccctcat ccgctacgcg aacctggcgt
     1021 ccgtgctggt gctgcgctcg gtcagcaccc gcgtgcttaa gcgcttcccc accatggagc
     1081 acgtggtgga tgcaggtttc atgtcccagg aagagaggaa aaagtttgag agcctgaaat
     1141 ccgacttcaa caagtactgg gtcccctgcg tctggttcac caacctggcg gcccaggccc
     1201 ggagggacgg gcgaatacgt gacgatatcg ctctctgtct acttttggaa gagctgaaca
     1261 agtaccgagc caagtgcagc atgctattcc actatgactg gatcagcatc cccctcgtct
     1321 acacccaagt ggtgaccata gccgtctact ctttctttgc cctctccctg gttggccgcc
     1381 agtttgtgga gccagaggca ggggctgcca aacctcagaa gcttctgaag ccaggccagg
     1441 agccagcccc agccctggga gacccggaca tgtacgtgcc tctcaccact ctgctgcagt
     1501 tcttcttcta tgctggctgg ctcaaggtgg ctgaacagat catcaaccca tttggtgagg
     1561 atgatgacga ctttgagaca aatcagctca tagaccgcaa cttgcaggtg tccctgctat
     1621 ccgtggacga aatgtaccag aaccttcccc ccgctgagaa ggaccagtac tgggatgagg
     1681 accagccgca gccaccctac actgtggcca cggcggccga gtctctgcgg ccctcattcc
     1741 tgggctccac cttcaacctg cgcatgagcg acgaccctga gcagagcctg caggtggagg
     1801 cgtcccccgg atctggtcgg cccgcgcccg ccgcgcagac cccgttgctc ggccgcttcc
     1861 tgggcgtagg ggcgccctcc ccggccatca gcctccggaa cttcggccgc gtgcgaggca
     1921 ccccccgccc cccgcatctg ctgcgcttcc gggcggagga gggcggcgac cccgaggccg
     1981 cagcccgcat cgaggaggaa tcggcggagt ccggggacga ggccctggag cccacagctt
     2041 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
     2101 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggattacaa
     2161 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     2221 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     2281 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     2341 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     2401 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     2461 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     2521 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     2581 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     2641 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     2701 tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca
     2761 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     2821 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     2881 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     2941 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     3001 agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt
     3061 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     3121 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3181 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     3241 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3301 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3361 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     3421 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     3481 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     3541 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     3601 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3661 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     3721 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     3781 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     3841 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     3901 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     3961 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4021 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     4081 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4141 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4201 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4261 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4321 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4381 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4441 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4501 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4561 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4621 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4681 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4741 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4801 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4861 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4921 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4981 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5041 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5101 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5161 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5221 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5281 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5341 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5401 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5461 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5521 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5581 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5641 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5701 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5761 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5821 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5881 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5941 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6001 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     6061 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     6121 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     6181 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     6241 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     6301 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     6361 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     6421 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     6481 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     6541 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6601 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6661 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     6721 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     6781 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     6841 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     6901 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     6961 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     7021 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     7081 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     7141 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     7201 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     7261 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     7321 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     7381 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     7441 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     7501 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     7561 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     7621 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     7681 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     7741 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     7801 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     7861 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     7921 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     7981 caaccccgag gggacccttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg
     8041 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct
     8101 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga
     8161 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt
     8221 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa
     8281 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg
     8341 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc
     8401 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg
     8461 gctccctcgt tgaccgaatc accgacctct ctccccaggg ggtaccacca tggccaagcc
     8521 tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc
     8581 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac
     8641 tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac
     8701 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag
     8761 gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat
     8821 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct
     8881 gccctctggt tatgtgtggg agggcctgca gctgcagtag taagaattct agatcttgag
     8941 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     9001 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     9061 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     9121 ccggctcgag ggggcccggg g
//
