LOCUS       sequence-319373-        9413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generation of retrovirus for the overexpression of Nr4a1 mutant 
            (287C 288EAA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Zhang Y, Federation AJ, Kim S, O'Keefe JP, Lun M, Xiang D, Brown JD,
            Steinhauser ML
  TITLE     Targeting nuclear receptor NR4A1-dependent adipocyte progenitor 
            quiescence promotes metabolic adaptation to obesity.
  JOURNAL   J Clin Invest. 2018 Nov 1;128(11):4898-4911. doi: 10.1172/JCI98353. 
            Epub 2018 Oct 2.
  PUBMED    30277475
REFERENCE   2  (bases 1 to 9413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1172/JCI98353"; 
            journalName: "J Clin Invest"; date: "2018-11-1- 1"; volume: "128"; 
            issue: "11"; pages: "4898-4911"
COMMENT     Sequence Label: MSCV-HA-Nr4a1-AA
FEATURES             Location/Qualifiers
     source          1..9413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1431..1457
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3306..3329)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3656..3675
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3744..4343
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3744..3763)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4240..4260
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4404..4971
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4421..4973
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4565..4582)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4792..4811
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4985..4987,4988..4990,4991..5704)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5030..5051)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5291..5310)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5638..5659
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5806..6320
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6482..6498
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6506..6512,6513..6530,6531..6536))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6551..6572
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6689..6706)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6860..7448)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6940..6959)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7619..8410,8411..8479))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8242..8261
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8480..8584)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8652..8670
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8708..8730)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8830..8849
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9043..9065
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9193..9212
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9412..9413,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctctcga ggccaccatg tacccatacg
     1441 atgttccgga ttacgctccc tgtattcaag ctcaatatgg aacaccagca acgagcccag
     1501 gaccgcgtga ccacctgacc ggtgatcccc tggcccttga gttcggcaag cctaccatgg
     1561 acctggccag ccccgagaca gcacctgccg cacctgctac actgcccagc ttcagcacct
     1621 tcatggacgg gtacaccgga gagtttgaca ccttcctcta ccagctgccg gggacgaccc
     1681 agccgtgctc ctcagcttgt tcctctgcct cctccacgtc ttcttcctca tcctcggcca
     1741 cctcccccgc ttcggcgtcc ttcaagtttg aggacttcca ggtgtacggc tgctacccgg
     1801 gcaccctgag cggcccatta gatgagaccc tatcctccag cggctctgag tactatggca
     1861 gtccctgctc agccccctcg ccatctacac ccaacttcca gccgtcccag ctttctccct
     1921 gggacggctc atttggccac ttctccccga gccagactta tgaaggcctc tgggcatgga
     1981 cagagcagtt gcctaaggct tcttcagggc ctccgccacc tccaaccttc ttctccttca
     2041 gtcctcccac tggccccagc cccagcctgg cccagagttc tctgaaattg ttcccaccac
     2101 cagccaccca ccagcttggg gagggggaga gctattccat gccagcagct ttccccggct
     2161 tggcacccac ctctccgaac cgtgacactt ccggcattct ggacgcaccc gtgacctcca
     2221 ccaagtcccg gagcggggct tcaggtggca gcgagggccg ctgtgcagtc tgtggtgaca
     2281 atgcttcgtg tcagcactat ggggtccgca ccgcagctgg ctgcaagggc ttcttcaagc
     2341 gcacagtaca gaaaagcgcc aagtacatct gcctggcaaa caaggattgc cctgtggaca
     2401 agaggcggcg gaaccgctgc cagttctgcc gcttccagaa gtgcctggct gtgggcatgg
     2461 tgaaggaagt tgtacggaca gacagcctaa aagggcggcg gggccggcta ccttcaaaac
     2521 ccaagcagcc tccagatgcc tcccctacca atcttctcac ttccctcatc cgggcacact
     2581 tggactccgg gcctagcact gccaaattgg actattccaa gttccaggaa ctggtgctgc
     2641 cccgcttcgg gaaggaagat gccggtgacg tgcaacaatt ttatgacttg ctctctggtt
     2701 ccctggacgt tatccgaaag tgggcagaaa aaatccctgg cttcattgag ctttgcccag
     2761 gagaccaaga cctgttgcta gagtctgcct tcctggaact cttcatcctc cgcctggcat
     2821 accgatctaa acccggtgag gggaagctca tcttctgctc aggcctggta ctacaccagc
     2881 tgcagtgtgc ccgtggcttt ggtgattgga ttgacaacat cctggccttc tcacggtccc
     2941 tgcacagctt gggtgttgat gttcccgcct ttgcctgcct gtccgctctg gtcctcatca
     3001 ctgatcgaca cgggctccag gaccctcgtc gggtggaaga gctgcagaat cgcattgcta
     3061 gctgtctgaa ggagcacatg gctaccgtgg caggagaccc acagccggcc agctgcctgt
     3121 cacgtctgct gggcaaactg cctgagcttc ggaccctgtg cactcaaggc ctgcagcgca
     3181 tcttttgcct caagttggag gacttggtac cccctccacc tattgtggac aagatcttta
     3241 tggacacatt gtctttctga gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     3301 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     3361 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     3421 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     3481 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     3541 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     3601 tcgctttctg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     3661 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     3721 tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     3781 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     3841 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     3901 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     3961 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     4021 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     4081 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     4141 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     4201 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     4261 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     4321 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
     4381 gcgcacgacc ccatgcatcc aattccgccc ccccccccta acgttactgg ccgaagccgc
     4441 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     4501 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     4561 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     4621 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     4681 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     4741 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     4801 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     4861 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     4921 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac
     4981 aaccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     5041 ggacggcgac gtaaacggcc acaagttcag cgtgtctggc gagggcgagg gcgatgccac
     5101 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     5161 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     5221 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     5281 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     5341 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     5401 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     5461 gaacggcatc aaggcgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     5521 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     5581 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     5641 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     5701 gtaatgaatt aattaagaat tatcaagctt atcgataccg tcgacctgca gccaagctta
     5761 tcgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
     5821 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aatacataac
     5881 tgagaataga gaagttcaga tcaaggttag gaacagagag acagcagaat atgggccaaa
     5941 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
     6001 gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
     6061 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
     6121 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggcgcgc
     6181 cagtcctccg atagactgcg tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt
     6241 tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc
     6301 cgtcagcggg ggtctttcat gggtaacagt ttcttgaagt tggagaacaa cattctgagg
     6361 gtaggagtcg aatattaagt aatcctgact caattagcca ctgttttgaa tccacatact
     6421 ccaatactcc tgaaatagtt cattatggac agcgcagaaa gagctgggga gaattgtgaa
     6481 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6541 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6601 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6661 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6721 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6781 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6841 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6901 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6961 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7021 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7081 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7141 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7201 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7261 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7321 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7381 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7441 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7501 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7561 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7621 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7681 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7741 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7801 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7861 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7921 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7981 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8041 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8101 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8161 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8221 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8281 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8341 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8401 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8461 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8521 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8581 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     8641 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     8701 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     8761 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     8821 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     8881 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
     8941 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     9001 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     9061 aaacgacggc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac
     9121 ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg
     9181 atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt
     9241 gatgccggcc acgatgcgtc cggcgtagag gcgattagtc caatttgtta aagacaggat
     9301 atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta
     9361 cgagccatag ataaaataaa agattttatt tagtctccag aaaaaggggg gaa
//
