LOCUS       sequence-319384-        9349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generation of retrovirus for the overexpression of Nr4a1 mutant 
            (zinc finger 2 deletion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9349)
  AUTHORS   Zhang Y, Federation AJ, Kim S, O'Keefe JP, Lun M, Xiang D, Brown JD,
            Steinhauser ML
  TITLE     Targeting nuclear receptor NR4A1-dependent adipocyte progenitor 
            quiescence promotes metabolic adaptation to obesity.
  JOURNAL   J Clin Invest. 2018 Nov 1;128(11):4898-4911. doi: 10.1172/JCI98353. 
            Epub 2018 Oct 2.
  PUBMED    30277475
REFERENCE   2  (bases 1 to 9349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1172/JCI98353"; 
            journalName: "J Clin Invest"; date: "2018-11-1- 1"; volume: "128"; 
            issue: "11"; pages: "4898-4911"
COMMENT     Sequence Label: MSCV-HA-Nr4a1-Zf2
FEATURES             Location/Qualifiers
     source          1..9349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1431..1457
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3231..3254)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3592..3611
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3680..4279
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3680..3699)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4176..4196
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4340..4907
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4357..4909
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4501..4518)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4728..4747
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4921..4923,4924..4926,4927..5640)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4966..4987)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5227..5246)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5574..5595
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5742..6256
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6418..6434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6442..6448,6449..6466,6467..6472))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6487..6508
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6625..6642)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6796..7384)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6876..6895)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7555..8346,8347..8415))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8178..8197
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8416..8520)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8588..8606
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8644..8666)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8766..8785
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8979..9001
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9129..9148
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9348..9349,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaacctaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctctcga ggccaccatg tacccatacg
     1441 atgttccgga ttacgctccc tgtattcaag ctcaatatgg aacaccagca acgagcccag
     1501 gaccgcgtga ccacctgacc ggtgatcccc tggcccttga gttcggcaag cctaccatgg
     1561 acctggccag ccccgagaca gcacctgccg cacctgctac actgcccagc ttcagcacct
     1621 tcatggacgg gtacaccgga gagtttgaca ccttcctcta ccagctgccg gggacgaccc
     1681 agccgtgctc ctcagcttgt tcctctgcct cctccacgtc ttcttcctca tcctcggcca
     1741 cctcccccgc ttcggcgtcc ttcaagtttg aggacttcca ggtgtacggc tgctacccgg
     1801 gcaccctgag cggcccatta gatgagaccc tatcctccag cggctctgag tactatggca
     1861 gtccctgctc agccccctcg ccatctacac ccaacttcca gccgtcccag ctttctccct
     1921 gggacggctc atttggccac ttctccccga gccagactta tgaaggcctc tgggcatgga
     1981 cagagcagtt gcctaaggct tcttcagggc ctccgccacc tccaaccttc ttctccttca
     2041 gtcctcccac tggccccagc cccagcctgg cccagagttc tctgaaattg ttcccaccac
     2101 cagccaccca ccagcttggg gagggggaga gctattccat gccagcagct ttccccggct
     2161 tggcacccac ctctccgaac cgtgacactt ccggcattct ggacgcaccc gtgacctcca
     2221 ccaagtcccg gagcggggct tcaggtggca gcgagggccg ctgtgcagtc tgtggtgaca
     2281 atgcttcgtg tcagcactat ggggtccgca cctgtgaggg ctgcaagggc ttcttcaagc
     2341 gcacagtaca gaaaagcgcc aagtacatcc tggctgtggg catggtgaag gaagttgtac
     2401 ggacagacag cctaaaaggg cggcggggcc ggctaccttc aaaacccaag cagcctccag
     2461 atgcctcccc taccaatctt ctcacttccc tcatccgggc acacttggac tccgggccta
     2521 gcactgccaa attggactat tccaagttcc aggaactggt gctgccccgc ttcgggaagg
     2581 aagatgccgg tgacgtgcaa caattttatg acttgctctc tggttccctg gacgttatcc
     2641 gaaagtgggc agaaaaaatc cctggcttca ttgagctttg cccaggagac caagacctgt
     2701 tgctagagtc tgccttcctg gaactcttca tcctccgcct ggcataccga tctaaacccg
     2761 gtgaggggaa gctcatcttc tgctcaggcc tggtactaca ccagctgcag tgtgcccgtg
     2821 gctttggtga ttggattgac aacatcctgg ccttctcacg gtccctgcac agcttgggtg
     2881 ttgatgttcc cgcctttgcc tgcctgtccg ctctggtcct catcactgat cgacacgggc
     2941 tccaggaccc tcgtcgggtg gaagagctgc agaatcgcat tgctagctgt ctgaaggagc
     3001 acatggctac cgtggcagga gacccacagc cggccagctg cctgtcacgt ctgctgggca
     3061 aactgcctga gcttcggacc ctgtgcactc aaggcctgca gcgcatcttt tgcctcaagt
     3121 tggaggactt ggtaccccct ccacctattg tggacaagat ctttatggac acattgtctt
     3181 tctgagaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     3241 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     3301 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     3361 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     3421 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     3481 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     3541 agaggctggg aaggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     3601 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     3661 cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     3721 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     3781 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     3841 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     3901 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     3961 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     4021 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     4081 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     4141 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     4201 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     4261 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     4321 acgaccccat gcatccaatt ccgccccccc cccctaacgt tactggccga agccgcttgg
     4381 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     4441 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     4501 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     4561 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     4621 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     4681 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     4741 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     4801 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     4861 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc
     4921 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4981 ggcgacgtaa acggccacaa gttcagcgtg tctggcgagg gcgagggcga tgccacctac
     5041 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     5101 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     5161 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     5221 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     5281 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     5341 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     5401 ggcatcaagg cgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     5461 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     5521 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     5581 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     5641 tgaattaatt aagaattatc aagcttatcg ataccgtcga cctgcagcca agcttatcga
     5701 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     5761 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaata cataactgag
     5821 aatagagaag ttcagatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg
     5881 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
     5941 cggtcccgcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
     6001 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     6061 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt
     6121 cctccgatag actgcgtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
     6181 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
     6241 agcgggggtc tttcatgggt aacagtttct tgaagttgga gaacaacatt ctgagggtag
     6301 gagtcgaata ttaagtaatc ctgactcaat tagccactgt tttgaatcca catactccaa
     6361 tactcctgaa atagttcatt atggacagcg cagaaagagc tggggagaat tgtgaaattg
     6421 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6481 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6541 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6601 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6661 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6721 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6781 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6841 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6901 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6961 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7021 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7081 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7141 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7201 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7261 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7321 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7381 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7441 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7501 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7561 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7621 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7681 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7741 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7801 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7861 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7921 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7981 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8041 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8101 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8161 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8221 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8281 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8341 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8401 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8461 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8521 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     8581 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     8641 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     8701 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     8761 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     8821 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     8881 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     8941 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     9001 gacggcgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg
     9061 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
     9121 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
     9181 ccggccacga tgcgtccggc gtagaggcga ttagtccaat ttgttaaaga caggatatca
     9241 gtggtccagg ctctagtttt gactcaacaa tatcaccagc tgaagcctat agagtacgag
     9301 ccatagataa aataaaagat tttatttagt ctccagaaaa aggggggaa
//
