LOCUS       sequence-319395-        9460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generation of retrovirus for the overexpression of Nr4a1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9460)
  AUTHORS   Zhang Y, Federation AJ, Kim S, O'Keefe JP, Lun M, Xiang D, Brown JD,
            Steinhauser ML
  TITLE     Targeting nuclear receptor NR4A1-dependent adipocyte progenitor 
            quiescence promotes metabolic adaptation to obesity.
  JOURNAL   J Clin Invest. 2018 Nov 1;128(11):4898-4911. doi: 10.1172/JCI98353. 
            Epub 2018 Oct 2.
  PUBMED    30277475
REFERENCE   2  (bases 1 to 9460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1172/JCI98353"; 
            journalName: "J Clin Invest"; date: "2018-11-1- 1"; volume: "128"; 
            issue: "11"; pages: "4898-4911"
COMMENT     Sequence Label: MSCV-HA-Nr4a1
FEATURES             Location/Qualifiers
     source          1..9460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1431..1457
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        3271..3770
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3306..3329)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3703..3722
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3791..4390
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3791..3810)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4287..4307
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4451..5018
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4468..5020
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4612..4629)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4839..4858
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5032..5034,5035..5037,5038..5751)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5077..5098)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5338..5357)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5685..5706
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5853..6367
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6529..6545
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6553..6559,6560..6577,6578..6583))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6598..6619
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6736..6753)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6907..7495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6987..7006)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7666..8457,8458..8526))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8289..8308
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8527..8631)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8699..8717
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8755..8777)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8877..8896
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9090..9112
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9240..9259
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9459..9460,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctctcga ggccaccatg tacccatacg
     1441 atgttccgga ttacgctccc tgtattcaag ctcaatatgg aacaccagca acgagcccag
     1501 gaccgcgtga ccacctgacc ggtgatcccc tggcccttga gttcggcaag cctaccatgg
     1561 acctggccag ccccgagaca gcacctgccg cacctgctac actgcccagc ttcagcacct
     1621 tcatggacgg gtacaccgga gagtttgaca ccttcctcta ccagctgccg gggacgaccc
     1681 agccgtgctc ctcagcttgt tcctctgcct cctccacgtc ttcttcctca tcctcggcca
     1741 cctcccccgc ttcggcgtcc ttcaagtttg aggacttcca ggtgtacggc tgctacccgg
     1801 gcaccctgag cggcccatta gatgagaccc tatcctccag cggctctgag tactatggca
     1861 gtccctgctc agccccctcg ccatctacac ccaacttcca gccgtcccag ctttctccct
     1921 gggacggctc atttggccac ttctccccga gccagactta tgaaggcctc tgggcatgga
     1981 cagagcagtt gcctaaggct tcttcagggc ctccgccacc tccaaccttc ttctccttca
     2041 gtcctcccac tggccccagc cccagcctgg cccagagttc tctgaaattg ttcccaccac
     2101 cagccaccca ccagcttggg gagggggaga gctattccat gccagcagct ttccccggct
     2161 tggcacccac ctctccgaac cgtgacactt ccggcattct ggacgcaccc gtgacctcca
     2221 ccaagtcccg gagcggggct tcaggtggca gcgagggccg ctgtgcagtc tgtggtgaca
     2281 atgcttcgtg tcagcactat ggggtccgca cctgtgaggg ctgcaagggc ttcttcaagc
     2341 gcacagtaca gaaaagcgcc aagtacatct gcctggcaaa caaggattgc cctgtggaca
     2401 agaggcggcg gaaccgctgc cagttctgcc gcttccagaa gtgcctggct gtgggcatgg
     2461 tgaaggaagt tgtacggaca gacagcctaa aagggcggcg gggccggcta ccttcaaaac
     2521 ccaagcagcc tccagatgcc tcccctacca atcttctcac ttccctcatc cgggcacact
     2581 tggactccgg gcctagcact gccaaattgg actattccaa gttccaggaa ctggtgctgc
     2641 cccgcttcgg gaaggaagat gccggtgacg tgcaacaatt ttatgacttg ctctctggtt
     2701 ccctggacgt tatccgaaag tgggcagaaa aaatccctgg cttcattgag ctttgcccag
     2761 gagaccaaga cctgttgcta gagtctgcct tcctggaact cttcatcctc cgcctggcat
     2821 accgatctaa acccggtgag gggaagctca tcttctgctc aggcctggta ctacaccagc
     2881 tgcagtgtgc ccgtggcttt ggtgattgga ttgacaacat cctggccttc tcacggtccc
     2941 tgcacagctt gggtgttgat gttcccgcct ttgcctgcct gtccgctctg gtcctcatca
     3001 ctgatcgaca cgggctccag gaccctcgtc gggtggaaga gctgcagaat cgcattgcta
     3061 gctgtctgaa ggagcacatg gctaccgtgg caggagaccc acagccggcc agctgcctgt
     3121 cacgtctgct gggcaaactg cctgagcttc ggaccctgtg cactcaaggc ctgcagcgca
     3181 tcttttgcct caagttggag gacttggtac cccctccacc tattgtggac aagatcttta
     3241 tggacacatt gtctttctga gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     3301 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     3361 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     3421 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     3481 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     3541 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     3601 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     3661 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     3721 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     3781 caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     3841 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     3901 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     3961 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     4021 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     4081 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     4141 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     4201 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     4261 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     4321 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     4381 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
     4441 tgcatccaat tccgcccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     4501 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     4561 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     4621 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     4681 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     4741 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     4801 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     4861 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     4921 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     4981 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc
     5041 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     5101 aacggccaca agttcagcgt gtctggcgag ggcgagggcg atgccaccta cggcaagctg
     5161 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     5221 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     5281 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     5341 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     5401 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     5461 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     5521 gcgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     5581 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     5641 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     5701 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta atgaattaat
     5761 taagaattat caagcttatc gataccgtcg acctgcagcc aagcttatcg ataaaataaa
     5821 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     5881 agctagctta agtaacgcca ttttgcaagg catggaaaat acataactga gaatagagaa
     5941 gttcagatca aggttaggaa cagagagaca gcagaatatg ggccaaacag gatatctgtg
     6001 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtcccgc
     6061 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
     6121 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
     6181 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgata
     6241 gactgcgtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg
     6301 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
     6361 ctttcatggg taacagtttc ttgaagttgg agaacaacat tctgagggta ggagtcgaat
     6421 attaagtaat cctgactcaa ttagccactg ttttgaatcc acatactcca atactcctga
     6481 aatagttcat tatggacagc gcagaaagag ctggggagaa ttgtgaaatt gttatccgct
     6541 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6601 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6661 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6721 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6781 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6841 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6901 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6961 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7021 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7081 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7141 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7201 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7261 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7321 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7381 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7441 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7501 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7561 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7621 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7681 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7741 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7801 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7861 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7921 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7981 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8041 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8101 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8161 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8221 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8281 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8341 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8401 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8461 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8521 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8581 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8641 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     8701 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     8761 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     8821 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     8881 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     8941 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     9001 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     9061 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggcgca
     9121 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     9181 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     9241 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     9301 atgcgtccgg cgtagaggcg attagtccaa tttgttaaag acaggatatc agtggtccag
     9361 gctctagttt tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagata
     9421 aaataaaaga ttttatttag tctccagaaa aaggggggaa
//
