LOCUS       sequence-319425-        9573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human LDLR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Frank M, Citarella CG, Quinones GB, Bentley M
  TITLE     A Novel Labeling Strategy Reveals That Myosin Va and Myosin Vb Bind 
            the Same Dendritically Polarized Vesicle Population.
  JOURNAL   Traffic. 2020 Sep 21. doi: 10.1111/tra.12764.
  PUBMED    32959500
REFERENCE   2  (bases 1 to 9573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2020 Sep 21. doi: 10.1111/tra.12764."
COMMENT     Sequence Label: pBa.Halo-LDLR
FEATURES             Location/Qualifiers
     source          1..9573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..276
                     /label=chicken beta-actin promoter
     primer_bind     1219..1241
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      1296..1305
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1401..1421
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             1437..2321
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             complement(4693..4704)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4992..5126
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5029..5048)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5083..5102
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5157..5173)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5157..5173)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5170..5192)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5181..5197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5205..5211,5212..5229,5230..5235))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5250..5271
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5388..5405)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5559..6147)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5639..5658)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6318..7109,7110..7178))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6941..6960
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7179..7283)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7351..7369
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7407..7429)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7529..7548
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7742..7764
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7756..7773
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7757..7773
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggsgggg gggggggggg
      121 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      181 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      241 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      301 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      361 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      421 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
      481 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
      541 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
      601 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
      661 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
      721 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
      781 acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg
      841 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
      901 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
      961 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     1021 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     1081 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     1141 gccgtcccct tctccatctc cagcctcggg gctgtccgca gggggacggc tgccttcggg
     1201 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggggtt tatatcttcc
     1261 cttctctgtt cctccgcagc ccccaagctt ggcgcgccac catggggcct tggggttgga
     1321 aattacgctg gactgttgcc ttgcttcttg ctgctgctgg aacagcagtt ggtgatagac
     1381 ctagcctcga gccaaccact gaggatctgt actttcagag cgataacgat ggatccgaaa
     1441 tcggtactgg ctttccattc gacccccatt atgtggaagt cctgggcgag cgcatgcact
     1501 acgtcgatgt tggtccgcgc gatggcaccc ctgtgctgtt cctgcacggt aacccgacct
     1561 cctcctacgt gtggcgcaac atcatcccgc atgttgcacc gacccatcgc tgcattgctc
     1621 cagacctgat cggtatgggc aaatccgaca aaccagacct gggttatttc ttcgacgacc
     1681 acgtccgctt catggatgcc ttcatcgaag ccctgggtct ggaagaggtc gtcctggtca
     1741 ttcacgactg gggctccgct ctgggtttcc actgggccaa gcgcaatcca gagcgcgtca
     1801 aaggtattgc atttatggag ttcatccgcc ctatcccgac ctgggacgaa tggccagaat
     1861 ttgcccgcga gaccttccag gccttccgca ccaccgacgt cggccgcaag ctgatcatcg
     1921 atcagaacgt ttttatcgag ggtacgctgc cgatgggtgt cgtccgcccg ctgactgaag
     1981 tcgagatgga ccattaccgc gagccgttcc tgaatcctgt tgaccgcgag ccactgtggc
     2041 gcttcccaaa cgagctgcca atcgccggtg agccagcgaa catcgtcgcg ctggtcgaag
     2101 aatacatgga ctggctgcac cagtcccctg tcccgaagct gctgttctgg ggcaccccag
     2161 gcgttctgat cccaccggcc gaagccgctc gcctggccaa aagcctgcct aactgcaagg
     2221 ctgtggacat cggcccgggt ctgaatctgc tgcaagaaga caacccggac ctgatcggca
     2281 gcgagatcgc gcgctggctg tctactctgg agatttccgg tctgtacaag acaggagggg
     2341 gagaattcga cagatgtgaa agaaacgagt tccagtgcca agacgggaaa tgcatctcct
     2401 acaagtgggt ctgcgatggc agcgctgagt gccaggatgg ctctgatgag tcccaggaga
     2461 cgtgcttgtc tgtcacctgc aaatccgggg acttcagctg tgggggccgt gtcaaccgct
     2521 gcattcctca gttctggagg tgcgatggcc aagtggactg cgacaacggc tcagacgagc
     2581 aaggctgtcc ccccaagacg tgctcccagg acgagtttcg ctgccacgat gggaagtgca
     2641 tctctcggca gttcgtctgt gactcagacc gggactgctt ggacggctca gacgaggcct
     2701 cctgcccggt gctcacctgt ggtcccgcca gcttccagtg caacagctcc acctgcatcc
     2761 cccagctgtg ggcctgcgac aacgaccccg actgcgaaga tggctcggat gagtggccgc
     2821 agcgctgtag gggtctttac gtgttccaag gggacagtag cccctgctcg gccttcgagt
     2881 tccactgcct aagtggcgag tgcatccact ccagctggcg ctgtgatggt ggccccgact
     2941 gcaaggacaa atctgacgag gaaaactgcg ctgtggccac ctgtcgccct gacgaattcc
     3001 agtgctctga tggaaactgc atccatggca gccggcagtg tgaccgggaa tatgactgca
     3061 aggacatgag cgatgaagtt ggctgcgtta atgtgacact ctgcgaggga cccaacaagt
     3121 tcaagtgtca cagcggcgaa tgcatcaccc tggacaaagt ctgcaacatg gctagagact
     3181 gccgggactg gtcagatgaa cccatcaaag agtgcgggac caacgaatgc ttggacaaca
     3241 acggcggctg ttcccacgtc tgcaatgacc ttaagatcgg ctacgagtgc ctgtgccccg
     3301 acggcttcca gctggtggcc cagcgaagat gcgaagatat cgatgagtgt caggatcccg
     3361 acacctgcag ccagctctgc gtgaacctgg agggtggcta caagtgccag tgtgaggaag
     3421 gcttccagct ggacccccac acgaaggcct gcaaggctgt gggctccatc gcctacctct
     3481 tcttcaccaa ccggcacgag gtcaggaaga tgacgctgga ccggagcgag tacaccagcc
     3541 tcatccccaa cctgaggaac gtggtcgctc tggacacgga ggtggccagc aatagaatct
     3601 actggtctga cctgtcccag agaatgatct gcagcaccca gcttgacaga gcccacggcg
     3661 tctcttccta tgacaccgtc atcagcaggg acatccaggc ccccgacggg ctggctgtgg
     3721 actggatcca cagcaacatc tactggaccg actctgtcct gggcactgtc tctgttgcgg
     3781 ataccaaggg cgtgaagagg aaaacgttat tcagggagaa cggctccaag ccaagggcca
     3841 tcgtggtgga tcctgttcat ggcttcatgt actggactga ctggggaact cccgccaaga
     3901 tcaagaaagg gggcctgaat ggtgtggaca tctactcgct ggtgactgaa aacattcagt
     3961 ggcccaatgg catcacccta gatctcctca gtggccgcct ctactgggtt gactccaaac
     4021 ttcactccat ctcaagcatc gatgtcaatg ggggcaaccg gaagaccatc ttggaggatg
     4081 aaaagaggct ggcccacccc ttctccttgg ccgtctttga ggacaaagta ttttggacag
     4141 atatcatcaa cgaagccatt ttcagtgcca accgcctcac aggttccgat gtcaacttgt
     4201 tggctgaaaa cctactgtcc ccagaggata tggtcctctt ccacaacctc acccagccaa
     4261 gaggagtgaa ctggtgtgag aggaccaccc tgagcaatgg cggctgccag tatctgtgcc
     4321 tccctgcccc gcagatcaac ccccactcgc ccaagtttac ctgcgcctgc ccggacggca
     4381 tgctgctggc cagggacatg aggagctgcc tcacagaggc tgaggctgca gtggccaccc
     4441 aggagacatc caccgtcagg ctaaaggtca gctccacagc cgtaaggaca cagcacacaa
     4501 ccacccggcc tgttcccgac acctcccggc tgcctggggc cacccctggg ctcaccacgg
     4561 tggagatagt gacaatgtct caccaagctc tgggcgacgt tgctggcaga ggaaatgaga
     4621 agaagcccag tagcgtgagg gctctaagct tggtcctccc catcgtgctc ctcgtcttcc
     4681 tttgcctggg ggtcttcctt ctatggaaga actggcggct taagaacatc aacagcatca
     4741 actttgacaa ccccgtctat cagaagacca cagaggatga ggtccacatt tgccacaacc
     4801 aggacggcta cagctacccc tcgagacaga tggtcagtct ggaggatgac gtggcgtgac
     4861 ggaccgtcta atctagataa gtaatgatca taatcagcca tatcacatct gtagaggttt
     4921 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     4981 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5041 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5101 tcaatgtatc ttatcatgtc tggatccccg ggtaccgagc tcgaattcgt aatcatgtca
     5161 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     5221 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     5281 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     5341 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     5401 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5461 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     5521 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     5581 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     5641 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     5701 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5761 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     5821 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5881 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     5941 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6001 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6061 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6121 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6181 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6241 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     6301 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6361 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6421 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     6481 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     6541 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     6601 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     6661 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     6721 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     6781 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     6841 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     6901 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     6961 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7021 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7081 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7141 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7201 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7261 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     7321 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     7381 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     7441 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     7501 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     7561 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
     7621 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     7681 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     7741 tcccagtcac gacgttgtaa aacgacggcc agtgccaagt tgggatcttt gcattggccc
     7801 acggctctca ggatggggat gctccccttc agcacccggt tccccttgga aactgatggt
     7861 cctggctctg tggcatggca gtggcactgt gaggagcccc ctaccagcag cacacagtgg
     7921 ggttggcact gccacgctcc ggatgccgcg ctctgatcca accccataat caagggaacc
     7981 cgaattgccc catcattgcc cccaccaccc ccatcctgcc gggccctcac accccacgct
     8041 gccttctggt gacattcccc agcccaaacc cacggcttca tggctaccgc ggggcatttc
     8101 ccattgccgc cccattatca gctctgcaca cctcccgctg tacccatgcc tcgtggctgc
     8161 ccttctttga cgtataatct tctaattaat acccggcctt gtcaaagtgg agcacaaacg
     8221 ttaattaatt ccccagcagg caggtaatta acagtgtgac tccctttttg ctgcgagtgg
     8281 ggctgataca gagagatgtg gcactatgga gcccacgggg tcctggcact gggtgcccac
     8341 ggaggtcccc atgtgctgca gtgtcaccgc ctccgaggag acagtattgt ccctgcggtg
     8401 tccctgcagc tcagctctgt ccacagggcc acctccagtt tggaggggac acaatgcagc
     8461 cccgatgcaa cccatcctcg cagcatccca gggacaaaga ccccactgca agaccgcaca
     8521 cagggctggg tcccgctccc ctaatatcta cagtgcttct tgcatggccc cttaatcaat
     8581 gcagttaatc agcatgcgct catgcaccgc tctggagctg caaagcccct cgcagcgctg
     8641 ctcaccaaca ccgcgcaccg ccccggccca gcctgcagca cgcgctgcaa acaggaaaga
     8701 aacaaaatat tgcccaaatg taggcaaagg cattcggctg ccttgacctc cgccgggccg
     8761 ggccctgcct gactcagctc cttactcagc gctcgcttcc tccctccggc tgccaccgcc
     8821 gcagcgcaca ccctgacaaa gagtggccct taacgggctc tgaggtgcac ccagcagtgc
     8881 actcagcagt ccaagggccg gcctggaggt ttgcaccgct acgtgctgac attagcattg
     8941 aacttggccc tgggtagtgc tgcaggccgg gcggggtggg tgtagagagt gcagcgcgcg
     9001 ttgcacccgg tgccccttcc cctcccttgc atcccagcag gctgcacccc agcaccaggc
     9061 ccgtgcatgc atgctcctgg tgttattgca gcctggtgca tgcatgcgtc ttgatggtgc
     9121 agcgctgtgc atgcatcctc cttggtgtgt agcagtctag tgcatgcata cccctcggtg
     9181 ttattgctgc tctgtgcacg cacgctcatt gtatcacttc atcccagtgc atgcactcac
     9241 actggagcga ttgctgctcg gtgcacgcac actcattgta tcactgcagc tcagtggctg
     9301 cacgcacacc ggtgttattg ctgctcggtg cgtgcatgca catcagtgtc gctgcagctc
     9361 agtgcatgca cgctcattgc ccatcgctat ccctgcctct cctgctggcg ctccccggga
     9421 ggtgacttca aggggaccgc aggaccacct cgggggtggg gggagggctg cacacgcgga
     9481 ccccgctccc cctccccaac aaagcactgt ggaatcaaaa aggggggagg ggggatggag
     9541 gggcgcgtca cacccccgcc ccacaccctc acc
//
