LOCUS       sequence-319453-        9766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Smo-FLAG K434R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Desai PB, Stuck MW, Lv B, Pazour GJ
  TITLE     Ubiquitin links smoothened to intraflagellar transport to regulate 
            Hedgehog signaling.
  JOURNAL   J Cell Biol. 2020 Jul 6;219(7). pii: 151798. doi: 
            10.1083/jcb.201912104.
  PUBMED    32435793
REFERENCE   2  (bases 1 to 9766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-07-6"; volume: "219(7). pii: 151798. doi"; 
            pages: " 10.1083/jcb.201912104"
COMMENT     Sequence Label: GP821
FEATURES             Location/Qualifiers
     source          1..9766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    280..405
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    902..1135
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1320..1364
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1660..1777
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    1826..1874
                     /note="synthetic polyadenylation signal"
     misc_feature    1888..1979
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     1928..1947
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    1994..2128
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2031..2050)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             2324..2719
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    join(2720..3293,3294..3296,3297..3305)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2886..2903)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3113..3132
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      5692..5701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5701..5766
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5807..6395
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5860..5880)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6278..6289)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6543..6723
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6770..6788)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6856..6960
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6961..7029,7030..7821)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7179..7198)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7992..8580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8734..8751
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8991..9012
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9027..9032,9033..9050,9051..9057)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9065..9081
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9070..9092
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9089..9105
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9089..9105
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             join(9366..9766,1..233)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
       61 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      121 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      181 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      241 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      301 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tgcagagtac gccaaaaatt
      361 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      421 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      481 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      541 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      601 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      661 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      721 agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata
      781 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
      841 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
      901 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
      961 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     1021 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     1081 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     1141 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1201 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     1261 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     1321 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     1381 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     1441 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     1501 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     1561 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt
     1621 atcgccgaat tctcaacaat aggcagtatt catccacaat tttaaaagaa aaggggggat
     1681 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     1741 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattact cggacagcag
     1801 agatccagtt tggactagtg gccgcaataa aatatcttta ttttcattac atctgtgtgt
     1861 tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca
     1921 aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctcta
     1981 tcgataggta ccgaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     2041 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     2101 catcaatgta tcttatcatg tctggatccc cgggaacaga ttcgcgacta gtggacacac
     2161 aggatagggg tgggcagcat gaggggggcc agagggagag ggggcagagc tgggctggac
     2221 gaggggacac cccgccccaa tggggcgtga cgagctgcca gcccaggctc cggggcacgt
     2281 aaaagcgggg ctgtgagacc ggagagcacg gagcgacatt aatatggcca agcctttgtc
     2341 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
     2401 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
     2461 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
     2521 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
     2581 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
     2641 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
     2701 tggttatgtg tgggagggcc ccctctccct ccccccccct aacgttactg gccgaagccg
     2761 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     2821 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     2881 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     2941 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     3001 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     3061 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     3121 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     3181 tgatctgggg cctcggtaca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     3241 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
     3301 caaccataga ccatggccgc tggccgcccc gtgcgtgggc ccgagctggc gccccggagg
     3361 ctgctgcagt tgctgctgct ggtactgctg gggggcccgg gccggggggc ggccttgagc
     3421 gggaacgtga ccgggcctgg gcctcacagc gccagcggga gctcgaggag ggacgtgccg
     3481 gtgaccagcc ctccgccgcc gctgctgagc cactgcggcc gggccgccca ctgcgagcct
     3541 ttgcgctaca acgtgtgcct gggctcggcg ctgccctacg gagccaccac cacgctgctg
     3601 gctggggact cggactcgca ggaggaagcg cacggcaagc tcgtgctctg gtccggcctc
     3661 cggaatgccc cccgctgctg ggcagtgatc cagcccctgc tgtgtgctgt ctacatgccc
     3721 aagtgtgaga atgaccgagt ggagttgccc agccgtaccc tctgccaggc cacccgaggc
     3781 ccctgtgcca ttgtggagcg ggagcgaggg tggcctgact ttctgcgttg cacaccggac
     3841 cacttccctg aaggctgccc aaacgaggta caaaacatca agttcaacag ctcaggccaa
     3901 tgtgaagcac ccttggtgcg aacagacaac cccaagagct ggtatgagga cgtggagggc
     3961 tgtgggattc agtgtcagaa cccgctgttc accgaggccg agcaccagga catgcacagc
     4021 tacatcgcag ccttcggggc ggtcactggt ctctgcacgc tcttcacact ggccaccttt
     4081 gtggctgact ggcggaactc caatcgctac cctgcggtta ttctcttcta tgtcaatgcg
     4141 tgtttcttcg tgggcagcat tggctggctg gcccagttca tggatggtgc ccgccgagag
     4201 attgtttgcc gagcagatgg caccatgaga tttggggagc ccacctccag tgagaccctg
     4261 tcctgtgtca tcatctttgt cattgtgtac tatgccttga tggctggagt agtctggttc
     4321 gtggtcctca cctatgcctg gcacacctcc ttcaaagccc tgggcaccac ctaccagcct
     4381 ctctcgggca agacatccta tttccacctg ctcacgtggt cactcccctt tgtcctcacg
     4441 gtggcaatcc tggctgtggc tcaggtagat ggagactccg tgagtggcat ctgttttgta
     4501 ggctacaaga actatcggta ccgtgctggc tttgtcctgg ccccaattgg cctggtgctt
     4561 attgtgggag gctacttcct catcagaggg gtcatgactc tgttctccat caggagcaac
     4621 caccctgggc ttctgagtga gaaggcagcc agcaagatca acgagaccat gctgcgcctg
     4681 ggcatttttg gcttcctggc ctttggcttt gtgctcatca ccttcagctg ccacttctat
     4741 gacttcttca accaggctga gtgggagcgt agcttccggg actatgtgct atgccaagcc
     4801 aacgtgacca tcgggctgcc taccaagaag cccattcctg actgtgagat caagaatcgg
     4861 cccagcctcc tggtggagaa gatcaatcta tttgccatgt ttggcactgg cattgccatg
     4921 agcacctggg tctggaccaa ggccaccctg ctcatctgga ggcgcacctg gtgcaggttg
     4981 actgggcaca gtgatgatga gcccaagaga atcaagaaga gcaagatgat cgccaaggcc
     5041 ttctctaagc ggcgtgagct gctgcagaac ccgggccagg agctctcctt cagcatgcac
     5101 actgtctccc atgatggacc tgttgcgggt ttggcttttg acctcaatga accctcagct
     5161 gacgtctcct ctgcctgggc tcagcatgtc accaagatgg tggctcggag aggagccata
     5221 ttgccccagg atgtgtccgt tacccctgtg gcaactccag tgccaccaga agaacaagcc
     5281 aacatgtggc tggttgaggc agagatctcc ccagagttag agaagcgttt gggccggaag
     5341 aaaaagcgga ggaagaggaa gaaggaggtg tgccccttga ggccagcccc tgagcttcac
     5401 cactctgccc ctgttcctgc caccagtgca gttcctcggc tgcctcagct gcctcggcag
     5461 aagtgcctgg tagctgcaaa cgcctgggga acaggggagt cctgccgaca gggagcctgg
     5521 actctagtct ccaacccctt ctgcccagag cctagtcccc atcaagatcc atttctccct
     5581 ggtgcctcag ccccccgggt ctgggctcag ggccgcctcc aggggctggg atccattcat
     5641 tcccgcacta acctaatgga ggctgagatc ttggatgcag actcggactt cgccaccatg
     5701 gactacaaag accatgacgg tgattataaa gatcatgaca tcgattacaa ggatgacgat
     5761 gacaaggcgg ccgcttagta agtaagatcc gaactcgaga gatcctaatc aacctctgga
     5821 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5881 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5941 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6001 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6061 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6121 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6181 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     6241 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     6301 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     6361 gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt
     6421 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     6481 ttcactccca acgaagacaa gatcacctgc aggacaggcg cgccctgctt tttgcttgta
     6541 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6601 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6661 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6721 gcacccgggc gattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata
     6781 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     6841 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6901 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6961 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     7021 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     7081 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     7141 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     7201 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     7261 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     7321 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     7381 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     7441 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     7501 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     7561 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     7621 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     7681 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     7741 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     7801 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     7861 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7921 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7981 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     8041 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     8101 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     8161 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     8221 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     8281 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     8341 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     8401 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8461 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8521 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8581 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8641 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     8701 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     8761 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag
     8821 ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8881 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg
     8941 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     9001 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     9061 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc
     9121 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     9181 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     9241 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     9301 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     9361 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     9421 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     9481 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     9541 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     9601 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     9661 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     9721 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggg
//
