LOCUS       sequence-31946-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Johnson LR, Johnson TK, Desler M, Luster TA, Nowling T, Lewis RE, 
            Rizzino A
  TITLE     Effects of B-Myb on gene transcription: phosphorylation-dependent 
            activity ans acetylation by p300.
  JOURNAL   J Biol Chem. 2002 Feb 8. 277(6):4088-97.
  PUBMED    11733503
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2002 Feb 8. 277(6):4088-97."
COMMENT     Sequence Label: pCDNA3 14 mut B-Myb
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      126..135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             135..158
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctctctggct actgagaacc cactgcttac tggcttatcg aaattaatac gactcactat
       61 agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg
      121 aattcgccac catggactac aaggacgacg atgacaagtc tcggcggacg cgctgcgagg
      181 atctggatga gttacactac caggacgtgg actcagacct cctggagcag agagacaaca
      241 gatgtaaggt taaatggacc cacgaggagg acgagcagtt gagggccctg gtgaggcagt
      301 ttggacagca agactggaag ttcttggcca gtcactttcc taaccgcaca gaccagcaat
      361 gccagtaccg gtggctgagg gttttgaatc ccgaccttgt taagggacca tggaccaaag
      421 aggaagatca aaaggtcatt gagttggtca agaagtatgg caccaaacag tggacgctga
      481 tagccaagca cctgaagggc cggctgggga agcagtgccg tgagcgctgg cacaaccacc
      541 tcaaccccga ggtgaagaag tcatgctgga ccgaggagga agacaggata atctgtgagg
      601 cccataaagt cctgggtaac cgctgggctg agatcgccaa gatgctacca gggaggacgg
      661 acaatgctgt gaagaatcac tggaactcca ctatcaaaag gaaagtcgac acgggaggtt
      721 tcccagccga gtccagggac tgcaagcctg tctacttgct cctggagctg gaggacaagg
      781 aacagcacca gggtgtccag ccggtggacg gccagggaag tctcgtgagc agctggccgc
      841 tggtgccctc tattgtgaag gaggagagca gcgagaagga gattgccata gctgctactt
      901 ctgctaaaga actcggacat gancctgtcc ctgctgatct gggagaagtg cgcgttccgg
      961 aaccccc
//
