LOCUS       sequence-319469-       13690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration Vector, assembled with pHIE049 (TUPV1), pJM466 (TUPV2), 
            pJM452 (TUPV3), pPD613.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13690)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 13690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pHIE057 ZF43x12-S mKate2 EF1a VP16-ZF43 EF1a
            E...
FEATURES             Location/Qualifiers
     source          1..13690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1031..1068
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1079..1678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1079..1098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1575..1595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1791..1810)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1856..1911
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1910..1929)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     regulatory      3380..3389
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3386..4084
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    4135..4359
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5408..6590
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5639..6581
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      6691..6700
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6700..6765
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6772..6792
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7389..7613
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8662..9844
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8893..9835
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      9945..9954
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9951..9953,9954..9956,9957..10667)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(9996..10017)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             10695..10751
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10764..11162
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    11213..11437
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(12296..13105)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     13019..13038
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     13097..13116
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(join(13264..13690,1..162))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13344..13363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
       61 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      241 ttatcaaaaa ggatcttcac ctagatcctt tctcgagctt tcggagtgag atggagtggc
      301 cactgtggaa ctgggcctag catcgcgata agctctagag ggacagcccc cccccaaagc
      361 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
      421 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
      481 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
      541 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
      601 cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt gatatcgaat
      661 tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
      721 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
      781 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
      841 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
      901 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
      961 gcgcgcctgg tggcgaacga tcaacagacg ggagatctac tattgggaca gcagagatcc
     1021 agttggtaat ggcttgtcga cgacggcggt ctccgtcgtc aggatcatag gtgccaccat
     1081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     1141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     1201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1561 gacatccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc
     1681 ttccactgga ttgtacaatt accaactgaa ttcactcctc aggtgcaggc tgcctatcag
     1741 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     1801 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     1861 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     1921 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     1981 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     2041 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     2101 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     2161 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     2221 cttctcttat ggagatccgg gcctagcatc gcgataagct ctagagggac agcccccccc
     2281 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     2341 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     2401 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     2461 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     2521 gaatgacaga atcatagaac ggcctgcgta cggatggggc tctagctcta gagcttgata
     2581 tcgaattcct gcagccccgc tagagggaca gccccccccc aaagccccca gggatgtaat
     2641 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     2701 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     2761 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     2821 ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg
     2881 gcctgggcgc gcctggtggc gaacgatcaa cagacgggag attgccaagg tcttgacaaa
     2941 cgagtgctag tacaagatct gaattcggat ccagagtgag gactacctat agaagtaaga
     3001 gtgaggacaa tcgtatacaa gtagatccag agtgaggact acctatagaa gtaagagtga
     3061 ggacaatcgt atacaagtag atccagagtg aggactacct atagaagtaa gagtgaggac
     3121 aatcgtatac aagtagatcc ctctagaggg agatccagag tgaggactac ctatagaagt
     3181 aagagtgagg acaatcgtat acaagtagat ccagagtgag gactacctat agaagtaaga
     3241 gtgaggacaa tcgtatacaa gtagatccag agtgaggact acctatagaa gtaagagtga
     3301 ggacaatcgt atacaagtag atccctctag agggtatata atgggggcca ctagtctact
     3361 accagagctc atcgctagcg ccaccatggt gagcgagctg attaaggaga acatgcacat
     3421 gaagctgtat atggagggca ccgtgaacaa ccaccacttc aagtgtacct ccgagggcga
     3481 aggcaagccc tacgagggca cccagaccat gagaatcaag gcggtcgagg gcggccctct
     3541 ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcaaaa ccttcatcaa
     3601 ccacacccag ggcatccccg acttctttaa gcagtccttc cccgagggct tcacatggga
     3661 gagagtcacc acatacgaag atgggggcgt gctgaccgct acccaggaca ccagcctcca
     3721 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcccat ccaacggccc
     3781 tgtgatgcag aagaaaacac tcggctggga ggcctccacc gaaaccctgt accccgctga
     3841 cggcggcctg gaaggcagag ccgacatggc cctgaagctc gtgggcgggg gccacctgat
     3901 ctgcaacttg aaaaccacat acagatccaa gaaacccgct aagaacctca agatgcccgg
     3961 cgtctactat gtggacagga gactggaaag aatcaaggag gccgacaaag aaacctacgt
     4021 cgagcagcac gaggtggctg tggccagata ctgcgacctc cctagcaaac tggggcacag
     4081 atgagcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     4141 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4201 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     4261 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4321 tcaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggca ggtcgagggt
     4381 acatgcgcct tactccttgt gagcgcatcg cgataagctc tagagggaca gccccccccc
     4441 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
     4501 gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag
     4561 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
     4621 gagcctgcag acacctgggg ggatacgggg aaaaagcttt aggctgaaag agagatttag
     4681 aatgacagaa tcatagaacg gcctgcgtac ggatggggct ctagctctag agcttgatat
     4741 cgaattcctg cagccccgct agagggacag ccccccccca aagcccccag ggatgtaatt
     4801 acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg
     4861 ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc
     4921 acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg
     4981 gatacgggga aaaagcttta ggctgaaaga gagatttaga atgacagaat catagaacgg
     5041 cctgggcgcg cctggtggcg aacgatcaac agacgggaga gggcaaaacc gatgttgacg
     5101 gactaatcct gacaagatct ggggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     5161 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     5221 cgctgcttcg cgatgtacgg gccagatata cgcgtgagta attcatacaa aaggactcgc
     5281 ccctgccttg gggaatccca gggaccgtcg ttaaactccc actaacgtag aacccagaga
     5341 tcgctgcgtt cccgccccct cacccgcccg ctctcgtcat cactgaggtg gagaagagca
     5401 tgcgtgaggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     5461 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     5521 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     5581 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     5641 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
     5701 ttgaattact tccacgcccc tggctgcagt acgtgattct tgatcccgag cttcgggttg
     5761 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
     5821 ttgaggcctg gcttgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
     5881 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
     5941 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
     6001 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
     6061 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg
     6121 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
     6181 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
     6241 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
     6301 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca
     6361 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
     6421 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
     6481 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
     6541 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga gagctcgttt
     6601 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaga
     6661 ccgggaccga tccaaattct cgaggctagc gccaccatgg attataagga tcacgatggc
     6721 gactacaagg accacgatat tgactacaaa gacgatgacg ataaaatggc tcctaagaaa
     6781 aagcgcaaag tcggtatcca tggcgttccc gtcgacggtg gactgcaggc cccaccaacc
     6841 gatgtttctt taggtgatga gttacatttg gacggtgagg acgtagctat ggcacatgca
     6901 gatgccttag acgattttga cttggatatg ttaggagatg gagattcacc aggacctggt
     6961 ttcacaccac acgattcagc tccatacggt gctttggaca tggccgattt cgaatttgaa
     7021 cagatgttta ctgatgcctt gggtatagac gaatacggtg gtggagctag acccggggag
     7081 cgccccttcc agtgtcgcat ttgcatgcgg aacttttcgc gccaggacag gcttgacagg
     7141 catacccgta ctcataccgg tgaaaaaccg tttcagtgtc ggatctgtat gcgaaatttc
     7201 tcccagaagg agcacttggc ggggcatcta cgtacgcaca ccggcgagaa gccattccaa
     7261 tgccgaatat gcatgcgcaa cttcagtcgc cgcgacaacc tgaaccggca cctaaaaacc
     7321 cacctgagag gatcttaagc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc
     7381 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     7441 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     7501 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     7561 ggaggattgg gaagtcaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     7621 ggcaggtcga agaactattt cctggctgtt acgcgagcgc atcgcgataa gctctagagg
     7681 gacagccccc ccccaaagcc cccagggatg taattacgtc cctcccccgc tagggggcag
     7741 cagcgagccg cccggggctc cgctccggtc cggcgctccc cccgcatccc cgagccggca
     7801 gcgtgcgggg acagcccggg cacggggaag gtggcacggg atcgctttcc tctgaacgct
     7861 tctcgctgct ctttgagcct gcagacacct ggggggatac ggggaaaaag ctttaggctg
     7921 aaagagagat ttagaatgac agaatcatag aacggcctgc gtacggatgg ggctctagct
     7981 ctagagcttg atatcgaatt cctgcagccc cgctagaggg acagcccccc cccaaagccc
     8041 ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc
     8101 gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc
     8161 acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg
     8221 cagacacctg gggggatacg gggaaaaagc tttaggctga aagagagatt tagaatgaca
     8281 gaatcataga acggcctggg cgcgcctggt ggcgaacgat caacagacgg gagaggacta
     8341 aatagtagtt cagacgccgt taagcgcaag atctggggag gtcgctgagt agtgcgcgag
     8401 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     8461 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtg agtaattcat
     8521 acaaaaggac tcgcccctgc cttggggaat cccagggacc gtcgttaaac tcccactaac
     8581 gtagaaccca gagatcgctg cgttcccgcc ccctcacccg cccgctctcg tcatcactga
     8641 ggtggagaag agcatgcgtg aggctccggt gcccgtcagt gggcagagcg cacatcgccc
     8701 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg
     8761 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     8821 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     8881 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat
     8941 ggcccttgcg tgccttgaat tacttccacg cccctggctg cagtacgtga ttcttgatcc
     9001 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
     9061 gcctcgtgct tgagttgagg cctggcttgg gcgctggggc cgccgcgtgc gaatctggtg
     9121 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
     9181 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
     9241 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
     9301 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
     9361 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
     9421 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
     9481 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
     9541 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     9601 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
     9661 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     9721 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     9781 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     9841 gtgagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
     9901 ctccatagaa gagaacggga ccgatccaaa ttctcgaggc tagcgccacc atggtgagca
     9961 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
    10021 acggccacaa gttcagcgtg aggggcgagg gcgagggcga tgccaccaac ggcaagctga
    10081 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
    10141 ccctgagcca cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
    10201 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
    10261 acggcaccta caagacccgc gccgaggtga agttcgaggg cgacacccta gtgaaccgca
    10321 tcgagctgaa gggcgtcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
    10381 acaacttcaa cagccacaac atctatatca tggccgtcaa gcagaagaac ggcatcaagg
    10441 tgaacttcaa gatccgccac aacgtggagg acggcagcgt gcagctcgcc gaccactacc
    10501 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacagccac tacctgagca
    10561 cccagtccgt gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
    10621 tccgcaccgc cgccgggatc actctcggca tggacgagct gtacaaggaa ttcggtagca
    10681 agcttggtag tggagcaact aacttttccc tgctcaaaca agccggggat gtagaggaga
    10741 accccggccc cgcccccctt aagatggcca agcctttgtc tcaagaagaa tccaccctca
    10801 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaggactac agcgtcgcca
    10861 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
    10921 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
    10981 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
    11041 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
    11101 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
    11161 aagcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
    11221 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
    11281 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
    11341 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagtc
    11401 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcagg tcgaagagac
    11461 tgcccgacac atcttagtga gcgcatcgcg ataagctcta gagggacagc ccccccccaa
    11521 agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg
    11581 gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc ggggacagcc
    11641 cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga
    11701 gcctgcagac acctgggggg atacggggaa aaagctttag gctgaaagag agatttagaa
    11761 tgacagaatc atagaacggc ctgcgtacgg atggggctct agctctagag cttgatatcg
    11821 aattcctgca gccccgctag agggacagcc cccccccaaa gcccccaggg atgtaattac
    11881 gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct
    11941 ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac
    12001 gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga
    12061 tacggggaaa aagctttagg ctgaaagaga gatttagaat gacagaatca tagaacggcc
    12121 tgggcgcgcc tggtggcgaa cgatcaacag acgggagagg ttacgaggat gcacatgtga
    12181 ccgagggatg gaacgacgcg taactcacgt taagggattt tggtcatgag cttgcgccgt
    12241 cccgtcaagt cagcgtaatg ctctgccagt gttacaacca attaaccaat tctgattaga
    12301 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
    12361 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
    12421 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
    12481 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
    12541 ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat
    12601 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
    12661 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
    12721 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
    12781 ctaatacctg gaatgctgtt tttccgggga tcgcagtggt gagtaaccat gcatcatcag
    12841 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
    12901 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
    12961 ctggcgcatc gggcttccca tacaagcgat agattgtcgc acctgattgc ccgacattat
    13021 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg
    13081 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
    13141 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
    13201 acacgggcca tgctagctcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    13261 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    13321 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    13381 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    13441 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    13501 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    13561 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    13621 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    13681 gcctaactac
//
