LOCUS       sequence-319470-       11963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration Vector, assembled with pHIE041 (TUPV1), pJM451 (TUPV2), 
            pPD612.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11963)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 11963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pHIE067 CAG mKate2 EF1a EBFP2-P2A-BlastR
FEATURES             Location/Qualifiers
     source          1..11963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1031..1068
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1079..1678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1079..1098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1575..1595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1791..1810)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1856..1911
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1910..1929)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        3097..3476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3752..4768
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4692..4714
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4776..4795
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     4869..4888
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4869..4887
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4907..4916
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4913..5611
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    5662..5886
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        6935..8117
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7166..8108
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8065..8085
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     8119..8143
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      8218..8227
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8224..8226,8227..8229,8230..8940)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(8269..8290)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             8968..9024
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9037..9435
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    9486..9710
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(10569..11378)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     11292..11311
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     11370..11389
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(join(11537..11963,1..162))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11617..11636)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
       61 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      241 ttatcaaaaa ggatcttcac ctagatcctt tctcgagctt tcggagtgag atggagtggc
      301 cactgtggaa ctgggcctag catcgcgata agctctagag ggacagcccc cccccaaagc
      361 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
      421 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
      481 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
      541 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
      601 cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt gatatcgaat
      661 tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
      721 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
      781 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
      841 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
      901 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
      961 gcgcgcctgg tggcgaacga tcaacagacg ggagatctac tattgggaca gcagagatcc
     1021 agttggtaat ggcttgtcga cgacggcggt ctccgtcgtc aggatcatag gtgccaccat
     1081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     1141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     1201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1561 gacatccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc
     1681 ttccactgga ttgtacaatt accaactgaa ttcactcctc aggtgcaggc tgcctatcag
     1741 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     1801 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     1861 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     1921 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     1981 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     2041 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     2101 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     2161 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     2221 cttctcttat ggagatccgg gcctagcatc gcgataagct ctagagggac agcccccccc
     2281 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     2341 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     2401 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     2461 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     2521 gaatgacaga atcatagaac ggcctgcgta cggatggggc tctagctcta gagcttgata
     2581 tcgaattcct gcagccccgc tagagggaca gccccccccc aaagccccca gggatgtaat
     2641 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     2701 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     2761 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     2821 ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg
     2881 gcctgggcgc gcctggtggc gaacgatcaa cagacgggag attgccaagg tcttgacaaa
     2941 cgagtgctag tacaagatct ggggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     3001 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     3061 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
     3121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     3181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     3241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     3301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     3361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     3421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg
     3481 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
     3541 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggsgsgc
     3601 gccrggcggg gsggggsggg gsgrggggsg gggcggggcg aggcggagag gtgcggcggc
     3661 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg
     3721 gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt
     3781 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
     3841 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg
     3901 acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt
     3961 gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
     4021 cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct
     4081 ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg
     4141 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
     4201 cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc
     4261 ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg
     4321 gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg
     4381 ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
     4441 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc
     4501 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg
     4561 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc
     4621 ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg
     4681 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
     4741 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
     4801 tcatcatttt ggcaaagaat tcttatctgg ctaactagag aacccactgc ttactggctt
     4861 atcgaaatta atacgactca ctatagggag tcccaagctg gctagcgcca ccatggtgag
     4921 cgagctgatt aaggagaaca tgcacatgaa gctgtatatg gagggcaccg tgaacaacca
     4981 ccacttcaag tgtacctccg agggcgaagg caagccctac gagggcaccc agaccatgag
     5041 aatcaaggcg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt
     5101 catgtacggc agcaaaacct tcatcaacca cacccagggc atccccgact tctttaagca
     5161 gtccttcccc gagggcttca catgggagag agtcaccaca tacgaagatg ggggcgtgct
     5221 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag
     5281 aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
     5341 ctccaccgaa accctgtacc ccgctgacgg cggcctggaa ggcagagccg acatggccct
     5401 gaagctcgtg ggcgggggcc acctgatctg caacttgaaa accacataca gatccaagaa
     5461 acccgctaag aacctcaaga tgcccggcgt ctactatgtg gacaggagac tggaaagaat
     5521 caaggaggcc gacaaagaaa cctacgtcga gcagcacgag gtggctgtgg ccagatactg
     5581 cgacctccct agcaaactgg ggcacagatg agcggccgct cgagtctaga gggcccgttt
     5641 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     5701 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     5761 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     5821 aggacagcaa gggggaggat tgggaagtca atagcaggca tgctggggat gcggtgggct
     5881 ctatggcttc tgaggcaggt cgagggtaca tgcgccttac tccttgtgag cgcatcgcga
     5941 taagctctag agggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc
     6001 cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat
     6061 ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt
     6121 tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa
     6181 aagctttagg ctgaaagaga gatttagaat gacagaatca tagaacggcc tgcgtacgga
     6241 tggggctcta gctctagagc ttgatatcga attcctgcag ccccgctaga gggacagccc
     6301 ccccccaaag cccccaggga tgtaattacg tccctccccc gctagggggc agcagcgagc
     6361 cgcccggggc tccgctccgg tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg
     6421 ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg cttctcgctg
     6481 ctctttgagc ctgcagacac ctggggggat acggggaaaa agctttaggc tgaaagagag
     6541 atttagaatg acagaatcat agaacggcct gggcgcgcct ggtggcgaac gatcaacaga
     6601 cgggagaggg caaaaccgat gttgacggac taatcctgac aagatctggg gaggtcgctg
     6661 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     6721 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     6781 gtgagtaatt catacaaaag gactcgcccc tgccttgggg aatcccaggg accgtcgtta
     6841 aactcccact aacgtagaac ccagagatcg ctgcgttccc gccccctcac ccgcccgctc
     6901 tcgtcatcac tgaggtggag aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga
     6961 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
     7021 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     7081 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     7141 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
     7201 ctttacgggt tatggccctt gcgtgccttg aattacttcc acgcccctgg ctgcagtacg
     7261 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     7321 aaggagcccc ttcgcctcgt gcttgagttg aggcctggct tgggcgctgg ggccgccgcg
     7381 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     7441 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     7501 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     7561 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     7621 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     7681 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     7741 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     7801 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     7861 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     7921 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     7981 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     8041 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     8101 catttcaggt gtcgtgagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
     8161 acgctgtttt gacctccata gaagagaacg ggaccgatcc aaattctcga ggctagcgcc
     8221 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     8281 gacggcgacg taaacggcca caagttcagc gtgaggggcg agggcgaggg cgatgccacc
     8341 aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     8401 accctcgtga ccaccctgag ccacggcgtg cagtgcttcg cccgctaccc cgaccacatg
     8461 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     8521 ttcttcaagg acgacggcac ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     8581 ctagtgaacc gcatcgagct gaagggcgtc gacttcaagg aggacggcaa catcctgggg
     8641 cacaagctgg agtacaactt caacagccac aacatctata tcatggccgt caagcagaag
     8701 aacggcatca aggtgaactt caagatccgc cacaacgtgg aggacggcag cgtgcagctc
     8761 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacagc
     8821 cactacctga gcacccagtc cgtgctgagc aaagacccca acgagaagcg cgatcacatg
     8881 gtcctgctgg agttccgcac cgccgccggg atcactctcg gcatggacga gctgtacaag
     8941 gaattcggta gcaagcttgg tagtggagca actaactttt ccctgctcaa acaagccggg
     9001 gatgtagagg agaaccccgg ccccgccccc cttaagatgg ccaagccttt gtctcaagaa
     9061 gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaggac
     9121 tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta
     9181 tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg
     9241 gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc
     9301 ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg
     9361 aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat
     9421 gtgtgggagg gctaagcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc
     9481 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     9541 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     9601 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     9661 ggattgggaa gtcaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     9721 aggtcgaaga actatttcct ggctgttacg cgagcgcatc gcgataagct ctagagggac
     9781 agcccccccc caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag
     9841 cgagccgccc ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg
     9901 tgcggggaca gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct
     9961 cgctgctctt tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa
    10021 gagagattta gaatgacaga atcatagaac ggcctgcgta cggatggggc tctagctcta
    10081 gagcttgata tcgaattcct gcagccccgc tagagggaca gccccccccc aaagccccca
    10141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
    10201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
    10261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
    10321 acacctgggg ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa
    10381 tcatagaacg gcctgggcgc gcctggtggc gaacgatcaa cagacgggag aggactagag
    10441 gatgcacatg tgaccgaggg atggaacgac gcgtaactca cgttaaggga ttttggtcat
    10501 gagcttgcgc cgtcccgtca agtcagcgta atgctctgcc agtgttacaa ccaattaacc
    10561 aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
    10621 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
    10681 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
    10741 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
    10801 gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca
    10861 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
    10921 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca
    10981 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
    11041 tcaggatatt cttctaatac ctggaatgct gtttttccgg ggatcgcagt ggtgagtaac
    11101 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
    11161 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
    11221 ttcagaaaca actctggcgc atcgggcttc ccatacaagc gatagattgt cgcacctgat
    11281 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
    11341 aatcgcggcc tcgacgtttc ccgttgaata tggctcataa caccccttgt attactgttt
    11401 atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat
    11461 cagagatttt gagacacggg ccatgctagc tccagcaaaa ggccaggaac cgtaaaaagg
    11521 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    11581 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    11641 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    11701 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    11761 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    11821 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    11881 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    11941 tcttgaagtg gtggcctaac tac
//
