LOCUS       sequence-319472-        7719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of rat BDNF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Frank M, Citarella CG, Quinones GB, Bentley M
  TITLE     A Novel Labeling Strategy Reveals That Myosin Va and Myosin Vb Bind 
            the Same Dendritically Polarized Vesicle Population.
  JOURNAL   Traffic. 2020 Sep 21. doi: 10.1111/tra.12764.
  PUBMED    32959500
REFERENCE   2  (bases 1 to 7719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2020 Sep 21. doi: 10.1111/tra.12764."
COMMENT     Sequence Label: pBa. BDNF-Halo
FEATURES             Location/Qualifiers
     source          1..7719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..276
                     /label=chicken beta-actin promoter
     primer_bind     1219..1241
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             2112..2996
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     polyA_signal    3138..3272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3175..3194)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3229..3248
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3303..3319)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3303..3319)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3316..3338)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3327..3343
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3351..3357,3358..3375,3376..3381))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3396..3417
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3534..3551)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3705..4293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3785..3804)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4464..5255,5256..5324))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5087..5106
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5325..5429)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5497..5515
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5553..5575)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5675..5694
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5888..5910
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5902..5919
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5903..5919
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      121 cgcgcgccrg gsggggcggg gcggggcgrg gggcggggcg gggcgaggcg gagaggtgcg
      181 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      241 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      301 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      361 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      421 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
      481 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
      541 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
      601 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
      661 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
      721 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
      781 acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg
      841 gcggggggkg gcggcaggkg ggggtgccgg gcggggcggg gccgcctcgg gccggggrgg
      901 gctcgggggr ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
      961 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     1021 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     1081 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     1141 gccgtcccct tctccatctc cagcctcggg gctgtccgca gggggacggc tgccttcggg
     1201 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggggtt tatatcttcc
     1261 cttctctgtt cctccgcagc ccccaagctt ggcgcgccac catgaccatc cttttcctta
     1321 ctatggttat ttcatacttc ggttgcatga aggctgcgcc catgaaagaa gcaaacgtcc
     1381 acggacaagg caacttggcc tacccagctg tgcggaccca tgggactctg gagagcgtga
     1441 atgggcccag ggcaggttcg agaggtctga cgacgacgtc cctggctgac acttttgagc
     1501 acgtgatcga agagctgctg gatgaggacc agaaggttcg gcccaacgaa gaaaaccata
     1561 aggacgcgga cttgtacact tcccgggtga tgctcagcag tcaagtgcct ttggagcctc
     1621 ctctgctctt tctgctggag gaatacaaaa attacctgga tgccgcaaac atgtctatga
     1681 gggttcggcg ccactccgac cccgcccgcc gtggggagct gagcgtgtgt gacagtatta
     1741 gcgagtgggt cacagcggca gataaaaaga ctgcagtgga catgtccggt gggacggtca
     1801 cagtcctgga gaaagtcccg gtatcaaaag gccaactgaa gcaatatttc tacgagacca
     1861 agtgtaatcc catgggttac acgaaggaag gctgcagggg catagacaaa aggcactgga
     1921 actcgcaatg ccgaactacc caatcgtatg ttcgggccct tactatggat agcaaaaaga
     1981 gaattggctg gcggttcata aggatagaca cttcctgtgt atgtacactg accattaaaa
     2041 ggggaagagg aggagggcct aggggaggag gttccggagg cggctctggc ggaggggcta
     2101 gcggaggatc cgaaatcggt actggctttc cattcgaccc ccattatgtg gaagtcctgg
     2161 gcgagcgcat gcactacgtc gatgttggtc cgcgcgatgg cacccctgtg ctgttcctgc
     2221 acggtaaccc gacctcctcc tacgtgtggc gcaacatcat cccgcatgtt gcaccgaccc
     2281 atcgctgcat tgctccagac ctgatcggta tgggcaaatc cgacaaacca gacctgggtt
     2341 atttcttcga cgaccacgtc cgcttcatgg atgccttcat cgaagccctg ggtctggaag
     2401 aggtcgtcct ggtcattcac gactggggct ccgctctggg tttccactgg gccaagcgca
     2461 atccagagcg cgtcaaaggt attgcattta tggagttcat ccgccctatc ccgacctggg
     2521 acgaatggcc agaatttgcc cgcgagacct tccaggcctt ccgcaccacc gacgtcggcc
     2581 gcaagctgat catcgatcag aacgttttta tcgagggtac gctgccgatg ggtgtcgtcc
     2641 gcccgctgac tgaagtcgag atggaccatt accgcgagcc gttcctgaat cctgttgacc
     2701 gcgagccact gtggcgcttc ccaaacgagc tgccaatcgc cggtgagcca gcgaacatcg
     2761 tcgcgctggt cgaagaatac atggactggc tgcaccagtc ccctgtcccg aagctgctgt
     2821 tctggggcac cccaggcgtt ctgatcccac cggccgaagc cgctcgcctg gccaaaagcc
     2881 tgcctaactg caaggctgtg gacatcggcc cgggtctgaa tctgctgcaa gaagacaacc
     2941 cggacctgat cggcagcgag atcgcgcgct ggctgtctac tctggagatt tccgggctgt
     3001 acaagcggac cgtctaatct agataagtaa tgatcataat cagccatatc acatctgtag
     3061 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     3121 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3181 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3241 aactcatcaa tgtatcttat catgtctgga tccccgggta ccgagctcga attcgtaatc
     3301 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     3361 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     3421 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     3481 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     3541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3601 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4141 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4741 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4801 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4861 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4921 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4981 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5041 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5101 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5161 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5221 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5281 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5341 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5401 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     5461 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     5521 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     5581 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     5641 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     5701 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     5761 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     5821 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     5881 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagttggg atctttgcat
     5941 tggcccacgg ctctcaggat ggggatgctc cccttcagca cccggttccc cttggaaact
     6001 gatggtcctg gctctgtggc atggcagtgg cactgtgagg agccccctac cagcagcaca
     6061 cagtggggtt ggcactgcca cgctccggat gccgcgctct gatccaaccc cataatcaag
     6121 ggaacccgaa ttgccccatc attgccccca ccacccccat cctgccgggc cctcacaccc
     6181 cacgctgcct tctggtgaca ttccccagcc caaacccacg gcttcatggc taccgcgggg
     6241 catttcccat tgccgcccca ttatcagctc tgcacacctc ccgctgtacc catgcctcgt
     6301 ggctgccctt ctttgacgta taatcttcta attaataccc ggccttgtca aagtggagca
     6361 caaacgttaa ttaattcccc agcaggcagg taattaacag tgtgactccc tttttgctgc
     6421 gagtggggct gatacagaga gatgtggcac tatggagccc acggggtcct ggcactgggt
     6481 gcccacggag gtccccatgt gctgcagtgt caccgcctcc gaggagacag tattgtccct
     6541 gcggtgtccc tgcagctcag ctctgtccac agggccacct ccagtttgga ggggacacaa
     6601 tgcagccccg atgcaaccca tcctcgcagc atcccaggga caaagacccc actgcaagac
     6661 cgcacacagg gctgggtccc gctcccctaa tatctacagt gcttcttgca tggcccctta
     6721 atcaatgcag ttaatcagca tgcgctcatg caccgctctg gagctgcaaa gcccctcgca
     6781 gcgctgctca ccaacaccgc gcaccgcccc ggcccagcct gcagcacgcg ctgcaaacag
     6841 gaaagaaaca aaatattgcc caaatgtagg caaaggcatt cggctgcctt gacctccgcc
     6901 gggccgggcc ctgcctgact cagctcctta ctcagcgctc gcttcctccc tccggctgcc
     6961 accgccgcag cgcacaccct gacaaagagt ggcccttaac gggctctgag gtgcacccag
     7021 cagtgcactc agcagtccaa gggccggcct ggaggtttgc accgctacgt gctgacatta
     7081 gcattgaact tggccctggg tagtgctgca ggccgggcgg ggtgggtgta gagagtgcag
     7141 cgcgcgttgc acccggtgcc ccttcccctc ccttgcatcc cagcaggctg caccccagca
     7201 ccaggcccgt gcatgcatgc tcctggtgtt attgcagcct ggtgcatgca tgcgtcttga
     7261 tggtgcagcg ctgtgcatgc atcctccttg gtgtgtagca gtctagtgca tgcatacccc
     7321 tcggtgttat tgctgctctg tgcacgcacg ctcattgtat cacttcatcc cagtgcatgc
     7381 actcacactg gagcgattgc tgctcggtgc acgcacactc attgtatcac tgcagctcag
     7441 tggctgcacg cacaccggtg ttattgctgc tcggtgcgtg catgcacatc agtgtcgctg
     7501 cagctcagtg catgcacgct cattgcccat cgctatccct gcctctcctg ctggcgctcc
     7561 ccgggaggtg acttcaaggg gaccgcagga ccacctcggg ggtgggggga gggctgcaca
     7621 cgcggacccc gctccccctc cccaacaaag cactgtggaa tcaaaaaggg gggagggggg
     7681 atggaggggc gcgtcacacc cccgccccac accctcacc
//
