LOCUS       sequence-319474-       10836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of chicken NgCam.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10836)
  AUTHORS   Frank M, Citarella CG, Quinones GB, Bentley M
  TITLE     A Novel Labeling Strategy Reveals That Myosin Va and Myosin Vb Bind 
            the Same Dendritically Polarized Vesicle Population.
  JOURNAL   Traffic. 2020 Sep 21. doi: 10.1111/tra.12764.
  PUBMED    32959500
REFERENCE   2  (bases 1 to 10836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2020 Sep 21. doi: 10.1111/tra.12764."
COMMENT     Sequence Label: pBa.NgCAM-Halo
FEATURES             Location/Qualifiers
     source          1..10836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..291
                     /label=chicken beta-actin promoter
     primer_bind     1234..1256
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             5208..5228
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             5244..6128
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     polyA_signal    6270..6404
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6307..6326)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6361..6380
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6435..6451)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6435..6451)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6448..6470)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6459..6475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6483..6489,6490..6507,6508..6513))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6528..6549
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6666..6683)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6837..7425)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6917..6936)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7596..8387,8388..8456))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8219..8238
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8457..8561)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8629..8647
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8685..8707)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8807..8826
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9020..9042
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9034..9051
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9035..9051
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccccacaccc tcacctcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
       61 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
      121 cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg
      181 aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
      241 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct
      301 gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
      361 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
      421 aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga aagccttaaa
      481 gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt
      541 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg
      601 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc
      661 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg
      721 ggggtgagca gggggtgtgg gcgcggcggt cgggctgtaa cccccccctg cacccccctc
      781 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtgcgggg cgtggcgcgg
      841 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
      901 ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc
      961 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     1021 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     1081 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     1141 gtgcgtcgcc gcgccgccgt ccccttctcc atctccagcc tcggggctgt ccgcaggggg
     1201 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1261 gggtttatat cttcccttct ctgttcctcc gcagccccca agcttccgcc atggctctgc
     1321 cccatgggct cggcctcctc ctgctcctgt tgctgggggg gcccggagcc gccatcacca
     1381 ttcccccgga gtatggtgca cacgatttcc tgcagccccc cgagctgacg gaggaacccc
     1441 cggaacaact cgtggtcttc cccagtgatg acatcgtcct caaatgcgtg gccaccggga
     1501 acccccccgt ccagtaccga tggagccgtg aggatcagcc cttcgtcccc gaggagcacg
     1561 ggggggtctc ggtggtcccc ggatcgggga ctttggtcat caacgccacg ttggccgcgc
     1621 ggctccaggg gcgcttccgc tgcttcgcca ccaacgcgtt gggcaccgct gtgtctcccg
     1681 aggccaacgt catcgccgag aacactccgc agtggccgaa ggagaaggtg accccggtgg
     1741 aggtggagga gggggacccc gtggtgctgc cctgtgaccc ccccgagagc gctgtgcccc
     1801 ctaaaatcta ttggctcaac agcgacatcg ttcacatcgc tcaggacgag agggtatcga
     1861 tggggcagga tgggaacctc tacttctcca acgccatggt gggcgacagc caccccgact
     1921 acatctgcca cgctcacttc ctcggccccc gcaccatcat ccagaaggag cccctcgacc
     1981 tccgcgtggc ccccagtaat gcggttcggt cccgccgccc ccgcctgctg ctgccccgcg
     2041 acccccaaac gaccaccatc gccctccggg ggggcagcgt cgtgttggag tgcatcgctg
     2101 aggggctccc cactccatgg gtccgatggc ggcggctgaa tggccccctc cccacccggg
     2161 cggcgttgga gaacttcaac aaaacgctgc ggctgtgggg ggtgacggag agcgacgacg
     2221 gggagtacga atgtgtggct gagaacggga gggggacggc caggggcacc cacagcgtca
     2281 ccgtggaggc cgccccatat tgggtgcggc ggccacagag tggggtcttc gggccggggg
     2341 agacggcgag gctggactgc gaggtggggg ggaaaccccg accccaaatc caatggagca
     2401 tcaatggggt ccccatcgac gcgctgccgg gcgcggagcg gcggtggctg cggggcggcg
     2461 ctttggtgct tccggagctg cggccgaacg acagcgcggt gctgcagtgc gaggcgagga
     2521 accgccacgg ccgcctattg gccaacgcct tcctgcacgt cgtggagctg ccggtccgaa
     2581 tgctgacggc ggatgagcag cgctacgaag tggtggaaaa ccaaacggtg tttctgcact
     2641 gcagaacctt cggggccccc gcgccaaacg tcgagtggct gacccccact ttggagccgg
     2701 ctctgcagga cgatcgatcc ttcgtgttca ccaatgggag ccttcggctg agtgcggtgc
     2761 ggggggggga cgggggggtc tacacctgca tggcccaaaa cgcccacagc aacggcagcc
     2821 tcacggcgct cctggaggtc agagccccca cccgaatttc ggcccccccc cgaagcgcca
     2881 ccgccaaaaa aggggagacg gtgacctttc actgcggggc gacctttgac cccgccgtga
     2941 cccccgggga gctgcgatgg ctgcgggggg ggcagccgct gcccgacgac ccccggtatt
     3001 cggtggcggc ggagacgctg acggtgtcca acgtggacta tggggacgag gggaccattc
     3061 agtgccgcgc ctccacccct ctcgactccg cggaggccga agcgcagctc agagtcgtgg
     3121 gccgccccgg ccccatccgg gacctccaag tgatggaggt ggacgaacac cgcgtgcgcc
     3181 tcagctggac cccgggggac gaccataaca gccccataga gaagttcgtg gtggaggagg
     3241 aggagggcat cttcagcgcg gggcgcttcg cggagcggct gacggttccg ggggggcagc
     3301 cgtggacgcc ccccctcccg ctgtccccat acgggcggtt ccgcttccgg gtggtggccg
     3361 ttaacgccta cgggaggggg gagcacagcg cccccagcgc ccccatcgag accccccccg
     3421 cggctccgga gcgcaacccg gggggggtcc atggggaggg caatgagacc ggcaacctcg
     3481 tcatcacctg ggagcccctc cccccccagg cctggaacgc cccctgggtt cggtaccgcg
     3541 tgcagtggcg gccattggag gagcccggcg gggggggccc ttcggggggg ttcccgtggg
     3601 ccgaaagcac cgtggacgcc ccccccgtgg tggtgggggg gctccccccg ttcagcccct
     3661 tccagatccg cgtccaggcc gtgaacggag ccgggaaggg accggaagcg acccccggcg
     3721 tggggcacag cggggaggac ctgccgttgg tttaccctga gaatgtgggg gtggaactgc
     3781 tgaacagcag caccgtgcgc gtgagatgga ctttgggggg ggggcccaaa gagctgcggg
     3841 ggcgtctgag gggcttccgg gtgctgtatt ggcgtttggg atgggtgggg gagcgcagtc
     3901 gtcgacaagc cccccccgac cccccccaaa tcccccaaag cccggctgaa gacccccccc
     3961 catttccccc cgtggctctg acagtggggg gggacgcgcg gggggcgctg ctgggggggc
     4021 tgcggccctg gagccgttat cagctgcggg tgttggtctt caacgggagg ggggacggcc
     4081 cccccagcga acccatcgcc ttcgagaccc ccgagggagt tcccggcccc cccgaggagc
     4141 tccgcgtgga gcggttggac gacaccgccc tctccgtcca ttggaaacgg ccgcggcatt
     4201 taaacggagt cctcacggga tatgtactga gataccagca ggtggagccg gcgctcggcc
     4261 tcccccagga ggctccgtac ccccccggga ccctcaatgc gaccctaagg gggctgaatg
     4321 cgcgctcccg ataccggctg gcgctgcgcg cgagcacgcg cgtcgggagc ggccccgccc
     4381 tgcagacggt ggggagcacg aaaccggaac cggccctccc cgctttggag cgcgttttgg
     4441 tgtcggaggt cggggaggat ttcacggtgc tgctgtggag tttggtgcag ccccaggagg
     4501 acgacgtgga gttcgaagtc caattcatga ataaaagcac ggatgagccg tggcgcactt
     4561 cgggccgcgc caactcctct ttaaggcggt accgtctgga ggggctgcgg cccggcaccg
     4621 cctaccgagt ccaattcgtg ggccggaacc gctctgggga aaacgtggcc ttctgggaga
     4681 gcgaagtgca aaccaacggc accgtggtgc gcagcctggt gggggggttt gccaccaagg
     4741 ggtggttcat cggcttcgtc agctccgtgg tgctccttct cctcatcctc ctcatcctct
     4801 gcttcatcaa acgcagcaag gggggcaagt attcggtgaa ggacaaggag gacacgcagg
     4861 tggactctga ggcgcggccc atgaaggatg agacctttgg ggagtacagg tcgttggaga
     4921 gcgaagcgga ggagaagggt tcggcttcgg gttccggcgc cggttccggt gtgggttctc
     4981 cgggtcgggg tccgtgcgcg gcgggcagcg aagacagcct ggcggggtac ggaggcagcg
     5041 gggatgtgca gttcaatgag gatggatcct tcatcgggca gtaccgcgga cccggagccg
     5101 gacccggcag ctccggccct gccagcccct gtgctgggcc ccccctggat ttggtaagta
     5161 agcttggcgc gcctaggcct accatggcta gcctcgagcc aaccactgag gatctgtact
     5221 ttcagagcga taacgatgga tccgaaatcg gtactggctt tccattcgac ccccattatg
     5281 tggaagtcct gggcgagcgc atgcactacg tcgatgttgg tccgcgcgat ggcacccctg
     5341 tgctgttcct gcacggtaac ccgacctcct cctacgtgtg gcgcaacatc atcccgcatg
     5401 ttgcaccgac ccatcgctgc attgctccag acctgatcgg tatgggcaaa tccgacaaac
     5461 cagacctggg ttatttcttc gacgaccacg tccgcttcat ggatgccttc atcgaagccc
     5521 tgggtctgga agaggtcgtc ctggtcattc acgactgggg ctccgctctg ggtttccact
     5581 gggccaagcg caatccagag cgcgtcaaag gtattgcatt tatggagttc atccgcccta
     5641 tcccgacctg ggacgaatgg ccagaatttg cccgcgagac cttccaggcc ttccgcacca
     5701 ccgacgtcgg ccgcaagctg atcatcgatc agaacgtttt tatcgagggt acgctgccga
     5761 tgggtgtcgt ccgcccgctg actgaagtcg agatggacca ttaccgcgag ccgttcctga
     5821 atcctgttga ccgcgagcca ctgtggcgct tcccaaacga gctgccaatc gccggtgagc
     5881 cagcgaacat cgtcgcgctg gtcgaagaat acatggactg gctgcaccag tcccctgtcc
     5941 cgaagctgct gttctggggc accccaggcg ttctgatccc accggccgaa gccgctcgcc
     6001 tggccaaaag cctgcctaac tgcaaggctg tggacatcgg cccgggtctg aatctgctgc
     6061 aagaagacaa cccggacctg atcggcagcg agatcgcgcg ctggctgtct actctggaga
     6121 tttccggtct gtacaagcgg accgtctaat ctagataagt aatgatcata atcagccata
     6181 tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     6241 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     6301 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6361 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccccggg taccgagctc
     6421 gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6481 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6541 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6601 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6661 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6721 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6781 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6841 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6901 aacccgatag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6961 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7021 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7081 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7141 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7201 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7261 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7321 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7381 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7441 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7501 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7561 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7621 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7681 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7741 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7801 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7861 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7921 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7981 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8041 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8101 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8161 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8221 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8281 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8341 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8401 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8461 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8521 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8581 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     8641 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     8701 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     8761 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     8821 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     8881 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     8941 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     9001 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagttg
     9061 ggatctttgc attggcccac ggctctcagg atggggatgc tccccttcag cacccggttc
     9121 cccttggaaa ctgatggtcc tggctctgtg gcatggcagt ggcactgtga ggagccccct
     9181 accagcagca cacagtgggg ttggcactgc cacgctccgg atgccgcgct ctgatccaac
     9241 cccataatca agggaacccg aattgcccca tcattgcccc caccaccccc atcctgccgg
     9301 gccctcacac cccacgctgc cttctggtga cattccccag cccaaaccca cggcttcatg
     9361 gctaccgcgg ggcatttccc attgccgccc cattatcagc tctgcacacc tcccgctgta
     9421 cccatgcctc gtggctgccc ttctttgacg tataatcttc taattaatac ccggccttgt
     9481 caaagtggag cacaaacgtt aattaattcc ccagcaggca ggtaattaac agtgtgactc
     9541 cctttttgct gcgagtgggg ctgatacaga gagatgtggc actatggagc ccacggggtc
     9601 ctggcactgg gtgcccacgg aggtccccat gtgctgcagt gtcaccgcct ccgaggagac
     9661 agtattgtcc ctgcggtgtc cctgcagctc agctctgtcc acagggccac ctccagtttg
     9721 gaggggacac aatgcagccc cgatgcaacc catcctcgca gcatcccagg gacaaagacc
     9781 ccactgcaag accgcacaca gggctgggtc ccgctcccct aatatctaca gtgcttcttg
     9841 catggcccct taatcaatgc agttaatcag catgcgctca tgcaccgctc tggagctgca
     9901 aagcccctcg cagcgctgct caccaacacc gcgcaccgcc ccggcccagc ctgcagcacg
     9961 cgctgcaaac aggaaagaaa caaaatattg cccaaatgta ggcaaaggca ttcggctgcc
    10021 ttgacctccg ccgggccggg ccctgcctga ctcagctcct tactcagcgc tcgcttcctc
    10081 cctccggctg ccaccgccgc agcgcacacc ctgacaaaga gtggccctta acgggctctg
    10141 aggtgcaccc agcagtgcac tcagcagtcc aagggccggc ctggaggttt gcaccgctac
    10201 gtgctgacat tagcattgaa cttggccctg ggtagtgctg caggccgggc ggggtgggtg
    10261 tagagagtgc agcgcgcgtt gcacccggtg ccccttcccc tcccttgcat cccagcaggc
    10321 tgcaccccag caccaggccc gtgcatgcat gctcctggtg ttattgcagc ctggtgcatg
    10381 catgcgtctt gatggtgcag cgctgtgcat gcatcctcct tggtgtgtag cagtctagtg
    10441 catgcatacc cctcggtgtt attgctgctc tgtgcacgca cgctcattgt atcacttcat
    10501 cccagtgcat gcactcacac tggagcgatt gctgctcggt gcacgcacac tcattgtatc
    10561 actgcagctc agtggctgca cgcacaccgg tgttattgct gctcggtgcg tgcatgcaca
    10621 tcagtgtcgc tgcagctcag tgcatgcacg ctcattgccc atcgctatcc ctgcctctcc
    10681 tgctggcgct ccccgggagg tgacttcaag gggaccgcag gaccacctcg ggggtggggg
    10741 gagggctgca cacgcggacc ccgctccccc tccccaacaa agcactgtgg aatcaaaaag
    10801 gggggagggg ggatggaggg gcgcgtcaca cccccg
//
