LOCUS       sequence-319475-       11191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of WT mouse Kif13A with deletion of the 360 
            N-terminal residues.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11191)
  AUTHORS   Frank M, Citarella CG, Quinones GB, Bentley M
  TITLE     A Novel Labeling Strategy Reveals That Myosin Va and Myosin Vb Bind 
            the Same Dendritically Polarized Vesicle Population.
  JOURNAL   Traffic. 2020 Sep 21. doi: 10.1111/tra.12764.
  PUBMED    32959500
REFERENCE   2  (bases 1 to 11191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2020 Sep 21. doi: 10.1111/tra.12764."
COMMENT     Sequence Label: pBa.Halo-KIF13A tail 361-1749
FEATURES             Location/Qualifiers
     source          1..11191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..276
                     /label=chicken beta-actin promoter
     primer_bind     1219..1241
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             1333..1353
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             1369..2253
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     misc_feature    2268..2324
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      4990..4999
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6610..6744
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6647..6666)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6701..6720
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6775..6791)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6775..6791)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6788..6810)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6799..6815
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6823..6829,6830..6847,6848..6853))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6868..6889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7006..7023)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7177..7765)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7257..7276)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7936..8727,8728..8796))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8559..8578
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8797..8901)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8969..8987
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9025..9047)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9147..9166
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9360..9382
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9374..9391
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9375..9391
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      121 cgcgcgccrg gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      181 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      241 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      301 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      361 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      421 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
      481 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
      541 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
      601 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
      661 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
      721 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
      781 acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg
      841 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
      901 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
      961 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     1021 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     1081 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     1141 gccgtcccct tctccatctc cagcctcggg gctgtccgca gggggacggc tgccttcggg
     1201 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggggtt tatatcttcc
     1261 cttctctgtt cctccgcagc ccccaagctt ggcgcgccta ggcctaccat ggctagcctc
     1321 gagccaacca ctgaggatct gtactttcag agcgataacg atggatccga aatcggtact
     1381 ggctttccat tcgaccccca ttatgtggaa gtcctgggcg agcgcatgca ctacgtcgat
     1441 gttggtccgc gcgatggcac ccctgtgctg ttcctgcacg gtaacccgac ctcctcctac
     1501 gtgtggcgca acatcatccc gcatgttgca ccgacccatc gctgcattgc tccagacctg
     1561 atcggtatgg gcaaatccga caaaccagac ctgggttatt tcttcgacga ccacgtccgc
     1621 ttcatggatg ccttcatcga agccctgggt ctggaagagg tcgtcctggt cattcacgac
     1681 tggggctccg ctctgggttt ccactgggcc aagcgcaatc cagagcgcgt caaaggtatt
     1741 gcatttatgg agttcatccg ccctatcccg acctgggacg aatggccaga atttgcccgc
     1801 gagaccttcc aggccttccg caccaccgac gtcggccgca agctgatcat cgatcagaac
     1861 gtttttatcg agggtacgct gccgatgggt gtcgtccgcc cgctgactga agtcgagatg
     1921 gaccattacc gcgagccgtt cctgaatcct gttgaccgcg agccactgtg gcgcttccca
     1981 aacgagctgc caatcgccgg tgagccagcg aacatcgtcg cgctggtcga agaatacatg
     2041 gactggctgc accagtcccc tgtcccgaag ctgctgttct ggggcacccc aggcgttctg
     2101 atcccaccgg ccgaagccgc tcgcctggcc aaaagcctgc ctaactgcaa ggctgtggac
     2161 atcggcccgg gtctgaatct gctgcaagaa gacaacccgg acctgatcgg cagcgagatc
     2221 gcgcgctggc tgtctactct ggagatttcc ggtctgtaca agtactcaga tctcgagctc
     2281 aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atcccaatgc caaggtcatc
     2341 cgagagcttc gagaggaagt ggagaaactg agggagcagc tctcgcaggc tgaggccatg
     2401 aaagcccccg agctaaagga gaagctcgaa gagtctgaga agctgataaa ggagctaacg
     2461 gtgacgtggg aagagaagct gaggaaaaca gaagcgatcg cacaggagag acagcggcag
     2521 ctggaaagca tgggaatctc cctggagacc tctggcatca aggtggggga cgacaagtgc
     2581 tacctcgtca acctgaacgc agaccctgcc ctcaacgagc ttctggttta ctatttaaag
     2641 gatcataccc gggtgggtgc agatacctct caggacatcc agctcttcgg aatagggatt
     2701 cagcccgagc actgtgagat cgacatcgca gcggatggag acatcactct cacgcccaaa
     2761 gaaaatgcaa ggtcctgtgg caatggcacc cttgtctgca gtactaccca gctgtggcac
     2821 ggcgacagga tcttatgggg aaacaaccac ttttttagaa ttaacttgcc taagaggaaa
     2881 cggcgggact ggttgaaaga ctttgagaga gaaaccagtt ccgcggagca cgatctagac
     2941 gctgcgagcg aggcttcctc ggagccagac tacaactacg agttcgcaca gatggaggtc
     3001 atcatgaaaa cactgaacag caatgatcca gttcaaaatg tggttcaggt cctggagaag
     3061 cagtacctgg aagagaagcg gaccgccctg gaggaacagc ggctcatgta cgaacgcgag
     3121 ctggagcagc ttcgccagca gctctctccc gagaggcagc cgcccagcag cgcctcagac
     3181 cgtctggcct acagcagcca gacagcccag cagaaggtga cccagtgggc cgaggagagg
     3241 gatgaactct ttaggcagag cttggccaag cttcgagagc agctggtgaa agccaacact
     3301 ttggtgaggg aagccaactt cctagccgag gagatgagca aactcactga ctaccaagtg
     3361 actctgcaga tccctgcggc caacctcagt gccaacagga agagaggcgc aatcgtcagc
     3421 gagcccgcca tccaagccag gaggaaaggg aagggcaccc aggtctggac cattgagaag
     3481 ctggagaata agctaattga tatgagagac ctttaccaag aatggaaaga aaatgttcct
     3541 gaggccaaga ggctctatgg gaagcgaggt gacccttttt atgaagcgca ggagaatcac
     3601 aacctcattg gtgtggctaa tgtgtttttg gaatgtctct tctgtgatgt gaagcttcaa
     3661 tatgcagtac ccatcatcag ccagcagggg gaggttgccg ggcgcctcca tgtggaggtg
     3721 acgcgcatta caggaaccat tccagaacgc atggcggagg atgactcttc agagaactct
     3781 agtgagagcg ggagtcttga agtggtcgac agcagcggcg aagtcattca ccgggtcaaa
     3841 aagctgacat gcagggtgat aattaaagag gcaaccgggc tacccataag cctctcaaat
     3901 ttcgtcttct gtcagtacac gttctgggac cagtgtgagt ctacagtggc tgctccagtg
     3961 gtggacccag atgtgccctc cccgcagtcc aaggacgcac agtacacggt gaccttctct
     4021 cactgtaagg actacgtggt gactgtcaca gaagagttcc tggagttcat ttctgatggt
     4081 gcgctggcga ttgaagtgtg gggccacagg tgtgctggga acggaagccc catctgggag
     4141 gtggactcgc tccatgcgaa gacaaggaca ttgcatgaca ggtggaatga agtgacccgg
     4201 agaatagagg tgtggatctc cattctggag ctgaatgagc tcggggatta tgccgctgtg
     4261 gagcttcacc aggcgaaaga cgtcaacacc ggcggcgtct tccaactcag acagggtcac
     4321 tcccgtagag tgcaggtcac agtgaaacct gtacagcact cagggacact gccactcatg
     4381 gttgaagcca tcctatcagt atccattggc tgcgtgaccg ccaggtccac caagctgcag
     4441 agagggctgg acagctacca ggaagaagac ttgaattgtg taagagagag atggtccgat
     4501 gcactcatta aacgacggga atacttagac gaacagatta aaaaagtcag caataaaaaa
     4561 gagaaaacag aagacgacat ggagcgggaa gccaggctgg tggagcagtg ggtggggctg
     4621 acagaggaga ggaatgctgt gctggtgccc gcccctggca gcggaatccc cggggcacct
     4681 gctgactggg tcccacctcc tggcatggaa acacacatcc cggttctctt cctggatctg
     4741 aacgcggatg acctcagtgc caatgagcag ctggtgggcc cccatgcgtc cggtgtgaac
     4801 tccatcctgc ccaaggaaca cggcagtcag tttttctacc tgcccatcat aaagcacagt
     4861 gatgacgagg tttcagccac agcctcctgg gactcctctg tgcacgactc tcttcacctg
     4921 aatcgggtca caccgcagaa tgaaaggatc tacctgatcg tgaagaccac cgtgcagctc
     4981 agccacccgg ccgccatgga gctagtgttg cggaagcgga tcgcggccaa tatttataac
     5041 aagcagagtt tcacccagag tttgaagaga cgaatatcac tgatcaatat acgttactcc
     5101 tgcggcgtga cctatgagat agtatccaat atcccaaagg cgaccgagga gattgaggac
     5161 cgggaaaccc tggccctcct ggcagcgcgg agtgagaacg agggcacttt ggatggggag
     5221 acatacatcg agaagtacac gcggggcgtg ctgcaggtgg agaatatcct gagcctggag
     5281 cggctccggc aggctgtcac ggtgaaggaa gcgctttcca ccaaagcgag gcacattcgg
     5341 agaagcctca gtacaccgaa cgttcacaac gtctcctcta gccggccaga tctctctggc
     5401 ttcgatgaag acgataaggg ctggcccgag aaccagttag acgtgtctga ctacagctcc
     5461 agctaccaag acgtcgcgtg ctatgggact ttacccaggg actcacctcg caggagcaag
     5521 gaaggttgtc catcagagaa tcctcatgcc ttaacagtca gcccttttaa agcgttctct
     5581 cctcagccac caaagttctt caaacccctc atgcctgtaa aagaggagca taagaaaagg
     5641 ttggccctgg aagccagacc tctcctaagc caagaggact cggaagagga ggaaaacgag
     5701 ctggaagctc tcagcaggaa gctgatgctt acacagcctt atgtgcccgt ggagttcgct
     5761 gacttcagtg tttacaatgc cagcttggag aacagggagt ggtcctcttc taaggcggac
     5821 ctgacagact cgagagcctt ggagaaagca gtgtcccgta gccctaccac cagcagcctc
     5881 accagtggct acttctccca cagtgcctcc aatgccactc tgtctgacat ggcagtcccc
     5941 tccagcgaca gctcagacca gcttgccgtc tcgacaaagg aggtcgagtg cgccgagccc
     6001 ccgggaccat cacttgcacc ggatgtcaga cgagcttcga atcaggagct tacagaagta
     6061 ggaagaggct cagggaagga tgagacaatc gctgtgccgc tggaggaaaa cagtgcctta
     6121 cccaagggga ccccgtcacc ccagagcatc cctgaggaaa gttccagaat gccatgcagg
     6181 actgcttcat gttcagaact ggatgtcggc cccagcaaag atggccacca agccagagaa
     6241 ttctgcccag gagaggtgac gatagaacac actacaaaca ttttggagga ccattccttc
     6301 acagagttca tgggtgtttc tgatggcaaa gactttgatg gtttggcaga ttgttctgta
     6361 ggggagccct ccaggaggag ggctctaaca aacgaaacag accataaagg tatcccagaa
     6421 agacctccag atgccgaccg gctgcatccc aagatagaga acgaccagga agccacagcc
     6481 actcggtaag ctagataagt aatgatcata atcagccata tcacatctgt agaggtttta
     6541 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     6601 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6661 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6721 aatgtatctt atcatgtctg gatccccggg taccgagctc gaattcgtaa tcatgtcata
     6781 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6841 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6901 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6961 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7021 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7081 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7141 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7201 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7261 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7321 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7381 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7441 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7501 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7561 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7621 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7681 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7741 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7801 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7861 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7921 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7981 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8041 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8101 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8161 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8221 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8281 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8341 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8401 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8461 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8521 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8581 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8641 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8701 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8761 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8821 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8881 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     8941 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     9001 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     9061 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     9121 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     9181 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     9241 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     9301 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     9361 ccagtcacga cgttgtaaaa cgacggccag tgccaagttg ggatctttgc attggcccac
     9421 ggctctcagg atggggatgc tccccttcag cacccggttc cccttggaaa ctgatggtcc
     9481 tggctctgtg gcatggcagt ggcactgtga ggagccccct accagcagca cacagtgggg
     9541 ttggcactgc cacgctccgg atgccgcgct ctgatccaac cccataatca agggaacccg
     9601 aattgcccca tcattgcccc caccaccccc atcctgccgg gccctcacac cccacgctgc
     9661 cttctggtga cattccccag cccaaaccca cggcttcatg gctaccgcgg ggcatttccc
     9721 attgccgccc cattatcagc tctgcacacc tcccgctgta cccatgcctc gtggctgccc
     9781 ttctttgacg tataatcttc taattaatac ccggccttgt caaagtggag cacaaacgtt
     9841 aattaattcc ccagcaggca ggtaattaac agtgtgactc cctttttgct gcgagtgggg
     9901 ctgatacaga gagatgtggc actatggagc ccacggggtc ctggcactgg gtgcccacgg
     9961 aggtccccat gtgctgcagt gtcaccgcct ccgaggagac agtattgtcc ctgcggtgtc
    10021 cctgcagctc agctctgtcc acagggccac ctccagtttg gaggggacac aatgcagccc
    10081 cgatgcaacc catcctcgca gcatcccagg gacaaagacc ccactgcaag accgcacaca
    10141 gggctgggtc ccgctcccct aatatctaca gtgcttcttg catggcccct taatcaatgc
    10201 agttaatcag catgcgctca tgcaccgctc tggagctgca aagcccctcg cagcgctgct
    10261 caccaacacc gcgcaccgcc ccggcccagc ctgcagcacg cgctgcaaac aggaaagaaa
    10321 caaaatattg cccaaatgta ggcaaaggca ttcggctgcc ttgacctccg ccgggccggg
    10381 ccctgcctga ctcagctcct tactcagcgc tcgcttcctc cctccggctg ccaccgccgc
    10441 agcgcacacc ctgacaaaga gtggccctta acgggctctg aggtgcaccc agcagtgcac
    10501 tcagcagtcc aagggccggc ctggaggttt gcaccgctac gtgctgacat tagcattgaa
    10561 cttggccctg ggtagtgctg caggccgggc ggggtgggtg tagagagtgc agcgcgcgtt
    10621 gcacccggtg ccccttcccc tcccttgcat cccagcaggc tgcaccccag caccaggccc
    10681 gtgcatgcat gctcctggtg ttattgcagc ctggtgcatg catgcgtctt gatggtgcag
    10741 cgctgtgcat gcatcctcct tggtgtgtag cagtctagtg catgcatacc cctcggtgtt
    10801 attgctgctc tgtgcacgca cgctcattgt atcacttcat cccagtgcat gcactcacac
    10861 tggagcgatt gctgctcggt gcacgcacac tcattgtatc actgcagctc agtggctgca
    10921 cgcacaccgg tgttattgct gctcggtgcg tgcatgcaca tcagtgtcgc tgcagctcag
    10981 tgcatgcacg ctcattgccc atcgctatcc ctgcctctcc tgctggcgct ccccgggagg
    11041 tgacttcaag gggaccgcag gaccacctcg ggggtggggg gagggctgca cacgcggacc
    11101 ccgctccccc tccccaacaa agcactgtgg aatcaaaaag gggggagggg ggatggaggg
    11161 gcgcgtcaca cccccgcccc acaccctcac c
//
