LOCUS       sequence-319487-       11877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of WT mouse Kif1a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11877)
  AUTHORS   Frank M, Citarella CG, Quinones GB, Bentley M
  TITLE     A Novel Labeling Strategy Reveals That Myosin Va and Myosin Vb Bind 
            the Same Dendritically Polarized Vesicle Population.
  JOURNAL   Traffic. 2020 Sep 21. doi: 10.1111/tra.12764.
  PUBMED    32959500
REFERENCE   2  (bases 1 to 11877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2020 Sep 21. doi: 10.1111/tra.12764."
COMMENT     Sequence Label: pBa.Kif1a-GFP
FEATURES             Location/Qualifiers
     source          1..11877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..276
                     /label=chicken beta-actin promoter
     primer_bind     1219..1241
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      1291..1300
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2273..2282
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5854..5865
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             join(6433..6435,6436..6438,6439..7149)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGGELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6478..6499)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6739..6758)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7086..7107
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7296..7430
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7333..7352)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7387..7406
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7461..7477)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7461..7477)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7474..7496)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7485..7501
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7509..7515,7516..7533,7534..7539))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7554..7575
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7692..7709)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7863..8451)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7943..7962)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8622..9413,9414..9482))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9245..9264
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9483..9587)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9655..9673
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9711..9733)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9833..9852
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10046..10068
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10060..10077
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10061..10077
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggsgggg gggggggggg
      121 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      181 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      241 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      301 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      361 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      421 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
      481 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
      541 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
      601 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
      661 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
      721 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
      781 acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg
      841 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
      901 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
      961 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     1021 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     1081 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     1141 gccgtcccct tctccatctc cagcctcggg gctgtccgca gggggacggc tgccttcggg
     1201 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggggtt tatatcttcc
     1261 cttctctgtt cctccgcagc ccccaagctt gccaccatgg ccggggcctc tgtgaaggtg
     1321 gcggtgcgcg tccgcccctt caactcccgg gaaatgagcc gcgactccaa gtgcatcatt
     1381 cagatgtctg gaaacaccac caccattgtc aaccccaaac agcccaagga gacacccaag
     1441 agcttcagct tcgattactc ctactggtca catacctcgc ccgaggacat caactatgcc
     1501 tctcagaagc aggtctacag ggacattggg gaggagatgc tacagcatgc ctttgagggc
     1561 tacaacgtgt gcatctttgc ctatggacag acaggggctg gcaagtccta caccatgatg
     1621 ggcaaacaag agaaggacca gcaaggcatc atcccacagc tctgtgaaga cctcttctct
     1681 cgaatcaatg acacgaccaa cgacaacatg tcctattctg tggaggtcag ctacatggag
     1741 atttactgtg agcgagtccg cgacctcctg aaccccaaga acaaaggcaa cctgcgtgtg
     1801 agggagcacc ctctgctggg gccttatgtg gaggacctct ccaagctggc tgtgacctcc
     1861 tacaatgaca tccaggacct catggactca gggaacaagg ccaggactgt ggcggccaca
     1921 aacatgaatg agaccagcag ccgttctcac gccgtcttca acatcatctt cacccagaag
     1981 cgccatgatg cggagaccaa catcaccaca gagaaagtga gcaaaatcag cctggtggac
     2041 ctggcgggca gtgaacgagc cgactccacg ggagccaagg gcacacgact caaggaggga
     2101 gccaacatca acaagtcttt gaccaccctg ggaaaggtca tctctgctct cgctgaaatg
     2161 gactctggac ccaataagaa caagaaaaag aagaagacag acttcatccc ttaccgagac
     2221 tcggtattga catggcttct ccgggaaaac ctgggcggca actcaaggac agccatggtg
     2281 gcagccctga gccctgcgga catcaactat gatgagaccc tcagcacact gagatatgca
     2341 gaccgggcca aacagatccg ctgcaacgcc attatcaatg aggaccctaa caacaagctg
     2401 attcgagaac tgaaggatga ggtgaccagg ctgcgagatc tgctgtacgc ccaaggtctt
     2461 ggcgatatca ctgacatgac caatgccctg gtgggcatga gcccctcatc ttcactctca
     2521 gccctgtcca gccgtgcagc ctctgtgtcc agtctccatg agcgcatctt gtttgctcca
     2581 ggcagcgagg aggctattga aaggctgaag gaaacagaga agatcattgc tgaactcaat
     2641 gagacctggg aggagaagct gcggcggaca gaagccatcc ggatggagag ggaagccttg
     2701 ctggctgaga tgggtgtggc catgagggaa gatggtggta ccttgggtgt gttttctccc
     2761 aaaaagactc ctcatcttgt caacctgaat gaggaccctt tgatgtccga atgcctcctt
     2821 tactacatca aagatggcgt caccagagtg ggcagagaag atgcagagag gcggcaaaac
     2881 attgtcctga gtggacactt catcaaagag gagcactgca tcttccggag tgactcccga
     2941 ggaggcggag aagctgtggt gaccctggag ccctgtgaag gagcagacac ctacgttaac
     3001 gggaagaaag tcactgagcc cagcatccta cggtcaggaa accgcatcat catgggaaaa
     3061 agccacgtgt tccggtttaa ccaccctgag caggcccgtc aggagcgtga gcgaacacct
     3121 tgtgcagaga cacctgctga gccagtggac tgggcctttg cccagcggga actgctggag
     3181 aagcaaggca ttgacatgaa gcaggagatg gagcagaggc tccaggagtt ggaggaccag
     3241 taccggaggg agcgggagga ggccacatac cttctggagc agcagaggct ggactatgag
     3301 agtaagctgg aggccctaca gaaacagatg gactctagat actacccaga ggtgaacgag
     3361 gaggaagaag agccagagga tgaagtccaa tggacagaac gagaatgtga gctggcattg
     3421 tgggccttcc ggaagtggaa gtggtaccag tttacttctc tgcgggacct gctgtggggc
     3481 aatgccatct tcctgaagga ggccaacgcc atcagtgtgg agctaaagaa gaaggtccag
     3541 ttccagtttg tcctcctcac ggacaccctg tactccccac tgccgccaga cctactgccc
     3601 ccagaagccg ccaaagaccg agagacacga cccttcccca ggaccattgt ggcagtggag
     3661 gtccaggacc agaagaatgg ggccacccac tactggacac tggagaaact caggcaacgc
     3721 ctggacctga tgcgggaaat gtacgacagg gctgcagagg ttccctccag cgtcgttgag
     3781 gactgtgaca acgtggtgac cggcggggac cccttctatg accgcttccc ctggttccga
     3841 ctggtgggca gggccttcgt atacctgagc aacctgctgt accctgtgcc cctggtgcac
     3901 cgagtggcaa tcgtcagcga aaagggagag gtgaaaggct tcctccgtgt ggcggtgcag
     3961 gccatctcag ctgatgaaga ggctccggat tatggctcgg gtgtccgtca gtcaggaact
     4021 gctaaaatct cctttgatga ccagcatttt gaaaagttcc aatctgagtc ctgccctgtg
     4081 gtggggatgt cccgctcagg aacttcccaa gaggagctgc gcattgtaga aggccagggt
     4141 cagggggctg atgctgggcc ttcagctgat gaagttaaca acaacacctg ttcagctgtg
     4201 cctcctgaag gcctcatgga tagcccagag aaggctgccc tggatgggcc tctggacaca
     4261 gccctggacc acctccgtct aggcagtacc ttcaccttcc gggtgacagt cctgcaggcg
     4321 tccagtatct ctgctgaata cgctgacatc ttctgccagt tcaactttat ccaccgccac
     4381 gatgaggcct tctccactga acccctgaag aacactggca gaggaccccc tcttggcttc
     4441 taccatgtcc aaaatattgc ggtagaggtg accaagtcct tcatcgaata catcaagagc
     4501 cagcccattg tgttcgaggt ctttgggcac taccagcagc acccgttccc acctctttgc
     4561 aaggatgtcc tcagccccct gagaccctca cgtcgccact tccccagggt catgccgtta
     4621 tccaagccag tgcccgccac taagctcagc acgatgacac ggccttcccc aggtccctgc
     4681 cattgcaagt atgacctgct ggtctacttc gagatctgcg agctggaagc caatggcgat
     4741 tacatccctg ctgtggtgga ccaccgtggt ggcatgccgt gcatggggac tttccttctc
     4801 catcagggca tccagagacg gataactgtg acactgttac atgagacagg aagccacatc
     4861 cgctggaagg aagtgcgcga gctagttgtg ggtcgcatcc gaaacacccc agaaactgac
     4921 gaagctctga ttgaccccaa catcttgtcc ctcaacatcc tctcatcagg atacgtccat
     4981 ccagcccagg atgaccggca gtttcttgat tcggacatac ctaggacctt ctaccagttt
     5041 gaagcagcct gggacagttc catgcataac tctctcctgc tgaatcgtgt caccccttac
     5101 cgggagaaaa tctacatgac gctctctgcc tacattgaga tggagaactg tacccagcca
     5161 gctgtcatca ccaaggactt ctgcatggtt ttctactctc gggacgccaa gctacccgcc
     5221 tcacgctcca ttcgcaacct gtttggcagt ggcagcctgc gggccacaga aggcaaccgt
     5281 gtgactggtg tgtatgagct cagcctttgc cacgtggccg acgcaggcag cccagggatg
     5341 cagcgtcggc gccggcgggt gctggacaca tcagtagcct atgtccgggg tgaggagaac
     5401 ctggctggct ggaggccccg aagtgacagc ctgatcctag atcaccagtg ggaactggag
     5461 aaactgagcc tcttacagga ggtggaaaag accaggcact acctgctcct tcgggagaag
     5521 ctggagacga cccagcgccc tggccctgag gtgctgtccc cagcctccag tgaggactct
     5581 gagtcccgta gttcttcagg tgcctcttcc ccattgtcag ccgagggtca gccgtcacct
     5641 ctggaggctc ccaacgagag gcagagggag ctggctgtca agtgcttacg actcctcatg
     5701 cacacattca acagggaata cacccacagc cacgtctgta tcagcgccag cgagagcaag
     5761 ctctctgaga tgtctgtcac cctgatgcgg gacccatcca tgtcccctct gggtgctgct
     5821 acactcactc cctcctccac ttgcccctct ctgatcgaag gacgatacgg tgccactgac
     5881 gtgaggaccc cccagccctg ctcccggccc gccagcccag agcccgagct cctgccagaa
     5941 cttgactcta agaagactcc ctcccctgtt cgggcaacag agacagagaa ggagccccag
     6001 cgactgctgg tccctgacat ccaagagatc cgagtcagcc caatcgtctc caagaaggga
     6061 tacctgcatt tcctggagcc acacacggct ggctgggcca agcgctttgt ggtggtgagg
     6121 cgtccctacg cctacatgta caacagtgac aaggacacgg tggagagatt tgtgctcaac
     6181 ctctccactg cgcaggtgga gtacagcgag gaccagcagg ccatgctcaa gacacccaac
     6241 acattcgctg tgtgcacgga gcaccgaggc atcctgctgc aggccaacag tgacaaggac
     6301 atgcacgact ggctgtatgc cttcaacccg ctcctggctg ggaccatccg gtccaagctc
     6361 tctcgaagga gatctgcgca gatgcgggtc ggcggaggtt ctggaggtgg cagcggcgga
     6421 cctaggccta ccatggtgag caagggcggg gagctgttca ccggggtggt gcccatcctg
     6481 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     6541 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     6601 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     6661 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     6721 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     6781 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     6841 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     6901 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     6961 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     7021 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     7081 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     7141 ctgtacaagc ggacgtctaa tctagattga aagccttccg gaattcattg atcataatca
     7201 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     7261 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     7321 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7381 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cccgggtacc
     7441 gagctcgaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7501 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7561 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7621 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7681 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7741 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7801 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7861 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7921 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7981 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8041 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8101 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8161 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8221 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8281 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8341 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8401 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8461 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8521 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8581 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8641 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8701 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8761 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8821 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8881 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8941 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9001 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9061 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9121 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9181 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9241 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9301 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9361 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9421 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9481 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9541 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9601 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     9661 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     9721 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     9781 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     9841 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     9901 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     9961 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
    10021 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
    10081 aagttgggat ctttgcattg gcccacggct ctcaggatgg ggatgctccc cttcagcacc
    10141 cggttcccct tggaaactga tggtcctggc tctgtggcat ggcagtggca ctgtgaggag
    10201 ccccctacca gcagcacaca gtggggttgg cactgccacg ctccggatgc cgcgctctga
    10261 tccaacccca taatcaaggg aacccgaatt gccccatcat tgcccccacc acccccatcc
    10321 tgccgggccc tcacacccca cgctgccttc tggtgacatt ccccagccca aacccacggc
    10381 ttcatggcta ccgcggggca tttcccattg ccgccccatt atcagctctg cacacctccc
    10441 gctgtaccca tgcctcgtgg ctgcccttct ttgacgtata atcttctaat taatacccgg
    10501 ccttgtcaaa gtggagcaca aacgttaatt aattccccag caggcaggta attaacagtg
    10561 tgactccctt tttgctgcga gtggggctga tacagagaga tgtggcacta tggagcccac
    10621 ggggtcctgg cactgggtgc ccacggaggt ccccatgtgc tgcagtgtca ccgcctccga
    10681 ggagacagta ttgtccctgc ggtgtccctg cagctcagct ctgtccacag ggccacctcc
    10741 agtttggagg ggacacaatg cagccccgat gcaacccatc ctcgcagcat cccagggaca
    10801 aagaccccac tgcaagaccg cacacagggc tgggtcccgc tcccctaata tctacagtgc
    10861 ttcttgcatg gccccttaat caatgcagtt aatcagcatg cgctcatgca ccgctctgga
    10921 gctgcaaagc ccctcgcagc gctgctcacc aacaccgcgc accgccccgg cccagcctgc
    10981 agcacgcgct gcaaacagga aagaaacaaa atattgccca aatgtaggca aaggcattcg
    11041 gctgccttga cctccgccgg gccgggccct gcctgactca gctccttact cagcgctcgc
    11101 ttcctccctc cggctgccac cgccgcagcg cacaccctga caaagagtgg cccttaacgg
    11161 gctctgaggt gcacccagca gtgcactcag cagtccaagg gccggcctgg aggtttgcac
    11221 cgctacgtgc tgacattagc attgaacttg gccctgggta gtgctgcagg ccgggcgggg
    11281 tgggtgtaga gagtgcagcg cgcgttgcac ccggtgcccc ttcccctccc ttgcatccca
    11341 gcaggctgca ccccagcacc aggcccgtgc atgcatgctc ctggtgttat tgcagcctgg
    11401 tgcatgcatg cgtcttgatg gtgcagcgct gtgcatgcat cctccttggt gtgtagcagt
    11461 ctagtgcatg catacccctc ggtgttattg ctgctctgtg cacgcacgct cattgtatca
    11521 cttcatccca gtgcatgcac tcacactgga gcgattgctg ctcggtgcac gcacactcat
    11581 tgtatcactg cagctcagtg gctgcacgca caccggtgtt attgctgctc ggtgcgtgca
    11641 tgcacatcag tgtcgctgca gctcagtgca tgcacgctca ttgcccatcg ctatccctgc
    11701 ctctcctgct ggcgctcccc gggaggtgac ttcaagggga ccgcaggacc acctcggggg
    11761 tggggggagg gctgcacacg cggaccccgc tccccctccc caacaaagca ctgtggaatc
    11821 aaaaaggggg gaggggggat ggaggggcgc gtcacacccc cgccccacac cctcacc
//
