LOCUS       sequence-319491-       13527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration Vector, assembled with pHIE048 (TUPV1), pJM466 (TUPV2), 
            pJM452 (TUPV3), pPD613.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13527)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 13527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pHIE056 ZF43x6-S mKate2 EF1a VP16-ZF43 EF1a
            EB...
FEATURES             Location/Qualifiers
     source          1..13527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1031..1068
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1079..1678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1079..1098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1575..1595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1791..1810)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1856..1911
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1910..1929)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     regulatory      3217..3226
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3223..3921
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    3972..4196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5245..6427
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5476..6418
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      6528..6537
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6537..6602
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6609..6629
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7226..7450
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8499..9681
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8730..9672
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      9782..9791
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9788..9790,9791..9793,9794..10504)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(9833..9854)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             10532..10588
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10601..10999
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    11050..11274
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(12133..12942)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     12856..12875
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     12934..12953
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(join(13101..13527,1..162))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13181..13200)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
       61 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      241 ttatcaaaaa ggatcttcac ctagatcctt tctcgagctt tcggagtgag atggagtggc
      301 cactgtggaa ctgggcctag catcgcgata agctctagag ggacagcccc cccccaaagc
      361 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
      421 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
      481 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
      541 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
      601 cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt gatatcgaat
      661 tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
      721 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
      781 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
      841 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
      901 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
      961 gcgcgcctgg tggcgaacga tcaacagacg ggagatctac tattgggaca gcagagatcc
     1021 agttggtaat ggcttgtcga cgacggcggt ctccgtcgtc aggatcatag gtgccaccat
     1081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     1141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     1201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1561 gacatccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc
     1681 ttccactgga ttgtacaatt accaactgaa ttcactcctc aggtgcaggc tgcctatcag
     1741 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     1801 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     1861 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     1921 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     1981 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     2041 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     2101 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     2161 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     2221 cttctcttat ggagatccgg gcctagcatc gcgataagct ctagagggac agcccccccc
     2281 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     2341 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     2401 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     2461 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     2521 gaatgacaga atcatagaac ggcctgcgta cggatggggc tctagctcta gagcttgata
     2581 tcgaattcct gcagccccgc tagagggaca gccccccccc aaagccccca gggatgtaat
     2641 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     2701 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     2761 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     2821 ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg
     2881 gcctgggcgc gcctggtggc gaacgatcaa cagacgggag attgccaagg tcttgacaaa
     2941 cgagtgctag tacaagatcg cgtcgacgga tcgggagatc tgaattcgga tccagagtga
     3001 ggactaccta tagaagtaag agtgaggaca atcgtataca agtagatcca gagtgaggac
     3061 tacctataga agtaagagtg aggacaatcg tatacaagta gatccagagt gaggactacc
     3121 tatagaagta agagtgagga caatcgtata caagtagatc cctctagagg gtatataatg
     3181 ggggccacta gtctactacc agagctcatc gctagcgcca ccatggtgag cgagctgatt
     3241 aaggagaaca tgcacatgaa gctgtatatg gagggcaccg tgaacaacca ccacttcaag
     3301 tgtacctccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggcg
     3361 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc
     3421 agcaaaacct tcatcaacca cacccagggc atccccgact tctttaagca gtccttcccc
     3481 gagggcttca catgggagag agtcaccaca tacgaagatg ggggcgtgct gaccgctacc
     3541 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
     3601 ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc ctccaccgaa
     3661 accctgtacc ccgctgacgg cggcctggaa ggcagagccg acatggccct gaagctcgtg
     3721 ggcgggggcc acctgatctg caacttgaaa accacataca gatccaagaa acccgctaag
     3781 aacctcaaga tgcccggcgt ctactatgtg gacaggagac tggaaagaat caaggaggcc
     3841 gacaaagaaa cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct
     3901 agcaaactgg ggcacagatg agcggccgct cgagtctaga gggcccgttt aaacccgctg
     3961 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     4021 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4081 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     4141 gggggaggat tgggaagtca atagcaggca tgctggggat gcggtgggct ctatggcttc
     4201 tgaggcaggt cgagggtaca tgcgccttac tccttgtgag cgcatcgcga taagctctag
     4261 agggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg
     4321 cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg
     4381 gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac
     4441 gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa aagctttagg
     4501 ctgaaagaga gatttagaat gacagaatca tagaacggcc tgcgtacgga tggggctcta
     4561 gctctagagc ttgatatcga attcctgcag ccccgctaga gggacagccc ccccccaaag
     4621 cccccaggga tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc
     4681 tccgctccgg tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg
     4741 ggcacgggga aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc
     4801 ctgcagacac ctggggggat acggggaaaa agctttaggc tgaaagagag atttagaatg
     4861 acagaatcat agaacggcct gggcgcgcct ggtggcgaac gatcaacaga cgggagaggg
     4921 caaaaccgat gttgacggac taatcctgac aagatctggg gaggtcgctg agtagtgcgc
     4981 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     5041 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gtgagtaatt
     5101 catacaaaag gactcgcccc tgccttgggg aatcccaggg accgtcgtta aactcccact
     5161 aacgtagaac ccagagatcg ctgcgttccc gccccctcac ccgcccgctc tcgtcatcac
     5221 tgaggtggag aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg
     5281 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
     5341 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     5401 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     5461 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
     5521 tatggccctt gcgtgccttg aattacttcc acgcccctgg ctgcagtacg tgattcttga
     5581 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
     5641 ttcgcctcgt gcttgagttg aggcctggct tgggcgctgg ggccgccgcg tgcgaatctg
     5701 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
     5761 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
     5821 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
     5881 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     5941 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     6001 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     6061 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     6121 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     6181 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
     6241 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     6301 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     6361 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     6421 gtcgtgagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     6481 gacctccata gaagagaccg ggaccgatcc aaattctcga ggctagcgcc accatggatt
     6541 ataaggatca cgatggcgac tacaaggacc acgatattga ctacaaagac gatgacgata
     6601 aaatggctcc taagaaaaag cgcaaagtcg gtatccatgg cgttcccgtc gacggtggac
     6661 tgcaggcccc accaaccgat gtttctttag gtgatgagtt acatttggac ggtgaggacg
     6721 tagctatggc acatgcagat gccttagacg attttgactt ggatatgtta ggagatggag
     6781 attcaccagg acctggtttc acaccacacg attcagctcc atacggtgct ttggacatgg
     6841 ccgatttcga atttgaacag atgtttactg atgccttggg tatagacgaa tacggtggtg
     6901 gagctagacc cggggagcgc cccttccagt gtcgcatttg catgcggaac ttttcgcgcc
     6961 aggacaggct tgacaggcat acccgtactc ataccggtga aaaaccgttt cagtgtcgga
     7021 tctgtatgcg aaatttctcc cagaaggagc acttggcggg gcatctacgt acgcacaccg
     7081 gcgagaagcc attccaatgc cgaatatgca tgcgcaactt cagtcgccgc gacaacctga
     7141 accggcacct aaaaacccac ctgagaggat cttaagcggc cgctcgagtc tagagggccc
     7201 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     7261 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     7321 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     7381 gggcaggaca gcaaggggga ggattgggaa gtcaatagca ggcatgctgg ggatgcggtg
     7441 ggctctatgg cttctgaggc aggtcgaaga actatttcct ggctgttacg cgagcgcatc
     7501 gcgataagct ctagagggac agcccccccc caaagccccc agggatgtaa ttacgtccct
     7561 cccccgctag ggggcagcag cgagccgccc ggggctccgc tccggtccgg cgctcccccc
     7621 gcatccccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc
     7681 gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg gggatacggg
     7741 gaaaaagctt taggctgaaa gagagattta gaatgacaga atcatagaac ggcctgcgta
     7801 cggatggggc tctagctcta gagcttgata tcgaattcct gcagccccgc tagagggaca
     7861 gccccccccc aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc
     7921 gagccgcccg gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt
     7981 gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc
     8041 gctgctcttt gagcctgcag acacctgggg ggatacgggg aaaaagcttt aggctgaaag
     8101 agagatttag aatgacagaa tcatagaacg gcctgggcgc gcctggtggc gaacgatcaa
     8161 cagacgggag aggactaaat agtagttcag acgccgttaa gcgcaagatc tggggaggtc
     8221 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     8281 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     8341 acgcgtgagt aattcataca aaaggactcg cccctgcctt ggggaatccc agggaccgtc
     8401 gttaaactcc cactaacgta gaacccagag atcgctgcgt tcccgccccc tcacccgccc
     8461 gctctcgtca tcactgaggt ggagaagagc atgcgtgagg ctccggtgcc cgtcagtggg
     8521 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     8581 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     8641 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     8701 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     8761 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacgccc ctggctgcag
     8821 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
     8881 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcttgggcg ctggggccgc
     8941 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
     9001 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
     9061 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
     9121 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     9181 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     9241 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     9301 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     9361 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     9421 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
     9481 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     9541 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     9601 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     9661 cttccatttc aggtgtcgtg agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     9721 atccacgctg ttttgacctc catagaagag aacgggaccg atccaaattc tcgaggctag
     9781 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     9841 gctggacggc gacgtaaacg gccacaagtt cagcgtgagg ggcgagggcg agggcgatgc
     9901 caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     9961 gcccaccctc gtgaccaccc tgagccacgg cgtgcagtgc ttcgcccgct accccgacca
    10021 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
    10081 catcttcttc aaggacgacg gcacctacaa gacccgcgcc gaggtgaagt tcgagggcga
    10141 caccctagtg aaccgcatcg agctgaaggg cgtcgacttc aaggaggacg gcaacatcct
    10201 ggggcacaag ctggagtaca acttcaacag ccacaacatc tatatcatgg ccgtcaagca
    10261 gaagaacggc atcaaggtga acttcaagat ccgccacaac gtggaggacg gcagcgtgca
    10321 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
    10381 cagccactac ctgagcaccc agtccgtgct gagcaaagac cccaacgaga agcgcgatca
    10441 catggtcctg ctggagttcc gcaccgccgc cgggatcact ctcggcatgg acgagctgta
    10501 caaggaattc ggtagcaagc ttggtagtgg agcaactaac ttttccctgc tcaaacaagc
    10561 cggggatgta gaggagaacc ccggccccgc cccccttaag atggccaagc ctttgtctca
    10621 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
    10681 ggactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
    10741 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
    10801 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
    10861 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
    10921 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
    10981 ttatgtgtgg gagggctaag cggccgctcg agtctagagg gcccgtttaa acccgctgat
    11041 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
    11101 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
    11161 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
    11221 gggaggattg ggaagtcaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
    11281 aggcaggtcg aagagactgc ccgacacatc ttagtgagcg catcgcgata agctctagag
    11341 ggacagcccc cccccaaagc ccccagggat gtaattacgt ccctcccccg ctagggggca
    11401 gcagcgagcc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc
    11461 agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc
    11521 ttctcgctgc tctttgagcc tgcagacacc tggggggata cggggaaaaa gctttaggct
    11581 gaaagagaga tttagaatga cagaatcata gaacggcctg cgtacggatg gggctctagc
    11641 tctagagctt gatatcgaat tcctgcagcc ccgctagagg gacagccccc ccccaaagcc
    11701 cccagggatg taattacgtc cctcccccgc tagggggcag cagcgagccg cccggggctc
    11761 cgctccggtc cggcgctccc cccgcatccc cgagccggca gcgtgcgggg acagcccggg
    11821 cacggggaag gtggcacggg atcgctttcc tctgaacgct tctcgctgct ctttgagcct
    11881 gcagacacct ggggggatac ggggaaaaag ctttaggctg aaagagagat ttagaatgac
    11941 agaatcatag aacggcctgg gcgcgcctgg tggcgaacga tcaacagacg ggagaggtta
    12001 cgaggatgca catgtgaccg agggatggaa cgacgcgtaa ctcacgttaa gggattttgg
    12061 tcatgagctt gcgccgtccc gtcaagtcag cgtaatgctc tgccagtgtt acaaccaatt
    12121 aaccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc
    12181 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
    12241 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac
    12301 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc
    12361 atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg
    12421 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt
    12481 cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca
    12541 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc
    12601 tgaatcagga tattcttcta atacctggaa tgctgttttt ccggggatcg cagtggtgag
    12661 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc
    12721 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc
    12781 atgtttcaga aacaactctg gcgcatcggg cttcccatac aagcgataga ttgtcgcacc
    12841 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
    12901 atttaatcgc ggcctcgacg tttcccgttg aatatggctc ataacacccc ttgtattact
    12961 gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta
    13021 acatcagaga ttttgagaca cgggccatgc tagctccagc aaaaggccag gaaccgtaaa
    13081 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    13141 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    13201 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    13261 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    13321 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    13381 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    13441 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    13501 gagttcttga agtggtggcc taactac
//
